Experiment: Def Neomycin 0.0008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2223 and Psest_2225 are separated by 532 nucleotides Psest_2225 and Psest_2226 are separated by 54 nucleotides Psest_2226 and Psest_2227 are separated by 40 nucleotides Psest_2227 and Psest_2228 overlap by 1 nucleotides
Psest_2223: Psest_2223 - cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases, at 2,373,246 to 2,373,995
_2223
Psest_2225: Psest_2225 - Diacylglycerol kinase, at 2,374,528 to 2,374,914
_2225
Psest_2226: Psest_2226 - Phosphoglycerol transferase and related proteins, alkaline phosphatase superfamily, at 2,374,969 to 2,377,005
_2226
Psest_2227: Psest_2227 - hypothetical protein, at 2,377,046 to 2,377,663
_2227
Psest_2228: Psest_2228 - hypothetical protein, at 2,377,663 to 2,378,958
_2228
Position (kb)
2374
2375
2376
2377
2378 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2374.013 kb on + strand at 2374.014 kb on - strand at 2374.051 kb on + strand at 2374.103 kb on + strand at 2374.103 kb on + strand at 2374.103 kb on + strand at 2374.103 kb on + strand at 2374.103 kb on + strand at 2374.103 kb on + strand at 2374.104 kb on - strand at 2374.104 kb on - strand at 2374.104 kb on - strand at 2374.104 kb on - strand at 2374.104 kb on - strand at 2374.104 kb on - strand at 2374.104 kb on - strand at 2374.106 kb on + strand at 2374.106 kb on + strand at 2374.143 kb on + strand at 2374.143 kb on + strand at 2374.161 kb on - strand at 2374.161 kb on - strand at 2374.291 kb on - strand at 2374.299 kb on + strand at 2374.299 kb on + strand at 2374.300 kb on - strand at 2374.300 kb on - strand at 2374.300 kb on - strand at 2374.305 kb on - strand at 2374.496 kb on + strand at 2374.497 kb on - strand at 2374.497 kb on - strand at 2374.497 kb on - strand at 2374.498 kb on + strand at 2374.498 kb on + strand at 2374.499 kb on - strand at 2374.499 kb on - strand at 2374.527 kb on + strand at 2374.528 kb on - strand at 2374.620 kb on + strand, within Psest_2225 at 2374.661 kb on + strand, within Psest_2225 at 2374.662 kb on - strand, within Psest_2225 at 2374.662 kb on - strand, within Psest_2225 at 2374.709 kb on + strand, within Psest_2225 at 2374.910 kb on - strand at 2374.991 kb on + strand at 2374.991 kb on + strand at 2375.194 kb on + strand, within Psest_2226 at 2375.194 kb on + strand, within Psest_2226 at 2375.195 kb on - strand, within Psest_2226 at 2375.195 kb on - strand, within Psest_2226 at 2375.195 kb on - strand, within Psest_2226 at 2375.546 kb on + strand, within Psest_2226 at 2375.547 kb on - strand, within Psest_2226 at 2375.547 kb on - strand, within Psest_2226 at 2375.547 kb on - strand, within Psest_2226 at 2375.666 kb on + strand, within Psest_2226 at 2375.682 kb on + strand, within Psest_2226 at 2375.705 kb on - strand, within Psest_2226 at 2375.786 kb on + strand, within Psest_2226 at 2375.786 kb on + strand, within Psest_2226 at 2375.786 kb on + strand, within Psest_2226 at 2375.786 kb on + strand, within Psest_2226 at 2375.786 kb on + strand, within Psest_2226 at 2375.787 kb on - strand, within Psest_2226 at 2375.787 kb on - strand, within Psest_2226 at 2375.787 kb on - strand, within Psest_2226 at 2375.861 kb on + strand, within Psest_2226 at 2375.861 kb on + strand, within Psest_2226 at 2375.861 kb on + strand, within Psest_2226 at 2375.861 kb on + strand, within Psest_2226 at 2375.861 kb on + strand, within Psest_2226 at 2375.861 kb on + strand, within Psest_2226 at 2375.862 kb on - strand, within Psest_2226 at 2375.862 kb on - strand, within Psest_2226 at 2375.862 kb on - strand, within Psest_2226 at 2375.862 kb on - strand, within Psest_2226 at 2375.862 kb on - strand, within Psest_2226 at 2375.862 kb on - strand, within Psest_2226 at 2375.864 kb on + strand, within Psest_2226 at 2375.864 kb on + strand, within Psest_2226 at 2375.864 kb on + strand, within Psest_2226 at 2375.865 kb on - strand, within Psest_2226 at 2375.865 kb on - strand, within Psest_2226 at 2375.865 kb on - strand, within Psest_2226 at 2375.865 kb on - strand, within Psest_2226 at 2375.947 kb on + strand, within Psest_2226 at 2375.947 kb on + strand, within Psest_2226 at 2375.948 kb on - strand, within Psest_2226 at 2376.009 kb on - strand, within Psest_2226 at 2376.240 kb on - strand, within Psest_2226 at 2376.240 kb on - strand, within Psest_2226 at 2376.296 kb on + strand, within Psest_2226 at 2376.662 kb on + strand, within Psest_2226 at 2376.663 kb on - strand, within Psest_2226 at 2376.663 kb on - strand, within Psest_2226 at 2376.668 kb on + strand, within Psest_2226 at 2376.669 kb on - strand, within Psest_2226 at 2376.689 kb on + strand, within Psest_2226 at 2376.689 kb on + strand, within Psest_2226 at 2376.689 kb on + strand, within Psest_2226 at 2376.689 kb on + strand, within Psest_2226 at 2376.689 kb on + strand, within Psest_2226 at 2376.690 kb on - strand, within Psest_2226 at 2376.690 kb on - strand, within Psest_2226 at 2376.690 kb on - strand, within Psest_2226 at 2376.690 kb on - strand, within Psest_2226 at 2376.881 kb on - strand at 2376.975 kb on - strand at 2377.019 kb on + strand at 2377.020 kb on - strand at 2377.031 kb on - strand at 2377.109 kb on - strand, within Psest_2227 at 2377.109 kb on - strand, within Psest_2227 at 2377.176 kb on + strand, within Psest_2227 at 2377.177 kb on - strand, within Psest_2227 at 2377.177 kb on - strand, within Psest_2227 at 2377.177 kb on - strand, within Psest_2227 at 2377.177 kb on - strand, within Psest_2227 at 2377.177 kb on - strand, within Psest_2227 at 2377.180 kb on + strand, within Psest_2227 at 2377.509 kb on + strand, within Psest_2227 at 2377.509 kb on + strand, within Psest_2227 at 2377.509 kb on + strand, within Psest_2227 at 2377.509 kb on + strand, within Psest_2227 at 2377.509 kb on + strand, within Psest_2227 at 2377.509 kb on + strand, within Psest_2227 at 2377.510 kb on - strand, within Psest_2227 at 2377.510 kb on - strand, within Psest_2227 at 2377.510 kb on - strand, within Psest_2227 at 2377.510 kb on - strand, within Psest_2227 at 2377.510 kb on - strand, within Psest_2227 at 2377.510 kb on - strand, within Psest_2227 at 2377.510 kb on - strand, within Psest_2227 at 2377.510 kb on - strand, within Psest_2227 at 2377.562 kb on + strand, within Psest_2227 at 2377.563 kb on - strand, within Psest_2227 at 2377.730 kb on + strand at 2377.770 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Neomycin 0.0008 mg/ml remove 2,374,013 + -2.5 2,374,014 - -0.9 2,374,051 + -0.9 2,374,103 + -0.1 2,374,103 + +0.7 2,374,103 + -1.3 2,374,103 + +0.5 2,374,103 + +0.8 2,374,103 + +0.1 2,374,104 - -0.9 2,374,104 - -3.4 2,374,104 - -0.3 2,374,104 - -0.1 2,374,104 - -0.4 2,374,104 - -0.3 2,374,104 - -0.3 2,374,106 + +0.9 2,374,106 + +0.1 2,374,143 + -0.7 2,374,143 + +0.9 2,374,161 - +0.1 2,374,161 - +0.7 2,374,291 - -1.4 2,374,299 + -0.4 2,374,299 + -0.2 2,374,300 - +1.3 2,374,300 - -1.4 2,374,300 - -0.7 2,374,305 - +2.9 2,374,496 + -1.0 2,374,497 - +0.4 2,374,497 - +3.0 2,374,497 - +1.4 2,374,498 + +0.5 2,374,498 + -2.3 2,374,499 - -2.2 2,374,499 - +2.2 2,374,527 + -1.4 2,374,528 - -0.7 2,374,620 + Psest_2225 0.24 +1.4 2,374,661 + Psest_2225 0.34 -0.5 2,374,662 - Psest_2225 0.35 +0.3 2,374,662 - Psest_2225 0.35 -0.7 2,374,709 + Psest_2225 0.47 -1.7 2,374,910 - -0.3 2,374,991 + +1.0 2,374,991 + +1.6 2,375,194 + Psest_2226 0.11 +0.7 2,375,194 + Psest_2226 0.11 -0.3 2,375,195 - Psest_2226 0.11 -0.3 2,375,195 - Psest_2226 0.11 -1.3 2,375,195 - Psest_2226 0.11 -0.3 2,375,546 + Psest_2226 0.28 +0.3 2,375,547 - Psest_2226 0.28 -0.6 2,375,547 - Psest_2226 0.28 -0.6 2,375,547 - Psest_2226 0.28 +0.0 2,375,666 + Psest_2226 0.34 -1.3 2,375,682 + Psest_2226 0.35 +0.0 2,375,705 - Psest_2226 0.36 -0.9 2,375,786 + Psest_2226 0.40 -0.6 2,375,786 + Psest_2226 0.40 -1.1 2,375,786 + Psest_2226 0.40 -1.1 2,375,786 + Psest_2226 0.40 +1.6 2,375,786 + Psest_2226 0.40 -2.0 2,375,787 - Psest_2226 0.40 +1.9 2,375,787 - Psest_2226 0.40 -0.9 2,375,787 - Psest_2226 0.40 +0.0 2,375,861 + Psest_2226 0.44 +1.0 2,375,861 + Psest_2226 0.44 -0.6 2,375,861 + Psest_2226 0.44 -0.4 2,375,861 + Psest_2226 0.44 -1.9 2,375,861 + Psest_2226 0.44 +0.4 2,375,861 + Psest_2226 0.44 +0.0 2,375,862 - Psest_2226 0.44 -1.5 2,375,862 - Psest_2226 0.44 -1.9 2,375,862 - Psest_2226 0.44 -1.0 2,375,862 - Psest_2226 0.44 +0.9 2,375,862 - Psest_2226 0.44 -0.6 2,375,862 - Psest_2226 0.44 -2.3 2,375,864 + Psest_2226 0.44 +1.5 2,375,864 + Psest_2226 0.44 -1.9 2,375,864 + Psest_2226 0.44 -0.7 2,375,865 - Psest_2226 0.44 -0.9 2,375,865 - Psest_2226 0.44 -0.7 2,375,865 - Psest_2226 0.44 +1.1 2,375,865 - Psest_2226 0.44 +3.3 2,375,947 + Psest_2226 0.48 +0.7 2,375,947 + Psest_2226 0.48 -3.4 2,375,948 - Psest_2226 0.48 +0.7 2,376,009 - Psest_2226 0.51 -0.4 2,376,240 - Psest_2226 0.62 -1.5 2,376,240 - Psest_2226 0.62 -0.6 2,376,296 + Psest_2226 0.65 +1.2 2,376,662 + Psest_2226 0.83 -1.3 2,376,663 - Psest_2226 0.83 +2.5 2,376,663 - Psest_2226 0.83 +0.4 2,376,668 + Psest_2226 0.83 -1.2 2,376,669 - Psest_2226 0.83 -0.2 2,376,689 + Psest_2226 0.84 -0.3 2,376,689 + Psest_2226 0.84 -1.4 2,376,689 + Psest_2226 0.84 +0.3 2,376,689 + Psest_2226 0.84 +0.1 2,376,689 + Psest_2226 0.84 +0.5 2,376,690 - Psest_2226 0.84 -0.1 2,376,690 - Psest_2226 0.84 +0.6 2,376,690 - Psest_2226 0.84 -0.5 2,376,690 - Psest_2226 0.84 -1.9 2,376,881 - -0.6 2,376,975 - +2.4 2,377,019 + +0.4 2,377,020 - -1.1 2,377,031 - -0.0 2,377,109 - Psest_2227 0.10 -0.7 2,377,109 - Psest_2227 0.10 -0.9 2,377,176 + Psest_2227 0.21 +1.7 2,377,177 - Psest_2227 0.21 +2.0 2,377,177 - Psest_2227 0.21 -2.7 2,377,177 - Psest_2227 0.21 -1.8 2,377,177 - Psest_2227 0.21 -0.3 2,377,177 - Psest_2227 0.21 +1.8 2,377,180 + Psest_2227 0.22 -0.7 2,377,509 + Psest_2227 0.75 -0.9 2,377,509 + Psest_2227 0.75 +0.8 2,377,509 + Psest_2227 0.75 -0.6 2,377,509 + Psest_2227 0.75 -2.4 2,377,509 + Psest_2227 0.75 -1.4 2,377,509 + Psest_2227 0.75 -1.6 2,377,510 - Psest_2227 0.75 +0.5 2,377,510 - Psest_2227 0.75 -1.9 2,377,510 - Psest_2227 0.75 +3.0 2,377,510 - Psest_2227 0.75 +0.4 2,377,510 - Psest_2227 0.75 +2.1 2,377,510 - Psest_2227 0.75 +0.8 2,377,510 - Psest_2227 0.75 -0.1 2,377,510 - Psest_2227 0.75 -0.9 2,377,562 + Psest_2227 0.83 +0.3 2,377,563 - Psest_2227 0.84 +1.7 2,377,730 + -1.1 2,377,770 - +0.1
Or see this region's nucleotide sequence