Experiment: Def Neomycin 0.0008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2721 and Psest_2722 are separated by 205 nucleotides Psest_2722 and Psest_2723 are separated by 153 nucleotides Psest_2723 and Psest_2724 are separated by 34 nucleotides Psest_2724 and Psest_2725 are separated by 227 nucleotides Psest_2725 and Psest_2726 are separated by 35 nucleotides Psest_2726 and Psest_2727 are separated by 130 nucleotides
Psest_2721: Psest_2721 - HD-GYP domain, at 2,909,909 to 2,911,198
_2721
Psest_2722: Psest_2722 - tRNA_Ser_GGA, at 2,911,404 to 2,911,493
_2722
Psest_2723: Psest_2723 - Uncharacterized conserved protein, at 2,911,647 to 2,912,072
_2723
Psest_2724: Psest_2724 - hypothetical protein, at 2,912,107 to 2,912,619
_2724
Psest_2725: Psest_2725 - hypothetical protein, at 2,912,847 to 2,912,942
_2725
Psest_2726: Psest_2726 - Rhodanese-related sulfurtransferase, at 2,912,978 to 2,913,334
_2726
Psest_2727: Psest_2727 - Small-conductance mechanosensitive channel, at 2,913,465 to 2,914,607
_2727
Position (kb)
2912
2913 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2911.109 kb on - strand at 2911.312 kb on + strand at 2911.494 kb on + strand at 2911.548 kb on + strand at 2911.571 kb on + strand at 2911.571 kb on + strand at 2911.571 kb on + strand at 2911.571 kb on + strand at 2911.571 kb on + strand at 2911.571 kb on + strand at 2911.607 kb on + strand at 2911.659 kb on + strand at 2911.660 kb on - strand at 2911.660 kb on - strand at 2911.698 kb on + strand, within Psest_2723 at 2911.840 kb on - strand, within Psest_2723 at 2911.855 kb on + strand, within Psest_2723 at 2911.856 kb on - strand, within Psest_2723 at 2911.969 kb on + strand, within Psest_2723 at 2911.969 kb on + strand, within Psest_2723 at 2911.969 kb on + strand, within Psest_2723 at 2911.970 kb on - strand, within Psest_2723 at 2911.970 kb on - strand, within Psest_2723 at 2911.970 kb on - strand, within Psest_2723 at 2912.108 kb on + strand at 2912.272 kb on - strand, within Psest_2724 at 2912.272 kb on - strand, within Psest_2724 at 2912.473 kb on - strand, within Psest_2724 at 2912.544 kb on + strand, within Psest_2724 at 2912.594 kb on - strand at 2912.641 kb on - strand at 2912.692 kb on + strand at 2912.719 kb on + strand at 2912.719 kb on + strand at 2912.720 kb on - strand at 2912.722 kb on - strand at 2912.741 kb on - strand at 2912.766 kb on - strand at 2912.786 kb on - strand at 2912.833 kb on + strand at 2912.833 kb on + strand at 2912.833 kb on + strand at 2912.834 kb on - strand at 2912.834 kb on - strand at 2912.930 kb on + strand, within Psest_2725 at 2912.930 kb on + strand, within Psest_2725 at 2912.931 kb on - strand, within Psest_2725 at 2912.931 kb on - strand, within Psest_2725 at 2912.933 kb on - strand at 2912.933 kb on - strand at 2913.004 kb on - strand at 2913.129 kb on + strand, within Psest_2726 at 2913.129 kb on + strand, within Psest_2726 at 2913.215 kb on + strand, within Psest_2726 at 2913.395 kb on + strand at 2913.395 kb on + strand at 2913.395 kb on + strand at 2913.395 kb on + strand at 2913.395 kb on + strand at 2913.395 kb on + strand at 2913.395 kb on + strand at 2913.396 kb on - strand at 2913.396 kb on - strand at 2913.396 kb on - strand at 2913.396 kb on - strand at 2913.396 kb on - strand at 2913.396 kb on - strand at 2913.396 kb on - strand at 2913.461 kb on - strand at 2913.461 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Neomycin 0.0008 mg/ml remove 2,911,109 - -1.8 2,911,312 + -0.3 2,911,494 + +0.2 2,911,548 + +1.7 2,911,571 + -2.5 2,911,571 + -0.6 2,911,571 + +0.9 2,911,571 + -0.7 2,911,571 + -1.6 2,911,571 + +0.6 2,911,607 + +1.4 2,911,659 + -1.4 2,911,660 - -1.0 2,911,660 - +0.1 2,911,698 + Psest_2723 0.12 -1.4 2,911,840 - Psest_2723 0.45 -0.4 2,911,855 + Psest_2723 0.49 -1.1 2,911,856 - Psest_2723 0.49 +0.1 2,911,969 + Psest_2723 0.76 +1.3 2,911,969 + Psest_2723 0.76 -0.0 2,911,969 + Psest_2723 0.76 -1.1 2,911,970 - Psest_2723 0.76 +1.3 2,911,970 - Psest_2723 0.76 -1.0 2,911,970 - Psest_2723 0.76 +0.4 2,912,108 + -1.8 2,912,272 - Psest_2724 0.32 -2.1 2,912,272 - Psest_2724 0.32 -1.6 2,912,473 - Psest_2724 0.71 -1.6 2,912,544 + Psest_2724 0.85 -0.8 2,912,594 - +2.6 2,912,641 - +0.9 2,912,692 + +2.3 2,912,719 + -0.5 2,912,719 + +0.4 2,912,720 - +1.2 2,912,722 - -0.4 2,912,741 - -1.4 2,912,766 - -0.6 2,912,786 - +0.7 2,912,833 + -0.4 2,912,833 + +1.4 2,912,833 + -0.9 2,912,834 - -0.6 2,912,834 - -0.1 2,912,930 + Psest_2725 0.86 +1.8 2,912,930 + Psest_2725 0.86 -0.5 2,912,931 - Psest_2725 0.88 -0.0 2,912,931 - Psest_2725 0.88 +2.0 2,912,933 - +0.7 2,912,933 - -0.2 2,913,004 - -1.4 2,913,129 + Psest_2726 0.42 -1.3 2,913,129 + Psest_2726 0.42 -0.0 2,913,215 + Psest_2726 0.66 -0.2 2,913,395 + +0.2 2,913,395 + +0.9 2,913,395 + -0.5 2,913,395 + -1.6 2,913,395 + -0.4 2,913,395 + -0.6 2,913,395 + +0.1 2,913,396 - -0.3 2,913,396 - -1.4 2,913,396 - +1.6 2,913,396 - +0.5 2,913,396 - -0.1 2,913,396 - -0.7 2,913,396 - -0.3 2,913,461 - -2.3 2,913,461 - -0.1
Or see this region's nucleotide sequence