Experiment: Def Nalidixic 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2217 and Psest_2218 are separated by 13 nucleotides Psest_2218 and Psest_2219 overlap by 4 nucleotides Psest_2219 and Psest_2220 overlap by 1 nucleotides
Psest_2217: Psest_2217 - Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes, at 2,366,006 to 2,367,154
_2217
Psest_2218: Psest_2218 - Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit, at 2,367,168 to 2,368,142
_2218
Psest_2219: Psest_2219 - pyruvate dehydrogenase E1 component, alpha subunit, at 2,368,139 to 2,369,131
_2219
Psest_2220: Psest_2220 - Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases, at 2,369,131 to 2,370,888
_2220
Position (kb)
2368
2369
2370 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2367.170 kb on - strand at 2367.610 kb on + strand, within Psest_2218 at 2367.610 kb on + strand, within Psest_2218 at 2367.611 kb on - strand, within Psest_2218 at 2368.027 kb on + strand, within Psest_2218 at 2368.124 kb on - strand at 2368.161 kb on + strand at 2368.161 kb on + strand at 2368.161 kb on + strand at 2368.162 kb on - strand at 2368.162 kb on - strand at 2368.308 kb on + strand, within Psest_2219 at 2368.308 kb on + strand, within Psest_2219 at 2368.308 kb on + strand, within Psest_2219 at 2368.309 kb on - strand, within Psest_2219 at 2368.362 kb on + strand, within Psest_2219 at 2368.402 kb on - strand, within Psest_2219 at 2368.402 kb on - strand, within Psest_2219 at 2368.422 kb on + strand, within Psest_2219 at 2368.857 kb on + strand, within Psest_2219 at 2368.858 kb on - strand, within Psest_2219 at 2368.858 kb on - strand, within Psest_2219 at 2368.920 kb on + strand, within Psest_2219 at 2368.968 kb on - strand, within Psest_2219 at 2368.992 kb on + strand, within Psest_2219 at 2368.992 kb on + strand, within Psest_2219 at 2368.992 kb on + strand, within Psest_2219 at 2368.992 kb on + strand, within Psest_2219 at 2368.992 kb on + strand, within Psest_2219 at 2368.992 kb on + strand, within Psest_2219 at 2368.992 kb on + strand, within Psest_2219 at 2368.993 kb on - strand, within Psest_2219 at 2368.993 kb on - strand, within Psest_2219 at 2368.993 kb on - strand, within Psest_2219 at 2368.993 kb on - strand, within Psest_2219 at 2368.993 kb on - strand, within Psest_2219 at 2368.993 kb on - strand, within Psest_2219 at 2368.993 kb on - strand, within Psest_2219 at 2369.505 kb on - strand, within Psest_2220 at 2369.547 kb on - strand, within Psest_2220 at 2369.547 kb on - strand, within Psest_2220 at 2369.594 kb on + strand, within Psest_2220 at 2369.595 kb on - strand, within Psest_2220 at 2369.656 kb on + strand, within Psest_2220 at 2369.961 kb on - strand, within Psest_2220 at 2370.071 kb on + strand, within Psest_2220 at 2370.071 kb on + strand, within Psest_2220 at 2370.113 kb on + strand, within Psest_2220 at 2370.113 kb on + strand, within Psest_2220 at 2370.113 kb on + strand, within Psest_2220 at 2370.113 kb on + strand, within Psest_2220 at 2370.113 kb on + strand, within Psest_2220 at 2370.113 kb on + strand, within Psest_2220 at 2370.113 kb on + strand, within Psest_2220 at 2370.113 kb on + strand, within Psest_2220 at 2370.113 kb on + strand, within Psest_2220 at 2370.113 kb on + strand, within Psest_2220 at 2370.114 kb on - strand, within Psest_2220 at 2370.114 kb on - strand, within Psest_2220 at 2370.114 kb on - strand, within Psest_2220 at 2370.114 kb on - strand, within Psest_2220 at 2370.114 kb on - strand, within Psest_2220 at 2370.114 kb on - strand, within Psest_2220 at 2370.114 kb on - strand, within Psest_2220
Per-strain Table
Position Strand Gene LocusTag Fraction Def Nalidixic 0.001 mg/ml remove 2,367,170 - -0.2 2,367,610 + Psest_2218 0.45 -1.9 2,367,610 + Psest_2218 0.45 +0.4 2,367,611 - Psest_2218 0.45 +1.4 2,368,027 + Psest_2218 0.88 -0.5 2,368,124 - +1.8 2,368,161 + -0.7 2,368,161 + -0.2 2,368,161 + +1.4 2,368,162 - +1.4 2,368,162 - -0.0 2,368,308 + Psest_2219 0.17 +0.7 2,368,308 + Psest_2219 0.17 +1.2 2,368,308 + Psest_2219 0.17 +0.2 2,368,309 - Psest_2219 0.17 +1.0 2,368,362 + Psest_2219 0.22 -0.2 2,368,402 - Psest_2219 0.26 -0.4 2,368,402 - Psest_2219 0.26 +1.4 2,368,422 + Psest_2219 0.28 +0.8 2,368,857 + Psest_2219 0.72 -0.4 2,368,858 - Psest_2219 0.72 +1.2 2,368,858 - Psest_2219 0.72 +2.4 2,368,920 + Psest_2219 0.79 +0.2 2,368,968 - Psest_2219 0.83 +0.4 2,368,992 + Psest_2219 0.86 +0.2 2,368,992 + Psest_2219 0.86 -0.4 2,368,992 + Psest_2219 0.86 +0.4 2,368,992 + Psest_2219 0.86 +1.2 2,368,992 + Psest_2219 0.86 -0.2 2,368,992 + Psest_2219 0.86 +0.4 2,368,992 + Psest_2219 0.86 -1.7 2,368,993 - Psest_2219 0.86 +1.4 2,368,993 - Psest_2219 0.86 +0.6 2,368,993 - Psest_2219 0.86 +1.8 2,368,993 - Psest_2219 0.86 +2.4 2,368,993 - Psest_2219 0.86 -0.9 2,368,993 - Psest_2219 0.86 +2.4 2,368,993 - Psest_2219 0.86 +0.4 2,369,505 - Psest_2220 0.21 -0.3 2,369,547 - Psest_2220 0.24 +0.8 2,369,547 - Psest_2220 0.24 -0.2 2,369,594 + Psest_2220 0.26 +1.0 2,369,595 - Psest_2220 0.26 +1.1 2,369,656 + Psest_2220 0.30 -0.3 2,369,961 - Psest_2220 0.47 -1.6 2,370,071 + Psest_2220 0.53 +1.0 2,370,071 + Psest_2220 0.53 +2.4 2,370,113 + Psest_2220 0.56 +0.9 2,370,113 + Psest_2220 0.56 -0.2 2,370,113 + Psest_2220 0.56 -0.2 2,370,113 + Psest_2220 0.56 -0.6 2,370,113 + Psest_2220 0.56 +0.1 2,370,113 + Psest_2220 0.56 +0.4 2,370,113 + Psest_2220 0.56 -1.7 2,370,113 + Psest_2220 0.56 -1.3 2,370,113 + Psest_2220 0.56 -0.4 2,370,113 + Psest_2220 0.56 -0.2 2,370,114 - Psest_2220 0.56 -0.0 2,370,114 - Psest_2220 0.56 +0.7 2,370,114 - Psest_2220 0.56 +3.4 2,370,114 - Psest_2220 0.56 -0.0 2,370,114 - Psest_2220 0.56 +0.1 2,370,114 - Psest_2220 0.56 +0.3 2,370,114 - Psest_2220 0.56 -0.2
Or see this region's nucleotide sequence