Experiment: Def Nalidixic 0.0005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2134 and Psest_2135 are separated by 87 nucleotides Psest_2135 and Psest_2136 are separated by 271 nucleotides Psest_2136 and Psest_2137 are separated by 110 nucleotides
Psest_2134: Psest_2134 - amino acid carrier protein, at 2,270,200 to 2,271,543
_2134
Psest_2135: Psest_2135 - Peroxiredoxin, at 2,271,631 to 2,272,131
_2135
Psest_2136: Psest_2136 - Predicted homoserine dehydrogenase, at 2,272,403 to 2,273,635
_2136
Psest_2137: Psest_2137 - Uncharacterized membrane protein, putative virulence factor, at 2,273,746 to 2,275,212
_2137
Position (kb)
2272
2273
2274 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2271.526 kb on + strand at 2271.527 kb on - strand at 2271.537 kb on + strand at 2271.537 kb on + strand at 2271.537 kb on + strand at 2271.538 kb on - strand at 2271.538 kb on - strand at 2271.538 kb on - strand at 2271.873 kb on + strand, within Psest_2135 at 2271.874 kb on - strand, within Psest_2135 at 2271.980 kb on - strand, within Psest_2135 at 2271.980 kb on - strand, within Psest_2135 at 2271.980 kb on - strand, within Psest_2135 at 2272.169 kb on + strand at 2272.265 kb on + strand at 2272.268 kb on + strand at 2272.268 kb on + strand at 2272.268 kb on + strand at 2272.268 kb on + strand at 2272.269 kb on - strand at 2272.269 kb on - strand at 2272.333 kb on - strand at 2272.373 kb on - strand at 2272.402 kb on + strand at 2272.402 kb on + strand at 2272.402 kb on + strand at 2272.403 kb on - strand at 2272.403 kb on - strand at 2272.403 kb on - strand at 2272.408 kb on + strand at 2272.408 kb on + strand at 2272.408 kb on + strand at 2272.721 kb on + strand, within Psest_2136 at 2272.722 kb on - strand, within Psest_2136 at 2272.834 kb on + strand, within Psest_2136 at 2272.835 kb on - strand, within Psest_2136 at 2273.090 kb on + strand, within Psest_2136 at 2273.090 kb on + strand, within Psest_2136 at 2273.171 kb on + strand, within Psest_2136 at 2273.171 kb on + strand, within Psest_2136 at 2273.171 kb on + strand, within Psest_2136 at 2273.172 kb on - strand, within Psest_2136 at 2273.172 kb on - strand, within Psest_2136 at 2273.181 kb on + strand, within Psest_2136 at 2273.181 kb on + strand, within Psest_2136 at 2273.182 kb on - strand, within Psest_2136 at 2273.199 kb on - strand, within Psest_2136 at 2273.199 kb on - strand, within Psest_2136 at 2273.287 kb on + strand, within Psest_2136 at 2273.424 kb on + strand, within Psest_2136 at 2273.425 kb on - strand, within Psest_2136 at 2273.615 kb on - strand at 2273.844 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Nalidixic 0.0005 mg/ml remove 2,271,526 + -0.4 2,271,527 - -1.6 2,271,537 + +1.0 2,271,537 + -2.5 2,271,537 + -0.4 2,271,538 - -1.3 2,271,538 - -0.0 2,271,538 - +1.0 2,271,873 + Psest_2135 0.48 +0.4 2,271,874 - Psest_2135 0.49 -0.5 2,271,980 - Psest_2135 0.70 -0.2 2,271,980 - Psest_2135 0.70 -0.3 2,271,980 - Psest_2135 0.70 -0.8 2,272,169 + -0.5 2,272,265 + -0.2 2,272,268 + -0.9 2,272,268 + -0.6 2,272,268 + -0.3 2,272,268 + -0.3 2,272,269 - -1.6 2,272,269 - -1.3 2,272,333 - -0.1 2,272,373 - -0.7 2,272,402 + -0.7 2,272,402 + -0.0 2,272,402 + -0.0 2,272,403 - -1.1 2,272,403 - -1.0 2,272,403 - -0.6 2,272,408 + -1.1 2,272,408 + -0.8 2,272,408 + -1.6 2,272,721 + Psest_2136 0.26 -1.2 2,272,722 - Psest_2136 0.26 +0.4 2,272,834 + Psest_2136 0.35 -1.3 2,272,835 - Psest_2136 0.35 -1.2 2,273,090 + Psest_2136 0.56 -1.3 2,273,090 + Psest_2136 0.56 -1.2 2,273,171 + Psest_2136 0.62 -0.8 2,273,171 + Psest_2136 0.62 -0.9 2,273,171 + Psest_2136 0.62 -0.7 2,273,172 - Psest_2136 0.62 -1.0 2,273,172 - Psest_2136 0.62 -2.0 2,273,181 + Psest_2136 0.63 -1.0 2,273,181 + Psest_2136 0.63 -1.4 2,273,182 - Psest_2136 0.63 -3.8 2,273,199 - Psest_2136 0.65 -1.2 2,273,199 - Psest_2136 0.65 -3.1 2,273,287 + Psest_2136 0.72 +0.2 2,273,424 + Psest_2136 0.83 +0.2 2,273,425 - Psest_2136 0.83 -1.4 2,273,615 - -0.6 2,273,844 - -0.6
Or see this region's nucleotide sequence