Experiment: Def Nalidixic 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0157 and Psest_0158 are separated by 230 nucleotides Psest_0158 and Psest_0159 are separated by 3 nucleotides Psest_0159 and Psest_0160 are separated by 2 nucleotides
Psest_0157: Psest_0157 - ABC-type amino acid transport/signal transduction systems, periplasmic component/domain, at 155,327 to 156,076
_0157
Psest_0158: Psest_0158 - ABC-type amino acid transport/signal transduction systems, periplasmic component/domain, at 156,307 to 157,053
_0158
Psest_0159: Psest_0159 - Uncharacterized protein conserved in bacteria, at 157,057 to 157,848
_0159
Psest_0160: Psest_0160 - C-terminal peptidase (prc), at 157,851 to 159,170
_0160
Position (kb) 156
157
158 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 155.368 kb on - strand at 155.438 kb on + strand, within Psest_0157 at 155.439 kb on - strand, within Psest_0157 at 155.540 kb on + strand, within Psest_0157 at 155.540 kb on + strand, within Psest_0157 at 155.576 kb on + strand, within Psest_0157 at 155.576 kb on + strand, within Psest_0157 at 155.576 kb on + strand, within Psest_0157 at 155.577 kb on - strand, within Psest_0157 at 155.603 kb on + strand, within Psest_0157 at 155.603 kb on + strand, within Psest_0157 at 155.603 kb on + strand, within Psest_0157 at 155.603 kb on + strand, within Psest_0157 at 155.604 kb on - strand, within Psest_0157 at 155.604 kb on - strand, within Psest_0157 at 155.744 kb on + strand, within Psest_0157 at 155.744 kb on + strand, within Psest_0157 at 155.744 kb on + strand, within Psest_0157 at 155.744 kb on + strand, within Psest_0157 at 155.744 kb on + strand, within Psest_0157 at 155.744 kb on + strand, within Psest_0157 at 155.745 kb on - strand, within Psest_0157 at 155.745 kb on - strand, within Psest_0157 at 155.745 kb on - strand, within Psest_0157 at 155.759 kb on + strand, within Psest_0157 at 155.759 kb on + strand, within Psest_0157 at 155.760 kb on - strand, within Psest_0157 at 155.760 kb on - strand, within Psest_0157 at 155.760 kb on - strand, within Psest_0157 at 155.760 kb on - strand, within Psest_0157 at 155.951 kb on - strand, within Psest_0157 at 155.963 kb on + strand, within Psest_0157 at 155.964 kb on - strand, within Psest_0157 at 156.069 kb on - strand at 156.069 kb on - strand at 156.323 kb on + strand at 156.344 kb on + strand at 156.345 kb on - strand at 156.345 kb on - strand at 156.345 kb on - strand at 156.442 kb on + strand, within Psest_0158 at 156.443 kb on - strand, within Psest_0158 at 156.443 kb on - strand, within Psest_0158 at 156.443 kb on - strand, within Psest_0158 at 156.443 kb on - strand, within Psest_0158 at 156.553 kb on + strand, within Psest_0158 at 156.554 kb on - strand, within Psest_0158 at 156.554 kb on - strand, within Psest_0158 at 156.554 kb on - strand, within Psest_0158 at 156.652 kb on - strand, within Psest_0158 at 156.736 kb on + strand, within Psest_0158 at 156.736 kb on + strand, within Psest_0158 at 156.874 kb on + strand, within Psest_0158 at 156.987 kb on - strand at 157.059 kb on - strand at 157.065 kb on - strand at 157.435 kb on + strand, within Psest_0159 at 157.435 kb on + strand, within Psest_0159 at 157.436 kb on - strand, within Psest_0159 at 157.436 kb on - strand, within Psest_0159 at 157.537 kb on + strand, within Psest_0159 at 157.537 kb on + strand, within Psest_0159 at 157.732 kb on + strand, within Psest_0159 at 157.790 kb on + strand at 157.791 kb on - strand at 157.792 kb on + strand at 157.793 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Nalidixic 0.001 mg/ml remove 155,368 - -2.0 155,438 + Psest_0157 0.15 +0.1 155,439 - Psest_0157 0.15 -1.7 155,540 + Psest_0157 0.28 +0.5 155,540 + Psest_0157 0.28 +1.3 155,576 + Psest_0157 0.33 +0.7 155,576 + Psest_0157 0.33 -0.4 155,576 + Psest_0157 0.33 -1.7 155,577 - Psest_0157 0.33 -1.0 155,603 + Psest_0157 0.37 +0.6 155,603 + Psest_0157 0.37 -0.6 155,603 + Psest_0157 0.37 -0.3 155,603 + Psest_0157 0.37 -0.8 155,604 - Psest_0157 0.37 -3.4 155,604 - Psest_0157 0.37 -1.1 155,744 + Psest_0157 0.56 -1.1 155,744 + Psest_0157 0.56 +0.5 155,744 + Psest_0157 0.56 +0.3 155,744 + Psest_0157 0.56 -1.2 155,744 + Psest_0157 0.56 -0.7 155,744 + Psest_0157 0.56 +0.7 155,745 - Psest_0157 0.56 -2.6 155,745 - Psest_0157 0.56 -0.9 155,745 - Psest_0157 0.56 +0.1 155,759 + Psest_0157 0.58 -0.3 155,759 + Psest_0157 0.58 +0.2 155,760 - Psest_0157 0.58 -0.8 155,760 - Psest_0157 0.58 -1.2 155,760 - Psest_0157 0.58 -1.7 155,760 - Psest_0157 0.58 -1.3 155,951 - Psest_0157 0.83 +0.1 155,963 + Psest_0157 0.85 +0.1 155,964 - Psest_0157 0.85 -1.1 156,069 - -0.9 156,069 - -0.6 156,323 + -1.3 156,344 + -0.9 156,345 - -1.5 156,345 - -0.9 156,345 - -0.7 156,442 + Psest_0158 0.18 -0.6 156,443 - Psest_0158 0.18 -3.6 156,443 - Psest_0158 0.18 -0.8 156,443 - Psest_0158 0.18 -2.4 156,443 - Psest_0158 0.18 -1.0 156,553 + Psest_0158 0.33 -1.1 156,554 - Psest_0158 0.33 -0.8 156,554 - Psest_0158 0.33 -1.5 156,554 - Psest_0158 0.33 -1.1 156,652 - Psest_0158 0.46 -1.3 156,736 + Psest_0158 0.57 +0.7 156,736 + Psest_0158 0.57 -1.3 156,874 + Psest_0158 0.76 +0.3 156,987 - -1.3 157,059 - -0.6 157,065 - -0.6 157,435 + Psest_0159 0.48 +0.6 157,435 + Psest_0159 0.48 -0.3 157,436 - Psest_0159 0.48 +0.1 157,436 - Psest_0159 0.48 -0.0 157,537 + Psest_0159 0.61 +0.2 157,537 + Psest_0159 0.61 -3.1 157,732 + Psest_0159 0.85 +1.0 157,790 + -0.1 157,791 - -0.8 157,792 + -0.1 157,793 - -1.5
Or see this region's nucleotide sequence