Experiment: Def Neomycin 0.0008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0968 and Psest_0969 overlap by 4 nucleotides Psest_0969 and Psest_0970 overlap by 23 nucleotides Psest_0970 and Psest_0971 are separated by 35 nucleotides Psest_0971 and Psest_0972 are separated by 532 nucleotides
Psest_0968: Psest_0968 - carbamoyl-phosphate synthase, large subunit, at 1,049,806 to 1,053,027
_0968
Psest_0969: Psest_0969 - transcription elongation factor GreA, at 1,053,024 to 1,053,500
_0969
Psest_0970: Psest_0970 - hypothetical protein, at 1,053,478 to 1,053,909
_0970
Psest_0971: Psest_0971 - Predicted RNA-binding protein containing KH domain, possibly ribosomal protein, at 1,053,945 to 1,054,256
_0971
Psest_0972: Psest_0972 - ATP-dependent metalloprotease FtsH, at 1,054,789 to 1,056,708
_0972
Position (kb)
1053
1054 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1053.302 kb on + strand, within Psest_0969 at 1053.336 kb on + strand, within Psest_0969 at 1053.336 kb on + strand, within Psest_0969 at 1053.336 kb on + strand, within Psest_0969 at 1053.336 kb on + strand, within Psest_0969 at 1053.494 kb on + strand at 1053.495 kb on - strand at 1053.495 kb on - strand at 1053.844 kb on + strand, within Psest_0970 at 1053.844 kb on + strand, within Psest_0970 at 1053.844 kb on + strand, within Psest_0970 at 1053.844 kb on + strand, within Psest_0970 at 1053.845 kb on - strand, within Psest_0970 at 1054.226 kb on - strand at 1054.257 kb on - strand at 1054.785 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Neomycin 0.0008 mg/ml remove 1,053,302 + Psest_0969 0.58 +0.1 1,053,336 + Psest_0969 0.65 -1.2 1,053,336 + Psest_0969 0.65 -2.5 1,053,336 + Psest_0969 0.65 -0.7 1,053,336 + Psest_0969 0.65 -1.7 1,053,494 + +0.3 1,053,495 - -0.6 1,053,495 - +1.2 1,053,844 + Psest_0970 0.85 -1.9 1,053,844 + Psest_0970 0.85 -0.8 1,053,844 + Psest_0970 0.85 -1.7 1,053,844 + Psest_0970 0.85 -2.3 1,053,845 - Psest_0970 0.85 -2.8 1,054,226 - -1.6 1,054,257 - -1.2 1,054,785 + +0.5
Or see this region's nucleotide sequence