Experiment: Def Tetracycline 0.0004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0025 and Psest_0026 are separated by 155 nucleotides Psest_0026 and Psest_0027 are separated by 52 nucleotides Psest_0027 and Psest_0028 are separated by 152 nucleotides
Psest_0025: Psest_0025 - DNA protecting protein DprA, at 25,790 to 26,887
_0025
Psest_0026: Psest_0026 - Putative translation factor (SUA5), at 27,043 to 27,600
_0026
Psest_0027: Psest_0027 - NADPH:quinone reductase and related Zn-dependent oxidoreductases, at 27,653 to 28,630
_0027
Psest_0028: Psest_0028 - Coproporphyrinogen III oxidase, at 28,783 to 29,700
_0028
Position (kb) 27
28
29 Strain fitness (log2 ratio) -2
-1
0
1
2 at 26.886 kb on - strand at 26.886 kb on - strand at 26.971 kb on + strand at 26.971 kb on + strand at 26.972 kb on - strand at 26.972 kb on - strand at 27.825 kb on + strand, within Psest_0027 at 27.990 kb on + strand, within Psest_0027 at 28.040 kb on + strand, within Psest_0027 at 28.040 kb on + strand, within Psest_0027 at 28.040 kb on + strand, within Psest_0027 at 28.040 kb on + strand, within Psest_0027 at 28.040 kb on + strand, within Psest_0027 at 28.041 kb on - strand, within Psest_0027 at 28.041 kb on - strand, within Psest_0027 at 28.041 kb on - strand, within Psest_0027 at 28.041 kb on - strand, within Psest_0027 at 28.041 kb on - strand, within Psest_0027 at 28.041 kb on - strand, within Psest_0027 at 28.041 kb on - strand, within Psest_0027 at 28.041 kb on - strand, within Psest_0027 at 28.041 kb on - strand, within Psest_0027 at 28.041 kb on - strand, within Psest_0027 at 28.041 kb on - strand, within Psest_0027 at 28.066 kb on + strand, within Psest_0027 at 28.243 kb on - strand, within Psest_0027 at 28.243 kb on - strand, within Psest_0027 at 28.372 kb on - strand, within Psest_0027 at 28.477 kb on - strand, within Psest_0027 at 28.494 kb on + strand, within Psest_0027 at 28.494 kb on + strand, within Psest_0027 at 28.494 kb on + strand, within Psest_0027 at 28.494 kb on + strand, within Psest_0027 at 28.494 kb on + strand, within Psest_0027 at 28.495 kb on - strand, within Psest_0027 at 28.495 kb on - strand, within Psest_0027 at 28.703 kb on + strand at 28.703 kb on + strand at 28.704 kb on - strand at 28.704 kb on - strand at 28.704 kb on - strand at 28.704 kb on - strand at 28.704 kb on - strand at 28.750 kb on - strand at 28.813 kb on + strand at 28.813 kb on + strand at 28.813 kb on + strand at 29.086 kb on + strand, within Psest_0028 at 29.087 kb on - strand, within Psest_0028 at 29.186 kb on - strand, within Psest_0028 at 29.410 kb on + strand, within Psest_0028 at 29.410 kb on + strand, within Psest_0028 at 29.410 kb on + strand, within Psest_0028 at 29.503 kb on + strand, within Psest_0028 at 29.512 kb on + strand, within Psest_0028 at 29.512 kb on + strand, within Psest_0028 at 29.512 kb on + strand, within Psest_0028 at 29.513 kb on - strand, within Psest_0028 at 29.513 kb on - strand, within Psest_0028 at 29.513 kb on - strand, within Psest_0028 at 29.513 kb on - strand, within Psest_0028 at 29.513 kb on - strand, within Psest_0028 at 29.513 kb on - strand, within Psest_0028 at 29.524 kb on + strand, within Psest_0028 at 29.611 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Tetracycline 0.0004 mg/ml remove 26,886 - +0.1 26,886 - +0.6 26,971 + +1.6 26,971 + +1.5 26,972 - -1.3 26,972 - +1.0 27,825 + Psest_0027 0.18 -0.4 27,990 + Psest_0027 0.34 -0.2 28,040 + Psest_0027 0.40 -0.4 28,040 + Psest_0027 0.40 +1.1 28,040 + Psest_0027 0.40 -1.6 28,040 + Psest_0027 0.40 -1.1 28,040 + Psest_0027 0.40 -0.0 28,041 - Psest_0027 0.40 -0.2 28,041 - Psest_0027 0.40 -0.0 28,041 - Psest_0027 0.40 -0.0 28,041 - Psest_0027 0.40 -0.0 28,041 - Psest_0027 0.40 -0.8 28,041 - Psest_0027 0.40 +0.2 28,041 - Psest_0027 0.40 -0.3 28,041 - Psest_0027 0.40 +2.3 28,041 - Psest_0027 0.40 -0.4 28,041 - Psest_0027 0.40 -0.0 28,041 - Psest_0027 0.40 -0.3 28,066 + Psest_0027 0.42 -1.0 28,243 - Psest_0027 0.60 +1.0 28,243 - Psest_0027 0.60 -0.0 28,372 - Psest_0027 0.74 -0.5 28,477 - Psest_0027 0.84 -1.1 28,494 + Psest_0027 0.86 +0.5 28,494 + Psest_0027 0.86 -0.0 28,494 + Psest_0027 0.86 +0.3 28,494 + Psest_0027 0.86 +0.7 28,494 + Psest_0027 0.86 +1.0 28,495 - Psest_0027 0.86 -0.6 28,495 - Psest_0027 0.86 -0.6 28,703 + -0.3 28,703 + -0.1 28,704 - +0.5 28,704 - -0.6 28,704 - -1.0 28,704 - -0.4 28,704 - +1.0 28,750 - -1.8 28,813 + +0.4 28,813 + +1.2 28,813 + -1.0 29,086 + Psest_0028 0.33 +0.3 29,087 - Psest_0028 0.33 -1.0 29,186 - Psest_0028 0.44 +1.6 29,410 + Psest_0028 0.68 -0.6 29,410 + Psest_0028 0.68 +2.0 29,410 + Psest_0028 0.68 -0.6 29,503 + Psest_0028 0.78 -0.0 29,512 + Psest_0028 0.79 +0.3 29,512 + Psest_0028 0.79 -0.6 29,512 + Psest_0028 0.79 -1.0 29,513 - Psest_0028 0.80 -0.0 29,513 - Psest_0028 0.80 -0.0 29,513 - Psest_0028 0.80 +0.8 29,513 - Psest_0028 0.80 -1.4 29,513 - Psest_0028 0.80 -0.7 29,513 - Psest_0028 0.80 -2.0 29,524 + Psest_0028 0.81 +1.0 29,611 + +0.2
Or see this region's nucleotide sequence