Experiment: Def Neomycin 0.0008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2390 and Psest_2391 are separated by 85 nucleotides Psest_2391 and Psest_2392 are separated by 173 nucleotides Psest_2392 and Psest_2393 are separated by 36 nucleotides
Psest_2390: Psest_2390 - hypothetical protein, at 2,541,821 to 2,542,348
_2390
Psest_2391: Psest_2391 - PAS domain S-box, at 2,542,434 to 2,542,880
_2391
Psest_2392: Psest_2392 - PAS domain S-box/diguanylate cyclase (GGDEF) domain, at 2,543,054 to 2,545,714
_2392
Psest_2393: Psest_2393 - ferrochelatase, at 2,545,751 to 2,546,776
_2393
Position (kb)
2543
2544
2545
2546 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2542.220 kb on + strand, within Psest_2390 at 2542.221 kb on - strand, within Psest_2390 at 2542.247 kb on + strand, within Psest_2390 at 2542.247 kb on + strand, within Psest_2390 at 2542.247 kb on + strand, within Psest_2390 at 2542.248 kb on - strand, within Psest_2390 at 2542.248 kb on - strand, within Psest_2390 at 2542.248 kb on - strand, within Psest_2390 at 2542.248 kb on - strand, within Psest_2390 at 2542.248 kb on - strand, within Psest_2390 at 2542.248 kb on - strand, within Psest_2390 at 2542.398 kb on + strand at 2542.527 kb on + strand, within Psest_2391 at 2542.528 kb on - strand, within Psest_2391 at 2542.689 kb on + strand, within Psest_2391 at 2542.689 kb on + strand, within Psest_2391 at 2542.689 kb on + strand, within Psest_2391 at 2542.690 kb on - strand, within Psest_2391 at 2542.764 kb on + strand, within Psest_2391 at 2542.764 kb on + strand, within Psest_2391 at 2542.764 kb on + strand, within Psest_2391 at 2542.765 kb on - strand, within Psest_2391 at 2542.765 kb on - strand, within Psest_2391 at 2542.902 kb on + strand at 2542.903 kb on - strand at 2542.954 kb on + strand at 2542.954 kb on + strand at 2542.954 kb on + strand at 2542.955 kb on - strand at 2542.960 kb on - strand at 2542.960 kb on - strand at 2543.263 kb on + strand at 2543.293 kb on - strand at 2543.355 kb on + strand, within Psest_2392 at 2543.370 kb on + strand, within Psest_2392 at 2543.405 kb on + strand, within Psest_2392 at 2543.405 kb on + strand, within Psest_2392 at 2543.601 kb on + strand, within Psest_2392 at 2543.644 kb on - strand, within Psest_2392 at 2543.723 kb on + strand, within Psest_2392 at 2543.723 kb on + strand, within Psest_2392 at 2543.724 kb on - strand, within Psest_2392 at 2543.724 kb on - strand, within Psest_2392 at 2543.724 kb on - strand, within Psest_2392 at 2543.883 kb on - strand, within Psest_2392 at 2544.065 kb on - strand, within Psest_2392 at 2544.300 kb on + strand, within Psest_2392 at 2544.300 kb on + strand, within Psest_2392 at 2544.300 kb on + strand, within Psest_2392 at 2544.300 kb on + strand, within Psest_2392 at 2544.301 kb on - strand, within Psest_2392 at 2544.305 kb on - strand, within Psest_2392 at 2544.329 kb on + strand, within Psest_2392 at 2544.329 kb on + strand, within Psest_2392 at 2544.398 kb on + strand, within Psest_2392 at 2544.399 kb on - strand, within Psest_2392 at 2544.600 kb on + strand, within Psest_2392 at 2544.740 kb on + strand, within Psest_2392 at 2544.811 kb on + strand, within Psest_2392 at 2544.811 kb on + strand, within Psest_2392 at 2544.851 kb on + strand, within Psest_2392 at 2544.852 kb on - strand, within Psest_2392 at 2544.852 kb on - strand, within Psest_2392 at 2544.852 kb on - strand, within Psest_2392 at 2544.852 kb on - strand, within Psest_2392 at 2544.852 kb on - strand, within Psest_2392 at 2545.132 kb on - strand, within Psest_2392 at 2545.132 kb on - strand, within Psest_2392 at 2545.132 kb on - strand, within Psest_2392 at 2545.243 kb on - strand, within Psest_2392 at 2545.330 kb on + strand, within Psest_2392 at 2545.330 kb on + strand, within Psest_2392 at 2545.330 kb on + strand, within Psest_2392 at 2545.331 kb on - strand, within Psest_2392 at 2545.425 kb on - strand, within Psest_2392 at 2545.629 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Neomycin 0.0008 mg/ml remove 2,542,220 + Psest_2390 0.76 +0.7 2,542,221 - Psest_2390 0.76 +1.3 2,542,247 + Psest_2390 0.81 +0.1 2,542,247 + Psest_2390 0.81 +1.7 2,542,247 + Psest_2390 0.81 -0.1 2,542,248 - Psest_2390 0.81 -1.3 2,542,248 - Psest_2390 0.81 +2.0 2,542,248 - Psest_2390 0.81 +1.1 2,542,248 - Psest_2390 0.81 -0.1 2,542,248 - Psest_2390 0.81 +0.3 2,542,248 - Psest_2390 0.81 +0.0 2,542,398 + -0.0 2,542,527 + Psest_2391 0.21 +2.5 2,542,528 - Psest_2391 0.21 -0.0 2,542,689 + Psest_2391 0.57 +0.3 2,542,689 + Psest_2391 0.57 +2.3 2,542,689 + Psest_2391 0.57 +0.7 2,542,690 - Psest_2391 0.57 +0.5 2,542,764 + Psest_2391 0.74 +3.2 2,542,764 + Psest_2391 0.74 +0.4 2,542,764 + Psest_2391 0.74 -1.9 2,542,765 - Psest_2391 0.74 -0.5 2,542,765 - Psest_2391 0.74 +1.4 2,542,902 + -0.8 2,542,903 - +1.8 2,542,954 + -1.6 2,542,954 + -0.7 2,542,954 + +0.1 2,542,955 - -1.6 2,542,960 - -1.8 2,542,960 - +0.5 2,543,263 + +1.3 2,543,293 - -0.3 2,543,355 + Psest_2392 0.11 +2.0 2,543,370 + Psest_2392 0.12 -1.1 2,543,405 + Psest_2392 0.13 -1.5 2,543,405 + Psest_2392 0.13 +0.7 2,543,601 + Psest_2392 0.21 +0.1 2,543,644 - Psest_2392 0.22 +1.0 2,543,723 + Psest_2392 0.25 +0.8 2,543,723 + Psest_2392 0.25 +0.4 2,543,724 - Psest_2392 0.25 +2.0 2,543,724 - Psest_2392 0.25 -1.8 2,543,724 - Psest_2392 0.25 +0.9 2,543,883 - Psest_2392 0.31 -0.3 2,544,065 - Psest_2392 0.38 -0.6 2,544,300 + Psest_2392 0.47 +0.2 2,544,300 + Psest_2392 0.47 -0.8 2,544,300 + Psest_2392 0.47 -0.0 2,544,300 + Psest_2392 0.47 +1.5 2,544,301 - Psest_2392 0.47 -0.1 2,544,305 - Psest_2392 0.47 +0.7 2,544,329 + Psest_2392 0.48 +3.4 2,544,329 + Psest_2392 0.48 +0.8 2,544,398 + Psest_2392 0.51 -1.4 2,544,399 - Psest_2392 0.51 -0.0 2,544,600 + Psest_2392 0.58 +1.0 2,544,740 + Psest_2392 0.63 -0.4 2,544,811 + Psest_2392 0.66 +0.7 2,544,811 + Psest_2392 0.66 +1.7 2,544,851 + Psest_2392 0.68 -0.9 2,544,852 - Psest_2392 0.68 +0.1 2,544,852 - Psest_2392 0.68 +1.0 2,544,852 - Psest_2392 0.68 +1.6 2,544,852 - Psest_2392 0.68 +0.8 2,544,852 - Psest_2392 0.68 -1.5 2,545,132 - Psest_2392 0.78 -0.7 2,545,132 - Psest_2392 0.78 -1.1 2,545,132 - Psest_2392 0.78 +0.7 2,545,243 - Psest_2392 0.82 +0.6 2,545,330 + Psest_2392 0.86 +0.7 2,545,330 + Psest_2392 0.86 +2.1 2,545,330 + Psest_2392 0.86 +0.6 2,545,331 - Psest_2392 0.86 +1.0 2,545,425 - Psest_2392 0.89 -0.6 2,545,629 - -1.6
Or see this region's nucleotide sequence