Experiment: Def Nalidixic 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2061 and Psest_2062 are separated by 280 nucleotides Psest_2062 and Psest_2063 are separated by 63 nucleotides Psest_2063 and Psest_2064 overlap by 20 nucleotides
Psest_2061: Psest_2061 - Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain, at 2,186,864 to 2,187,700
_2061
Psest_2062: Psest_2062 - Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases), at 2,187,981 to 2,188,745
_2062
Psest_2063: Psest_2063 - Enoyl-[acyl-carrier-protein] reductase (NADH), at 2,188,809 to 2,189,603
_2063
Psest_2064: Psest_2064 - ABC-type uncharacterized transport system, duplicated ATPase component, at 2,189,584 to 2,191,245
_2064
Position (kb)
2187
2188
2189 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2187.027 kb on + strand, within Psest_2061 at 2187.027 kb on + strand, within Psest_2061 at 2187.028 kb on - strand, within Psest_2061 at 2187.028 kb on - strand, within Psest_2061 at 2187.028 kb on - strand, within Psest_2061 at 2187.172 kb on - strand, within Psest_2061 at 2187.172 kb on - strand, within Psest_2061 at 2187.172 kb on - strand, within Psest_2061 at 2187.172 kb on - strand, within Psest_2061 at 2187.433 kb on - strand, within Psest_2061 at 2187.486 kb on + strand, within Psest_2061 at 2187.754 kb on + strand at 2187.754 kb on + strand at 2187.754 kb on + strand at 2187.755 kb on - strand at 2187.824 kb on - strand at 2188.172 kb on + strand, within Psest_2062 at 2188.342 kb on + strand, within Psest_2062 at 2188.342 kb on + strand, within Psest_2062 at 2188.343 kb on - strand, within Psest_2062 at 2188.343 kb on - strand, within Psest_2062 at 2188.343 kb on - strand, within Psest_2062 at 2188.410 kb on + strand, within Psest_2062 at 2188.420 kb on - strand, within Psest_2062 at 2188.420 kb on - strand, within Psest_2062 at 2188.447 kb on - strand, within Psest_2062 at 2188.790 kb on + strand at 2188.791 kb on - strand at 2188.794 kb on + strand at 2189.674 kb on - strand at 2189.674 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Nalidixic 0.001 mg/ml remove 2,187,027 + Psest_2061 0.19 -1.1 2,187,027 + Psest_2061 0.19 -0.4 2,187,028 - Psest_2061 0.20 +1.7 2,187,028 - Psest_2061 0.20 +0.3 2,187,028 - Psest_2061 0.20 +0.2 2,187,172 - Psest_2061 0.37 -1.1 2,187,172 - Psest_2061 0.37 +1.2 2,187,172 - Psest_2061 0.37 -2.4 2,187,172 - Psest_2061 0.37 +0.4 2,187,433 - Psest_2061 0.68 +0.1 2,187,486 + Psest_2061 0.74 -0.1 2,187,754 + +0.0 2,187,754 + +0.0 2,187,754 + -0.9 2,187,755 - -0.2 2,187,824 - -0.2 2,188,172 + Psest_2062 0.25 +0.5 2,188,342 + Psest_2062 0.47 -1.4 2,188,342 + Psest_2062 0.47 -1.2 2,188,343 - Psest_2062 0.47 -2.1 2,188,343 - Psest_2062 0.47 +0.2 2,188,343 - Psest_2062 0.47 +0.4 2,188,410 + Psest_2062 0.56 +0.8 2,188,420 - Psest_2062 0.57 +0.4 2,188,420 - Psest_2062 0.57 -1.2 2,188,447 - Psest_2062 0.61 -0.2 2,188,790 + +0.3 2,188,791 - +2.0 2,188,794 + -1.5 2,189,674 - +0.4 2,189,674 - -0.2
Or see this region's nucleotide sequence