Experiment: Def Neomycin 0.0008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0083 and Psest_0084 overlap by 4 nucleotides Psest_0084 and Psest_0085 are separated by 134 nucleotides
Psest_0083: Psest_0083 - Uncharacterized conserved protein, at 84,675 to 85,082
_0083
Psest_0084: Psest_0084 - alpha-ketoglutarate TRAP transporter, 4TM/12TM components (from data) , at 85,079 to 87,103
_0084
Psest_0085: Psest_0085 - alpha-ketoglutarate TRAP transporter, solute receptor component (from data) , at 87,238 to 88,191
_0085
Position (kb)
85
86
87
88 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 84.120 kb on + strand at 84.120 kb on + strand at 84.121 kb on - strand at 84.121 kb on - strand at 84.121 kb on - strand at 84.121 kb on - strand at 84.121 kb on - strand at 84.121 kb on - strand at 84.221 kb on + strand at 84.222 kb on - strand at 84.499 kb on - strand at 84.591 kb on - strand at 84.591 kb on - strand at 84.591 kb on - strand at 84.605 kb on - strand at 84.707 kb on + strand at 84.707 kb on + strand at 84.707 kb on + strand at 84.800 kb on - strand, within Psest_0083 at 84.800 kb on - strand, within Psest_0083 at 84.863 kb on - strand, within Psest_0083 at 84.971 kb on - strand, within Psest_0083 at 85.241 kb on + strand at 85.241 kb on + strand at 85.241 kb on + strand at 85.241 kb on + strand at 85.241 kb on + strand at 85.242 kb on - strand at 85.242 kb on - strand at 85.242 kb on - strand at 85.242 kb on - strand at 85.242 kb on - strand at 85.242 kb on - strand at 85.242 kb on - strand at 85.242 kb on - strand at 85.302 kb on + strand, within Psest_0084 at 85.302 kb on + strand, within Psest_0084 at 85.302 kb on + strand, within Psest_0084 at 85.302 kb on + strand, within Psest_0084 at 85.302 kb on + strand, within Psest_0084 at 85.302 kb on + strand, within Psest_0084 at 85.302 kb on + strand, within Psest_0084 at 85.303 kb on - strand, within Psest_0084 at 85.303 kb on - strand, within Psest_0084 at 85.303 kb on - strand, within Psest_0084 at 85.303 kb on - strand, within Psest_0084 at 85.303 kb on - strand, within Psest_0084 at 85.303 kb on - strand, within Psest_0084 at 85.303 kb on - strand, within Psest_0084 at 85.303 kb on - strand, within Psest_0084 at 85.350 kb on + strand, within Psest_0084 at 85.350 kb on + strand, within Psest_0084 at 85.350 kb on + strand, within Psest_0084 at 85.350 kb on + strand, within Psest_0084 at 85.350 kb on + strand, within Psest_0084 at 85.351 kb on - strand, within Psest_0084 at 85.351 kb on - strand, within Psest_0084 at 85.351 kb on - strand, within Psest_0084 at 85.351 kb on - strand, within Psest_0084 at 85.497 kb on + strand, within Psest_0084 at 85.497 kb on + strand, within Psest_0084 at 85.497 kb on + strand, within Psest_0084 at 85.497 kb on + strand, within Psest_0084 at 85.497 kb on + strand, within Psest_0084 at 85.498 kb on - strand, within Psest_0084 at 85.498 kb on - strand, within Psest_0084 at 85.578 kb on + strand, within Psest_0084 at 85.578 kb on + strand, within Psest_0084 at 85.578 kb on + strand, within Psest_0084 at 85.578 kb on + strand, within Psest_0084 at 85.578 kb on + strand, within Psest_0084 at 85.578 kb on + strand, within Psest_0084 at 85.578 kb on + strand, within Psest_0084 at 85.578 kb on + strand, within Psest_0084 at 85.578 kb on + strand, within Psest_0084 at 85.579 kb on - strand, within Psest_0084 at 85.678 kb on - strand, within Psest_0084 at 85.844 kb on + strand, within Psest_0084 at 85.845 kb on - strand, within Psest_0084 at 86.190 kb on + strand, within Psest_0084 at 86.190 kb on + strand, within Psest_0084 at 86.190 kb on + strand, within Psest_0084 at 86.565 kb on + strand, within Psest_0084 at 86.565 kb on + strand, within Psest_0084 at 86.565 kb on + strand, within Psest_0084 at 86.565 kb on + strand, within Psest_0084 at 86.565 kb on + strand, within Psest_0084 at 86.565 kb on + strand, within Psest_0084 at 86.565 kb on + strand, within Psest_0084 at 86.565 kb on + strand, within Psest_0084 at 86.565 kb on + strand, within Psest_0084 at 86.565 kb on + strand, within Psest_0084 at 86.565 kb on + strand, within Psest_0084 at 86.565 kb on + strand, within Psest_0084 at 86.566 kb on - strand, within Psest_0084 at 86.566 kb on - strand, within Psest_0084 at 86.566 kb on - strand, within Psest_0084 at 86.566 kb on - strand, within Psest_0084 at 86.566 kb on - strand, within Psest_0084 at 86.566 kb on - strand, within Psest_0084 at 86.566 kb on - strand, within Psest_0084 at 86.566 kb on - strand, within Psest_0084 at 86.566 kb on - strand, within Psest_0084 at 86.566 kb on - strand, within Psest_0084 at 86.566 kb on - strand, within Psest_0084 at 86.566 kb on - strand, within Psest_0084 at 86.566 kb on - strand, within Psest_0084 at 86.566 kb on - strand, within Psest_0084 at 86.566 kb on - strand, within Psest_0084 at 86.566 kb on - strand, within Psest_0084 at 86.580 kb on + strand, within Psest_0084 at 86.580 kb on + strand, within Psest_0084 at 86.581 kb on - strand, within Psest_0084 at 86.581 kb on - strand, within Psest_0084 at 86.581 kb on - strand, within Psest_0084 at 86.581 kb on - strand, within Psest_0084 at 86.595 kb on + strand, within Psest_0084 at 86.618 kb on + strand, within Psest_0084 at 86.643 kb on + strand, within Psest_0084 at 86.643 kb on + strand, within Psest_0084 at 86.643 kb on + strand, within Psest_0084 at 86.644 kb on - strand, within Psest_0084 at 86.644 kb on - strand, within Psest_0084 at 86.644 kb on - strand, within Psest_0084 at 86.644 kb on - strand, within Psest_0084 at 86.646 kb on + strand, within Psest_0084 at 86.646 kb on + strand, within Psest_0084 at 86.676 kb on + strand, within Psest_0084 at 86.677 kb on - strand, within Psest_0084 at 86.841 kb on + strand at 86.841 kb on + strand, within Psest_0084 at 86.841 kb on + strand, within Psest_0084 at 86.841 kb on + strand, within Psest_0084 at 86.841 kb on + strand, within Psest_0084 at 86.842 kb on - strand, within Psest_0084 at 86.842 kb on - strand, within Psest_0084 at 86.842 kb on - strand, within Psest_0084 at 87.021 kb on + strand at 87.022 kb on - strand at 87.045 kb on + strand at 87.046 kb on - strand at 87.046 kb on - strand at 87.046 kb on - strand at 87.046 kb on - strand at 87.046 kb on - strand at 87.046 kb on - strand at 87.046 kb on - strand at 87.046 kb on - strand at 87.046 kb on - strand at 87.137 kb on + strand at 87.137 kb on + strand at 87.137 kb on + strand at 87.137 kb on + strand at 87.137 kb on + strand at 87.138 kb on - strand at 87.181 kb on + strand at 87.181 kb on + strand at 87.182 kb on - strand at 87.239 kb on + strand at 87.239 kb on + strand at 87.263 kb on + strand at 87.263 kb on + strand at 87.263 kb on + strand at 87.263 kb on + strand at 87.263 kb on + strand at 87.263 kb on + strand at 87.263 kb on + strand at 87.263 kb on + strand at 87.264 kb on - strand at 87.264 kb on - strand at 87.396 kb on - strand, within Psest_0085 at 87.479 kb on + strand, within Psest_0085 at 87.479 kb on + strand, within Psest_0085 at 87.479 kb on + strand, within Psest_0085 at 87.480 kb on - strand, within Psest_0085 at 87.480 kb on - strand, within Psest_0085 at 87.480 kb on - strand, within Psest_0085 at 87.510 kb on - strand, within Psest_0085 at 87.668 kb on + strand, within Psest_0085 at 87.668 kb on + strand, within Psest_0085 at 87.668 kb on + strand, within Psest_0085 at 87.668 kb on + strand, within Psest_0085 at 87.701 kb on + strand, within Psest_0085 at 87.701 kb on + strand, within Psest_0085 at 87.701 kb on + strand, within Psest_0085 at 87.701 kb on + strand, within Psest_0085 at 87.702 kb on - strand, within Psest_0085 at 87.702 kb on - strand, within Psest_0085 at 87.702 kb on - strand, within Psest_0085 at 87.839 kb on + strand, within Psest_0085 at 87.839 kb on + strand, within Psest_0085 at 87.839 kb on + strand, within Psest_0085 at 87.839 kb on + strand, within Psest_0085 at 87.839 kb on + strand, within Psest_0085 at 87.839 kb on + strand, within Psest_0085 at 87.839 kb on + strand, within Psest_0085 at 87.840 kb on - strand, within Psest_0085 at 87.840 kb on - strand, within Psest_0085 at 87.840 kb on - strand, within Psest_0085 at 87.840 kb on - strand, within Psest_0085 at 87.840 kb on - strand, within Psest_0085 at 87.840 kb on - strand, within Psest_0085 at 87.848 kb on + strand, within Psest_0085 at 87.848 kb on + strand, within Psest_0085 at 87.848 kb on + strand, within Psest_0085 at 87.848 kb on + strand, within Psest_0085 at 87.848 kb on + strand, within Psest_0085 at 87.848 kb on + strand, within Psest_0085 at 87.849 kb on - strand, within Psest_0085 at 87.849 kb on - strand, within Psest_0085 at 87.849 kb on - strand, within Psest_0085 at 87.849 kb on - strand, within Psest_0085 at 87.849 kb on - strand, within Psest_0085 at 87.849 kb on - strand, within Psest_0085 at 87.849 kb on - strand, within Psest_0085 at 87.849 kb on - strand, within Psest_0085 at 87.849 kb on - strand, within Psest_0085 at 87.988 kb on + strand, within Psest_0085 at 88.040 kb on + strand, within Psest_0085 at 88.040 kb on + strand, within Psest_0085 at 88.040 kb on + strand, within Psest_0085 at 88.040 kb on + strand, within Psest_0085 at 88.040 kb on + strand, within Psest_0085 at 88.040 kb on + strand, within Psest_0085 at 88.041 kb on - strand, within Psest_0085 at 88.041 kb on - strand, within Psest_0085 at 88.073 kb on + strand, within Psest_0085 at 88.073 kb on + strand, within Psest_0085 at 88.074 kb on - strand, within Psest_0085 at 88.074 kb on - strand, within Psest_0085 at 88.074 kb on - strand, within Psest_0085
Per-strain Table
Position Strand Gene LocusTag Fraction Def Neomycin 0.0008 mg/ml remove 84,120 + +1.7 84,120 + +1.3 84,121 - +0.9 84,121 - +1.2 84,121 - +0.7 84,121 - +0.8 84,121 - +0.5 84,121 - -0.8 84,221 + -1.6 84,222 - +0.7 84,499 - -0.6 84,591 - -0.2 84,591 - +0.1 84,591 - +1.9 84,605 - -0.3 84,707 + +0.5 84,707 + +1.3 84,707 + +2.0 84,800 - Psest_0083 0.31 +0.3 84,800 - Psest_0083 0.31 -0.0 84,863 - Psest_0083 0.46 -2.0 84,971 - Psest_0083 0.73 +0.6 85,241 + +1.4 85,241 + -1.6 85,241 + -0.8 85,241 + -1.5 85,241 + +0.1 85,242 - -2.0 85,242 - -0.5 85,242 - +2.5 85,242 - -1.3 85,242 - -1.8 85,242 - -0.0 85,242 - +1.5 85,242 - -2.0 85,302 + Psest_0084 0.11 -1.9 85,302 + Psest_0084 0.11 +1.7 85,302 + Psest_0084 0.11 +0.7 85,302 + Psest_0084 0.11 -0.6 85,302 + Psest_0084 0.11 +0.9 85,302 + Psest_0084 0.11 +0.5 85,302 + Psest_0084 0.11 +0.4 85,303 - Psest_0084 0.11 +1.3 85,303 - Psest_0084 0.11 -1.3 85,303 - Psest_0084 0.11 +2.0 85,303 - Psest_0084 0.11 -1.6 85,303 - Psest_0084 0.11 +2.7 85,303 - Psest_0084 0.11 -0.9 85,303 - Psest_0084 0.11 -1.3 85,303 - Psest_0084 0.11 -1.2 85,350 + Psest_0084 0.13 +1.2 85,350 + Psest_0084 0.13 +1.9 85,350 + Psest_0084 0.13 -0.5 85,350 + Psest_0084 0.13 -1.5 85,350 + Psest_0084 0.13 -1.1 85,351 - Psest_0084 0.13 -1.1 85,351 - Psest_0084 0.13 -0.5 85,351 - Psest_0084 0.13 -0.3 85,351 - Psest_0084 0.13 -3.5 85,497 + Psest_0084 0.21 -1.3 85,497 + Psest_0084 0.21 +1.3 85,497 + Psest_0084 0.21 -1.2 85,497 + Psest_0084 0.21 +0.5 85,497 + Psest_0084 0.21 +0.1 85,498 - Psest_0084 0.21 -0.1 85,498 - Psest_0084 0.21 -0.6 85,578 + Psest_0084 0.25 -0.0 85,578 + Psest_0084 0.25 +1.3 85,578 + Psest_0084 0.25 -0.9 85,578 + Psest_0084 0.25 +0.2 85,578 + Psest_0084 0.25 -0.9 85,578 + Psest_0084 0.25 +0.4 85,578 + Psest_0084 0.25 -0.6 85,578 + Psest_0084 0.25 +0.7 85,578 + Psest_0084 0.25 -1.9 85,579 - Psest_0084 0.25 -1.1 85,678 - Psest_0084 0.30 +0.7 85,844 + Psest_0084 0.38 -1.9 85,845 - Psest_0084 0.38 +0.1 86,190 + Psest_0084 0.55 -1.5 86,190 + Psest_0084 0.55 -0.3 86,190 + Psest_0084 0.55 +1.3 86,565 + Psest_0084 0.73 -0.6 86,565 + Psest_0084 0.73 +0.3 86,565 + Psest_0084 0.73 -0.3 86,565 + Psest_0084 0.73 -1.3 86,565 + Psest_0084 0.73 +0.5 86,565 + Psest_0084 0.73 -1.1 86,565 + Psest_0084 0.73 +0.1 86,565 + Psest_0084 0.73 -1.5 86,565 + Psest_0084 0.73 -0.3 86,565 + Psest_0084 0.73 +0.4 86,565 + Psest_0084 0.73 +1.0 86,565 + Psest_0084 0.73 +0.4 86,566 - Psest_0084 0.73 +1.1 86,566 - Psest_0084 0.73 +2.3 86,566 - Psest_0084 0.73 +1.0 86,566 - Psest_0084 0.73 +1.0 86,566 - Psest_0084 0.73 +0.2 86,566 - Psest_0084 0.73 +1.7 86,566 - Psest_0084 0.73 +0.7 86,566 - Psest_0084 0.73 +2.0 86,566 - Psest_0084 0.73 -1.6 86,566 - Psest_0084 0.73 +1.1 86,566 - Psest_0084 0.73 -1.0 86,566 - Psest_0084 0.73 -0.6 86,566 - Psest_0084 0.73 +0.5 86,566 - Psest_0084 0.73 -0.1 86,566 - Psest_0084 0.73 -0.3 86,566 - Psest_0084 0.73 +2.0 86,580 + Psest_0084 0.74 -1.4 86,580 + Psest_0084 0.74 +1.7 86,581 - Psest_0084 0.74 -0.9 86,581 - Psest_0084 0.74 +0.2 86,581 - Psest_0084 0.74 +0.7 86,581 - Psest_0084 0.74 -0.5 86,595 + Psest_0084 0.75 -0.4 86,618 + Psest_0084 0.76 -0.3 86,643 + Psest_0084 0.77 +1.0 86,643 + Psest_0084 0.77 -2.5 86,643 + Psest_0084 0.77 +0.3 86,644 - Psest_0084 0.77 -1.3 86,644 - Psest_0084 0.77 -1.0 86,644 - Psest_0084 0.77 +1.0 86,644 - Psest_0084 0.77 +0.0 86,646 + Psest_0084 0.77 +0.7 86,646 + Psest_0084 0.77 -0.6 86,676 + Psest_0084 0.79 +1.0 86,677 - Psest_0084 0.79 -2.1 86,841 + +3.3 86,841 + Psest_0084 0.87 +0.3 86,841 + Psest_0084 0.87 -0.8 86,841 + Psest_0084 0.87 -1.2 86,841 + Psest_0084 0.87 +0.3 86,842 - Psest_0084 0.87 -1.0 86,842 - Psest_0084 0.87 +0.8 86,842 - Psest_0084 0.87 -0.0 87,021 + +0.7 87,022 - +2.8 87,045 + +1.6 87,046 - +4.3 87,046 - -0.7 87,046 - +2.0 87,046 - +0.0 87,046 - +0.1 87,046 - -0.8 87,046 - +2.0 87,046 - -0.6 87,046 - +0.1 87,137 + -0.9 87,137 + -1.9 87,137 + -1.0 87,137 + -1.2 87,137 + -1.4 87,138 - +1.0 87,181 + +0.6 87,181 + +0.7 87,182 - +0.4 87,239 + -0.2 87,239 + +2.5 87,263 + +1.7 87,263 + -1.1 87,263 + +0.2 87,263 + -0.4 87,263 + +0.1 87,263 + -0.3 87,263 + +0.6 87,263 + +1.6 87,264 - +0.6 87,264 - -1.3 87,396 - Psest_0085 0.17 -0.6 87,479 + Psest_0085 0.25 +0.7 87,479 + Psest_0085 0.25 -0.0 87,479 + Psest_0085 0.25 -0.3 87,480 - Psest_0085 0.25 +0.7 87,480 - Psest_0085 0.25 -1.6 87,480 - Psest_0085 0.25 -0.2 87,510 - Psest_0085 0.29 +0.5 87,668 + Psest_0085 0.45 +1.0 87,668 + Psest_0085 0.45 -0.5 87,668 + Psest_0085 0.45 +1.1 87,668 + Psest_0085 0.45 +0.1 87,701 + Psest_0085 0.49 +1.2 87,701 + Psest_0085 0.49 +1.5 87,701 + Psest_0085 0.49 -1.5 87,701 + Psest_0085 0.49 +3.4 87,702 - Psest_0085 0.49 +0.1 87,702 - Psest_0085 0.49 +1.8 87,702 - Psest_0085 0.49 -2.0 87,839 + Psest_0085 0.63 -0.5 87,839 + Psest_0085 0.63 +1.7 87,839 + Psest_0085 0.63 +1.2 87,839 + Psest_0085 0.63 +1.0 87,839 + Psest_0085 0.63 +0.3 87,839 + Psest_0085 0.63 +0.4 87,839 + Psest_0085 0.63 +0.8 87,840 - Psest_0085 0.63 -0.5 87,840 - Psest_0085 0.63 +1.5 87,840 - Psest_0085 0.63 -0.3 87,840 - Psest_0085 0.63 -1.9 87,840 - Psest_0085 0.63 -0.5 87,840 - Psest_0085 0.63 +0.6 87,848 + Psest_0085 0.64 +1.7 87,848 + Psest_0085 0.64 -0.1 87,848 + Psest_0085 0.64 -0.4 87,848 + Psest_0085 0.64 -0.9 87,848 + Psest_0085 0.64 -0.6 87,848 + Psest_0085 0.64 -0.3 87,849 - Psest_0085 0.64 -0.7 87,849 - Psest_0085 0.64 -1.7 87,849 - Psest_0085 0.64 +0.3 87,849 - Psest_0085 0.64 +1.0 87,849 - Psest_0085 0.64 +0.1 87,849 - Psest_0085 0.64 +1.2 87,849 - Psest_0085 0.64 +2.0 87,849 - Psest_0085 0.64 -0.0 87,849 - Psest_0085 0.64 -1.8 87,988 + Psest_0085 0.79 -1.6 88,040 + Psest_0085 0.84 +0.2 88,040 + Psest_0085 0.84 -0.6 88,040 + Psest_0085 0.84 -2.0 88,040 + Psest_0085 0.84 -0.1 88,040 + Psest_0085 0.84 +2.1 88,040 + Psest_0085 0.84 -1.1 88,041 - Psest_0085 0.84 +0.7 88,041 - Psest_0085 0.84 -2.5 88,073 + Psest_0085 0.88 +0.6 88,073 + Psest_0085 0.88 +0.9 88,074 - Psest_0085 0.88 -0.3 88,074 - Psest_0085 0.88 -0.3 88,074 - Psest_0085 0.88 -1.0
Or see this region's nucleotide sequence