Experiment: Def Neomycin 0.0008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0752 and Psest_0753 are separated by 267 nucleotides Psest_0753 and Psest_0754 are separated by 88 nucleotides Psest_0754 and Psest_0755 are separated by 92 nucleotides
Psest_0752: Psest_0752 - Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain, at 823,433 to 824,422
_0752
Psest_0753: Psest_0753 - NADH:flavin oxidoreductases, Old Yellow Enzyme family, at 824,690 to 825,814
_0753
Psest_0754: Psest_0754 - Predicted transcriptional regulators, at 825,903 to 826,208
_0754
Psest_0755: Psest_0755 - Bacterial regulatory helix-turn-helix proteins, AraC family., at 826,301 to 827,098
_0755
Position (kb)
824
825
826 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 823.950 kb on + strand, within Psest_0752 at 823.950 kb on + strand, within Psest_0752 at 823.950 kb on + strand, within Psest_0752 at 823.950 kb on + strand, within Psest_0752 at 823.950 kb on + strand, within Psest_0752 at 823.950 kb on + strand, within Psest_0752 at 823.951 kb on - strand, within Psest_0752 at 823.951 kb on - strand, within Psest_0752 at 823.951 kb on - strand, within Psest_0752 at 823.951 kb on - strand, within Psest_0752 at 823.951 kb on - strand, within Psest_0752 at 823.990 kb on - strand, within Psest_0752 at 824.296 kb on - strand, within Psest_0752 at 824.296 kb on - strand, within Psest_0752 at 824.388 kb on + strand at 824.388 kb on + strand at 824.512 kb on + strand at 824.512 kb on + strand at 824.513 kb on - strand at 824.513 kb on - strand at 824.513 kb on - strand at 824.513 kb on - strand at 824.513 kb on - strand at 824.513 kb on - strand at 824.699 kb on - strand at 824.710 kb on + strand at 824.710 kb on + strand at 824.710 kb on + strand at 824.711 kb on - strand at 824.711 kb on - strand at 824.739 kb on + strand at 824.740 kb on - strand at 824.743 kb on + strand at 824.744 kb on - strand at 824.744 kb on - strand at 824.822 kb on + strand, within Psest_0753 at 824.822 kb on + strand, within Psest_0753 at 824.822 kb on + strand, within Psest_0753 at 824.822 kb on + strand, within Psest_0753 at 824.823 kb on - strand, within Psest_0753 at 824.823 kb on - strand, within Psest_0753 at 824.826 kb on - strand, within Psest_0753 at 824.826 kb on - strand, within Psest_0753 at 824.894 kb on + strand, within Psest_0753 at 824.894 kb on + strand, within Psest_0753 at 824.895 kb on - strand, within Psest_0753 at 825.182 kb on + strand, within Psest_0753 at 825.182 kb on + strand, within Psest_0753 at 825.183 kb on - strand, within Psest_0753 at 825.302 kb on + strand, within Psest_0753 at 825.334 kb on + strand, within Psest_0753 at 825.334 kb on + strand, within Psest_0753 at 825.434 kb on + strand, within Psest_0753 at 825.434 kb on + strand, within Psest_0753 at 825.470 kb on + strand, within Psest_0753 at 825.470 kb on + strand, within Psest_0753 at 825.470 kb on + strand, within Psest_0753 at 825.470 kb on + strand, within Psest_0753 at 825.470 kb on + strand, within Psest_0753 at 825.471 kb on - strand, within Psest_0753 at 825.471 kb on - strand, within Psest_0753 at 825.471 kb on - strand, within Psest_0753 at 825.471 kb on - strand, within Psest_0753 at 825.512 kb on + strand, within Psest_0753 at 825.516 kb on + strand, within Psest_0753 at 825.516 kb on + strand, within Psest_0753 at 825.516 kb on + strand, within Psest_0753 at 825.517 kb on - strand, within Psest_0753 at 825.517 kb on - strand, within Psest_0753 at 825.608 kb on + strand, within Psest_0753 at 825.608 kb on + strand, within Psest_0753 at 825.608 kb on + strand, within Psest_0753 at 825.609 kb on - strand, within Psest_0753 at 825.609 kb on - strand, within Psest_0753 at 825.620 kb on + strand, within Psest_0753 at 825.620 kb on + strand, within Psest_0753 at 825.620 kb on + strand, within Psest_0753 at 825.621 kb on - strand, within Psest_0753 at 825.621 kb on - strand, within Psest_0753 at 825.621 kb on - strand, within Psest_0753 at 825.621 kb on - strand, within Psest_0753 at 825.621 kb on - strand, within Psest_0753 at 825.736 kb on + strand at 825.765 kb on - strand at 825.785 kb on + strand at 825.786 kb on - strand at 825.786 kb on - strand at 825.870 kb on + strand at 825.902 kb on + strand at 825.902 kb on + strand at 825.910 kb on + strand at 825.910 kb on + strand at 825.910 kb on + strand at 825.910 kb on + strand at 825.910 kb on + strand at 825.910 kb on + strand at 825.910 kb on + strand at 825.910 kb on + strand at 825.910 kb on + strand at 825.910 kb on + strand at 825.911 kb on - strand at 825.911 kb on - strand at 825.911 kb on - strand at 825.911 kb on - strand at 826.086 kb on + strand, within Psest_0754 at 826.086 kb on + strand, within Psest_0754 at 826.146 kb on + strand, within Psest_0754 at 826.146 kb on + strand, within Psest_0754 at 826.146 kb on + strand, within Psest_0754 at 826.146 kb on + strand, within Psest_0754 at 826.147 kb on - strand, within Psest_0754 at 826.147 kb on - strand, within Psest_0754 at 826.147 kb on - strand, within Psest_0754 at 826.147 kb on - strand, within Psest_0754 at 826.147 kb on - strand, within Psest_0754 at 826.149 kb on + strand, within Psest_0754 at 826.149 kb on + strand, within Psest_0754 at 826.149 kb on + strand, within Psest_0754 at 826.149 kb on + strand, within Psest_0754 at 826.149 kb on + strand, within Psest_0754 at 826.149 kb on + strand, within Psest_0754 at 826.149 kb on + strand, within Psest_0754 at 826.150 kb on - strand, within Psest_0754 at 826.150 kb on - strand, within Psest_0754 at 826.150 kb on - strand, within Psest_0754 at 826.150 kb on - strand, within Psest_0754 at 826.150 kb on - strand, within Psest_0754 at 826.150 kb on - strand, within Psest_0754 at 826.150 kb on - strand, within Psest_0754 at 826.150 kb on - strand, within Psest_0754 at 826.493 kb on + strand, within Psest_0755 at 826.493 kb on + strand, within Psest_0755 at 826.493 kb on + strand, within Psest_0755 at 826.493 kb on + strand, within Psest_0755 at 826.494 kb on - strand, within Psest_0755 at 826.494 kb on - strand, within Psest_0755 at 826.494 kb on - strand, within Psest_0755 at 826.494 kb on - strand, within Psest_0755 at 826.494 kb on - strand, within Psest_0755 at 826.494 kb on - strand, within Psest_0755 at 826.494 kb on - strand, within Psest_0755 at 826.494 kb on - strand, within Psest_0755 at 826.494 kb on - strand, within Psest_0755 at 826.598 kb on + strand, within Psest_0755 at 826.598 kb on + strand, within Psest_0755 at 826.598 kb on + strand, within Psest_0755 at 826.599 kb on - strand, within Psest_0755 at 826.599 kb on - strand, within Psest_0755 at 826.744 kb on + strand, within Psest_0755 at 826.745 kb on - strand, within Psest_0755 at 826.745 kb on - strand, within Psest_0755 at 826.745 kb on - strand, within Psest_0755
Per-strain Table
Position Strand Gene LocusTag Fraction Def Neomycin 0.0008 mg/ml remove 823,950 + Psest_0752 0.52 -0.5 823,950 + Psest_0752 0.52 -0.2 823,950 + Psest_0752 0.52 -0.9 823,950 + Psest_0752 0.52 +0.3 823,950 + Psest_0752 0.52 +1.3 823,950 + Psest_0752 0.52 +1.1 823,951 - Psest_0752 0.52 +1.0 823,951 - Psest_0752 0.52 +1.8 823,951 - Psest_0752 0.52 +0.4 823,951 - Psest_0752 0.52 -0.6 823,951 - Psest_0752 0.52 +0.7 823,990 - Psest_0752 0.56 +0.3 824,296 - Psest_0752 0.87 -1.5 824,296 - Psest_0752 0.87 -0.1 824,388 + +2.4 824,388 + -0.1 824,512 + -1.9 824,512 + -2.2 824,513 - -0.3 824,513 - +0.6 824,513 - +1.7 824,513 - -0.6 824,513 - +0.4 824,513 - -0.8 824,699 - +0.3 824,710 + -0.1 824,710 + -1.6 824,710 + -0.5 824,711 - +1.9 824,711 - -1.3 824,739 + +0.7 824,740 - +1.7 824,743 + -1.8 824,744 - +2.0 824,744 - +1.4 824,822 + Psest_0753 0.12 +0.8 824,822 + Psest_0753 0.12 +0.4 824,822 + Psest_0753 0.12 +1.1 824,822 + Psest_0753 0.12 +0.5 824,823 - Psest_0753 0.12 -0.1 824,823 - Psest_0753 0.12 +0.5 824,826 - Psest_0753 0.12 +1.1 824,826 - Psest_0753 0.12 +0.1 824,894 + Psest_0753 0.18 +0.2 824,894 + Psest_0753 0.18 +0.7 824,895 - Psest_0753 0.18 -1.5 825,182 + Psest_0753 0.44 +0.7 825,182 + Psest_0753 0.44 -1.7 825,183 - Psest_0753 0.44 -1.3 825,302 + Psest_0753 0.54 +2.5 825,334 + Psest_0753 0.57 +0.1 825,334 + Psest_0753 0.57 -1.2 825,434 + Psest_0753 0.66 +0.0 825,434 + Psest_0753 0.66 +0.6 825,470 + Psest_0753 0.69 +1.5 825,470 + Psest_0753 0.69 -0.9 825,470 + Psest_0753 0.69 -0.1 825,470 + Psest_0753 0.69 +1.0 825,470 + Psest_0753 0.69 +0.2 825,471 - Psest_0753 0.69 -0.2 825,471 - Psest_0753 0.69 +1.5 825,471 - Psest_0753 0.69 -1.9 825,471 - Psest_0753 0.69 +1.2 825,512 + Psest_0753 0.73 -0.6 825,516 + Psest_0753 0.73 -0.3 825,516 + Psest_0753 0.73 +0.7 825,516 + Psest_0753 0.73 +1.6 825,517 - Psest_0753 0.74 -2.2 825,517 - Psest_0753 0.74 -2.0 825,608 + Psest_0753 0.82 -1.1 825,608 + Psest_0753 0.82 +0.4 825,608 + Psest_0753 0.82 -0.2 825,609 - Psest_0753 0.82 +1.3 825,609 - Psest_0753 0.82 +1.7 825,620 + Psest_0753 0.83 +0.0 825,620 + Psest_0753 0.83 +1.7 825,620 + Psest_0753 0.83 +1.9 825,621 - Psest_0753 0.83 -0.5 825,621 - Psest_0753 0.83 +0.7 825,621 - Psest_0753 0.83 +1.1 825,621 - Psest_0753 0.83 +0.9 825,621 - Psest_0753 0.83 +0.3 825,736 + -2.1 825,765 - +0.5 825,785 + -2.0 825,786 - -1.6 825,786 - -0.9 825,870 + -1.5 825,902 + -0.9 825,902 + +1.1 825,910 + +0.1 825,910 + +0.7 825,910 + +1.2 825,910 + +2.0 825,910 + +1.5 825,910 + -0.6 825,910 + -2.6 825,910 + -2.1 825,910 + +0.3 825,910 + -0.2 825,911 - -1.0 825,911 - -1.6 825,911 - -0.7 825,911 - +2.5 826,086 + Psest_0754 0.60 +0.3 826,086 + Psest_0754 0.60 +1.7 826,146 + Psest_0754 0.79 +1.2 826,146 + Psest_0754 0.79 -0.6 826,146 + Psest_0754 0.79 -2.3 826,146 + Psest_0754 0.79 -2.6 826,147 - Psest_0754 0.80 -0.0 826,147 - Psest_0754 0.80 -0.5 826,147 - Psest_0754 0.80 +0.4 826,147 - Psest_0754 0.80 -0.3 826,147 - Psest_0754 0.80 +0.6 826,149 + Psest_0754 0.80 -0.6 826,149 + Psest_0754 0.80 -0.6 826,149 + Psest_0754 0.80 +1.0 826,149 + Psest_0754 0.80 -0.1 826,149 + Psest_0754 0.80 -0.3 826,149 + Psest_0754 0.80 -0.6 826,149 + Psest_0754 0.80 -1.3 826,150 - Psest_0754 0.81 +1.4 826,150 - Psest_0754 0.81 +1.4 826,150 - Psest_0754 0.81 +1.6 826,150 - Psest_0754 0.81 -0.6 826,150 - Psest_0754 0.81 +1.0 826,150 - Psest_0754 0.81 +1.6 826,150 - Psest_0754 0.81 +0.4 826,150 - Psest_0754 0.81 -0.3 826,493 + Psest_0755 0.24 -1.3 826,493 + Psest_0755 0.24 -0.5 826,493 + Psest_0755 0.24 -0.1 826,493 + Psest_0755 0.24 +0.1 826,494 - Psest_0755 0.24 +1.7 826,494 - Psest_0755 0.24 -0.4 826,494 - Psest_0755 0.24 +1.1 826,494 - Psest_0755 0.24 +0.4 826,494 - Psest_0755 0.24 -1.1 826,494 - Psest_0755 0.24 +0.8 826,494 - Psest_0755 0.24 +0.8 826,494 - Psest_0755 0.24 -0.1 826,494 - Psest_0755 0.24 -0.3 826,598 + Psest_0755 0.37 -0.4 826,598 + Psest_0755 0.37 +0.2 826,598 + Psest_0755 0.37 -0.9 826,599 - Psest_0755 0.37 -1.3 826,599 - Psest_0755 0.37 +0.6 826,744 + Psest_0755 0.56 -0.7 826,745 - Psest_0755 0.56 -1.3 826,745 - Psest_0755 0.56 +0.1 826,745 - Psest_0755 0.56 +0.7
Or see this region's nucleotide sequence