Experiment: Def Nalidixic 0.0005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2436 and Psest_2437 are separated by 47 nucleotides Psest_2437 and Psest_2438 are separated by 36 nucleotides Psest_2438 and Psest_2439 are separated by 2 nucleotides
Psest_2436: Psest_2436 - Methylmalonate-semialdehyde dehydrogenase (EC 1.2.1.27) (from data) , at 2,603,933 to 2,605,444
_2436
Psest_2437: Psest_2437 - Enoyl-CoA hydratase [valine degradation] (EC 4.2.1.17) (from data) , at 2,605,492 to 2,606,265
_2437
Psest_2438: Psest_2438 - 3-hydroxyisobutyrate dehydrogenase (EC 1.1.1.31) (from data) , at 2,606,302 to 2,607,192
_2438
Psest_2439: Psest_2439 - 3-hydroxyisobutyryl-CoA hydrolase (EC 3.1.2.4) (from data) , at 2,607,195 to 2,608,289
_2439
Position (kb)
2605
2606
2607 Strain fitness (log2 ratio)
-2
-1
0
1 at 2604.651 kb on - strand, within Psest_2436 at 2604.651 kb on - strand, within Psest_2436 at 2605.083 kb on - strand, within Psest_2436 at 2605.260 kb on + strand, within Psest_2436 at 2605.358 kb on + strand at 2605.358 kb on + strand at 2605.359 kb on - strand at 2605.359 kb on - strand at 2605.359 kb on - strand at 2605.359 kb on - strand at 2605.542 kb on + strand at 2605.714 kb on + strand, within Psest_2437 at 2605.726 kb on + strand, within Psest_2437 at 2605.727 kb on - strand, within Psest_2437 at 2605.727 kb on - strand, within Psest_2437 at 2605.898 kb on + strand, within Psest_2437 at 2606.280 kb on + strand at 2606.316 kb on + strand at 2606.317 kb on - strand at 2606.524 kb on + strand, within Psest_2438 at 2606.524 kb on + strand, within Psest_2438 at 2606.524 kb on + strand, within Psest_2438 at 2606.956 kb on + strand, within Psest_2438 at 2606.956 kb on + strand, within Psest_2438 at 2606.956 kb on + strand, within Psest_2438 at 2606.956 kb on + strand, within Psest_2438 at 2606.956 kb on + strand, within Psest_2438 at 2606.956 kb on + strand, within Psest_2438 at 2606.956 kb on + strand, within Psest_2438 at 2606.957 kb on - strand, within Psest_2438 at 2606.957 kb on - strand, within Psest_2438 at 2606.957 kb on - strand, within Psest_2438 at 2606.957 kb on - strand, within Psest_2438 at 2606.957 kb on - strand, within Psest_2438 at 2606.957 kb on - strand, within Psest_2438 at 2606.957 kb on - strand, within Psest_2438 at 2606.957 kb on - strand, within Psest_2438 at 2606.957 kb on - strand, within Psest_2438 at 2606.957 kb on - strand, within Psest_2438 at 2606.957 kb on - strand, within Psest_2438 at 2606.957 kb on - strand, within Psest_2438 at 2606.957 kb on - strand, within Psest_2438 at 2606.957 kb on - strand, within Psest_2438 at 2606.957 kb on - strand, within Psest_2438 at 2607.004 kb on + strand, within Psest_2438 at 2607.230 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Nalidixic 0.0005 mg/ml remove 2,604,651 - Psest_2436 0.47 +1.0 2,604,651 - Psest_2436 0.47 +0.1 2,605,083 - Psest_2436 0.76 +0.0 2,605,260 + Psest_2436 0.88 +0.8 2,605,358 + +0.8 2,605,358 + -0.2 2,605,359 - -0.2 2,605,359 - +0.3 2,605,359 - +0.2 2,605,359 - +0.5 2,605,542 + +0.2 2,605,714 + Psest_2437 0.29 -0.1 2,605,726 + Psest_2437 0.30 -0.6 2,605,727 - Psest_2437 0.30 +0.4 2,605,727 - Psest_2437 0.30 -0.2 2,605,898 + Psest_2437 0.52 -0.2 2,606,280 + +0.0 2,606,316 + +0.0 2,606,317 - -1.8 2,606,524 + Psest_2438 0.25 -1.0 2,606,524 + Psest_2438 0.25 +0.2 2,606,524 + Psest_2438 0.25 +0.7 2,606,956 + Psest_2438 0.73 -1.0 2,606,956 + Psest_2438 0.73 -0.8 2,606,956 + Psest_2438 0.73 -0.5 2,606,956 + Psest_2438 0.73 -0.3 2,606,956 + Psest_2438 0.73 +1.6 2,606,956 + Psest_2438 0.73 -0.5 2,606,956 + Psest_2438 0.73 +0.5 2,606,957 - Psest_2438 0.74 -0.2 2,606,957 - Psest_2438 0.74 +0.3 2,606,957 - Psest_2438 0.74 +0.8 2,606,957 - Psest_2438 0.74 -0.7 2,606,957 - Psest_2438 0.74 -0.5 2,606,957 - Psest_2438 0.74 -0.4 2,606,957 - Psest_2438 0.74 +0.9 2,606,957 - Psest_2438 0.74 +0.7 2,606,957 - Psest_2438 0.74 +0.4 2,606,957 - Psest_2438 0.74 +0.6 2,606,957 - Psest_2438 0.74 +0.9 2,606,957 - Psest_2438 0.74 +0.2 2,606,957 - Psest_2438 0.74 -0.2 2,606,957 - Psest_2438 0.74 -1.0 2,606,957 - Psest_2438 0.74 -0.4 2,607,004 + Psest_2438 0.79 +0.5 2,607,230 - +0.4
Or see this region's nucleotide sequence