Experiment: Def Neomycin 0.0008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1322 and Psest_1323 overlap by 4 nucleotides Psest_1323 and Psest_1324 are separated by 121 nucleotides
Psest_1322: Psest_1322 - 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases, at 1,422,976 to 1,423,863
_1322
Psest_1323: Psest_1323 - Hydroxypyruvate isomerase, at 1,423,860 to 1,424,642
_1323
Psest_1324: Psest_1324 - Arabinose efflux permease, at 1,424,764 to 1,426,110
_1324
Position (kb)
1423
1424
1425 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1423.127 kb on + strand, within Psest_1322 at 1423.359 kb on + strand, within Psest_1322 at 1423.467 kb on + strand, within Psest_1322 at 1423.843 kb on + strand at 1423.843 kb on + strand at 1423.843 kb on + strand at 1423.843 kb on + strand at 1423.844 kb on - strand at 1423.844 kb on - strand at 1423.844 kb on - strand at 1423.844 kb on - strand at 1424.116 kb on + strand, within Psest_1323 at 1424.133 kb on + strand, within Psest_1323 at 1424.133 kb on + strand, within Psest_1323 at 1424.133 kb on + strand, within Psest_1323 at 1424.133 kb on + strand, within Psest_1323 at 1424.133 kb on + strand, within Psest_1323 at 1424.133 kb on + strand, within Psest_1323 at 1424.134 kb on - strand, within Psest_1323 at 1424.134 kb on - strand, within Psest_1323 at 1424.395 kb on - strand, within Psest_1323 at 1424.400 kb on - strand, within Psest_1323 at 1424.578 kb on - strand at 1424.699 kb on + strand at 1424.699 kb on + strand at 1424.699 kb on + strand at 1424.699 kb on + strand at 1424.700 kb on - strand at 1424.700 kb on - strand at 1424.739 kb on + strand at 1424.739 kb on + strand at 1424.739 kb on + strand at 1424.739 kb on + strand at 1424.740 kb on - strand at 1424.740 kb on - strand at 1424.740 kb on - strand at 1424.740 kb on - strand at 1424.741 kb on + strand at 1424.741 kb on + strand at 1424.741 kb on + strand at 1424.741 kb on + strand at 1424.741 kb on + strand at 1424.741 kb on + strand at 1424.742 kb on - strand at 1424.742 kb on - strand at 1424.742 kb on - strand at 1424.742 kb on - strand at 1424.742 kb on - strand at 1424.742 kb on - strand at 1424.941 kb on + strand, within Psest_1324 at 1424.941 kb on + strand, within Psest_1324 at 1424.942 kb on - strand, within Psest_1324 at 1425.460 kb on + strand, within Psest_1324 at 1425.460 kb on + strand, within Psest_1324 at 1425.460 kb on + strand, within Psest_1324 at 1425.460 kb on + strand, within Psest_1324 at 1425.460 kb on + strand, within Psest_1324 at 1425.460 kb on + strand, within Psest_1324 at 1425.460 kb on + strand, within Psest_1324 at 1425.460 kb on + strand, within Psest_1324 at 1425.460 kb on + strand, within Psest_1324 at 1425.461 kb on - strand, within Psest_1324 at 1425.461 kb on - strand, within Psest_1324 at 1425.461 kb on - strand, within Psest_1324 at 1425.461 kb on - strand, within Psest_1324 at 1425.469 kb on - strand, within Psest_1324
Per-strain Table
Position Strand Gene LocusTag Fraction Def Neomycin 0.0008 mg/ml remove 1,423,127 + Psest_1322 0.17 +0.1 1,423,359 + Psest_1322 0.43 -1.2 1,423,467 + Psest_1322 0.55 +0.2 1,423,843 + +0.2 1,423,843 + +1.0 1,423,843 + +0.1 1,423,843 + -0.8 1,423,844 - +0.5 1,423,844 - +0.6 1,423,844 - -0.6 1,423,844 - -1.1 1,424,116 + Psest_1323 0.33 +1.0 1,424,133 + Psest_1323 0.35 -2.5 1,424,133 + Psest_1323 0.35 -2.6 1,424,133 + Psest_1323 0.35 +0.6 1,424,133 + Psest_1323 0.35 -1.5 1,424,133 + Psest_1323 0.35 +0.1 1,424,133 + Psest_1323 0.35 -0.9 1,424,134 - Psest_1323 0.35 -1.1 1,424,134 - Psest_1323 0.35 -1.9 1,424,395 - Psest_1323 0.68 -0.4 1,424,400 - Psest_1323 0.69 -2.6 1,424,578 - +0.5 1,424,699 + +0.7 1,424,699 + -0.2 1,424,699 + +1.4 1,424,699 + +1.3 1,424,700 - +0.7 1,424,700 - +0.7 1,424,739 + -1.5 1,424,739 + -1.2 1,424,739 + -0.3 1,424,739 + -0.4 1,424,740 - -0.9 1,424,740 - +0.3 1,424,740 - +0.1 1,424,740 - +1.2 1,424,741 + +1.2 1,424,741 + +0.8 1,424,741 + -1.3 1,424,741 + +0.6 1,424,741 + +1.7 1,424,741 + -0.9 1,424,742 - +0.4 1,424,742 - -0.5 1,424,742 - -3.0 1,424,742 - -2.0 1,424,742 - -0.9 1,424,742 - +1.1 1,424,941 + Psest_1324 0.13 -1.0 1,424,941 + Psest_1324 0.13 -0.3 1,424,942 - Psest_1324 0.13 -1.7 1,425,460 + Psest_1324 0.52 +0.1 1,425,460 + Psest_1324 0.52 -0.1 1,425,460 + Psest_1324 0.52 -1.5 1,425,460 + Psest_1324 0.52 -0.8 1,425,460 + Psest_1324 0.52 -2.0 1,425,460 + Psest_1324 0.52 +0.7 1,425,460 + Psest_1324 0.52 +1.1 1,425,460 + Psest_1324 0.52 +0.1 1,425,460 + Psest_1324 0.52 +1.0 1,425,461 - Psest_1324 0.52 +0.2 1,425,461 - Psest_1324 0.52 +0.0 1,425,461 - Psest_1324 0.52 +0.8 1,425,461 - Psest_1324 0.52 +1.3 1,425,469 - Psest_1324 0.52 +0.7
Or see this region's nucleotide sequence