Experiment: Def Neomycin 0.0008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2252 and Psest_2253 are separated by 8 nucleotides Psest_2253 and Psest_2254 are separated by 112 nucleotides Psest_2254 and Psest_2255 are separated by 0 nucleotides Psest_2255 and Psest_2256 are separated by 152 nucleotides Psest_2256 and Psest_2257 are separated by 46 nucleotides Psest_2257 and Psest_2258 are separated by 69 nucleotides
Psest_2252: Psest_2252 - Predicted permeases, at 2,401,533 to 2,402,909
_2252
Psest_2253: Psest_2253 - small redox-active disulfide protein 2, at 2,402,918 to 2,403,148
_2253
Psest_2254: Psest_2254 - hypothetical protein, at 2,403,261 to 2,403,455
_2254
Psest_2255: Psest_2255 - hypothetical protein, at 2,403,456 to 2,403,701
_2255
Psest_2256: Psest_2256 - tRNA_Cys_GCA, at 2,403,854 to 2,403,927
_2256
Psest_2257: Psest_2257 - tRNA_Gly_GCC, at 2,403,974 to 2,404,049
_2257
Psest_2258: Psest_2258 - CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase, at 2,404,119 to 2,404,733
_2258
Position (kb)
2403
2404 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2402.335 kb on + strand, within Psest_2252 at 2402.443 kb on + strand, within Psest_2252 at 2402.443 kb on + strand, within Psest_2252 at 2402.443 kb on + strand, within Psest_2252 at 2402.444 kb on - strand, within Psest_2252 at 2402.444 kb on - strand, within Psest_2252 at 2402.464 kb on + strand, within Psest_2252 at 2402.465 kb on - strand, within Psest_2252 at 2402.689 kb on - strand, within Psest_2252 at 2402.689 kb on - strand, within Psest_2252 at 2402.689 kb on - strand, within Psest_2252 at 2402.847 kb on + strand at 2402.847 kb on + strand at 2402.887 kb on - strand at 2402.930 kb on - strand at 2402.930 kb on - strand at 2402.930 kb on - strand at 2402.933 kb on + strand at 2402.933 kb on + strand at 2402.933 kb on + strand at 2402.933 kb on + strand at 2402.933 kb on + strand at 2402.933 kb on + strand at 2402.933 kb on + strand at 2402.933 kb on + strand at 2402.933 kb on + strand at 2402.934 kb on - strand at 2402.934 kb on - strand at 2402.934 kb on - strand at 2403.033 kb on + strand, within Psest_2253 at 2403.033 kb on + strand, within Psest_2253 at 2403.033 kb on + strand, within Psest_2253 at 2403.033 kb on + strand, within Psest_2253 at 2403.034 kb on - strand, within Psest_2253 at 2403.082 kb on - strand, within Psest_2253 at 2403.218 kb on - strand at 2403.218 kb on - strand at 2403.218 kb on - strand at 2403.218 kb on - strand at 2403.232 kb on + strand at 2403.232 kb on + strand at 2403.232 kb on + strand at 2403.232 kb on + strand at 2403.232 kb on + strand at 2403.232 kb on + strand at 2403.232 kb on + strand at 2403.232 kb on + strand at 2403.232 kb on + strand at 2403.232 kb on + strand at 2403.232 kb on + strand at 2403.233 kb on - strand at 2403.233 kb on - strand at 2403.233 kb on - strand at 2403.234 kb on + strand at 2403.234 kb on + strand at 2403.234 kb on + strand at 2403.234 kb on + strand at 2403.234 kb on + strand at 2403.234 kb on + strand at 2403.234 kb on + strand at 2403.234 kb on + strand at 2403.234 kb on + strand at 2403.234 kb on + strand at 2403.234 kb on + strand at 2403.234 kb on + strand at 2403.235 kb on - strand at 2403.235 kb on - strand at 2403.235 kb on - strand at 2403.235 kb on - strand at 2403.235 kb on - strand at 2403.244 kb on + strand at 2403.279 kb on + strand at 2403.279 kb on + strand at 2403.279 kb on + strand at 2403.279 kb on + strand at 2403.279 kb on + strand at 2403.279 kb on + strand at 2403.279 kb on + strand at 2403.279 kb on + strand at 2403.280 kb on - strand at 2403.280 kb on - strand at 2403.280 kb on - strand at 2403.478 kb on + strand at 2403.478 kb on + strand at 2403.479 kb on - strand at 2403.479 kb on - strand at 2403.479 kb on - strand at 2403.735 kb on + strand at 2403.735 kb on + strand at 2403.735 kb on + strand at 2403.735 kb on + strand at 2403.736 kb on - strand at 2403.736 kb on - strand at 2403.736 kb on - strand at 2403.736 kb on - strand at 2403.736 kb on - strand at 2403.736 kb on - strand at 2403.737 kb on + strand at 2403.737 kb on + strand at 2403.737 kb on + strand at 2403.737 kb on + strand at 2403.737 kb on + strand at 2403.737 kb on + strand at 2403.737 kb on + strand at 2403.737 kb on + strand at 2403.737 kb on + strand at 2403.737 kb on + strand at 2403.738 kb on - strand at 2403.738 kb on - strand at 2403.738 kb on - strand at 2403.738 kb on - strand at 2403.738 kb on - strand at 2403.738 kb on - strand at 2403.738 kb on - strand at 2403.738 kb on - strand at 2403.738 kb on - strand at 2403.738 kb on - strand at 2403.738 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Neomycin 0.0008 mg/ml remove 2,402,335 + Psest_2252 0.58 -1.1 2,402,443 + Psest_2252 0.66 +0.8 2,402,443 + Psest_2252 0.66 +0.1 2,402,443 + Psest_2252 0.66 +0.2 2,402,444 - Psest_2252 0.66 +1.0 2,402,444 - Psest_2252 0.66 -0.4 2,402,464 + Psest_2252 0.68 -2.0 2,402,465 - Psest_2252 0.68 -1.7 2,402,689 - Psest_2252 0.84 -1.8 2,402,689 - Psest_2252 0.84 +2.0 2,402,689 - Psest_2252 0.84 +2.0 2,402,847 + +0.4 2,402,847 + +0.7 2,402,887 - -0.7 2,402,930 - +1.2 2,402,930 - +1.1 2,402,930 - +0.7 2,402,933 + +1.1 2,402,933 + +1.5 2,402,933 + +1.6 2,402,933 + -1.0 2,402,933 + +1.1 2,402,933 + -2.0 2,402,933 + -2.1 2,402,933 + +0.1 2,402,933 + -1.1 2,402,934 - -0.6 2,402,934 - -2.5 2,402,934 - -2.7 2,403,033 + Psest_2253 0.50 -0.2 2,403,033 + Psest_2253 0.50 -1.9 2,403,033 + Psest_2253 0.50 -0.8 2,403,033 + Psest_2253 0.50 -3.1 2,403,034 - Psest_2253 0.50 +1.4 2,403,082 - Psest_2253 0.71 -0.4 2,403,218 - -1.4 2,403,218 - -1.2 2,403,218 - -0.5 2,403,218 - -0.1 2,403,232 + +0.0 2,403,232 + -0.6 2,403,232 + -2.0 2,403,232 + -0.4 2,403,232 + -0.5 2,403,232 + -1.0 2,403,232 + -0.4 2,403,232 + -0.3 2,403,232 + -1.8 2,403,232 + +0.6 2,403,232 + +0.9 2,403,233 - -0.1 2,403,233 - +0.6 2,403,233 - -0.1 2,403,234 + +0.2 2,403,234 + -2.1 2,403,234 + +0.2 2,403,234 + +0.5 2,403,234 + -1.0 2,403,234 + -0.5 2,403,234 + -0.3 2,403,234 + -1.1 2,403,234 + -0.4 2,403,234 + -0.3 2,403,234 + -1.0 2,403,234 + +0.1 2,403,235 - +0.8 2,403,235 - +0.8 2,403,235 - -0.1 2,403,235 - +1.8 2,403,235 - -0.1 2,403,244 + -0.6 2,403,279 + -0.8 2,403,279 + -1.8 2,403,279 + -2.5 2,403,279 + -0.8 2,403,279 + +0.8 2,403,279 + +0.8 2,403,279 + +0.6 2,403,279 + -2.9 2,403,280 - +1.5 2,403,280 - +0.4 2,403,280 - +0.7 2,403,478 + +0.2 2,403,478 + +1.3 2,403,479 - +0.6 2,403,479 - +0.1 2,403,479 - +1.7 2,403,735 + -0.5 2,403,735 + -1.7 2,403,735 + +0.3 2,403,735 + +1.1 2,403,736 - +0.7 2,403,736 - -0.8 2,403,736 - -0.9 2,403,736 - -0.8 2,403,736 - +1.7 2,403,736 - -1.4 2,403,737 + +1.9 2,403,737 + -0.0 2,403,737 + +1.9 2,403,737 + -0.9 2,403,737 + +2.9 2,403,737 + -0.5 2,403,737 + +2.3 2,403,737 + +0.6 2,403,737 + -1.8 2,403,737 + -0.5 2,403,738 - -0.3 2,403,738 - -1.1 2,403,738 - +0.7 2,403,738 - +0.1 2,403,738 - +4.2 2,403,738 - -0.2 2,403,738 - +0.1 2,403,738 - -0.3 2,403,738 - +1.7 2,403,738 - +0.7 2,403,738 - +0.8
Or see this region's nucleotide sequence