Experiment: Def Neomycin 0.0008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1447 and Psest_1448 are separated by 75 nucleotides Psest_1448 and Psest_1449 are separated by 80 nucleotides Psest_1449 and Psest_1450 are separated by 8 nucleotides
Psest_1447: Psest_1447 - Putative threonine efflux protein, at 1,556,137 to 1,556,772
_1447
Psest_1448: Psest_1448 - Uncharacterized protein conserved in bacteria, at 1,556,848 to 1,557,303
_1448
Psest_1449: Psest_1449 - Short-chain dehydrogenases of various substrate specificities, at 1,557,384 to 1,558,358
_1449
Psest_1450: Psest_1450 - Transcriptional regulator, at 1,558,367 to 1,559,293
_1450
Position (kb)
1557
1558
1559 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1556.384 kb on - strand, within Psest_1447 at 1556.533 kb on - strand, within Psest_1447 at 1556.602 kb on - strand, within Psest_1447 at 1556.847 kb on + strand at 1556.847 kb on + strand at 1556.847 kb on + strand at 1556.847 kb on + strand at 1556.847 kb on + strand at 1556.848 kb on - strand at 1556.848 kb on - strand at 1556.848 kb on - strand at 1556.848 kb on - strand at 1556.848 kb on - strand at 1556.848 kb on - strand at 1556.848 kb on - strand at 1556.848 kb on - strand at 1556.848 kb on - strand at 1557.053 kb on - strand, within Psest_1448 at 1557.105 kb on + strand, within Psest_1448 at 1557.105 kb on + strand, within Psest_1448 at 1557.105 kb on + strand, within Psest_1448 at 1557.106 kb on - strand, within Psest_1448 at 1557.106 kb on - strand, within Psest_1448 at 1557.109 kb on + strand, within Psest_1448 at 1557.109 kb on + strand, within Psest_1448 at 1557.109 kb on + strand, within Psest_1448 at 1557.109 kb on + strand, within Psest_1448 at 1557.110 kb on - strand, within Psest_1448 at 1557.110 kb on - strand, within Psest_1448 at 1557.110 kb on - strand, within Psest_1448 at 1557.110 kb on - strand, within Psest_1448 at 1557.110 kb on - strand, within Psest_1448 at 1557.110 kb on - strand, within Psest_1448 at 1557.110 kb on - strand, within Psest_1448 at 1557.110 kb on - strand, within Psest_1448 at 1557.110 kb on - strand, within Psest_1448 at 1557.110 kb on - strand, within Psest_1448 at 1557.110 kb on - strand, within Psest_1448 at 1557.110 kb on - strand, within Psest_1448 at 1557.110 kb on - strand, within Psest_1448 at 1557.110 kb on - strand, within Psest_1448 at 1557.110 kb on - strand, within Psest_1448 at 1557.110 kb on - strand, within Psest_1448 at 1557.110 kb on - strand, within Psest_1448 at 1557.110 kb on - strand, within Psest_1448 at 1557.110 kb on - strand, within Psest_1448 at 1557.110 kb on - strand, within Psest_1448 at 1557.197 kb on - strand, within Psest_1448 at 1557.256 kb on + strand, within Psest_1448 at 1557.256 kb on + strand, within Psest_1448 at 1557.256 kb on + strand, within Psest_1448 at 1557.257 kb on - strand, within Psest_1448 at 1557.352 kb on + strand at 1557.352 kb on + strand at 1557.352 kb on + strand at 1557.356 kb on + strand at 1557.356 kb on + strand at 1557.357 kb on - strand at 1557.357 kb on - strand at 1557.357 kb on - strand at 1557.357 kb on - strand at 1557.418 kb on + strand at 1557.745 kb on - strand, within Psest_1449 at 1557.774 kb on + strand, within Psest_1449 at 1557.775 kb on - strand, within Psest_1449 at 1557.932 kb on + strand, within Psest_1449 at 1557.933 kb on - strand, within Psest_1449 at 1557.933 kb on - strand, within Psest_1449 at 1557.933 kb on - strand, within Psest_1449 at 1557.933 kb on - strand, within Psest_1449 at 1557.990 kb on + strand, within Psest_1449 at 1557.990 kb on + strand, within Psest_1449 at 1557.990 kb on + strand, within Psest_1449 at 1557.990 kb on + strand, within Psest_1449 at 1557.990 kb on + strand, within Psest_1449 at 1557.990 kb on + strand, within Psest_1449 at 1557.990 kb on + strand, within Psest_1449 at 1557.990 kb on + strand, within Psest_1449 at 1557.990 kb on + strand, within Psest_1449 at 1557.991 kb on - strand at 1557.991 kb on - strand, within Psest_1449 at 1557.991 kb on - strand, within Psest_1449 at 1557.991 kb on - strand, within Psest_1449 at 1557.991 kb on - strand, within Psest_1449 at 1557.991 kb on - strand, within Psest_1449 at 1557.991 kb on - strand, within Psest_1449 at 1557.991 kb on - strand, within Psest_1449 at 1557.991 kb on - strand, within Psest_1449 at 1558.082 kb on + strand, within Psest_1449 at 1559.302 kb on - strand at 1559.302 kb on - strand at 1559.302 kb on - strand at 1559.302 kb on - strand at 1559.302 kb on - strand at 1559.302 kb on - strand at 1559.302 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Neomycin 0.0008 mg/ml remove 1,556,384 - Psest_1447 0.39 -0.9 1,556,533 - Psest_1447 0.62 -0.3 1,556,602 - Psest_1447 0.73 +0.3 1,556,847 + +1.9 1,556,847 + +0.3 1,556,847 + +1.0 1,556,847 + -0.3 1,556,847 + +0.4 1,556,848 - -2.4 1,556,848 - -0.2 1,556,848 - -1.4 1,556,848 - +0.2 1,556,848 - +0.7 1,556,848 - -0.1 1,556,848 - -0.5 1,556,848 - -2.6 1,556,848 - -0.3 1,557,053 - Psest_1448 0.45 -1.2 1,557,105 + Psest_1448 0.56 -1.9 1,557,105 + Psest_1448 0.56 +0.1 1,557,105 + Psest_1448 0.56 +2.7 1,557,106 - Psest_1448 0.57 -1.0 1,557,106 - Psest_1448 0.57 +0.7 1,557,109 + Psest_1448 0.57 -1.1 1,557,109 + Psest_1448 0.57 -0.3 1,557,109 + Psest_1448 0.57 +0.3 1,557,109 + Psest_1448 0.57 +1.3 1,557,110 - Psest_1448 0.57 -2.0 1,557,110 - Psest_1448 0.57 +0.8 1,557,110 - Psest_1448 0.57 -0.3 1,557,110 - Psest_1448 0.57 +0.5 1,557,110 - Psest_1448 0.57 +0.7 1,557,110 - Psest_1448 0.57 -0.9 1,557,110 - Psest_1448 0.57 -1.1 1,557,110 - Psest_1448 0.57 -2.3 1,557,110 - Psest_1448 0.57 +0.5 1,557,110 - Psest_1448 0.57 -0.6 1,557,110 - Psest_1448 0.57 -0.1 1,557,110 - Psest_1448 0.57 -1.3 1,557,110 - Psest_1448 0.57 -1.7 1,557,110 - Psest_1448 0.57 +0.5 1,557,110 - Psest_1448 0.57 -0.8 1,557,110 - Psest_1448 0.57 +0.8 1,557,110 - Psest_1448 0.57 +0.9 1,557,110 - Psest_1448 0.57 +1.6 1,557,110 - Psest_1448 0.57 +0.0 1,557,110 - Psest_1448 0.57 +3.5 1,557,197 - Psest_1448 0.77 -1.3 1,557,256 + Psest_1448 0.89 +0.1 1,557,256 + Psest_1448 0.89 +0.7 1,557,256 + Psest_1448 0.89 +3.8 1,557,257 - Psest_1448 0.90 -0.3 1,557,352 + -1.4 1,557,352 + +1.0 1,557,352 + -0.3 1,557,356 + +0.1 1,557,356 + +3.0 1,557,357 - -1.3 1,557,357 - +1.4 1,557,357 - +3.0 1,557,357 - +2.3 1,557,418 + +1.7 1,557,745 - Psest_1449 0.37 +1.1 1,557,774 + Psest_1449 0.40 +0.8 1,557,775 - Psest_1449 0.40 +1.6 1,557,932 + Psest_1449 0.56 +0.7 1,557,933 - Psest_1449 0.56 +0.1 1,557,933 - Psest_1449 0.56 +1.7 1,557,933 - Psest_1449 0.56 +0.8 1,557,933 - Psest_1449 0.56 +1.7 1,557,990 + Psest_1449 0.62 -0.3 1,557,990 + Psest_1449 0.62 +0.3 1,557,990 + Psest_1449 0.62 +0.7 1,557,990 + Psest_1449 0.62 -0.9 1,557,990 + Psest_1449 0.62 +0.5 1,557,990 + Psest_1449 0.62 +1.7 1,557,990 + Psest_1449 0.62 +0.4 1,557,990 + Psest_1449 0.62 +2.4 1,557,990 + Psest_1449 0.62 +1.2 1,557,991 - +0.1 1,557,991 - Psest_1449 0.62 +2.0 1,557,991 - Psest_1449 0.62 +0.9 1,557,991 - Psest_1449 0.62 +0.7 1,557,991 - Psest_1449 0.62 +0.4 1,557,991 - Psest_1449 0.62 -0.9 1,557,991 - Psest_1449 0.62 +0.7 1,557,991 - Psest_1449 0.62 +0.5 1,557,991 - Psest_1449 0.62 -0.1 1,558,082 + Psest_1449 0.72 +0.6 1,559,302 - -1.9 1,559,302 - -2.8 1,559,302 - +1.7 1,559,302 - +0.7 1,559,302 - -2.1 1,559,302 - -1.6 1,559,302 - -0.3
Or see this region's nucleotide sequence