Experiment: Def Doxycycline hyclate 0.0006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0307 and Psest_0308 are separated by 25 nucleotides Psest_0308 and Psest_0309 are separated by 262 nucleotides Psest_0309 and Psest_0310 are separated by 14 nucleotides
Psest_0307: Psest_0307 - Cytochrome B561, at 326,922 to 327,476
_0307
Psest_0308: Psest_0308 - Uncharacterized conserved protein, at 327,502 to 328,077
_0308
Psest_0309: Psest_0309 - RND family efflux transporter, MFP subunit, at 328,340 to 329,482
_0309
Psest_0310: Psest_0310 - The (Largely Gram-negative Bacterial) Hydrophobe/Amphiphile Efflux-1 (HAE1) Family, at 329,497 to 332,634
_0310
Position (kb) 328
329
330 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 327.360 kb on + strand, within Psest_0307 at 327.361 kb on - strand, within Psest_0307 at 327.394 kb on + strand, within Psest_0307 at 327.394 kb on + strand, within Psest_0307 at 327.394 kb on + strand, within Psest_0307 at 327.394 kb on + strand, within Psest_0307 at 327.394 kb on + strand, within Psest_0307 at 327.394 kb on + strand, within Psest_0307 at 327.394 kb on + strand, within Psest_0307 at 327.394 kb on + strand, within Psest_0307 at 327.395 kb on - strand, within Psest_0307 at 327.395 kb on - strand, within Psest_0307 at 327.395 kb on - strand, within Psest_0307 at 327.395 kb on - strand, within Psest_0307 at 327.395 kb on - strand, within Psest_0307 at 327.395 kb on - strand, within Psest_0307 at 327.395 kb on - strand, within Psest_0307 at 327.634 kb on + strand, within Psest_0308 at 327.634 kb on + strand, within Psest_0308 at 328.071 kb on + strand at 328.071 kb on + strand at 328.072 kb on - strand at 328.075 kb on + strand at 328.075 kb on + strand at 328.075 kb on + strand at 328.075 kb on + strand at 328.075 kb on + strand at 328.075 kb on + strand at 328.076 kb on - strand at 328.076 kb on - strand at 328.076 kb on - strand at 328.076 kb on - strand at 328.076 kb on - strand at 328.076 kb on - strand at 328.076 kb on - strand at 328.076 kb on - strand at 328.076 kb on - strand at 328.076 kb on - strand at 328.076 kb on - strand at 328.076 kb on - strand at 328.087 kb on - strand at 328.171 kb on + strand at 328.171 kb on + strand at 328.171 kb on + strand at 328.172 kb on - strand at 328.172 kb on - strand at 328.207 kb on + strand at 328.207 kb on + strand at 328.207 kb on + strand at 328.207 kb on + strand at 328.207 kb on + strand at 328.207 kb on + strand at 328.207 kb on + strand at 328.208 kb on - strand at 328.208 kb on - strand at 328.254 kb on + strand at 328.254 kb on + strand at 328.254 kb on + strand at 328.254 kb on + strand at 328.254 kb on + strand at 328.254 kb on + strand at 328.254 kb on + strand at 328.254 kb on + strand at 328.255 kb on - strand at 328.258 kb on + strand at 328.258 kb on + strand at 328.260 kb on + strand at 328.260 kb on + strand at 328.260 kb on + strand at 328.261 kb on - strand at 328.736 kb on + strand, within Psest_0309 at 328.926 kb on - strand, within Psest_0309 at 329.162 kb on - strand, within Psest_0309 at 329.491 kb on + strand at 329.491 kb on + strand at 329.491 kb on + strand at 329.965 kb on + strand, within Psest_0310 at 330.104 kb on + strand, within Psest_0310 at 330.105 kb on - strand, within Psest_0310 at 330.105 kb on - strand, within Psest_0310 at 330.105 kb on - strand, within Psest_0310 at 330.105 kb on - strand, within Psest_0310 at 330.105 kb on - strand, within Psest_0310 at 330.413 kb on + strand, within Psest_0310 at 330.414 kb on - strand, within Psest_0310 at 330.475 kb on + strand, within Psest_0310
Per-strain Table
Position Strand Gene LocusTag Fraction Def Doxycycline hyclate 0.0006 mg/ml remove 327,360 + Psest_0307 0.79 +0.8 327,361 - Psest_0307 0.79 +0.5 327,394 + Psest_0307 0.85 +0.6 327,394 + Psest_0307 0.85 +0.8 327,394 + Psest_0307 0.85 -0.2 327,394 + Psest_0307 0.85 +1.8 327,394 + Psest_0307 0.85 +0.5 327,394 + Psest_0307 0.85 +0.3 327,394 + Psest_0307 0.85 +1.8 327,394 + Psest_0307 0.85 +1.4 327,395 - Psest_0307 0.85 -1.7 327,395 - Psest_0307 0.85 +0.1 327,395 - Psest_0307 0.85 -0.6 327,395 - Psest_0307 0.85 +0.7 327,395 - Psest_0307 0.85 +0.9 327,395 - Psest_0307 0.85 -0.6 327,395 - Psest_0307 0.85 -0.1 327,634 + Psest_0308 0.23 +0.5 327,634 + Psest_0308 0.23 +0.4 328,071 + +1.4 328,071 + +2.8 328,072 - +0.6 328,075 + +1.2 328,075 + +0.8 328,075 + +1.1 328,075 + +1.8 328,075 + +1.8 328,075 + +2.1 328,076 - +0.2 328,076 - -1.3 328,076 - -0.8 328,076 - +0.6 328,076 - -0.1 328,076 - -0.1 328,076 - +0.8 328,076 - +0.4 328,076 - +1.2 328,076 - -0.5 328,076 - +0.1 328,076 - -1.2 328,087 - -2.2 328,171 + +2.6 328,171 + +4.3 328,171 + +5.5 328,172 - -0.9 328,172 - -1.1 328,207 + +1.9 328,207 + +2.6 328,207 + +2.5 328,207 + +3.3 328,207 + +2.4 328,207 + +3.8 328,207 + +1.4 328,208 - -0.4 328,208 - -1.5 328,254 + +1.9 328,254 + +3.0 328,254 + +1.6 328,254 + +2.2 328,254 + +0.2 328,254 + +0.5 328,254 + +1.8 328,254 + +1.4 328,255 - -0.4 328,258 + +2.1 328,258 + +1.5 328,260 + +0.4 328,260 + +2.0 328,260 + -0.7 328,261 - +0.4 328,736 + Psest_0309 0.35 +1.8 328,926 - Psest_0309 0.51 -2.3 329,162 - Psest_0309 0.72 +1.2 329,491 + -0.8 329,491 + +0.8 329,491 + -1.1 329,965 + Psest_0310 0.15 +1.4 330,104 + Psest_0310 0.19 -0.2 330,105 - Psest_0310 0.19 -1.4 330,105 - Psest_0310 0.19 -0.6 330,105 - Psest_0310 0.19 +0.2 330,105 - Psest_0310 0.19 -1.6 330,105 - Psest_0310 0.19 +0.3 330,413 + Psest_0310 0.29 +1.2 330,414 - Psest_0310 0.29 -1.1 330,475 + Psest_0310 0.31 +0.2
Or see this region's nucleotide sequence