Experiment: Def Neomycin 0.0008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0039 and Psest_0040 are separated by 248 nucleotides Psest_0040 and Psest_0041 are separated by 184 nucleotides Psest_0041 and Psest_0042 are separated by 118 nucleotides
Psest_0039: Psest_0039 - phosphoglucomutase, alpha-D-glucose phosphate-specific, at 41,591 to 43,255
_0039
Psest_0040: Psest_0040 - Protein of unknown function (DUF2934)., at 43,504 to 43,701
_0040
Psest_0041: Psest_0041 - Uncharacterized protein conserved in bacteria, at 43,886 to 45,367
_0041
Psest_0042: Psest_0042 - Transcriptional regulator, at 45,486 to 46,388
_0042
Position (kb) 43
44
45
46 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 43.176 kb on + strand at 43.176 kb on + strand at 43.176 kb on + strand at 43.177 kb on - strand at 43.177 kb on - strand at 43.177 kb on - strand at 43.177 kb on - strand at 43.177 kb on - strand at 43.177 kb on - strand at 43.337 kb on + strand at 43.338 kb on - strand at 43.347 kb on + strand at 43.347 kb on + strand at 43.348 kb on - strand at 43.537 kb on + strand, within Psest_0040 at 43.538 kb on - strand, within Psest_0040 at 43.621 kb on + strand, within Psest_0040 at 43.621 kb on + strand, within Psest_0040 at 43.621 kb on + strand, within Psest_0040 at 43.621 kb on + strand, within Psest_0040 at 43.621 kb on + strand, within Psest_0040 at 43.621 kb on + strand, within Psest_0040 at 43.621 kb on + strand, within Psest_0040 at 43.621 kb on + strand, within Psest_0040 at 43.621 kb on + strand, within Psest_0040 at 43.622 kb on + strand, within Psest_0040 at 43.622 kb on - strand, within Psest_0040 at 43.622 kb on - strand, within Psest_0040 at 43.622 kb on - strand, within Psest_0040 at 43.622 kb on - strand, within Psest_0040 at 43.622 kb on - strand, within Psest_0040 at 43.622 kb on - strand, within Psest_0040 at 43.622 kb on - strand, within Psest_0040 at 43.699 kb on + strand at 43.818 kb on + strand at 43.818 kb on + strand at 43.819 kb on - strand at 43.819 kb on - strand at 43.914 kb on + strand at 43.915 kb on - strand at 43.946 kb on + strand at 43.947 kb on - strand at 44.093 kb on + strand, within Psest_0041 at 44.339 kb on + strand, within Psest_0041 at 44.558 kb on + strand, within Psest_0041 at 44.558 kb on + strand, within Psest_0041 at 44.558 kb on + strand, within Psest_0041 at 44.559 kb on - strand, within Psest_0041 at 44.559 kb on - strand, within Psest_0041 at 44.559 kb on - strand, within Psest_0041 at 45.263 kb on + strand at 45.263 kb on + strand at 45.263 kb on + strand at 45.264 kb on - strand at 45.484 kb on - strand at 45.574 kb on + strand at 45.574 kb on + strand at 45.574 kb on + strand at 45.574 kb on + strand at 45.574 kb on - strand at 45.575 kb on - strand at 45.575 kb on - strand at 45.575 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Neomycin 0.0008 mg/ml remove 43,176 + +0.7 43,176 + -1.1 43,176 + -0.1 43,177 - -0.1 43,177 - +0.7 43,177 - -0.9 43,177 - +2.2 43,177 - -1.2 43,177 - -2.1 43,337 + +0.0 43,338 - +0.1 43,347 + -1.5 43,347 + +1.3 43,348 - -0.9 43,537 + Psest_0040 0.17 +0.1 43,538 - Psest_0040 0.17 -1.1 43,621 + Psest_0040 0.59 +1.0 43,621 + Psest_0040 0.59 -0.3 43,621 + Psest_0040 0.59 +1.3 43,621 + Psest_0040 0.59 +0.1 43,621 + Psest_0040 0.59 +3.6 43,621 + Psest_0040 0.59 +0.3 43,621 + Psest_0040 0.59 -0.6 43,621 + Psest_0040 0.59 +0.9 43,621 + Psest_0040 0.59 -0.3 43,622 + Psest_0040 0.60 -0.2 43,622 - Psest_0040 0.60 +1.4 43,622 - Psest_0040 0.60 +1.6 43,622 - Psest_0040 0.60 +0.4 43,622 - Psest_0040 0.60 -0.9 43,622 - Psest_0040 0.60 -0.5 43,622 - Psest_0040 0.60 -1.8 43,622 - Psest_0040 0.60 -0.3 43,699 + +1.5 43,818 + -0.3 43,818 + -0.5 43,819 - +0.1 43,819 - -2.2 43,914 + -1.8 43,915 - +0.1 43,946 + -2.1 43,947 - -0.6 44,093 + Psest_0041 0.14 -0.6 44,339 + Psest_0041 0.31 +2.7 44,558 + Psest_0041 0.45 +1.5 44,558 + Psest_0041 0.45 +1.8 44,558 + Psest_0041 0.45 +0.4 44,559 - Psest_0041 0.45 -1.3 44,559 - Psest_0041 0.45 -1.1 44,559 - Psest_0041 0.45 +0.7 45,263 + +0.3 45,263 + +3.5 45,263 + -0.0 45,264 - +1.0 45,484 - +0.1 45,574 + +1.4 45,574 + -0.3 45,574 + +1.0 45,574 + +1.7 45,574 - +0.1 45,575 - -0.6 45,575 - -0.3 45,575 - +1.5
Or see this region's nucleotide sequence