Experiment: Def Neomycin 0.0008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1318 and Psest_1319 overlap by 7 nucleotides Psest_1319 and Psest_1320 are separated by 43 nucleotides Psest_1320 and Psest_1321 are separated by 500 nucleotides
Psest_1318: Psest_1318 - ABC-type phosphate/phosphonate transport system, permease component, at 1,418,832 to 1,419,665
_1318
Psest_1319: Psest_1319 - ABC-type phosphate/phosphonate transport system, ATPase component, at 1,419,659 to 1,420,489
_1319
Psest_1320: Psest_1320 - phosphate/phosphite/phosphonate ABC transporters, periplasmic binding protein, at 1,420,533 to 1,421,384
_1320
Psest_1321: Psest_1321 - undecaprenyl-diphosphatase UppP, at 1,421,885 to 1,422,697
_1321
Position (kb)
1420
1421
1422 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1419.735 kb on + strand at 1419.735 kb on + strand at 1419.736 kb on - strand at 1419.736 kb on - strand at 1419.736 kb on - strand at 1419.962 kb on + strand, within Psest_1319 at 1419.979 kb on - strand, within Psest_1319 at 1420.041 kb on + strand, within Psest_1319 at 1420.041 kb on + strand, within Psest_1319 at 1420.041 kb on + strand, within Psest_1319 at 1420.041 kb on + strand, within Psest_1319 at 1420.042 kb on - strand, within Psest_1319 at 1420.042 kb on - strand, within Psest_1319 at 1420.042 kb on - strand, within Psest_1319 at 1420.042 kb on - strand, within Psest_1319 at 1420.042 kb on - strand, within Psest_1319 at 1420.042 kb on - strand, within Psest_1319 at 1420.042 kb on - strand, within Psest_1319 at 1420.042 kb on - strand, within Psest_1319 at 1420.042 kb on - strand, within Psest_1319 at 1420.191 kb on - strand, within Psest_1319 at 1420.191 kb on - strand, within Psest_1319 at 1420.433 kb on - strand at 1420.729 kb on + strand, within Psest_1320 at 1420.735 kb on + strand, within Psest_1320 at 1420.735 kb on + strand, within Psest_1320 at 1420.736 kb on - strand, within Psest_1320 at 1420.736 kb on - strand, within Psest_1320 at 1420.736 kb on - strand, within Psest_1320 at 1420.903 kb on + strand, within Psest_1320 at 1420.904 kb on - strand, within Psest_1320 at 1420.904 kb on - strand, within Psest_1320 at 1421.076 kb on + strand, within Psest_1320 at 1421.076 kb on + strand, within Psest_1320 at 1421.185 kb on + strand, within Psest_1320 at 1421.185 kb on + strand, within Psest_1320 at 1421.237 kb on - strand, within Psest_1320 at 1421.333 kb on + strand at 1421.333 kb on + strand at 1421.333 kb on + strand at 1421.334 kb on - strand at 1421.405 kb on - strand at 1421.427 kb on + strand at 1421.427 kb on + strand at 1421.427 kb on + strand at 1421.508 kb on + strand at 1421.508 kb on + strand at 1421.509 kb on - strand at 1421.547 kb on + strand at 1421.711 kb on + strand at 1421.711 kb on + strand at 1421.712 kb on - strand at 1421.712 kb on - strand at 1421.712 kb on - strand at 1421.716 kb on - strand at 1421.716 kb on - strand at 1421.754 kb on + strand at 1421.901 kb on - strand at 1421.934 kb on + strand at 1421.935 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Neomycin 0.0008 mg/ml remove 1,419,735 + -1.7 1,419,735 + +0.6 1,419,736 - +1.8 1,419,736 - -1.5 1,419,736 - +0.5 1,419,962 + Psest_1319 0.36 -0.5 1,419,979 - Psest_1319 0.39 +0.6 1,420,041 + Psest_1319 0.46 -0.5 1,420,041 + Psest_1319 0.46 +1.9 1,420,041 + Psest_1319 0.46 +1.6 1,420,041 + Psest_1319 0.46 +0.7 1,420,042 - Psest_1319 0.46 +0.4 1,420,042 - Psest_1319 0.46 -1.3 1,420,042 - Psest_1319 0.46 +0.7 1,420,042 - Psest_1319 0.46 -0.5 1,420,042 - Psest_1319 0.46 -0.6 1,420,042 - Psest_1319 0.46 +0.7 1,420,042 - Psest_1319 0.46 +0.2 1,420,042 - Psest_1319 0.46 +0.9 1,420,042 - Psest_1319 0.46 +0.8 1,420,191 - Psest_1319 0.64 +1.3 1,420,191 - Psest_1319 0.64 -0.3 1,420,433 - -0.3 1,420,729 + Psest_1320 0.23 -0.0 1,420,735 + Psest_1320 0.24 +0.4 1,420,735 + Psest_1320 0.24 -0.6 1,420,736 - Psest_1320 0.24 -0.3 1,420,736 - Psest_1320 0.24 +0.4 1,420,736 - Psest_1320 0.24 +1.6 1,420,903 + Psest_1320 0.43 +1.2 1,420,904 - Psest_1320 0.44 +2.3 1,420,904 - Psest_1320 0.44 +2.2 1,421,076 + Psest_1320 0.64 +1.1 1,421,076 + Psest_1320 0.64 -2.2 1,421,185 + Psest_1320 0.77 +0.5 1,421,185 + Psest_1320 0.77 -2.0 1,421,237 - Psest_1320 0.83 +0.5 1,421,333 + +1.3 1,421,333 + +1.3 1,421,333 + -1.5 1,421,334 - -0.9 1,421,405 - +2.9 1,421,427 + -0.9 1,421,427 + -0.0 1,421,427 + -3.5 1,421,508 + -0.6 1,421,508 + +1.7 1,421,509 - +0.1 1,421,547 + -2.2 1,421,711 + -0.6 1,421,711 + -1.5 1,421,712 - +1.5 1,421,712 - -0.6 1,421,712 - -1.1 1,421,716 - -1.0 1,421,716 - -0.3 1,421,754 + -0.2 1,421,901 - -0.9 1,421,934 + -0.4 1,421,935 - -0.6
Or see this region's nucleotide sequence