Experiment: Def Nalidixic 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0521 and Psest_0522 are separated by 62 nucleotides Psest_0522 and Psest_0523 are separated by 93 nucleotides Psest_0523 and Psest_0524 are separated by 37 nucleotides Psest_0524 and Psest_0525 are separated by 43 nucleotides
Psest_0521: Psest_0521 - lytic murein transglycosylase, at 573,615 to 574,913
_0521
Psest_0522: Psest_0522 - Uncharacterized protein conserved in bacteria, at 574,976 to 575,134
_0522
Psest_0523: Psest_0523 - DNA polymerase III, delta subunit, at 575,228 to 576,256
_0523
Psest_0524: Psest_0524 - Rare lipoprotein B, at 576,294 to 576,899
_0524
Psest_0525: Psest_0525 - leucyl-tRNA synthetase, eubacterial and mitochondrial family, at 576,943 to 579,549
_0525
Position (kb) 575
576
577 Strain fitness (log2 ratio) -2
-1
0
1
2 at 574.231 kb on + strand, within Psest_0521 at 574.231 kb on + strand, within Psest_0521 at 574.231 kb on + strand, within Psest_0521 at 574.231 kb on + strand, within Psest_0521 at 574.231 kb on + strand, within Psest_0521 at 574.231 kb on + strand, within Psest_0521 at 574.231 kb on + strand, within Psest_0521 at 574.231 kb on + strand, within Psest_0521 at 574.231 kb on + strand, within Psest_0521 at 574.231 kb on + strand, within Psest_0521 at 574.231 kb on + strand, within Psest_0521 at 574.231 kb on + strand, within Psest_0521 at 574.231 kb on + strand, within Psest_0521 at 574.231 kb on + strand, within Psest_0521 at 574.231 kb on + strand, within Psest_0521 at 574.232 kb on - strand, within Psest_0521 at 574.232 kb on - strand, within Psest_0521 at 574.232 kb on - strand, within Psest_0521 at 574.232 kb on - strand, within Psest_0521 at 574.232 kb on - strand, within Psest_0521 at 574.232 kb on - strand, within Psest_0521 at 574.232 kb on - strand, within Psest_0521 at 574.232 kb on - strand, within Psest_0521 at 574.232 kb on - strand, within Psest_0521 at 574.232 kb on - strand, within Psest_0521 at 574.232 kb on - strand, within Psest_0521 at 574.232 kb on - strand, within Psest_0521 at 574.232 kb on - strand, within Psest_0521 at 574.232 kb on - strand, within Psest_0521 at 574.571 kb on - strand, within Psest_0521 at 574.677 kb on - strand, within Psest_0521 at 574.677 kb on - strand, within Psest_0521 at 574.772 kb on + strand, within Psest_0521 at 574.909 kb on + strand at 574.909 kb on + strand at 574.909 kb on + strand at 574.909 kb on + strand at 574.909 kb on + strand at 574.909 kb on + strand at 574.909 kb on + strand at 574.909 kb on + strand at 574.909 kb on + strand at 574.909 kb on + strand at 574.910 kb on - strand at 574.910 kb on - strand at 574.910 kb on - strand at 574.910 kb on - strand at 574.910 kb on - strand at 574.910 kb on - strand at 574.951 kb on - strand at 574.951 kb on - strand at 574.951 kb on - strand at 575.177 kb on + strand at 575.178 kb on - strand at 575.178 kb on - strand at 576.295 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Nalidixic 0.001 mg/ml remove 574,231 + Psest_0521 0.47 -0.6 574,231 + Psest_0521 0.47 +0.2 574,231 + Psest_0521 0.47 -0.4 574,231 + Psest_0521 0.47 +0.0 574,231 + Psest_0521 0.47 -1.2 574,231 + Psest_0521 0.47 -1.0 574,231 + Psest_0521 0.47 -0.8 574,231 + Psest_0521 0.47 -0.9 574,231 + Psest_0521 0.47 -2.2 574,231 + Psest_0521 0.47 -1.2 574,231 + Psest_0521 0.47 -1.2 574,231 + Psest_0521 0.47 -1.2 574,231 + Psest_0521 0.47 -0.9 574,231 + Psest_0521 0.47 -1.1 574,231 + Psest_0521 0.47 -0.2 574,232 - Psest_0521 0.47 -0.6 574,232 - Psest_0521 0.47 -0.3 574,232 - Psest_0521 0.47 -0.6 574,232 - Psest_0521 0.47 -0.7 574,232 - Psest_0521 0.47 +0.1 574,232 - Psest_0521 0.47 +0.7 574,232 - Psest_0521 0.47 +0.2 574,232 - Psest_0521 0.47 -0.6 574,232 - Psest_0521 0.47 -1.7 574,232 - Psest_0521 0.47 -0.3 574,232 - Psest_0521 0.47 -2.2 574,232 - Psest_0521 0.47 -0.8 574,232 - Psest_0521 0.47 -1.0 574,232 - Psest_0521 0.47 -0.5 574,571 - Psest_0521 0.74 -0.1 574,677 - Psest_0521 0.82 -0.8 574,677 - Psest_0521 0.82 +2.1 574,772 + Psest_0521 0.89 +0.5 574,909 + -1.0 574,909 + -0.7 574,909 + -0.5 574,909 + -0.1 574,909 + -0.7 574,909 + -0.6 574,909 + -0.2 574,909 + +0.1 574,909 + +0.6 574,909 + -0.2 574,910 - +0.6 574,910 - -0.3 574,910 - -0.5 574,910 - -0.3 574,910 - +0.7 574,910 - -0.4 574,951 - +0.1 574,951 - -0.0 574,951 - +0.6 575,177 + -0.5 575,178 - +0.0 575,178 - +0.3 576,295 + -0.2
Or see this region's nucleotide sequence