Experiment: Def Neomycin 0.0008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3313 and Psest_3314 are separated by 74 nucleotides Psest_3314 and Psest_3315 are separated by 7 nucleotides Psest_3315 and Psest_3316 are separated by 15 nucleotides Psest_3316 and Psest_3317 are separated by 14 nucleotides Psest_3317 and Psest_3318 are separated by 52 nucleotides
Psest_3313: Psest_3313 - RNA polymerase sigma-54 factor, at 3,476,690 to 3,478,198
_3313
Psest_3314: Psest_3314 - ribosomal subunit interface protein, at 3,478,273 to 3,478,581
_3314
Psest_3315: Psest_3315 - PTS IIA-like nitrogen-regulatory protein PtsN, at 3,478,589 to 3,479,053
_3315
Psest_3316: Psest_3316 - Predicted P-loop-containing kinase, at 3,479,069 to 3,479,926
_3316
Psest_3317: Psest_3317 - Phosphotransferase System HPr (HPr) Family, at 3,479,941 to 3,480,213
_3317
Psest_3318: Psest_3318 - Predicted Zn-dependent proteases and their inactivated homologs, at 3,480,266 to 3,481,612
_3318
Position (kb)
3479
3480 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3478.233 kb on + strand at 3478.266 kb on + strand at 3478.272 kb on + strand at 3478.307 kb on + strand, within Psest_3314 at 3478.307 kb on + strand, within Psest_3314 at 3478.307 kb on + strand, within Psest_3314 at 3478.327 kb on + strand, within Psest_3314 at 3478.483 kb on + strand, within Psest_3314 at 3478.483 kb on + strand, within Psest_3314 at 3478.483 kb on + strand, within Psest_3314 at 3478.483 kb on + strand, within Psest_3314 at 3478.483 kb on + strand, within Psest_3314 at 3478.483 kb on + strand, within Psest_3314 at 3478.483 kb on + strand, within Psest_3314 at 3478.483 kb on + strand, within Psest_3314 at 3478.483 kb on + strand, within Psest_3314 at 3478.483 kb on + strand, within Psest_3314 at 3478.483 kb on + strand, within Psest_3314 at 3478.483 kb on + strand, within Psest_3314 at 3478.483 kb on + strand, within Psest_3314 at 3478.483 kb on + strand, within Psest_3314 at 3478.483 kb on + strand, within Psest_3314 at 3478.483 kb on + strand, within Psest_3314 at 3478.483 kb on + strand, within Psest_3314 at 3478.483 kb on + strand, within Psest_3314 at 3478.483 kb on + strand, within Psest_3314 at 3478.483 kb on + strand, within Psest_3314 at 3478.483 kb on + strand, within Psest_3314 at 3478.550 kb on + strand, within Psest_3314 at 3478.550 kb on + strand, within Psest_3314 at 3479.085 kb on + strand at 3479.085 kb on + strand at 3479.085 kb on + strand at 3479.085 kb on + strand at 3479.085 kb on + strand at 3479.085 kb on + strand at 3479.086 kb on - strand at 3479.086 kb on - strand at 3479.086 kb on - strand at 3479.086 kb on - strand at 3479.086 kb on - strand at 3479.127 kb on + strand at 3479.128 kb on - strand at 3479.128 kb on - strand at 3479.147 kb on + strand at 3479.147 kb on + strand at 3479.210 kb on - strand, within Psest_3316 at 3479.217 kb on + strand, within Psest_3316 at 3479.218 kb on - strand, within Psest_3316 at 3479.314 kb on + strand, within Psest_3316 at 3479.334 kb on - strand, within Psest_3316 at 3479.499 kb on + strand, within Psest_3316 at 3479.499 kb on + strand, within Psest_3316 at 3479.499 kb on + strand, within Psest_3316 at 3479.500 kb on - strand, within Psest_3316 at 3479.500 kb on - strand, within Psest_3316 at 3479.500 kb on - strand, within Psest_3316 at 3479.500 kb on - strand, within Psest_3316 at 3479.500 kb on - strand, within Psest_3316 at 3479.500 kb on - strand, within Psest_3316 at 3479.623 kb on - strand, within Psest_3316 at 3479.706 kb on - strand, within Psest_3316 at 3479.706 kb on - strand, within Psest_3316 at 3479.757 kb on - strand, within Psest_3316 at 3479.801 kb on + strand, within Psest_3316 at 3479.801 kb on + strand, within Psest_3316 at 3479.801 kb on + strand, within Psest_3316 at 3479.801 kb on + strand, within Psest_3316 at 3479.801 kb on + strand, within Psest_3316 at 3479.801 kb on + strand, within Psest_3316 at 3479.801 kb on + strand, within Psest_3316 at 3479.802 kb on - strand, within Psest_3316 at 3479.802 kb on - strand, within Psest_3316 at 3479.802 kb on - strand, within Psest_3316 at 3479.849 kb on + strand at 3479.849 kb on + strand at 3479.849 kb on + strand at 3479.850 kb on - strand at 3479.924 kb on + strand at 3479.931 kb on - strand at 3479.931 kb on - strand at 3479.931 kb on - strand at 3479.931 kb on - strand at 3479.931 kb on - strand at 3479.931 kb on - strand at 3479.931 kb on - strand at 3479.936 kb on + strand at 3479.957 kb on + strand at 3479.957 kb on + strand at 3479.957 kb on + strand at 3479.957 kb on + strand at 3479.957 kb on + strand at 3479.957 kb on + strand at 3479.957 kb on + strand at 3479.957 kb on + strand at 3479.958 kb on - strand at 3480.117 kb on + strand, within Psest_3317 at 3480.117 kb on + strand, within Psest_3317 at 3480.117 kb on + strand, within Psest_3317 at 3480.117 kb on + strand, within Psest_3317 at 3480.117 kb on + strand, within Psest_3317 at 3480.117 kb on + strand, within Psest_3317 at 3480.117 kb on + strand, within Psest_3317 at 3480.118 kb on - strand, within Psest_3317 at 3480.194 kb on - strand at 3480.194 kb on - strand at 3480.392 kb on + strand at 3480.392 kb on + strand at 3480.470 kb on + strand, within Psest_3318 at 3480.470 kb on + strand, within Psest_3318 at 3480.471 kb on - strand, within Psest_3318 at 3480.471 kb on - strand, within Psest_3318 at 3480.471 kb on - strand, within Psest_3318 at 3480.625 kb on - strand, within Psest_3318 at 3480.639 kb on + strand, within Psest_3318 at 3480.639 kb on + strand, within Psest_3318 at 3480.639 kb on + strand, within Psest_3318 at 3480.639 kb on + strand, within Psest_3318 at 3480.639 kb on + strand, within Psest_3318 at 3480.639 kb on + strand, within Psest_3318 at 3480.639 kb on + strand, within Psest_3318 at 3480.640 kb on - strand, within Psest_3318 at 3480.640 kb on - strand, within Psest_3318 at 3480.640 kb on - strand, within Psest_3318 at 3480.640 kb on - strand, within Psest_3318 at 3480.640 kb on - strand, within Psest_3318 at 3480.640 kb on - strand, within Psest_3318 at 3480.640 kb on - strand, within Psest_3318 at 3480.640 kb on - strand, within Psest_3318 at 3480.640 kb on - strand, within Psest_3318 at 3480.640 kb on - strand, within Psest_3318 at 3480.640 kb on - strand, within Psest_3318 at 3480.679 kb on - strand, within Psest_3318 at 3480.702 kb on + strand, within Psest_3318 at 3480.703 kb on - strand, within Psest_3318 at 3480.703 kb on - strand, within Psest_3318 at 3480.795 kb on + strand, within Psest_3318 at 3480.796 kb on - strand, within Psest_3318 at 3480.796 kb on - strand, within Psest_3318 at 3480.796 kb on - strand, within Psest_3318 at 3480.796 kb on - strand, within Psest_3318 at 3480.796 kb on - strand, within Psest_3318
Per-strain Table
Position Strand Gene LocusTag Fraction Def Neomycin 0.0008 mg/ml remove 3,478,233 + -1.4 3,478,266 + +2.4 3,478,272 + +0.2 3,478,307 + Psest_3314 0.11 +0.3 3,478,307 + Psest_3314 0.11 +0.9 3,478,307 + Psest_3314 0.11 -1.7 3,478,327 + Psest_3314 0.17 -1.1 3,478,483 + Psest_3314 0.68 -0.5 3,478,483 + Psest_3314 0.68 +0.9 3,478,483 + Psest_3314 0.68 +0.1 3,478,483 + Psest_3314 0.68 -2.3 3,478,483 + Psest_3314 0.68 -2.0 3,478,483 + Psest_3314 0.68 +1.4 3,478,483 + Psest_3314 0.68 +0.4 3,478,483 + Psest_3314 0.68 +1.8 3,478,483 + Psest_3314 0.68 -0.1 3,478,483 + Psest_3314 0.68 -1.2 3,478,483 + Psest_3314 0.68 +1.9 3,478,483 + Psest_3314 0.68 +0.9 3,478,483 + Psest_3314 0.68 -0.3 3,478,483 + Psest_3314 0.68 +0.7 3,478,483 + Psest_3314 0.68 +1.8 3,478,483 + Psest_3314 0.68 +0.5 3,478,483 + Psest_3314 0.68 +1.9 3,478,483 + Psest_3314 0.68 -1.5 3,478,483 + Psest_3314 0.68 +0.3 3,478,483 + Psest_3314 0.68 -1.1 3,478,483 + Psest_3314 0.68 +1.8 3,478,550 + Psest_3314 0.90 +0.8 3,478,550 + Psest_3314 0.90 +0.8 3,479,085 + -2.0 3,479,085 + -2.7 3,479,085 + -0.6 3,479,085 + -1.2 3,479,085 + -2.1 3,479,085 + -1.7 3,479,086 - -3.7 3,479,086 - -2.4 3,479,086 - -3.1 3,479,086 - -3.2 3,479,086 - -3.3 3,479,127 + -0.5 3,479,128 - -3.2 3,479,128 - -2.7 3,479,147 + -0.3 3,479,147 + -2.8 3,479,210 - Psest_3316 0.16 -1.9 3,479,217 + Psest_3316 0.17 -3.3 3,479,218 - Psest_3316 0.17 -2.3 3,479,314 + Psest_3316 0.29 -1.8 3,479,334 - Psest_3316 0.31 -3.8 3,479,499 + Psest_3316 0.50 -1.4 3,479,499 + Psest_3316 0.50 -1.2 3,479,499 + Psest_3316 0.50 -1.5 3,479,500 - Psest_3316 0.50 -2.4 3,479,500 - Psest_3316 0.50 -1.2 3,479,500 - Psest_3316 0.50 -1.4 3,479,500 - Psest_3316 0.50 -3.3 3,479,500 - Psest_3316 0.50 -3.1 3,479,500 - Psest_3316 0.50 -2.3 3,479,623 - Psest_3316 0.65 -2.1 3,479,706 - Psest_3316 0.74 -2.0 3,479,706 - Psest_3316 0.74 -3.1 3,479,757 - Psest_3316 0.80 -2.1 3,479,801 + Psest_3316 0.85 -0.8 3,479,801 + Psest_3316 0.85 -2.3 3,479,801 + Psest_3316 0.85 -1.0 3,479,801 + Psest_3316 0.85 -1.6 3,479,801 + Psest_3316 0.85 -3.3 3,479,801 + Psest_3316 0.85 -0.4 3,479,801 + Psest_3316 0.85 -0.4 3,479,802 - Psest_3316 0.85 -1.7 3,479,802 - Psest_3316 0.85 -1.4 3,479,802 - Psest_3316 0.85 -1.3 3,479,849 + -0.9 3,479,849 + -0.3 3,479,849 + -2.0 3,479,850 - -2.8 3,479,924 + -1.4 3,479,931 - -1.5 3,479,931 - -2.3 3,479,931 - +0.9 3,479,931 - -0.6 3,479,931 - -1.8 3,479,931 - -0.8 3,479,931 - -1.3 3,479,936 + -0.1 3,479,957 + -1.3 3,479,957 + -1.6 3,479,957 + -1.1 3,479,957 + -3.9 3,479,957 + -2.3 3,479,957 + -1.0 3,479,957 + -1.0 3,479,957 + -0.9 3,479,958 - -1.7 3,480,117 + Psest_3317 0.64 -1.8 3,480,117 + Psest_3317 0.64 +0.5 3,480,117 + Psest_3317 0.64 -1.4 3,480,117 + Psest_3317 0.64 -1.8 3,480,117 + Psest_3317 0.64 -1.2 3,480,117 + Psest_3317 0.64 -1.2 3,480,117 + Psest_3317 0.64 -4.3 3,480,118 - Psest_3317 0.65 +0.6 3,480,194 - -1.5 3,480,194 - +0.5 3,480,392 + -0.3 3,480,392 + -0.2 3,480,470 + Psest_3318 0.15 -0.1 3,480,470 + Psest_3318 0.15 -2.7 3,480,471 - Psest_3318 0.15 +0.7 3,480,471 - Psest_3318 0.15 -1.0 3,480,471 - Psest_3318 0.15 +0.5 3,480,625 - Psest_3318 0.27 -2.3 3,480,639 + Psest_3318 0.28 +0.1 3,480,639 + Psest_3318 0.28 +1.2 3,480,639 + Psest_3318 0.28 +0.2 3,480,639 + Psest_3318 0.28 +0.8 3,480,639 + Psest_3318 0.28 -0.4 3,480,639 + Psest_3318 0.28 -1.2 3,480,639 + Psest_3318 0.28 -0.4 3,480,640 - Psest_3318 0.28 +0.5 3,480,640 - Psest_3318 0.28 +0.7 3,480,640 - Psest_3318 0.28 +0.5 3,480,640 - Psest_3318 0.28 -1.4 3,480,640 - Psest_3318 0.28 -0.5 3,480,640 - Psest_3318 0.28 +0.8 3,480,640 - Psest_3318 0.28 +1.2 3,480,640 - Psest_3318 0.28 +0.5 3,480,640 - Psest_3318 0.28 -0.7 3,480,640 - Psest_3318 0.28 +1.1 3,480,640 - Psest_3318 0.28 -2.5 3,480,679 - Psest_3318 0.31 +1.1 3,480,702 + Psest_3318 0.32 -1.0 3,480,703 - Psest_3318 0.32 +0.1 3,480,703 - Psest_3318 0.32 -1.4 3,480,795 + Psest_3318 0.39 -1.1 3,480,796 - Psest_3318 0.39 +1.3 3,480,796 - Psest_3318 0.39 +0.1 3,480,796 - Psest_3318 0.39 +0.4 3,480,796 - Psest_3318 0.39 +0.0 3,480,796 - Psest_3318 0.39 -0.0
Or see this region's nucleotide sequence