Experiment: Def Nalidixic 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0696 and Psest_0697 are separated by 21 nucleotides Psest_0697 and Psest_0698 are separated by 103 nucleotides Psest_0698 and Psest_0699 are separated by 138 nucleotides
Psest_0696: Psest_0696 - hypothetical protein, at 760,446 to 761,339
_0696
Psest_0697: Psest_0697 - ribosomal protein L9, at 761,361 to 761,807
_0697
Psest_0698: Psest_0698 - replicative DNA helicase, at 761,911 to 763,305
_0698
Psest_0699: Psest_0699 - alanine racemase, at 763,444 to 764,520
_0699
Position (kb) 761
762
763
764 Strain fitness (log2 ratio) -2
-1
0
1
2 at 760.986 kb on + strand, within Psest_0696 at 760.987 kb on - strand, within Psest_0696 at 761.131 kb on + strand, within Psest_0696 at 761.205 kb on + strand, within Psest_0696 at 761.205 kb on + strand, within Psest_0696 at 761.205 kb on + strand, within Psest_0696 at 761.205 kb on + strand, within Psest_0696 at 761.206 kb on - strand, within Psest_0696 at 761.543 kb on + strand, within Psest_0697 at 761.740 kb on - strand, within Psest_0697 at 761.746 kb on + strand, within Psest_0697 at 761.746 kb on + strand, within Psest_0697 at 761.746 kb on + strand, within Psest_0697 at 761.746 kb on + strand, within Psest_0697 at 761.746 kb on + strand, within Psest_0697 at 761.746 kb on + strand, within Psest_0697 at 761.747 kb on - strand, within Psest_0697 at 761.890 kb on + strand at 763.286 kb on - strand at 763.286 kb on - strand at 763.286 kb on - strand at 763.286 kb on - strand at 763.405 kb on + strand at 763.405 kb on + strand at 763.406 kb on - strand at 763.406 kb on - strand at 763.429 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Nalidixic 0.001 mg/ml remove 760,986 + Psest_0696 0.60 +0.0 760,987 - Psest_0696 0.61 -0.3 761,131 + Psest_0696 0.77 +0.5 761,205 + Psest_0696 0.85 +0.8 761,205 + Psest_0696 0.85 -0.5 761,205 + Psest_0696 0.85 +1.4 761,205 + Psest_0696 0.85 +1.3 761,206 - Psest_0696 0.85 -1.1 761,543 + Psest_0697 0.41 -1.0 761,740 - Psest_0697 0.85 +0.1 761,746 + Psest_0697 0.86 +2.7 761,746 + Psest_0697 0.86 +0.4 761,746 + Psest_0697 0.86 +0.9 761,746 + Psest_0697 0.86 +2.0 761,746 + Psest_0697 0.86 +1.4 761,746 + Psest_0697 0.86 +1.6 761,747 - Psest_0697 0.86 +0.6 761,890 + +2.0 763,286 - -1.1 763,286 - +0.5 763,286 - -2.3 763,286 - -1.5 763,405 + +0.1 763,405 + -0.7 763,406 - -2.3 763,406 - -1.6 763,429 + -2.3
Or see this region's nucleotide sequence