Experiment: Def Neomycin 0.0008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_4246 and Psest_4247 overlap by 1 nucleotides Psest_4247 and Psest_4248 overlap by 4 nucleotides Psest_4248 and Psest_4249 are separated by 20 nucleotides
Psest_4246: Psest_4246 - type VI secretion protein IcmF, at 4,428,515 to 4,432,054
_4246
Psest_4247: Psest_4247 - Serine/threonine protein phosphatase, at 4,432,054 to 4,432,785
_4247
Psest_4248: Psest_4248 - Serine/threonine protein kinase, at 4,432,782 to 4,433,753
_4248
Psest_4249: Psest_4249 - Rhs element Vgr protein, at 4,433,774 to 4,435,819
_4249
Position (kb)
4432
4433
4434 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4431.974 kb on + strand at 4431.975 kb on - strand at 4431.975 kb on - strand at 4431.975 kb on - strand at 4431.975 kb on - strand at 4431.975 kb on - strand at 4431.975 kb on - strand at 4431.975 kb on - strand at 4432.239 kb on - strand, within Psest_4247 at 4432.429 kb on + strand, within Psest_4247 at 4432.430 kb on - strand, within Psest_4247 at 4432.430 kb on - strand, within Psest_4247 at 4432.453 kb on + strand, within Psest_4247 at 4432.649 kb on - strand, within Psest_4247 at 4432.649 kb on - strand, within Psest_4247 at 4432.812 kb on + strand at 4432.812 kb on + strand at 4432.812 kb on + strand at 4432.812 kb on + strand at 4432.813 kb on - strand at 4432.813 kb on - strand at 4432.888 kb on + strand, within Psest_4248 at 4432.995 kb on + strand, within Psest_4248 at 4432.995 kb on + strand, within Psest_4248 at 4432.996 kb on - strand, within Psest_4248 at 4432.996 kb on - strand, within Psest_4248 at 4432.996 kb on - strand, within Psest_4248 at 4432.996 kb on - strand, within Psest_4248 at 4433.059 kb on - strand, within Psest_4248 at 4433.059 kb on - strand, within Psest_4248 at 4433.068 kb on - strand, within Psest_4248 at 4433.113 kb on - strand, within Psest_4248 at 4433.113 kb on - strand, within Psest_4248 at 4433.113 kb on - strand, within Psest_4248 at 4433.113 kb on - strand, within Psest_4248 at 4433.113 kb on - strand, within Psest_4248 at 4433.206 kb on + strand, within Psest_4248 at 4433.207 kb on - strand, within Psest_4248 at 4433.268 kb on + strand, within Psest_4248 at 4433.268 kb on + strand, within Psest_4248 at 4433.268 kb on + strand, within Psest_4248 at 4433.268 kb on + strand, within Psest_4248 at 4433.268 kb on + strand, within Psest_4248 at 4433.269 kb on - strand, within Psest_4248 at 4433.269 kb on - strand, within Psest_4248 at 4433.287 kb on + strand, within Psest_4248 at 4433.288 kb on - strand, within Psest_4248 at 4433.288 kb on - strand, within Psest_4248 at 4433.437 kb on - strand, within Psest_4248 at 4433.490 kb on + strand, within Psest_4248 at 4433.490 kb on + strand, within Psest_4248 at 4433.490 kb on + strand, within Psest_4248 at 4433.490 kb on + strand, within Psest_4248 at 4433.491 kb on - strand, within Psest_4248 at 4433.491 kb on - strand, within Psest_4248 at 4433.491 kb on - strand, within Psest_4248 at 4433.491 kb on - strand, within Psest_4248 at 4433.511 kb on + strand, within Psest_4248 at 4433.512 kb on - strand, within Psest_4248 at 4433.512 kb on - strand, within Psest_4248 at 4433.512 kb on - strand, within Psest_4248 at 4433.512 kb on - strand, within Psest_4248 at 4433.942 kb on - strand at 4433.942 kb on - strand at 4433.982 kb on + strand, within Psest_4249 at 4433.982 kb on + strand, within Psest_4249 at 4433.982 kb on + strand, within Psest_4249 at 4433.982 kb on + strand, within Psest_4249 at 4433.982 kb on + strand, within Psest_4249 at 4433.982 kb on + strand, within Psest_4249 at 4433.983 kb on - strand, within Psest_4249 at 4433.983 kb on - strand, within Psest_4249 at 4433.983 kb on - strand, within Psest_4249 at 4433.983 kb on - strand, within Psest_4249 at 4433.983 kb on - strand, within Psest_4249 at 4434.096 kb on + strand, within Psest_4249 at 4434.097 kb on - strand, within Psest_4249 at 4434.210 kb on - strand, within Psest_4249 at 4434.222 kb on - strand, within Psest_4249 at 4434.271 kb on + strand, within Psest_4249 at 4434.271 kb on + strand, within Psest_4249 at 4434.272 kb on - strand, within Psest_4249 at 4434.278 kb on + strand, within Psest_4249 at 4434.461 kb on + strand, within Psest_4249 at 4434.462 kb on - strand, within Psest_4249 at 4434.552 kb on - strand, within Psest_4249
Per-strain Table
Position Strand Gene LocusTag Fraction Def Neomycin 0.0008 mg/ml remove 4,431,974 + -0.4 4,431,975 - +0.0 4,431,975 - +2.6 4,431,975 - +2.9 4,431,975 - -0.4 4,431,975 - -0.5 4,431,975 - -0.4 4,431,975 - -0.4 4,432,239 - Psest_4247 0.25 +0.6 4,432,429 + Psest_4247 0.51 -0.0 4,432,430 - Psest_4247 0.51 +0.3 4,432,430 - Psest_4247 0.51 +1.9 4,432,453 + Psest_4247 0.55 +0.0 4,432,649 - Psest_4247 0.81 +0.0 4,432,649 - Psest_4247 0.81 +0.9 4,432,812 + -1.3 4,432,812 + -0.6 4,432,812 + +2.1 4,432,812 + +0.7 4,432,813 - +0.6 4,432,813 - +0.4 4,432,888 + Psest_4248 0.11 -0.8 4,432,995 + Psest_4248 0.22 -1.8 4,432,995 + Psest_4248 0.22 +0.7 4,432,996 - Psest_4248 0.22 +0.2 4,432,996 - Psest_4248 0.22 -0.2 4,432,996 - Psest_4248 0.22 +2.0 4,432,996 - Psest_4248 0.22 -2.3 4,433,059 - Psest_4248 0.28 +2.6 4,433,059 - Psest_4248 0.28 -1.1 4,433,068 - Psest_4248 0.29 +0.2 4,433,113 - Psest_4248 0.34 +1.6 4,433,113 - Psest_4248 0.34 -0.7 4,433,113 - Psest_4248 0.34 +2.0 4,433,113 - Psest_4248 0.34 -1.8 4,433,113 - Psest_4248 0.34 +0.3 4,433,206 + Psest_4248 0.44 +1.3 4,433,207 - Psest_4248 0.44 +0.0 4,433,268 + Psest_4248 0.50 +2.4 4,433,268 + Psest_4248 0.50 -1.0 4,433,268 + Psest_4248 0.50 -0.5 4,433,268 + Psest_4248 0.50 +1.2 4,433,268 + Psest_4248 0.50 +0.4 4,433,269 - Psest_4248 0.50 +0.3 4,433,269 - Psest_4248 0.50 +1.9 4,433,287 + Psest_4248 0.52 +1.2 4,433,288 - Psest_4248 0.52 +1.6 4,433,288 - Psest_4248 0.52 -1.7 4,433,437 - Psest_4248 0.67 +2.6 4,433,490 + Psest_4248 0.73 +0.1 4,433,490 + Psest_4248 0.73 +0.4 4,433,490 + Psest_4248 0.73 +0.6 4,433,490 + Psest_4248 0.73 -0.7 4,433,491 - Psest_4248 0.73 -1.2 4,433,491 - Psest_4248 0.73 -0.8 4,433,491 - Psest_4248 0.73 +1.2 4,433,491 - Psest_4248 0.73 +1.7 4,433,511 + Psest_4248 0.75 -1.2 4,433,512 - Psest_4248 0.75 +2.9 4,433,512 - Psest_4248 0.75 +2.5 4,433,512 - Psest_4248 0.75 +1.3 4,433,512 - Psest_4248 0.75 +0.2 4,433,942 - +0.0 4,433,942 - +1.9 4,433,982 + Psest_4249 0.10 -1.6 4,433,982 + Psest_4249 0.10 -2.5 4,433,982 + Psest_4249 0.10 -1.4 4,433,982 + Psest_4249 0.10 -2.0 4,433,982 + Psest_4249 0.10 +1.5 4,433,982 + Psest_4249 0.10 +0.5 4,433,983 - Psest_4249 0.10 +1.0 4,433,983 - Psest_4249 0.10 -0.7 4,433,983 - Psest_4249 0.10 +0.6 4,433,983 - Psest_4249 0.10 +0.3 4,433,983 - Psest_4249 0.10 +1.6 4,434,096 + Psest_4249 0.16 -0.7 4,434,097 - Psest_4249 0.16 +0.6 4,434,210 - Psest_4249 0.21 -2.0 4,434,222 - Psest_4249 0.22 +0.8 4,434,271 + Psest_4249 0.24 +0.0 4,434,271 + Psest_4249 0.24 +0.6 4,434,272 - Psest_4249 0.24 +0.9 4,434,278 + Psest_4249 0.25 -0.9 4,434,461 + Psest_4249 0.34 -0.7 4,434,462 - Psest_4249 0.34 +0.0 4,434,552 - Psest_4249 0.38 -2.1
Or see this region's nucleotide sequence