Experiment: Def Nalidixic 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1995 and Psest_1996 are separated by 214 nucleotides Psest_1996 and Psest_1997 overlap by 14 nucleotides Psest_1997 and Psest_1998 are separated by 17 nucleotides
Psest_1995: Psest_1995 - protein-(glutamine-N5) methyltransferase, ribosomal protein L3-specific, at 2,112,086 to 2,113,009
_1995
Psest_1996: Psest_1996 - glucose-6-phosphate 1-dehydrogenase, at 2,113,224 to 2,114,693
_1996
Psest_1997: Psest_1997 - 6-phosphogluconolactonase, at 2,114,680 to 2,115,393
_1997
Psest_1998: Psest_1998 - Entner-Doudoroff aldolase, at 2,115,411 to 2,116,055
_1998
Position (kb)
2113
2114
2115 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2112.257 kb on + strand, within Psest_1995 at 2112.258 kb on - strand, within Psest_1995 at 2112.258 kb on - strand, within Psest_1995 at 2112.309 kb on + strand, within Psest_1995 at 2112.344 kb on + strand, within Psest_1995 at 2112.345 kb on - strand, within Psest_1995 at 2112.345 kb on - strand, within Psest_1995 at 2112.345 kb on - strand, within Psest_1995 at 2112.348 kb on + strand, within Psest_1995 at 2112.348 kb on + strand, within Psest_1995 at 2112.348 kb on + strand, within Psest_1995 at 2112.348 kb on + strand, within Psest_1995 at 2112.348 kb on + strand, within Psest_1995 at 2112.348 kb on + strand, within Psest_1995 at 2112.348 kb on + strand, within Psest_1995 at 2112.348 kb on + strand, within Psest_1995 at 2112.349 kb on - strand, within Psest_1995 at 2112.349 kb on - strand, within Psest_1995 at 2112.349 kb on - strand, within Psest_1995 at 2112.349 kb on - strand, within Psest_1995 at 2112.349 kb on - strand, within Psest_1995 at 2112.349 kb on - strand, within Psest_1995 at 2112.349 kb on - strand, within Psest_1995 at 2112.349 kb on - strand, within Psest_1995 at 2112.352 kb on - strand, within Psest_1995 at 2112.431 kb on - strand, within Psest_1995 at 2112.524 kb on + strand, within Psest_1995 at 2112.525 kb on - strand, within Psest_1995 at 2112.562 kb on - strand, within Psest_1995 at 2112.562 kb on - strand, within Psest_1995 at 2112.626 kb on + strand, within Psest_1995 at 2112.626 kb on + strand, within Psest_1995 at 2112.626 kb on + strand, within Psest_1995 at 2112.627 kb on - strand, within Psest_1995 at 2112.698 kb on + strand, within Psest_1995 at 2112.698 kb on + strand, within Psest_1995 at 2112.698 kb on + strand, within Psest_1995 at 2112.698 kb on + strand, within Psest_1995 at 2112.724 kb on + strand, within Psest_1995 at 2113.028 kb on + strand at 2113.029 kb on - strand at 2113.047 kb on + strand at 2113.048 kb on - strand at 2113.081 kb on + strand at 2113.081 kb on + strand at 2113.134 kb on + strand at 2113.134 kb on + strand at 2113.312 kb on - strand at 2113.485 kb on + strand, within Psest_1996 at 2113.485 kb on + strand, within Psest_1996 at 2113.518 kb on + strand, within Psest_1996 at 2113.518 kb on + strand, within Psest_1996 at 2113.519 kb on - strand, within Psest_1996 at 2113.519 kb on - strand, within Psest_1996 at 2113.522 kb on + strand, within Psest_1996 at 2113.566 kb on + strand, within Psest_1996 at 2113.566 kb on + strand, within Psest_1996 at 2113.588 kb on + strand, within Psest_1996 at 2113.588 kb on + strand, within Psest_1996 at 2113.588 kb on + strand, within Psest_1996 at 2113.588 kb on + strand, within Psest_1996 at 2113.588 kb on + strand, within Psest_1996 at 2113.588 kb on + strand, within Psest_1996 at 2113.588 kb on + strand, within Psest_1996 at 2113.588 kb on + strand, within Psest_1996 at 2113.588 kb on + strand, within Psest_1996 at 2113.588 kb on + strand, within Psest_1996 at 2113.588 kb on + strand, within Psest_1996 at 2113.588 kb on + strand, within Psest_1996 at 2113.588 kb on + strand, within Psest_1996 at 2113.588 kb on + strand, within Psest_1996 at 2113.588 kb on + strand, within Psest_1996 at 2113.588 kb on + strand, within Psest_1996 at 2113.588 kb on + strand, within Psest_1996 at 2113.589 kb on - strand, within Psest_1996 at 2113.589 kb on - strand, within Psest_1996 at 2113.589 kb on - strand, within Psest_1996 at 2113.589 kb on - strand, within Psest_1996 at 2113.589 kb on - strand, within Psest_1996 at 2113.589 kb on - strand, within Psest_1996 at 2113.589 kb on - strand, within Psest_1996 at 2113.589 kb on - strand, within Psest_1996 at 2113.589 kb on - strand, within Psest_1996 at 2113.589 kb on - strand, within Psest_1996 at 2113.589 kb on - strand, within Psest_1996 at 2113.589 kb on - strand, within Psest_1996 at 2113.589 kb on - strand, within Psest_1996 at 2113.589 kb on - strand, within Psest_1996 at 2113.591 kb on - strand, within Psest_1996 at 2113.591 kb on - strand, within Psest_1996 at 2113.734 kb on + strand, within Psest_1996 at 2113.750 kb on - strand, within Psest_1996 at 2113.750 kb on - strand, within Psest_1996 at 2113.750 kb on - strand, within Psest_1996 at 2113.753 kb on + strand, within Psest_1996 at 2113.753 kb on + strand, within Psest_1996 at 2113.753 kb on + strand, within Psest_1996 at 2113.753 kb on + strand, within Psest_1996 at 2113.753 kb on + strand, within Psest_1996 at 2113.753 kb on + strand, within Psest_1996 at 2113.753 kb on + strand, within Psest_1996 at 2113.753 kb on + strand, within Psest_1996 at 2113.753 kb on + strand, within Psest_1996 at 2113.754 kb on - strand, within Psest_1996 at 2113.754 kb on - strand, within Psest_1996 at 2113.754 kb on - strand, within Psest_1996 at 2113.754 kb on - strand, within Psest_1996 at 2113.890 kb on + strand, within Psest_1996 at 2113.992 kb on - strand, within Psest_1996 at 2114.018 kb on - strand, within Psest_1996 at 2114.079 kb on + strand, within Psest_1996 at 2114.189 kb on + strand, within Psest_1996 at 2114.189 kb on + strand, within Psest_1996 at 2114.217 kb on + strand, within Psest_1996 at 2114.218 kb on - strand, within Psest_1996 at 2114.218 kb on - strand, within Psest_1996 at 2114.218 kb on - strand, within Psest_1996 at 2114.218 kb on - strand, within Psest_1996 at 2114.289 kb on + strand, within Psest_1996 at 2114.289 kb on + strand, within Psest_1996 at 2114.290 kb on - strand, within Psest_1996 at 2114.360 kb on + strand, within Psest_1996 at 2114.360 kb on + strand, within Psest_1996 at 2114.361 kb on - strand, within Psest_1996 at 2114.361 kb on - strand, within Psest_1996 at 2114.361 kb on - strand, within Psest_1996 at 2114.406 kb on - strand, within Psest_1996 at 2114.451 kb on + strand, within Psest_1996 at 2114.451 kb on + strand, within Psest_1996 at 2114.452 kb on - strand, within Psest_1996 at 2114.478 kb on + strand, within Psest_1996 at 2114.491 kb on + strand, within Psest_1996 at 2114.491 kb on + strand, within Psest_1996 at 2114.491 kb on + strand, within Psest_1996 at 2114.492 kb on - strand, within Psest_1996 at 2114.532 kb on - strand, within Psest_1996 at 2114.975 kb on + strand, within Psest_1997 at 2115.241 kb on + strand, within Psest_1997 at 2115.252 kb on - strand, within Psest_1997 at 2115.361 kb on + strand at 2115.362 kb on - strand at 2115.362 kb on - strand at 2115.379 kb on + strand at 2115.485 kb on - strand, within Psest_1998
Per-strain Table
Position Strand Gene LocusTag Fraction Def Nalidixic 0.001 mg/ml remove 2,112,257 + Psest_1995 0.19 +0.3 2,112,258 - Psest_1995 0.19 +1.1 2,112,258 - Psest_1995 0.19 -0.2 2,112,309 + Psest_1995 0.24 -2.8 2,112,344 + Psest_1995 0.28 +0.0 2,112,345 - Psest_1995 0.28 +0.3 2,112,345 - Psest_1995 0.28 +2.4 2,112,345 - Psest_1995 0.28 +2.3 2,112,348 + Psest_1995 0.28 +1.1 2,112,348 + Psest_1995 0.28 -0.6 2,112,348 + Psest_1995 0.28 -0.1 2,112,348 + Psest_1995 0.28 +1.2 2,112,348 + Psest_1995 0.28 -0.0 2,112,348 + Psest_1995 0.28 +2.1 2,112,348 + Psest_1995 0.28 +1.3 2,112,348 + Psest_1995 0.28 -0.2 2,112,349 - Psest_1995 0.28 -0.7 2,112,349 - Psest_1995 0.28 +0.4 2,112,349 - Psest_1995 0.28 +0.5 2,112,349 - Psest_1995 0.28 +1.1 2,112,349 - Psest_1995 0.28 -0.2 2,112,349 - Psest_1995 0.28 +0.4 2,112,349 - Psest_1995 0.28 +1.8 2,112,349 - Psest_1995 0.28 +0.6 2,112,352 - Psest_1995 0.29 -0.8 2,112,431 - Psest_1995 0.37 -0.2 2,112,524 + Psest_1995 0.47 -0.3 2,112,525 - Psest_1995 0.48 +0.1 2,112,562 - Psest_1995 0.52 -0.5 2,112,562 - Psest_1995 0.52 +0.9 2,112,626 + Psest_1995 0.58 +1.9 2,112,626 + Psest_1995 0.58 +0.5 2,112,626 + Psest_1995 0.58 +0.2 2,112,627 - Psest_1995 0.59 -0.3 2,112,698 + Psest_1995 0.66 +1.0 2,112,698 + Psest_1995 0.66 +0.8 2,112,698 + Psest_1995 0.66 +1.8 2,112,698 + Psest_1995 0.66 +0.5 2,112,724 + Psest_1995 0.69 -0.9 2,113,028 + -1.7 2,113,029 - -0.8 2,113,047 + -0.3 2,113,048 - -1.4 2,113,081 + +0.4 2,113,081 + +0.7 2,113,134 + -0.1 2,113,134 + -0.8 2,113,312 - -0.3 2,113,485 + Psest_1996 0.18 +0.8 2,113,485 + Psest_1996 0.18 -0.6 2,113,518 + Psest_1996 0.20 +0.1 2,113,518 + Psest_1996 0.20 +1.1 2,113,519 - Psest_1996 0.20 +0.1 2,113,519 - Psest_1996 0.20 -0.2 2,113,522 + Psest_1996 0.20 -0.5 2,113,566 + Psest_1996 0.23 -0.7 2,113,566 + Psest_1996 0.23 +0.2 2,113,588 + Psest_1996 0.25 +0.1 2,113,588 + Psest_1996 0.25 +0.0 2,113,588 + Psest_1996 0.25 -0.9 2,113,588 + Psest_1996 0.25 +1.2 2,113,588 + Psest_1996 0.25 +0.1 2,113,588 + Psest_1996 0.25 -0.2 2,113,588 + Psest_1996 0.25 +0.7 2,113,588 + Psest_1996 0.25 +0.1 2,113,588 + Psest_1996 0.25 -0.4 2,113,588 + Psest_1996 0.25 -0.5 2,113,588 + Psest_1996 0.25 +2.1 2,113,588 + Psest_1996 0.25 -0.9 2,113,588 + Psest_1996 0.25 -0.2 2,113,588 + Psest_1996 0.25 -1.2 2,113,588 + Psest_1996 0.25 -1.2 2,113,588 + Psest_1996 0.25 +1.2 2,113,588 + Psest_1996 0.25 -1.3 2,113,589 - Psest_1996 0.25 -1.0 2,113,589 - Psest_1996 0.25 -0.5 2,113,589 - Psest_1996 0.25 -0.2 2,113,589 - Psest_1996 0.25 -0.5 2,113,589 - Psest_1996 0.25 -0.4 2,113,589 - Psest_1996 0.25 -1.4 2,113,589 - Psest_1996 0.25 -0.5 2,113,589 - Psest_1996 0.25 +0.6 2,113,589 - Psest_1996 0.25 -0.1 2,113,589 - Psest_1996 0.25 -1.8 2,113,589 - Psest_1996 0.25 +0.5 2,113,589 - Psest_1996 0.25 +0.2 2,113,589 - Psest_1996 0.25 -0.1 2,113,589 - Psest_1996 0.25 +0.6 2,113,591 - Psest_1996 0.25 +1.1 2,113,591 - Psest_1996 0.25 +0.3 2,113,734 + Psest_1996 0.35 +0.1 2,113,750 - Psest_1996 0.36 +0.6 2,113,750 - Psest_1996 0.36 +0.4 2,113,750 - Psest_1996 0.36 +0.5 2,113,753 + Psest_1996 0.36 -0.9 2,113,753 + Psest_1996 0.36 -0.4 2,113,753 + Psest_1996 0.36 -0.2 2,113,753 + Psest_1996 0.36 -0.4 2,113,753 + Psest_1996 0.36 -1.5 2,113,753 + Psest_1996 0.36 -0.2 2,113,753 + Psest_1996 0.36 -0.2 2,113,753 + Psest_1996 0.36 -0.3 2,113,753 + Psest_1996 0.36 -0.0 2,113,754 - Psest_1996 0.36 -0.6 2,113,754 - Psest_1996 0.36 -0.1 2,113,754 - Psest_1996 0.36 -1.2 2,113,754 - Psest_1996 0.36 +0.8 2,113,890 + Psest_1996 0.45 +0.3 2,113,992 - Psest_1996 0.52 +0.3 2,114,018 - Psest_1996 0.54 -0.2 2,114,079 + Psest_1996 0.58 -1.4 2,114,189 + Psest_1996 0.66 -0.1 2,114,189 + Psest_1996 0.66 +0.1 2,114,217 + Psest_1996 0.68 -1.2 2,114,218 - Psest_1996 0.68 -0.6 2,114,218 - Psest_1996 0.68 -0.0 2,114,218 - Psest_1996 0.68 -0.3 2,114,218 - Psest_1996 0.68 -0.0 2,114,289 + Psest_1996 0.72 +0.1 2,114,289 + Psest_1996 0.72 -0.2 2,114,290 - Psest_1996 0.73 +0.8 2,114,360 + Psest_1996 0.77 -0.9 2,114,360 + Psest_1996 0.77 -0.6 2,114,361 - Psest_1996 0.77 +0.8 2,114,361 - Psest_1996 0.77 -0.2 2,114,361 - Psest_1996 0.77 -0.3 2,114,406 - Psest_1996 0.80 +0.3 2,114,451 + Psest_1996 0.83 +0.2 2,114,451 + Psest_1996 0.83 +0.5 2,114,452 - Psest_1996 0.84 -0.5 2,114,478 + Psest_1996 0.85 -1.0 2,114,491 + Psest_1996 0.86 -0.5 2,114,491 + Psest_1996 0.86 +0.7 2,114,491 + Psest_1996 0.86 +0.0 2,114,492 - Psest_1996 0.86 -1.1 2,114,532 - Psest_1996 0.89 -0.3 2,114,975 + Psest_1997 0.41 +0.3 2,115,241 + Psest_1997 0.79 -1.1 2,115,252 - Psest_1997 0.80 +0.4 2,115,361 + +0.3 2,115,362 - -0.3 2,115,362 - +0.8 2,115,379 + +0.9 2,115,485 - Psest_1998 0.11 -0.7
Or see this region's nucleotide sequence