Experiment: Def Neomycin 0.0008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0886 and Psest_0888 are separated by 502 nucleotides Psest_0888 and Psest_0889 are separated by 185 nucleotides Psest_0889 and Psest_0890 are separated by 43 nucleotides
Psest_0886: Psest_0886 - Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain, at 963,442 to 964,455
_0886
Psest_0888: Psest_0888 - Uncharacterized membrane-anchored protein conserved in bacteria, at 964,958 to 966,293
_0888
Psest_0889: Psest_0889 - Lactoylglutathione lyase and related lyases, at 966,479 to 966,877
_0889
Psest_0890: Psest_0890 - S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase, at 966,921 to 968,030
_0890
Position (kb) 964
965
966
967 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 964.065 kb on - strand, within Psest_0886 at 964.067 kb on + strand, within Psest_0886 at 964.067 kb on + strand, within Psest_0886 at 964.067 kb on + strand, within Psest_0886 at 964.067 kb on + strand, within Psest_0886 at 964.068 kb on - strand, within Psest_0886 at 964.075 kb on + strand, within Psest_0886 at 964.076 kb on - strand, within Psest_0886 at 964.289 kb on - strand, within Psest_0886 at 964.289 kb on - strand, within Psest_0886 at 964.472 kb on + strand at 964.472 kb on + strand at 964.472 kb on + strand at 964.472 kb on + strand at 964.472 kb on + strand at 964.472 kb on + strand at 964.473 kb on - strand at 964.473 kb on - strand at 964.473 kb on - strand at 964.473 kb on - strand at 964.473 kb on - strand at 964.473 kb on - strand at 964.473 kb on - strand at 964.473 kb on - strand at 964.473 kb on - strand at 964.473 kb on - strand at 964.476 kb on + strand at 964.476 kb on + strand at 964.476 kb on + strand at 964.476 kb on + strand at 964.476 kb on + strand at 964.476 kb on + strand at 964.476 kb on + strand at 964.476 kb on + strand at 964.477 kb on - strand at 964.477 kb on - strand at 964.477 kb on - strand at 964.538 kb on + strand at 964.539 kb on - strand at 964.551 kb on + strand at 964.552 kb on - strand at 964.552 kb on - strand at 964.606 kb on + strand at 964.729 kb on + strand at 964.729 kb on + strand at 964.729 kb on + strand at 964.729 kb on + strand at 964.729 kb on + strand at 964.729 kb on + strand at 964.729 kb on + strand at 964.730 kb on - strand at 964.730 kb on - strand at 964.730 kb on - strand at 964.730 kb on - strand at 964.730 kb on - strand at 964.730 kb on - strand at 964.730 kb on - strand at 964.730 kb on - strand at 964.745 kb on + strand at 964.745 kb on + strand at 964.745 kb on + strand at 964.771 kb on + strand at 964.772 kb on - strand at 964.830 kb on + strand at 964.837 kb on + strand at 964.837 kb on + strand at 964.838 kb on - strand at 964.838 kb on - strand at 965.045 kb on + strand at 965.046 kb on - strand at 965.127 kb on + strand, within Psest_0888 at 965.128 kb on - strand, within Psest_0888 at 965.128 kb on - strand, within Psest_0888 at 965.431 kb on - strand, within Psest_0888 at 965.431 kb on - strand, within Psest_0888 at 965.431 kb on - strand, within Psest_0888 at 965.431 kb on - strand, within Psest_0888 at 965.431 kb on - strand, within Psest_0888 at 965.434 kb on - strand, within Psest_0888 at 965.434 kb on - strand, within Psest_0888 at 965.434 kb on - strand, within Psest_0888 at 965.434 kb on - strand, within Psest_0888 at 965.434 kb on - strand, within Psest_0888 at 965.434 kb on - strand, within Psest_0888 at 965.540 kb on + strand, within Psest_0888 at 965.551 kb on + strand, within Psest_0888 at 965.551 kb on + strand, within Psest_0888 at 965.552 kb on - strand, within Psest_0888 at 965.552 kb on - strand, within Psest_0888 at 965.647 kb on + strand, within Psest_0888 at 965.647 kb on + strand, within Psest_0888 at 965.647 kb on + strand, within Psest_0888 at 965.648 kb on - strand, within Psest_0888 at 965.648 kb on - strand, within Psest_0888 at 965.648 kb on - strand, within Psest_0888 at 965.648 kb on - strand, within Psest_0888 at 965.652 kb on + strand, within Psest_0888 at 965.653 kb on - strand, within Psest_0888 at 965.653 kb on - strand, within Psest_0888 at 965.653 kb on - strand, within Psest_0888 at 965.669 kb on + strand, within Psest_0888 at 965.789 kb on + strand, within Psest_0888 at 965.789 kb on + strand, within Psest_0888 at 965.910 kb on + strand, within Psest_0888 at 965.910 kb on + strand, within Psest_0888 at 965.911 kb on - strand, within Psest_0888 at 965.974 kb on + strand, within Psest_0888 at 965.974 kb on + strand, within Psest_0888 at 965.974 kb on + strand, within Psest_0888 at 965.974 kb on + strand, within Psest_0888 at 965.974 kb on + strand, within Psest_0888 at 965.974 kb on + strand, within Psest_0888 at 965.974 kb on + strand, within Psest_0888 at 965.974 kb on + strand, within Psest_0888 at 965.974 kb on + strand, within Psest_0888 at 965.975 kb on - strand, within Psest_0888 at 965.975 kb on - strand, within Psest_0888 at 965.975 kb on - strand, within Psest_0888 at 965.975 kb on - strand, within Psest_0888 at 965.975 kb on - strand, within Psest_0888 at 965.975 kb on - strand, within Psest_0888 at 965.975 kb on - strand, within Psest_0888 at 965.975 kb on - strand, within Psest_0888 at 965.975 kb on - strand, within Psest_0888 at 965.975 kb on - strand, within Psest_0888 at 965.975 kb on - strand, within Psest_0888 at 965.975 kb on - strand, within Psest_0888 at 965.975 kb on - strand, within Psest_0888 at 965.978 kb on + strand, within Psest_0888 at 965.978 kb on + strand, within Psest_0888 at 965.979 kb on - strand, within Psest_0888 at 965.979 kb on - strand, within Psest_0888 at 965.979 kb on - strand, within Psest_0888 at 965.979 kb on - strand, within Psest_0888 at 965.979 kb on - strand, within Psest_0888 at 965.979 kb on - strand, within Psest_0888 at 965.979 kb on - strand, within Psest_0888 at 965.979 kb on - strand, within Psest_0888 at 965.979 kb on - strand, within Psest_0888 at 966.081 kb on + strand, within Psest_0888 at 966.081 kb on + strand, within Psest_0888 at 966.081 kb on + strand, within Psest_0888 at 966.081 kb on + strand, within Psest_0888 at 966.082 kb on - strand, within Psest_0888 at 966.082 kb on - strand, within Psest_0888 at 966.144 kb on + strand, within Psest_0888 at 966.144 kb on + strand, within Psest_0888 at 966.144 kb on + strand, within Psest_0888 at 966.145 kb on - strand, within Psest_0888 at 966.145 kb on - strand, within Psest_0888 at 966.145 kb on - strand, within Psest_0888 at 966.145 kb on - strand, within Psest_0888 at 966.145 kb on - strand, within Psest_0888 at 966.145 kb on - strand, within Psest_0888 at 966.145 kb on - strand, within Psest_0888 at 966.146 kb on + strand, within Psest_0888 at 966.270 kb on - strand at 966.270 kb on - strand at 966.414 kb on - strand at 966.428 kb on - strand at 966.430 kb on - strand at 966.430 kb on - strand at 966.430 kb on - strand at 966.430 kb on - strand at 966.430 kb on - strand at 966.430 kb on - strand at 966.432 kb on + strand at 966.433 kb on - strand at 966.433 kb on - strand at 966.433 kb on - strand at 966.433 kb on - strand at 966.492 kb on + strand at 966.492 kb on + strand at 966.493 kb on - strand at 966.528 kb on + strand, within Psest_0889 at 966.529 kb on - strand, within Psest_0889 at 966.529 kb on - strand, within Psest_0889 at 966.529 kb on - strand, within Psest_0889 at 966.529 kb on - strand, within Psest_0889 at 966.537 kb on + strand, within Psest_0889 at 966.537 kb on + strand, within Psest_0889 at 966.537 kb on + strand, within Psest_0889 at 966.537 kb on + strand, within Psest_0889 at 966.537 kb on + strand, within Psest_0889 at 966.537 kb on + strand, within Psest_0889 at 966.538 kb on - strand, within Psest_0889 at 966.538 kb on - strand, within Psest_0889 at 966.731 kb on + strand, within Psest_0889 at 966.792 kb on + strand, within Psest_0889 at 966.792 kb on + strand, within Psest_0889 at 966.793 kb on - strand, within Psest_0889 at 966.793 kb on - strand, within Psest_0889 at 966.881 kb on + strand at 966.881 kb on + strand at 966.882 kb on - strand at 966.882 kb on - strand at 966.882 kb on - strand at 966.882 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Neomycin 0.0008 mg/ml remove 964,065 - Psest_0886 0.61 -0.6 964,067 + Psest_0886 0.62 -1.4 964,067 + Psest_0886 0.62 -1.4 964,067 + Psest_0886 0.62 +2.1 964,067 + Psest_0886 0.62 +0.9 964,068 - Psest_0886 0.62 -1.4 964,075 + Psest_0886 0.62 -1.7 964,076 - Psest_0886 0.63 -1.7 964,289 - Psest_0886 0.84 -0.6 964,289 - Psest_0886 0.84 +0.4 964,472 + +2.3 964,472 + -0.0 964,472 + -0.1 964,472 + -0.5 964,472 + -1.0 964,472 + -0.6 964,473 - +1.7 964,473 - +1.0 964,473 - +0.8 964,473 - +0.3 964,473 - -0.3 964,473 - -2.0 964,473 - +0.0 964,473 - -0.7 964,473 - -0.4 964,473 - -0.2 964,476 + -1.3 964,476 + +0.3 964,476 + -1.4 964,476 + +0.1 964,476 + -0.8 964,476 + -0.4 964,476 + -2.3 964,476 + +0.8 964,477 - -0.1 964,477 - -1.3 964,477 - -0.9 964,538 + +1.0 964,539 - +0.7 964,551 + +0.5 964,552 - -1.8 964,552 - -0.5 964,606 + +1.1 964,729 + +2.5 964,729 + -0.1 964,729 + +1.0 964,729 + -1.6 964,729 + -0.3 964,729 + -0.6 964,729 + +1.1 964,730 - -0.9 964,730 - -1.0 964,730 - +0.8 964,730 - -0.6 964,730 - +0.9 964,730 - +1.1 964,730 - +1.2 964,730 - +0.4 964,745 + +0.3 964,745 + +0.2 964,745 + +0.2 964,771 + +0.6 964,772 - +0.7 964,830 + +1.0 964,837 + -3.0 964,837 + +1.7 964,838 - -1.1 964,838 - +0.9 965,045 + +0.1 965,046 - +0.1 965,127 + Psest_0888 0.13 -0.6 965,128 - Psest_0888 0.13 +0.8 965,128 - Psest_0888 0.13 -0.5 965,431 - Psest_0888 0.35 -1.6 965,431 - Psest_0888 0.35 -3.3 965,431 - Psest_0888 0.35 -0.9 965,431 - Psest_0888 0.35 -0.9 965,431 - Psest_0888 0.35 +0.5 965,434 - Psest_0888 0.36 +0.3 965,434 - Psest_0888 0.36 -2.7 965,434 - Psest_0888 0.36 +0.4 965,434 - Psest_0888 0.36 -1.6 965,434 - Psest_0888 0.36 +0.9 965,434 - Psest_0888 0.36 -1.2 965,540 + Psest_0888 0.44 -1.3 965,551 + Psest_0888 0.44 +0.9 965,551 + Psest_0888 0.44 -0.3 965,552 - Psest_0888 0.44 -1.2 965,552 - Psest_0888 0.44 -0.1 965,647 + Psest_0888 0.52 -1.0 965,647 + Psest_0888 0.52 -1.8 965,647 + Psest_0888 0.52 -0.3 965,648 - Psest_0888 0.52 -0.1 965,648 - Psest_0888 0.52 +0.8 965,648 - Psest_0888 0.52 -1.5 965,648 - Psest_0888 0.52 -3.2 965,652 + Psest_0888 0.52 -0.2 965,653 - Psest_0888 0.52 -1.9 965,653 - Psest_0888 0.52 -0.1 965,653 - Psest_0888 0.52 -1.6 965,669 + Psest_0888 0.53 +0.0 965,789 + Psest_0888 0.62 -0.2 965,789 + Psest_0888 0.62 +1.3 965,910 + Psest_0888 0.71 +2.0 965,910 + Psest_0888 0.71 -0.9 965,911 - Psest_0888 0.71 +2.3 965,974 + Psest_0888 0.76 -0.3 965,974 + Psest_0888 0.76 -1.5 965,974 + Psest_0888 0.76 -0.1 965,974 + Psest_0888 0.76 +0.5 965,974 + Psest_0888 0.76 +1.2 965,974 + Psest_0888 0.76 +0.0 965,974 + Psest_0888 0.76 -2.5 965,974 + Psest_0888 0.76 -2.3 965,974 + Psest_0888 0.76 +0.7 965,975 - Psest_0888 0.76 +1.2 965,975 - Psest_0888 0.76 -2.1 965,975 - Psest_0888 0.76 -1.1 965,975 - Psest_0888 0.76 -0.8 965,975 - Psest_0888 0.76 -0.0 965,975 - Psest_0888 0.76 +0.2 965,975 - Psest_0888 0.76 -2.2 965,975 - Psest_0888 0.76 +0.9 965,975 - Psest_0888 0.76 +0.1 965,975 - Psest_0888 0.76 -1.3 965,975 - Psest_0888 0.76 +1.0 965,975 - Psest_0888 0.76 -1.0 965,975 - Psest_0888 0.76 -0.5 965,978 + Psest_0888 0.76 +0.5 965,978 + Psest_0888 0.76 +0.1 965,979 - Psest_0888 0.76 -0.6 965,979 - Psest_0888 0.76 -0.5 965,979 - Psest_0888 0.76 +0.1 965,979 - Psest_0888 0.76 +2.6 965,979 - Psest_0888 0.76 +0.8 965,979 - Psest_0888 0.76 -0.3 965,979 - Psest_0888 0.76 +2.6 965,979 - Psest_0888 0.76 -1.4 965,979 - Psest_0888 0.76 -0.4 966,081 + Psest_0888 0.84 +0.5 966,081 + Psest_0888 0.84 -0.2 966,081 + Psest_0888 0.84 +0.8 966,081 + Psest_0888 0.84 -2.1 966,082 - Psest_0888 0.84 -0.2 966,082 - Psest_0888 0.84 -3.0 966,144 + Psest_0888 0.89 -0.8 966,144 + Psest_0888 0.89 -0.8 966,144 + Psest_0888 0.89 -3.1 966,145 - Psest_0888 0.89 +0.3 966,145 - Psest_0888 0.89 -1.4 966,145 - Psest_0888 0.89 -1.8 966,145 - Psest_0888 0.89 +1.2 966,145 - Psest_0888 0.89 +1.4 966,145 - Psest_0888 0.89 +0.7 966,145 - Psest_0888 0.89 -1.4 966,146 + Psest_0888 0.89 -0.7 966,270 - -0.3 966,270 - +0.7 966,414 - +0.5 966,428 - -1.2 966,430 - -0.6 966,430 - -0.9 966,430 - +0.8 966,430 - +0.5 966,430 - -3.3 966,430 - -0.3 966,432 + -1.5 966,433 - +1.8 966,433 - -0.1 966,433 - +0.2 966,433 - -0.1 966,492 + -0.3 966,492 + +2.5 966,493 - -1.5 966,528 + Psest_0889 0.12 -1.5 966,529 - Psest_0889 0.13 -2.2 966,529 - Psest_0889 0.13 -2.3 966,529 - Psest_0889 0.13 -2.5 966,529 - Psest_0889 0.13 -0.8 966,537 + Psest_0889 0.15 -0.1 966,537 + Psest_0889 0.15 -2.6 966,537 + Psest_0889 0.15 -2.2 966,537 + Psest_0889 0.15 +0.1 966,537 + Psest_0889 0.15 +0.2 966,537 + Psest_0889 0.15 -1.0 966,538 - Psest_0889 0.15 -1.6 966,538 - Psest_0889 0.15 +2.5 966,731 + Psest_0889 0.63 +1.9 966,792 + Psest_0889 0.78 -0.9 966,792 + Psest_0889 0.78 -2.3 966,793 - Psest_0889 0.79 +1.0 966,793 - Psest_0889 0.79 +1.4 966,881 + -0.6 966,881 + -1.2 966,882 - -1.9 966,882 - -0.8 966,882 - -1.1 966,882 - +1.1
Or see this region's nucleotide sequence