Experiment: Def Nalidixic 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0682 and Psest_0683 are separated by 92 nucleotides Psest_0683 and Psest_0684 are separated by 11 nucleotides Psest_0684 and Psest_0685 are separated by 98 nucleotides
Psest_0682: Psest_0682 - tRNA dimethylallyltransferase, at 745,683 to 746,654
_0682
Psest_0683: Psest_0683 - RNA chaperone Hfq, at 746,747 to 747,001
_0683
Psest_0684: Psest_0684 - GTP-binding protein HflX, at 747,013 to 748,314
_0684
Psest_0685: Psest_0685 - HflK protein, at 748,413 to 749,591
_0685
Position (kb) 747
748
749 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 746.625 kb on + strand at 746.706 kb on + strand at 746.714 kb on + strand at 747.332 kb on + strand, within Psest_0684 at 747.332 kb on + strand, within Psest_0684 at 747.414 kb on + strand, within Psest_0684 at 747.525 kb on + strand, within Psest_0684 at 747.525 kb on + strand, within Psest_0684 at 747.525 kb on + strand, within Psest_0684 at 747.628 kb on + strand, within Psest_0684 at 747.961 kb on + strand, within Psest_0684 at 747.961 kb on + strand, within Psest_0684 at 747.961 kb on + strand, within Psest_0684 at 747.961 kb on + strand, within Psest_0684 at 748.312 kb on + strand at 748.388 kb on + strand at 748.388 kb on + strand at 748.394 kb on + strand at 748.394 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Nalidixic 0.001 mg/ml remove 746,625 + +0.8 746,706 + -0.4 746,714 + -2.3 747,332 + Psest_0684 0.25 -2.3 747,332 + Psest_0684 0.25 -3.0 747,414 + Psest_0684 0.31 -1.7 747,525 + Psest_0684 0.39 -0.0 747,525 + Psest_0684 0.39 -0.9 747,525 + Psest_0684 0.39 -1.2 747,628 + Psest_0684 0.47 -1.7 747,961 + Psest_0684 0.73 -0.0 747,961 + Psest_0684 0.73 -0.2 747,961 + Psest_0684 0.73 -0.2 747,961 + Psest_0684 0.73 +0.2 748,312 + -1.0 748,388 + +0.5 748,388 + +0.3 748,394 + -0.6 748,394 + -0.8
Or see this region's nucleotide sequence