Experiment: Def Neomycin 0.0008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1750 and Psest_1751 are separated by 134 nucleotides Psest_1751 and Psest_1752 overlap by 20 nucleotides Psest_1752 and Psest_1753 overlap by 4 nucleotides Psest_1753 and Psest_1754 are separated by 135 nucleotides Psest_1754 and Psest_1755 overlap by 4 nucleotides
Psest_1750: Psest_1750 - Protein of unknown function (DUF2802)., at 1,854,588 to 1,855,001
_1750
Psest_1751: Psest_1751 - Rhodanese-related sulfurtransferase, at 1,855,136 to 1,855,579
_1751
Psest_1752: Psest_1752 - Uncharacterized homolog of the cytoplasmic domain of flagellar protein FhlB, at 1,855,560 to 1,855,892
_1752
Psest_1753: Psest_1753 - Flagellar hook-length control protein FliK., at 1,855,889 to 1,857,418
_1753
Psest_1754: Psest_1754 - heme ABC exporter, ATP-binding protein CcmA, at 1,857,554 to 1,858,189
_1754
Psest_1755: Psest_1755 - heme exporter protein CcmB, at 1,858,186 to 1,858,857
_1755
Position (kb) 1855
1856
1857
1858 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1854.911 kb on + strand, within Psest_1750 at 1855.186 kb on - strand, within Psest_1751 at 1855.403 kb on + strand, within Psest_1751 at 1855.403 kb on + strand, within Psest_1751 at 1855.403 kb on + strand, within Psest_1751 at 1855.404 kb on - strand, within Psest_1751 at 1855.404 kb on - strand, within Psest_1751 at 1855.419 kb on + strand, within Psest_1751 at 1855.419 kb on + strand, within Psest_1751 at 1855.419 kb on + strand, within Psest_1751 at 1855.419 kb on + strand, within Psest_1751 at 1855.419 kb on + strand, within Psest_1751 at 1855.419 kb on + strand, within Psest_1751 at 1855.420 kb on - strand, within Psest_1751 at 1855.420 kb on - strand, within Psest_1751 at 1855.420 kb on - strand, within Psest_1751 at 1855.467 kb on - strand, within Psest_1751 at 1855.481 kb on + strand, within Psest_1751 at 1855.482 kb on - strand, within Psest_1751 at 1855.496 kb on + strand, within Psest_1751 at 1855.497 kb on - strand, within Psest_1751 at 1855.512 kb on + strand, within Psest_1751 at 1855.513 kb on - strand, within Psest_1751 at 1855.652 kb on - strand, within Psest_1752 at 1855.714 kb on - strand, within Psest_1752 at 1856.263 kb on + strand, within Psest_1753 at 1856.264 kb on - strand, within Psest_1753 at 1856.585 kb on + strand, within Psest_1753 at 1856.585 kb on + strand, within Psest_1753 at 1856.639 kb on + strand, within Psest_1753 at 1856.640 kb on - strand, within Psest_1753 at 1857.159 kb on + strand, within Psest_1753 at 1857.159 kb on + strand, within Psest_1753 at 1857.159 kb on + strand, within Psest_1753 at 1857.160 kb on - strand, within Psest_1753 at 1857.436 kb on + strand at 1857.437 kb on - strand at 1857.437 kb on - strand at 1857.481 kb on + strand at 1857.481 kb on + strand at 1857.481 kb on + strand at 1857.481 kb on + strand at 1857.481 kb on + strand at 1857.482 kb on - strand at 1857.482 kb on - strand at 1857.482 kb on - strand at 1857.482 kb on - strand at 1857.482 kb on - strand at 1857.483 kb on + strand at 1857.514 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Neomycin 0.0008 mg/ml remove 1,854,911 + Psest_1750 0.78 -1.4 1,855,186 - Psest_1751 0.11 +1.7 1,855,403 + Psest_1751 0.60 -3.0 1,855,403 + Psest_1751 0.60 +1.2 1,855,403 + Psest_1751 0.60 -1.2 1,855,404 - Psest_1751 0.60 +0.9 1,855,404 - Psest_1751 0.60 -0.4 1,855,419 + Psest_1751 0.64 -0.0 1,855,419 + Psest_1751 0.64 +0.1 1,855,419 + Psest_1751 0.64 +0.8 1,855,419 + Psest_1751 0.64 +1.3 1,855,419 + Psest_1751 0.64 +0.1 1,855,419 + Psest_1751 0.64 +0.9 1,855,420 - Psest_1751 0.64 -1.2 1,855,420 - Psest_1751 0.64 +0.6 1,855,420 - Psest_1751 0.64 -1.3 1,855,467 - Psest_1751 0.75 -2.0 1,855,481 + Psest_1751 0.78 -0.1 1,855,482 - Psest_1751 0.78 +0.5 1,855,496 + Psest_1751 0.81 -0.7 1,855,497 - Psest_1751 0.81 +0.3 1,855,512 + Psest_1751 0.85 +1.8 1,855,513 - Psest_1751 0.85 +0.7 1,855,652 - Psest_1752 0.28 +0.2 1,855,714 - Psest_1752 0.46 -2.3 1,856,263 + Psest_1753 0.24 -0.3 1,856,264 - Psest_1753 0.25 -1.0 1,856,585 + Psest_1753 0.45 +0.1 1,856,585 + Psest_1753 0.45 -1.5 1,856,639 + Psest_1753 0.49 -1.0 1,856,640 - Psest_1753 0.49 -2.1 1,857,159 + Psest_1753 0.83 +0.2 1,857,159 + Psest_1753 0.83 +0.6 1,857,159 + Psest_1753 0.83 -0.1 1,857,160 - Psest_1753 0.83 -2.5 1,857,436 + -0.4 1,857,437 - +0.3 1,857,437 - +0.4 1,857,481 + -0.4 1,857,481 + +0.1 1,857,481 + -0.9 1,857,481 + -0.0 1,857,481 + -0.6 1,857,482 - +1.3 1,857,482 - +0.2 1,857,482 - +1.5 1,857,482 - +2.1 1,857,482 - +2.0 1,857,483 + -2.2 1,857,514 + -0.7
Or see this region's nucleotide sequence