Experiment: Def Tetracycline 0.0004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0475 and Psest_0476 are separated by 196 nucleotides Psest_0476 and Psest_0477 are separated by 382 nucleotides
Psest_0475: Psest_0475 - 3-deoxy-D-manno-octulosonic-acid transferase, at 523,442 to 524,710
_0475
Psest_0476: Psest_0476 - type I secretion outer membrane protein, TolC family, at 524,907 to 526,361
_0476
Psest_0477: Psest_0477 - thiamine biosynthesis protein ThiC, at 526,744 to 528,624
_0477
Position (kb)
524
525
526
527 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 524.796 kb on + strand at 524.827 kb on + strand at 524.830 kb on + strand at 524.830 kb on + strand at 524.831 kb on - strand at 524.831 kb on - strand at 524.831 kb on - strand at 524.864 kb on + strand at 524.864 kb on + strand at 524.865 kb on - strand at 524.865 kb on - strand at 524.980 kb on + strand at 524.980 kb on + strand at 524.980 kb on + strand at 525.070 kb on + strand, within Psest_0476 at 525.070 kb on + strand, within Psest_0476 at 525.070 kb on + strand, within Psest_0476 at 525.071 kb on - strand, within Psest_0476 at 525.071 kb on - strand, within Psest_0476 at 525.071 kb on - strand, within Psest_0476 at 525.071 kb on - strand, within Psest_0476 at 525.071 kb on - strand, within Psest_0476 at 525.071 kb on - strand, within Psest_0476 at 525.091 kb on + strand, within Psest_0476 at 525.091 kb on + strand, within Psest_0476 at 525.091 kb on + strand, within Psest_0476 at 525.092 kb on - strand, within Psest_0476 at 525.092 kb on - strand, within Psest_0476 at 525.132 kb on + strand, within Psest_0476 at 525.132 kb on + strand, within Psest_0476 at 525.328 kb on + strand, within Psest_0476 at 525.331 kb on + strand, within Psest_0476 at 525.331 kb on + strand, within Psest_0476 at 525.332 kb on - strand, within Psest_0476 at 525.332 kb on - strand, within Psest_0476 at 525.370 kb on + strand, within Psest_0476 at 525.371 kb on - strand, within Psest_0476 at 525.371 kb on - strand, within Psest_0476 at 525.575 kb on - strand, within Psest_0476 at 525.691 kb on + strand, within Psest_0476 at 525.691 kb on + strand, within Psest_0476 at 525.691 kb on + strand, within Psest_0476 at 525.692 kb on - strand, within Psest_0476 at 525.692 kb on - strand, within Psest_0476 at 525.883 kb on - strand, within Psest_0476 at 525.922 kb on + strand, within Psest_0476 at 525.923 kb on - strand, within Psest_0476 at 526.252 kb on + strand at 526.252 kb on + strand at 526.379 kb on + strand at 526.380 kb on - strand at 526.380 kb on - strand at 526.526 kb on + strand at 526.730 kb on + strand at 526.751 kb on + strand at 526.751 kb on + strand at 526.751 kb on + strand at 526.751 kb on + strand at 526.751 kb on + strand at 526.751 kb on + strand at 526.751 kb on + strand at 526.752 kb on - strand at 526.828 kb on + strand at 526.828 kb on + strand at 526.951 kb on + strand, within Psest_0477 at 526.979 kb on + strand, within Psest_0477 at 526.979 kb on + strand, within Psest_0477 at 526.979 kb on + strand, within Psest_0477 at 526.979 kb on + strand, within Psest_0477 at 526.979 kb on + strand, within Psest_0477 at 526.979 kb on + strand, within Psest_0477 at 526.980 kb on - strand, within Psest_0477 at 526.980 kb on - strand, within Psest_0477 at 526.980 kb on - strand, within Psest_0477 at 526.980 kb on - strand, within Psest_0477 at 526.980 kb on - strand, within Psest_0477 at 526.980 kb on - strand, within Psest_0477 at 526.980 kb on - strand, within Psest_0477 at 526.980 kb on - strand, within Psest_0477 at 526.980 kb on - strand, within Psest_0477 at 526.980 kb on - strand, within Psest_0477 at 526.980 kb on - strand, within Psest_0477 at 527.203 kb on + strand, within Psest_0477 at 527.203 kb on + strand, within Psest_0477 at 527.203 kb on + strand, within Psest_0477 at 527.203 kb on + strand, within Psest_0477 at 527.204 kb on - strand, within Psest_0477 at 527.204 kb on - strand, within Psest_0477 at 527.204 kb on - strand, within Psest_0477 at 527.204 kb on - strand, within Psest_0477 at 527.204 kb on - strand, within Psest_0477 at 527.204 kb on - strand, within Psest_0477 at 527.204 kb on - strand, within Psest_0477 at 527.280 kb on + strand, within Psest_0477 at 527.280 kb on + strand, within Psest_0477 at 527.281 kb on - strand, within Psest_0477
Per-strain Table
Position Strand Gene LocusTag Fraction Def Tetracycline 0.0004 mg/ml remove 524,796 + +1.3 524,827 + +0.2 524,830 + -0.1 524,830 + -0.1 524,831 - +0.2 524,831 - +0.3 524,831 - -0.1 524,864 + -0.4 524,864 + +0.4 524,865 - -0.7 524,865 - +1.7 524,980 + -0.2 524,980 + -2.4 524,980 + -1.9 525,070 + Psest_0476 0.11 -2.2 525,070 + Psest_0476 0.11 -1.4 525,070 + Psest_0476 0.11 -1.4 525,071 - Psest_0476 0.11 -0.6 525,071 - Psest_0476 0.11 -1.6 525,071 - Psest_0476 0.11 -1.2 525,071 - Psest_0476 0.11 -0.2 525,071 - Psest_0476 0.11 -2.6 525,071 - Psest_0476 0.11 -1.3 525,091 + Psest_0476 0.13 +0.5 525,091 + Psest_0476 0.13 -2.1 525,091 + Psest_0476 0.13 -2.6 525,092 - Psest_0476 0.13 -2.6 525,092 - Psest_0476 0.13 -0.8 525,132 + Psest_0476 0.15 -3.5 525,132 + Psest_0476 0.15 -1.3 525,328 + Psest_0476 0.29 -0.4 525,331 + Psest_0476 0.29 -1.7 525,331 + Psest_0476 0.29 -1.1 525,332 - Psest_0476 0.29 +0.4 525,332 - Psest_0476 0.29 -2.1 525,370 + Psest_0476 0.32 -1.7 525,371 - Psest_0476 0.32 -1.3 525,371 - Psest_0476 0.32 -1.7 525,575 - Psest_0476 0.46 -0.7 525,691 + Psest_0476 0.54 -2.4 525,691 + Psest_0476 0.54 -1.9 525,691 + Psest_0476 0.54 -1.6 525,692 - Psest_0476 0.54 -0.9 525,692 - Psest_0476 0.54 -0.6 525,883 - Psest_0476 0.67 -2.0 525,922 + Psest_0476 0.70 -1.2 525,923 - Psest_0476 0.70 -1.3 526,252 + -1.7 526,252 + -1.6 526,379 + -2.4 526,380 - -1.9 526,380 - -1.1 526,526 + +0.4 526,730 + -0.6 526,751 + -2.8 526,751 + -1.8 526,751 + -0.8 526,751 + -2.0 526,751 + -1.5 526,751 + -0.6 526,751 + -1.1 526,752 - -1.2 526,828 + -0.9 526,828 + -0.6 526,951 + Psest_0477 0.11 -2.1 526,979 + Psest_0477 0.12 -0.3 526,979 + Psest_0477 0.12 -0.4 526,979 + Psest_0477 0.12 -1.6 526,979 + Psest_0477 0.12 -1.9 526,979 + Psest_0477 0.12 -2.2 526,979 + Psest_0477 0.12 -1.6 526,980 - Psest_0477 0.13 -1.4 526,980 - Psest_0477 0.13 -0.2 526,980 - Psest_0477 0.13 -1.1 526,980 - Psest_0477 0.13 -0.4 526,980 - Psest_0477 0.13 -1.4 526,980 - Psest_0477 0.13 -0.5 526,980 - Psest_0477 0.13 -1.4 526,980 - Psest_0477 0.13 -2.2 526,980 - Psest_0477 0.13 -0.4 526,980 - Psest_0477 0.13 -1.8 526,980 - Psest_0477 0.13 -1.5 527,203 + Psest_0477 0.24 -1.4 527,203 + Psest_0477 0.24 -0.7 527,203 + Psest_0477 0.24 -1.6 527,203 + Psest_0477 0.24 -2.3 527,204 - Psest_0477 0.24 -1.4 527,204 - Psest_0477 0.24 -1.7 527,204 - Psest_0477 0.24 -3.6 527,204 - Psest_0477 0.24 -2.7 527,204 - Psest_0477 0.24 -2.8 527,204 - Psest_0477 0.24 +0.4 527,204 - Psest_0477 0.24 -1.1 527,280 + Psest_0477 0.28 -0.2 527,280 + Psest_0477 0.28 -3.5 527,281 - Psest_0477 0.29 -2.4
Or see this region's nucleotide sequence