Experiment: Def Neomycin 0.0008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2598 and Psest_2599 are separated by 857 nucleotides Psest_2599 and Psest_2600 are separated by 73 nucleotides
Psest_2598: Psest_2598 - tRNA_Met_CAT, at 2,779,221 to 2,779,297
_2598
Psest_2599: Psest_2599 - hypothetical protein, at 2,780,155 to 2,780,928
_2599
Psest_2600: Psest_2600 - Predicted pyridoxal phosphate-dependent enzyme apparently involved in regulation of cell wall biogenesis, at 2,781,002 to 2,782,180
_2600
Position (kb)
2780
2781 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2779.386 kb on + strand at 2779.387 kb on - strand at 2779.425 kb on - strand at 2779.448 kb on + strand at 2779.449 kb on - strand at 2779.449 kb on - strand at 2779.476 kb on + strand at 2779.518 kb on - strand at 2779.522 kb on - strand at 2779.533 kb on + strand at 2779.597 kb on - strand at 2779.687 kb on - strand at 2779.705 kb on + strand at 2779.706 kb on - strand at 2779.781 kb on - strand at 2779.822 kb on - strand at 2779.846 kb on - strand at 2779.846 kb on - strand at 2779.912 kb on + strand at 2779.927 kb on - strand at 2779.931 kb on + strand at 2779.931 kb on + strand at 2779.932 kb on - strand at 2779.932 kb on - strand at 2779.998 kb on + strand at 2780.007 kb on - strand at 2780.010 kb on - strand at 2780.062 kb on - strand at 2780.151 kb on + strand at 2780.152 kb on - strand at 2780.215 kb on + strand at 2780.216 kb on - strand at 2780.249 kb on + strand, within Psest_2599 at 2780.317 kb on + strand, within Psest_2599 at 2780.317 kb on + strand, within Psest_2599 at 2780.317 kb on + strand, within Psest_2599 at 2780.411 kb on - strand, within Psest_2599 at 2780.452 kb on + strand, within Psest_2599 at 2780.452 kb on + strand, within Psest_2599 at 2780.452 kb on + strand, within Psest_2599 at 2780.452 kb on + strand, within Psest_2599 at 2780.459 kb on + strand, within Psest_2599 at 2780.460 kb on - strand, within Psest_2599 at 2780.513 kb on - strand, within Psest_2599 at 2780.618 kb on - strand, within Psest_2599 at 2780.618 kb on - strand, within Psest_2599 at 2780.618 kb on - strand, within Psest_2599 at 2780.618 kb on - strand, within Psest_2599 at 2780.618 kb on - strand, within Psest_2599 at 2780.618 kb on - strand, within Psest_2599 at 2781.053 kb on + strand at 2781.053 kb on + strand at 2781.053 kb on + strand at 2781.054 kb on - strand at 2781.054 kb on - strand at 2781.054 kb on - strand at 2781.054 kb on - strand at 2781.068 kb on + strand at 2781.069 kb on - strand at 2781.134 kb on + strand, within Psest_2600 at 2781.135 kb on - strand, within Psest_2600 at 2781.135 kb on - strand, within Psest_2600 at 2781.347 kb on - strand, within Psest_2600 at 2781.452 kb on + strand, within Psest_2600 at 2781.452 kb on + strand, within Psest_2600 at 2781.452 kb on + strand, within Psest_2600 at 2781.453 kb on - strand, within Psest_2600 at 2781.453 kb on - strand, within Psest_2600 at 2781.453 kb on - strand, within Psest_2600 at 2781.453 kb on - strand, within Psest_2600 at 2781.708 kb on + strand, within Psest_2600 at 2781.708 kb on + strand, within Psest_2600 at 2781.708 kb on + strand, within Psest_2600 at 2781.708 kb on + strand, within Psest_2600 at 2781.708 kb on + strand, within Psest_2600 at 2781.708 kb on + strand, within Psest_2600 at 2781.708 kb on + strand, within Psest_2600 at 2781.708 kb on + strand, within Psest_2600 at 2781.709 kb on - strand, within Psest_2600 at 2781.709 kb on - strand, within Psest_2600 at 2781.709 kb on - strand, within Psest_2600 at 2781.709 kb on - strand, within Psest_2600 at 2781.709 kb on - strand, within Psest_2600 at 2781.830 kb on + strand, within Psest_2600 at 2781.830 kb on + strand, within Psest_2600 at 2781.830 kb on + strand, within Psest_2600 at 2781.830 kb on + strand, within Psest_2600 at 2781.831 kb on - strand, within Psest_2600 at 2781.831 kb on - strand, within Psest_2600 at 2781.831 kb on - strand, within Psest_2600 at 2781.831 kb on - strand, within Psest_2600 at 2781.831 kb on - strand, within Psest_2600 at 2781.831 kb on - strand, within Psest_2600
Per-strain Table
Position Strand Gene LocusTag Fraction Def Neomycin 0.0008 mg/ml remove 2,779,386 + -2.8 2,779,387 - -1.2 2,779,425 - +0.1 2,779,448 + +1.2 2,779,449 - +0.9 2,779,449 - +0.3 2,779,476 + -0.8 2,779,518 - +0.3 2,779,522 - +1.1 2,779,533 + +0.8 2,779,597 - +0.5 2,779,687 - +0.5 2,779,705 + -1.2 2,779,706 - +0.3 2,779,781 - -0.2 2,779,822 - -0.8 2,779,846 - +0.7 2,779,846 - +0.1 2,779,912 + -1.9 2,779,927 - -0.8 2,779,931 + -0.1 2,779,931 + -2.5 2,779,932 - +0.6 2,779,932 - +0.2 2,779,998 + -1.9 2,780,007 - -0.0 2,780,010 - +0.3 2,780,062 - +0.6 2,780,151 + -2.0 2,780,152 - +0.3 2,780,215 + +2.6 2,780,216 - +0.8 2,780,249 + Psest_2599 0.12 +0.5 2,780,317 + Psest_2599 0.21 -2.2 2,780,317 + Psest_2599 0.21 -1.1 2,780,317 + Psest_2599 0.21 -2.8 2,780,411 - Psest_2599 0.33 -0.3 2,780,452 + Psest_2599 0.38 -0.9 2,780,452 + Psest_2599 0.38 -1.9 2,780,452 + Psest_2599 0.38 +0.1 2,780,452 + Psest_2599 0.38 -1.1 2,780,459 + Psest_2599 0.39 -2.4 2,780,460 - Psest_2599 0.39 +0.1 2,780,513 - Psest_2599 0.46 -0.6 2,780,618 - Psest_2599 0.60 -0.1 2,780,618 - Psest_2599 0.60 +1.0 2,780,618 - Psest_2599 0.60 -0.8 2,780,618 - Psest_2599 0.60 -0.8 2,780,618 - Psest_2599 0.60 +0.5 2,780,618 - Psest_2599 0.60 -0.0 2,781,053 + -0.2 2,781,053 + -0.0 2,781,053 + -0.7 2,781,054 - -0.3 2,781,054 - -0.7 2,781,054 - -0.4 2,781,054 - -0.8 2,781,068 + +0.2 2,781,069 - -0.1 2,781,134 + Psest_2600 0.11 +0.9 2,781,135 - Psest_2600 0.11 -1.3 2,781,135 - Psest_2600 0.11 +0.1 2,781,347 - Psest_2600 0.29 -2.3 2,781,452 + Psest_2600 0.38 -0.1 2,781,452 + Psest_2600 0.38 +1.2 2,781,452 + Psest_2600 0.38 -0.8 2,781,453 - Psest_2600 0.38 -0.7 2,781,453 - Psest_2600 0.38 -0.5 2,781,453 - Psest_2600 0.38 -2.0 2,781,453 - Psest_2600 0.38 -0.0 2,781,708 + Psest_2600 0.60 -0.4 2,781,708 + Psest_2600 0.60 +0.2 2,781,708 + Psest_2600 0.60 -0.2 2,781,708 + Psest_2600 0.60 +1.1 2,781,708 + Psest_2600 0.60 +0.4 2,781,708 + Psest_2600 0.60 -1.6 2,781,708 + Psest_2600 0.60 +0.8 2,781,708 + Psest_2600 0.60 -1.8 2,781,709 - Psest_2600 0.60 +0.1 2,781,709 - Psest_2600 0.60 +0.8 2,781,709 - Psest_2600 0.60 -2.8 2,781,709 - Psest_2600 0.60 +1.4 2,781,709 - Psest_2600 0.60 +1.3 2,781,830 + Psest_2600 0.70 +0.9 2,781,830 + Psest_2600 0.70 -1.4 2,781,830 + Psest_2600 0.70 -0.2 2,781,830 + Psest_2600 0.70 -0.2 2,781,831 - Psest_2600 0.70 +0.7 2,781,831 - Psest_2600 0.70 +0.3 2,781,831 - Psest_2600 0.70 +0.3 2,781,831 - Psest_2600 0.70 -0.6 2,781,831 - Psest_2600 0.70 +1.2 2,781,831 - Psest_2600 0.70 +0.4
Or see this region's nucleotide sequence