Experiment: Def Neomycin 0.0008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1158 and Psest_1159 are separated by 166 nucleotides Psest_1159 and Psest_1160 are separated by 169 nucleotides Psest_1160 and Psest_1161 are separated by 1 nucleotides
Psest_1158: Psest_1158 - Predicted thiol oxidoreductase, at 1,258,095 to 1,259,513
_1158
Psest_1159: Psest_1159 - Uncharacterized iron-regulated protein, at 1,259,680 to 1,261,014
_1159
Psest_1160: Psest_1160 - Uncharacterized conserved protein, at 1,261,184 to 1,261,756
_1160
Psest_1161: Psest_1161 - E3 Ubiquitin ligase., at 1,261,758 to 1,262,651
_1161
Position (kb)
1259
1260
1261
1262 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1258.956 kb on + strand, within Psest_1158 at 1258.957 kb on - strand, within Psest_1158 at 1259.013 kb on + strand, within Psest_1158 at 1259.013 kb on + strand, within Psest_1158 at 1259.014 kb on - strand, within Psest_1158 at 1259.014 kb on - strand, within Psest_1158 at 1259.014 kb on - strand, within Psest_1158 at 1259.014 kb on - strand, within Psest_1158 at 1259.014 kb on - strand, within Psest_1158 at 1259.014 kb on - strand, within Psest_1158 at 1259.014 kb on - strand, within Psest_1158 at 1259.014 kb on - strand, within Psest_1158 at 1259.014 kb on - strand, within Psest_1158 at 1259.202 kb on + strand, within Psest_1158 at 1259.202 kb on + strand, within Psest_1158 at 1259.202 kb on + strand, within Psest_1158 at 1259.202 kb on + strand, within Psest_1158 at 1259.202 kb on + strand, within Psest_1158 at 1259.202 kb on + strand, within Psest_1158 at 1259.202 kb on + strand, within Psest_1158 at 1259.202 kb on + strand, within Psest_1158 at 1259.202 kb on + strand, within Psest_1158 at 1259.202 kb on + strand, within Psest_1158 at 1259.202 kb on + strand, within Psest_1158 at 1259.202 kb on + strand, within Psest_1158 at 1259.203 kb on - strand, within Psest_1158 at 1259.203 kb on - strand, within Psest_1158 at 1259.203 kb on - strand, within Psest_1158 at 1259.203 kb on - strand, within Psest_1158 at 1259.203 kb on - strand, within Psest_1158 at 1259.203 kb on - strand, within Psest_1158 at 1259.203 kb on - strand, within Psest_1158 at 1259.203 kb on - strand, within Psest_1158 at 1259.203 kb on - strand, within Psest_1158 at 1259.203 kb on - strand, within Psest_1158 at 1259.203 kb on - strand, within Psest_1158 at 1259.204 kb on + strand, within Psest_1158 at 1259.204 kb on + strand, within Psest_1158 at 1259.204 kb on + strand, within Psest_1158 at 1259.204 kb on + strand, within Psest_1158 at 1259.204 kb on + strand, within Psest_1158 at 1259.204 kb on + strand, within Psest_1158 at 1259.204 kb on + strand, within Psest_1158 at 1259.205 kb on - strand, within Psest_1158 at 1259.205 kb on - strand, within Psest_1158 at 1259.538 kb on + strand at 1259.538 kb on + strand at 1259.553 kb on + strand at 1259.575 kb on + strand at 1259.866 kb on + strand, within Psest_1159 at 1259.990 kb on + strand, within Psest_1159 at 1259.991 kb on - strand, within Psest_1159 at 1259.991 kb on - strand, within Psest_1159 at 1260.002 kb on + strand, within Psest_1159 at 1260.002 kb on + strand, within Psest_1159 at 1260.003 kb on - strand, within Psest_1159 at 1260.136 kb on + strand, within Psest_1159 at 1260.137 kb on - strand, within Psest_1159 at 1260.206 kb on + strand, within Psest_1159 at 1260.207 kb on - strand, within Psest_1159 at 1260.284 kb on + strand, within Psest_1159 at 1260.285 kb on - strand, within Psest_1159 at 1260.335 kb on + strand, within Psest_1159 at 1260.335 kb on + strand, within Psest_1159 at 1260.335 kb on + strand, within Psest_1159 at 1260.336 kb on - strand, within Psest_1159 at 1260.511 kb on + strand, within Psest_1159 at 1260.686 kb on + strand, within Psest_1159 at 1260.686 kb on + strand, within Psest_1159 at 1260.686 kb on + strand, within Psest_1159 at 1260.686 kb on + strand, within Psest_1159 at 1260.687 kb on - strand, within Psest_1159 at 1260.687 kb on - strand, within Psest_1159 at 1261.010 kb on - strand at 1261.010 kb on - strand at 1261.010 kb on - strand at 1261.235 kb on + strand at 1261.235 kb on + strand at 1261.236 kb on - strand at 1261.250 kb on + strand, within Psest_1160 at 1261.251 kb on - strand, within Psest_1160 at 1261.251 kb on - strand, within Psest_1160 at 1261.364 kb on + strand, within Psest_1160 at 1261.607 kb on + strand, within Psest_1160 at 1261.607 kb on + strand, within Psest_1160 at 1261.608 kb on - strand, within Psest_1160 at 1261.899 kb on + strand, within Psest_1161 at 1261.900 kb on - strand, within Psest_1161 at 1261.900 kb on - strand, within Psest_1161
Per-strain Table
Position Strand Gene LocusTag Fraction Def Neomycin 0.0008 mg/ml remove 1,258,956 + Psest_1158 0.61 -2.0 1,258,957 - Psest_1158 0.61 -1.0 1,259,013 + Psest_1158 0.65 -1.5 1,259,013 + Psest_1158 0.65 -2.5 1,259,014 - Psest_1158 0.65 -0.9 1,259,014 - Psest_1158 0.65 -1.3 1,259,014 - Psest_1158 0.65 -0.9 1,259,014 - Psest_1158 0.65 -1.1 1,259,014 - Psest_1158 0.65 -0.5 1,259,014 - Psest_1158 0.65 -1.1 1,259,014 - Psest_1158 0.65 -1.9 1,259,014 - Psest_1158 0.65 -0.9 1,259,014 - Psest_1158 0.65 +1.0 1,259,202 + Psest_1158 0.78 -1.2 1,259,202 + Psest_1158 0.78 -2.3 1,259,202 + Psest_1158 0.78 -1.6 1,259,202 + Psest_1158 0.78 -1.1 1,259,202 + Psest_1158 0.78 -0.2 1,259,202 + Psest_1158 0.78 +0.1 1,259,202 + Psest_1158 0.78 -2.2 1,259,202 + Psest_1158 0.78 -1.6 1,259,202 + Psest_1158 0.78 +1.8 1,259,202 + Psest_1158 0.78 -0.3 1,259,202 + Psest_1158 0.78 -1.2 1,259,202 + Psest_1158 0.78 +0.0 1,259,203 - Psest_1158 0.78 -1.4 1,259,203 - Psest_1158 0.78 -0.5 1,259,203 - Psest_1158 0.78 +0.5 1,259,203 - Psest_1158 0.78 -1.5 1,259,203 - Psest_1158 0.78 -3.0 1,259,203 - Psest_1158 0.78 -0.5 1,259,203 - Psest_1158 0.78 +1.0 1,259,203 - Psest_1158 0.78 -1.5 1,259,203 - Psest_1158 0.78 -0.4 1,259,203 - Psest_1158 0.78 -0.4 1,259,203 - Psest_1158 0.78 +0.7 1,259,204 + Psest_1158 0.78 +1.2 1,259,204 + Psest_1158 0.78 +0.1 1,259,204 + Psest_1158 0.78 -3.6 1,259,204 + Psest_1158 0.78 -0.2 1,259,204 + Psest_1158 0.78 +0.5 1,259,204 + Psest_1158 0.78 -1.1 1,259,204 + Psest_1158 0.78 +0.4 1,259,205 - Psest_1158 0.78 -1.8 1,259,205 - Psest_1158 0.78 -2.3 1,259,538 + -0.8 1,259,538 + +0.3 1,259,553 + -2.2 1,259,575 + +0.4 1,259,866 + Psest_1159 0.14 +0.1 1,259,990 + Psest_1159 0.23 -1.9 1,259,991 - Psest_1159 0.23 +0.7 1,259,991 - Psest_1159 0.23 +1.0 1,260,002 + Psest_1159 0.24 -0.2 1,260,002 + Psest_1159 0.24 -2.0 1,260,003 - Psest_1159 0.24 -0.5 1,260,136 + Psest_1159 0.34 -1.3 1,260,137 - Psest_1159 0.34 -1.1 1,260,206 + Psest_1159 0.39 -2.5 1,260,207 - Psest_1159 0.39 -0.4 1,260,284 + Psest_1159 0.45 +0.1 1,260,285 - Psest_1159 0.45 -0.9 1,260,335 + Psest_1159 0.49 -1.9 1,260,335 + Psest_1159 0.49 -1.5 1,260,335 + Psest_1159 0.49 -1.0 1,260,336 - Psest_1159 0.49 -1.3 1,260,511 + Psest_1159 0.62 +0.4 1,260,686 + Psest_1159 0.75 -2.5 1,260,686 + Psest_1159 0.75 -2.1 1,260,686 + Psest_1159 0.75 -0.7 1,260,686 + Psest_1159 0.75 -2.5 1,260,687 - Psest_1159 0.75 -2.3 1,260,687 - Psest_1159 0.75 -1.1 1,261,010 - -1.3 1,261,010 - -1.8 1,261,010 - +0.0 1,261,235 + +0.3 1,261,235 + +1.5 1,261,236 - +0.6 1,261,250 + Psest_1160 0.12 -0.1 1,261,251 - Psest_1160 0.12 -0.6 1,261,251 - Psest_1160 0.12 -0.9 1,261,364 + Psest_1160 0.31 -0.3 1,261,607 + Psest_1160 0.74 -0.6 1,261,607 + Psest_1160 0.74 +0.4 1,261,608 - Psest_1160 0.74 +1.6 1,261,899 + Psest_1161 0.16 -0.8 1,261,900 - Psest_1161 0.16 +1.7 1,261,900 - Psest_1161 0.16 -1.7
Or see this region's nucleotide sequence