Experiment: Def Doxycycline hyclate 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0717 and Psest_0718 are separated by 429 nucleotides Psest_0718 and Psest_0719 are separated by 48 nucleotides Psest_0719 and Psest_0720 are separated by 138 nucleotides
Psest_0717: Psest_0717 - Protein of unknown function (DUF1631)., at 785,114 to 787,372
_0717
Psest_0718: Psest_0718 - nicotinate-nucleotide pyrophosphorylase, at 787,802 to 788,650
_0718
Psest_0719: Psest_0719 - Uncharacterized protein conserved in bacteria, at 788,699 to 789,130
_0719
Psest_0720: Psest_0720 - Predicted hydrolase of the alpha/beta superfamily, at 789,269 to 789,895
_0720
Position (kb) 787
788
789 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 787.004 kb on + strand, within Psest_0717 at 787.005 kb on - strand, within Psest_0717 at 787.005 kb on - strand, within Psest_0717 at 787.230 kb on - strand at 787.397 kb on + strand at 787.397 kb on + strand at 787.398 kb on - strand at 787.438 kb on - strand at 787.440 kb on + strand at 787.440 kb on + strand at 787.440 kb on + strand at 787.440 kb on + strand at 787.440 kb on + strand at 787.445 kb on + strand at 787.445 kb on + strand at 787.494 kb on + strand at 787.494 kb on + strand at 787.494 kb on + strand at 787.494 kb on + strand at 787.494 kb on + strand at 787.494 kb on + strand at 787.494 kb on + strand at 787.494 kb on + strand at 787.495 kb on - strand at 787.495 kb on - strand at 787.556 kb on + strand at 787.557 kb on - strand at 787.618 kb on + strand at 787.619 kb on - strand at 787.619 kb on - strand at 787.620 kb on + strand at 787.620 kb on + strand at 787.620 kb on + strand at 787.620 kb on + strand at 787.620 kb on + strand at 787.620 kb on + strand at 787.620 kb on + strand at 787.620 kb on + strand at 787.620 kb on + strand at 787.620 kb on + strand at 787.620 kb on + strand at 787.620 kb on + strand at 787.620 kb on + strand at 787.620 kb on + strand at 787.620 kb on + strand at 787.620 kb on + strand at 787.620 kb on + strand at 787.620 kb on + strand at 787.620 kb on + strand at 787.620 kb on + strand at 787.620 kb on + strand at 787.620 kb on + strand at 787.621 kb on - strand at 787.621 kb on - strand at 787.772 kb on + strand at 787.772 kb on + strand at 787.772 kb on + strand at 787.772 kb on + strand at 787.772 kb on + strand at 787.772 kb on + strand at 787.773 kb on - strand at 787.775 kb on - strand at 787.775 kb on - strand at 787.775 kb on - strand at 787.851 kb on + strand at 787.851 kb on + strand at 787.851 kb on + strand at 787.851 kb on + strand at 787.851 kb on + strand at 787.851 kb on + strand at 787.851 kb on + strand at 787.851 kb on + strand at 787.851 kb on + strand at 787.851 kb on + strand at 787.852 kb on - strand at 787.852 kb on - strand at 787.852 kb on - strand at 787.852 kb on - strand at 787.852 kb on - strand at 788.165 kb on + strand, within Psest_0718 at 788.246 kb on + strand, within Psest_0718 at 788.246 kb on + strand, within Psest_0718 at 788.291 kb on + strand, within Psest_0718 at 788.291 kb on + strand, within Psest_0718 at 788.305 kb on + strand, within Psest_0718 at 788.305 kb on + strand, within Psest_0718 at 788.306 kb on - strand, within Psest_0718 at 788.447 kb on - strand, within Psest_0718 at 788.582 kb on + strand at 788.582 kb on + strand at 788.582 kb on + strand at 788.582 kb on + strand at 788.582 kb on + strand at 788.582 kb on + strand at 788.582 kb on + strand at 788.582 kb on + strand at 788.582 kb on + strand at 788.582 kb on + strand at 788.582 kb on + strand at 788.582 kb on + strand at 788.582 kb on + strand at 788.582 kb on + strand at 788.582 kb on + strand at 788.583 kb on - strand at 788.583 kb on - strand at 788.583 kb on - strand at 788.583 kb on - strand at 788.583 kb on - strand at 788.583 kb on - strand at 788.583 kb on - strand at 788.583 kb on - strand at 788.583 kb on - strand at 788.583 kb on - strand at 788.583 kb on - strand at 788.583 kb on - strand at 788.583 kb on - strand at 788.583 kb on - strand at 788.583 kb on - strand at 788.583 kb on - strand at 788.583 kb on - strand at 788.583 kb on - strand at 788.583 kb on - strand at 788.584 kb on + strand at 788.584 kb on + strand at 788.584 kb on + strand at 788.584 kb on + strand at 788.584 kb on + strand at 788.584 kb on + strand at 788.584 kb on + strand at 788.584 kb on + strand at 788.585 kb on - strand at 788.585 kb on - strand at 788.585 kb on - strand at 788.585 kb on - strand at 788.585 kb on - strand at 788.585 kb on - strand at 788.585 kb on - strand at 788.585 kb on - strand at 788.585 kb on - strand at 788.585 kb on - strand at 788.629 kb on - strand at 788.900 kb on + strand, within Psest_0719 at 788.900 kb on + strand, within Psest_0719 at 788.922 kb on + strand, within Psest_0719 at 788.923 kb on - strand, within Psest_0719 at 788.923 kb on - strand, within Psest_0719 at 788.926 kb on + strand, within Psest_0719 at 788.926 kb on + strand, within Psest_0719 at 788.926 kb on + strand, within Psest_0719 at 788.988 kb on + strand, within Psest_0719 at 788.989 kb on - strand, within Psest_0719 at 788.989 kb on - strand, within Psest_0719 at 788.989 kb on - strand, within Psest_0719 at 788.989 kb on - strand, within Psest_0719 at 789.031 kb on + strand, within Psest_0719 at 789.032 kb on - strand, within Psest_0719 at 789.032 kb on - strand, within Psest_0719 at 789.143 kb on + strand at 789.143 kb on + strand at 789.143 kb on + strand at 789.143 kb on + strand at 789.144 kb on - strand at 789.144 kb on - strand at 789.144 kb on - strand at 789.144 kb on - strand at 789.144 kb on - strand at 789.144 kb on - strand at 789.145 kb on + strand at 789.145 kb on + strand at 789.145 kb on + strand at 789.146 kb on - strand at 789.146 kb on - strand at 789.146 kb on - strand at 789.146 kb on - strand at 789.147 kb on + strand at 789.147 kb on + strand at 789.147 kb on + strand at 789.147 kb on + strand at 789.147 kb on + strand at 789.147 kb on + strand at 789.147 kb on + strand at 789.148 kb on - strand at 789.148 kb on - strand at 789.148 kb on - strand at 789.148 kb on - strand at 789.148 kb on - strand at 789.148 kb on - strand at 789.148 kb on - strand at 789.148 kb on - strand at 789.148 kb on - strand at 789.148 kb on - strand at 789.148 kb on - strand at 789.161 kb on + strand at 789.161 kb on + strand at 789.161 kb on + strand at 789.161 kb on + strand at 789.161 kb on + strand at 789.161 kb on + strand at 789.161 kb on + strand at 789.161 kb on + strand at 789.162 kb on - strand at 789.162 kb on - strand at 789.162 kb on - strand at 789.162 kb on - strand at 789.162 kb on - strand at 789.162 kb on - strand at 789.162 kb on - strand at 789.162 kb on - strand at 789.162 kb on - strand at 789.162 kb on - strand at 789.176 kb on + strand at 789.187 kb on + strand at 789.188 kb on - strand at 789.188 kb on - strand at 789.188 kb on - strand at 789.188 kb on - strand at 789.188 kb on - strand at 789.188 kb on - strand at 789.188 kb on - strand at 789.221 kb on + strand at 789.221 kb on + strand at 789.221 kb on + strand at 789.221 kb on + strand at 789.221 kb on + strand at 789.221 kb on + strand at 789.221 kb on + strand at 789.221 kb on + strand at 789.221 kb on + strand at 789.221 kb on + strand at 789.222 kb on - strand at 789.222 kb on - strand at 789.222 kb on - strand at 789.222 kb on - strand at 789.225 kb on + strand at 789.225 kb on + strand at 789.225 kb on + strand at 789.225 kb on + strand at 789.226 kb on - strand at 789.226 kb on - strand at 789.226 kb on - strand at 789.226 kb on - strand at 789.226 kb on - strand at 789.226 kb on - strand at 789.330 kb on - strand at 789.330 kb on - strand at 789.449 kb on + strand, within Psest_0720 at 789.470 kb on + strand, within Psest_0720 at 789.471 kb on - strand, within Psest_0720
Per-strain Table
Position Strand Gene LocusTag Fraction Def Doxycycline hyclate 0.0002 mg/ml remove 787,004 + Psest_0717 0.84 -0.1 787,005 - Psest_0717 0.84 -0.3 787,005 - Psest_0717 0.84 -0.6 787,230 - +0.1 787,397 + -0.1 787,397 + -0.9 787,398 - +0.5 787,438 - +0.4 787,440 + +0.7 787,440 + -0.2 787,440 + -0.3 787,440 + +0.0 787,440 + -0.1 787,445 + +0.3 787,445 + -0.1 787,494 + -0.4 787,494 + -0.8 787,494 + -0.0 787,494 + -0.3 787,494 + +0.0 787,494 + +0.3 787,494 + -1.1 787,494 + -0.4 787,495 - -0.6 787,495 - -1.1 787,556 + -0.5 787,557 - -1.7 787,618 + -0.5 787,619 - -1.7 787,619 - -3.0 787,620 + +0.1 787,620 + +0.6 787,620 + +0.7 787,620 + +0.5 787,620 + +1.0 787,620 + -0.3 787,620 + -0.0 787,620 + +0.9 787,620 + +0.3 787,620 + +0.5 787,620 + +0.2 787,620 + +0.4 787,620 + +1.2 787,620 + +0.6 787,620 + -0.2 787,620 + -0.3 787,620 + +0.2 787,620 + -0.1 787,620 + +0.9 787,620 + +0.5 787,620 + -0.1 787,620 + +0.6 787,621 - -3.1 787,621 - -3.8 787,772 + +0.4 787,772 + +1.2 787,772 + +0.8 787,772 + +0.1 787,772 + -0.3 787,772 + +0.3 787,773 - -1.0 787,775 - -2.3 787,775 - -1.7 787,775 - -2.1 787,851 + -1.5 787,851 + -2.9 787,851 + -4.0 787,851 + -1.6 787,851 + -2.2 787,851 + -1.9 787,851 + -0.9 787,851 + -3.4 787,851 + -1.5 787,851 + -2.4 787,852 - -1.8 787,852 - -4.5 787,852 - -4.2 787,852 - -3.3 787,852 - -3.1 788,165 + Psest_0718 0.43 -4.3 788,246 + Psest_0718 0.52 -1.7 788,246 + Psest_0718 0.52 -4.7 788,291 + Psest_0718 0.58 -2.1 788,291 + Psest_0718 0.58 -0.9 788,305 + Psest_0718 0.59 -3.5 788,305 + Psest_0718 0.59 -1.7 788,306 - Psest_0718 0.59 -2.2 788,447 - Psest_0718 0.76 +0.5 788,582 + -3.0 788,582 + -1.5 788,582 + -2.8 788,582 + -0.9 788,582 + -3.7 788,582 + -2.0 788,582 + -0.7 788,582 + -4.0 788,582 + -3.3 788,582 + -4.3 788,582 + -2.8 788,582 + -2.7 788,582 + -4.1 788,582 + -3.4 788,582 + -3.6 788,583 - +0.5 788,583 - -3.4 788,583 - -2.5 788,583 - -2.2 788,583 - -3.9 788,583 - -2.1 788,583 - -1.7 788,583 - -2.7 788,583 - -3.1 788,583 - -2.2 788,583 - -2.2 788,583 - -1.9 788,583 - -3.3 788,583 - -3.1 788,583 - -1.4 788,583 - -5.1 788,583 - -2.8 788,583 - -2.2 788,583 - -2.5 788,584 + -2.8 788,584 + -2.2 788,584 + -3.0 788,584 + -3.6 788,584 + -1.9 788,584 + -1.1 788,584 + -2.8 788,584 + -3.6 788,585 - -4.4 788,585 - -3.1 788,585 - -2.9 788,585 - -4.1 788,585 - -4.3 788,585 - -2.2 788,585 - -2.5 788,585 - -2.4 788,585 - -2.4 788,585 - -3.9 788,629 - -2.7 788,900 + Psest_0719 0.47 -0.9 788,900 + Psest_0719 0.47 -2.0 788,922 + Psest_0719 0.52 -0.3 788,923 - Psest_0719 0.52 -1.3 788,923 - Psest_0719 0.52 -0.5 788,926 + Psest_0719 0.53 +1.5 788,926 + Psest_0719 0.53 -1.2 788,926 + Psest_0719 0.53 +0.0 788,988 + Psest_0719 0.67 -0.5 788,989 - Psest_0719 0.67 +1.3 788,989 - Psest_0719 0.67 +0.2 788,989 - Psest_0719 0.67 -0.0 788,989 - Psest_0719 0.67 -0.9 789,031 + Psest_0719 0.77 -2.3 789,032 - Psest_0719 0.77 -0.8 789,032 - Psest_0719 0.77 -0.1 789,143 + -0.5 789,143 + -0.7 789,143 + -1.1 789,143 + -0.9 789,144 - -0.9 789,144 - +0.2 789,144 - +0.5 789,144 - -0.2 789,144 - -0.5 789,144 - -1.0 789,145 + -0.4 789,145 + -1.5 789,145 + +0.0 789,146 - -1.6 789,146 - -0.1 789,146 - -1.4 789,146 - -1.1 789,147 + -1.1 789,147 + -0.8 789,147 + -0.0 789,147 + -0.4 789,147 + -1.0 789,147 + -0.4 789,147 + -0.2 789,148 - +0.0 789,148 - -0.7 789,148 - -1.5 789,148 - +0.2 789,148 - -0.8 789,148 - -0.3 789,148 - -2.4 789,148 - +0.2 789,148 - +0.1 789,148 - +0.1 789,148 - +0.1 789,161 + -1.5 789,161 + -0.0 789,161 + +0.4 789,161 + -0.8 789,161 + -0.3 789,161 + -2.2 789,161 + -0.5 789,161 + -0.7 789,162 - +0.1 789,162 - +1.1 789,162 - -0.3 789,162 - -0.8 789,162 - -1.2 789,162 - -0.5 789,162 - +0.6 789,162 - -0.5 789,162 - -2.3 789,162 - -0.0 789,176 + +0.4 789,187 + -0.8 789,188 - +0.7 789,188 - +0.2 789,188 - -0.0 789,188 - +0.5 789,188 - +0.3 789,188 - -1.2 789,188 - -0.7 789,221 + +1.0 789,221 + -1.2 789,221 + -0.5 789,221 + -1.3 789,221 + -1.3 789,221 + -0.3 789,221 + -0.5 789,221 + -1.4 789,221 + -0.9 789,221 + +0.1 789,222 - +1.1 789,222 - -0.6 789,222 - +0.6 789,222 - -0.3 789,225 + -0.9 789,225 + -0.4 789,225 + -0.7 789,225 + -0.7 789,226 - -0.4 789,226 - -0.6 789,226 - -0.9 789,226 - -0.5 789,226 - +0.4 789,226 - +0.3 789,330 - +0.5 789,330 - +0.2 789,449 + Psest_0720 0.29 +0.9 789,470 + Psest_0720 0.32 -0.4 789,471 - Psest_0720 0.32 +0.8
Or see this region's nucleotide sequence