Experiment: Def Nalidixic 0.0005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2437 and Psest_2438 are separated by 36 nucleotides Psest_2438 and Psest_2439 are separated by 2 nucleotides Psest_2439 and Psest_2440 are separated by 38 nucleotides
Psest_2437: Psest_2437 - Enoyl-CoA hydratase [valine degradation] (EC 4.2.1.17) (from data) , at 2,605,492 to 2,606,265
_2437
Psest_2438: Psest_2438 - 3-hydroxyisobutyrate dehydrogenase (EC 1.1.1.31) (from data) , at 2,606,302 to 2,607,192
_2438
Psest_2439: Psest_2439 - 3-hydroxyisobutyryl-CoA hydrolase (EC 3.1.2.4) (from data) , at 2,607,195 to 2,608,289
_2439
Psest_2440: Psest_2440 - isobutyryl-CoA dehydrogenase (EC 1.3.8.5) (from data) , at 2,608,328 to 2,609,479
_2440
Position (kb)
2607
2608
2609 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2606.280 kb on + strand at 2606.316 kb on + strand at 2606.317 kb on - strand at 2606.524 kb on + strand, within Psest_2438 at 2606.524 kb on + strand, within Psest_2438 at 2606.524 kb on + strand, within Psest_2438 at 2606.956 kb on + strand, within Psest_2438 at 2606.956 kb on + strand, within Psest_2438 at 2606.956 kb on + strand, within Psest_2438 at 2606.956 kb on + strand, within Psest_2438 at 2606.956 kb on + strand, within Psest_2438 at 2606.956 kb on + strand, within Psest_2438 at 2606.956 kb on + strand, within Psest_2438 at 2606.957 kb on - strand, within Psest_2438 at 2606.957 kb on - strand, within Psest_2438 at 2606.957 kb on - strand, within Psest_2438 at 2606.957 kb on - strand, within Psest_2438 at 2606.957 kb on - strand, within Psest_2438 at 2606.957 kb on - strand, within Psest_2438 at 2606.957 kb on - strand, within Psest_2438 at 2606.957 kb on - strand, within Psest_2438 at 2606.957 kb on - strand, within Psest_2438 at 2606.957 kb on - strand, within Psest_2438 at 2606.957 kb on - strand, within Psest_2438 at 2606.957 kb on - strand, within Psest_2438 at 2606.957 kb on - strand, within Psest_2438 at 2606.957 kb on - strand, within Psest_2438 at 2606.957 kb on - strand, within Psest_2438 at 2607.004 kb on + strand, within Psest_2438 at 2607.230 kb on - strand at 2607.468 kb on + strand, within Psest_2439 at 2607.469 kb on - strand, within Psest_2439 at 2607.483 kb on + strand, within Psest_2439 at 2607.483 kb on + strand, within Psest_2439 at 2607.484 kb on - strand, within Psest_2439 at 2607.602 kb on + strand, within Psest_2439 at 2607.625 kb on - strand, within Psest_2439 at 2607.648 kb on + strand, within Psest_2439 at 2607.648 kb on + strand, within Psest_2439 at 2607.648 kb on + strand, within Psest_2439 at 2607.649 kb on - strand, within Psest_2439 at 2607.684 kb on + strand, within Psest_2439 at 2607.684 kb on + strand, within Psest_2439 at 2607.684 kb on + strand, within Psest_2439 at 2607.684 kb on + strand, within Psest_2439 at 2607.684 kb on + strand, within Psest_2439 at 2607.684 kb on + strand, within Psest_2439 at 2607.684 kb on + strand, within Psest_2439 at 2607.684 kb on + strand, within Psest_2439 at 2607.685 kb on - strand, within Psest_2439 at 2607.685 kb on - strand, within Psest_2439 at 2607.685 kb on - strand, within Psest_2439 at 2607.685 kb on - strand, within Psest_2439 at 2607.685 kb on - strand, within Psest_2439 at 2607.685 kb on - strand, within Psest_2439 at 2607.685 kb on - strand, within Psest_2439 at 2607.685 kb on - strand, within Psest_2439 at 2607.685 kb on - strand, within Psest_2439 at 2607.685 kb on - strand, within Psest_2439 at 2607.685 kb on - strand, within Psest_2439 at 2607.685 kb on - strand, within Psest_2439 at 2607.685 kb on - strand, within Psest_2439 at 2607.685 kb on - strand, within Psest_2439 at 2608.517 kb on + strand, within Psest_2440 at 2608.517 kb on + strand, within Psest_2440 at 2608.517 kb on + strand, within Psest_2440 at 2608.517 kb on + strand, within Psest_2440 at 2608.517 kb on + strand, within Psest_2440 at 2608.517 kb on + strand, within Psest_2440 at 2608.517 kb on + strand, within Psest_2440 at 2608.517 kb on + strand, within Psest_2440 at 2608.517 kb on + strand, within Psest_2440 at 2608.518 kb on - strand, within Psest_2440 at 2608.518 kb on - strand, within Psest_2440 at 2608.518 kb on - strand, within Psest_2440 at 2608.518 kb on - strand, within Psest_2440 at 2608.518 kb on - strand, within Psest_2440 at 2608.518 kb on - strand, within Psest_2440 at 2608.520 kb on - strand, within Psest_2440 at 2608.526 kb on + strand, within Psest_2440 at 2608.527 kb on - strand, within Psest_2440 at 2608.527 kb on - strand, within Psest_2440 at 2608.632 kb on - strand, within Psest_2440 at 2609.120 kb on + strand, within Psest_2440
Per-strain Table
Position Strand Gene LocusTag Fraction Def Nalidixic 0.0005 mg/ml remove 2,606,280 + +0.0 2,606,316 + +0.0 2,606,317 - -1.8 2,606,524 + Psest_2438 0.25 -1.0 2,606,524 + Psest_2438 0.25 +0.2 2,606,524 + Psest_2438 0.25 +0.7 2,606,956 + Psest_2438 0.73 -1.0 2,606,956 + Psest_2438 0.73 -0.8 2,606,956 + Psest_2438 0.73 -0.5 2,606,956 + Psest_2438 0.73 -0.3 2,606,956 + Psest_2438 0.73 +1.6 2,606,956 + Psest_2438 0.73 -0.5 2,606,956 + Psest_2438 0.73 +0.5 2,606,957 - Psest_2438 0.74 -0.2 2,606,957 - Psest_2438 0.74 +0.3 2,606,957 - Psest_2438 0.74 +0.8 2,606,957 - Psest_2438 0.74 -0.7 2,606,957 - Psest_2438 0.74 -0.5 2,606,957 - Psest_2438 0.74 -0.4 2,606,957 - Psest_2438 0.74 +0.9 2,606,957 - Psest_2438 0.74 +0.7 2,606,957 - Psest_2438 0.74 +0.4 2,606,957 - Psest_2438 0.74 +0.6 2,606,957 - Psest_2438 0.74 +0.9 2,606,957 - Psest_2438 0.74 +0.2 2,606,957 - Psest_2438 0.74 -0.2 2,606,957 - Psest_2438 0.74 -1.0 2,606,957 - Psest_2438 0.74 -0.4 2,607,004 + Psest_2438 0.79 +0.5 2,607,230 - +0.4 2,607,468 + Psest_2439 0.25 -2.4 2,607,469 - Psest_2439 0.25 +0.4 2,607,483 + Psest_2439 0.26 -0.4 2,607,483 + Psest_2439 0.26 +2.6 2,607,484 - Psest_2439 0.26 -0.0 2,607,602 + Psest_2439 0.37 +0.0 2,607,625 - Psest_2439 0.39 +0.4 2,607,648 + Psest_2439 0.41 -0.6 2,607,648 + Psest_2439 0.41 -0.4 2,607,648 + Psest_2439 0.41 -2.0 2,607,649 - Psest_2439 0.41 +0.8 2,607,684 + Psest_2439 0.45 -1.0 2,607,684 + Psest_2439 0.45 +0.0 2,607,684 + Psest_2439 0.45 -0.5 2,607,684 + Psest_2439 0.45 -0.0 2,607,684 + Psest_2439 0.45 +0.9 2,607,684 + Psest_2439 0.45 -0.2 2,607,684 + Psest_2439 0.45 -0.7 2,607,684 + Psest_2439 0.45 +0.3 2,607,685 - Psest_2439 0.45 +1.0 2,607,685 - Psest_2439 0.45 -0.6 2,607,685 - Psest_2439 0.45 -1.2 2,607,685 - Psest_2439 0.45 +0.8 2,607,685 - Psest_2439 0.45 -0.4 2,607,685 - Psest_2439 0.45 -0.4 2,607,685 - Psest_2439 0.45 -0.5 2,607,685 - Psest_2439 0.45 +0.3 2,607,685 - Psest_2439 0.45 +0.1 2,607,685 - Psest_2439 0.45 -0.6 2,607,685 - Psest_2439 0.45 +0.6 2,607,685 - Psest_2439 0.45 +0.5 2,607,685 - Psest_2439 0.45 +0.0 2,607,685 - Psest_2439 0.45 +0.1 2,608,517 + Psest_2440 0.16 -1.2 2,608,517 + Psest_2440 0.16 +1.0 2,608,517 + Psest_2440 0.16 +0.6 2,608,517 + Psest_2440 0.16 +0.4 2,608,517 + Psest_2440 0.16 -0.3 2,608,517 + Psest_2440 0.16 -0.1 2,608,517 + Psest_2440 0.16 -2.5 2,608,517 + Psest_2440 0.16 +0.3 2,608,517 + Psest_2440 0.16 -0.2 2,608,518 - Psest_2440 0.16 -0.5 2,608,518 - Psest_2440 0.16 -0.5 2,608,518 - Psest_2440 0.16 +1.5 2,608,518 - Psest_2440 0.16 +0.6 2,608,518 - Psest_2440 0.16 +1.0 2,608,518 - Psest_2440 0.16 +0.8 2,608,520 - Psest_2440 0.17 +0.4 2,608,526 + Psest_2440 0.17 -0.5 2,608,527 - Psest_2440 0.17 -1.0 2,608,527 - Psest_2440 0.17 -1.0 2,608,632 - Psest_2440 0.26 -1.1 2,609,120 + Psest_2440 0.69 -0.5
Or see this region's nucleotide sequence