Experiment: Def Neomycin 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0852 and Psest_0853 overlap by 14 nucleotides Psest_0853 and Psest_0854 are separated by 18 nucleotides Psest_0854 and Psest_0855 are separated by 70 nucleotides
Psest_0852: Psest_0852 - glucose-6-phosphate 1-dehydrogenase, at 922,531 to 924,000
_0852
Psest_0853: Psest_0853 - 6-phosphogluconolactonase, at 923,987 to 924,700
_0853
Psest_0854: Psest_0854 - Entner-Doudoroff aldolase, at 924,719 to 925,456
_0854
Psest_0855: Psest_0855 - NAD-dependent aldehyde dehydrogenases, at 925,527 to 927,155
_0855
Position (kb) 923
924
925 Strain fitness (log2 ratio) -2
-1
0
1
2 at 923.041 kb on + strand, within Psest_0852 at 923.041 kb on + strand, within Psest_0852 at 923.056 kb on + strand, within Psest_0852 at 923.387 kb on - strand, within Psest_0852 at 923.426 kb on - strand, within Psest_0852 at 923.524 kb on + strand, within Psest_0852 at 923.524 kb on + strand, within Psest_0852 at 923.524 kb on + strand, within Psest_0852 at 923.596 kb on + strand, within Psest_0852 at 923.596 kb on + strand, within Psest_0852 at 923.597 kb on - strand, within Psest_0852 at 923.758 kb on + strand, within Psest_0852 at 923.759 kb on - strand, within Psest_0852 at 923.855 kb on - strand at 923.920 kb on + strand at 923.986 kb on + strand at 923.986 kb on + strand at 923.998 kb on + strand at 924.321 kb on - strand, within Psest_0853 at 924.370 kb on - strand, within Psest_0853 at 924.396 kb on + strand, within Psest_0853 at 924.396 kb on + strand, within Psest_0853 at 924.396 kb on + strand, within Psest_0853 at 924.396 kb on + strand, within Psest_0853 at 924.396 kb on + strand, within Psest_0853 at 924.397 kb on - strand, within Psest_0853 at 924.397 kb on - strand, within Psest_0853 at 924.397 kb on - strand, within Psest_0853 at 924.397 kb on - strand, within Psest_0853 at 924.397 kb on - strand, within Psest_0853 at 924.686 kb on + strand at 924.687 kb on - strand at 924.687 kb on - strand at 924.727 kb on + strand at 924.728 kb on - strand at 925.262 kb on + strand, within Psest_0854 at 925.263 kb on - strand, within Psest_0854 at 925.620 kb on + strand at 925.621 kb on - strand at 925.621 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Neomycin 0.0002 mg/ml remove 923,041 + Psest_0852 0.35 -0.5 923,041 + Psest_0852 0.35 +0.7 923,056 + Psest_0852 0.36 +0.2 923,387 - Psest_0852 0.58 -0.1 923,426 - Psest_0852 0.61 -2.2 923,524 + Psest_0852 0.68 -1.3 923,524 + Psest_0852 0.68 -0.0 923,524 + Psest_0852 0.68 +0.0 923,596 + Psest_0852 0.72 +2.7 923,596 + Psest_0852 0.72 +0.2 923,597 - Psest_0852 0.73 +0.1 923,758 + Psest_0852 0.83 +0.8 923,759 - Psest_0852 0.84 +0.8 923,855 - +1.7 923,920 + -0.2 923,986 + -0.7 923,986 + +0.1 923,998 + +0.1 924,321 - Psest_0853 0.47 -0.9 924,370 - Psest_0853 0.54 +1.2 924,396 + Psest_0853 0.57 +0.1 924,396 + Psest_0853 0.57 +0.1 924,396 + Psest_0853 0.57 -0.2 924,396 + Psest_0853 0.57 +1.1 924,396 + Psest_0853 0.57 -1.2 924,397 - Psest_0853 0.57 +1.1 924,397 - Psest_0853 0.57 -0.2 924,397 - Psest_0853 0.57 +0.7 924,397 - Psest_0853 0.57 -1.7 924,397 - Psest_0853 0.57 -1.7 924,686 + +0.7 924,687 - -0.2 924,687 - +0.1 924,727 + +1.7 924,728 - +0.7 925,262 + Psest_0854 0.74 +0.9 925,263 - Psest_0854 0.74 -0.3 925,620 + -0.3 925,621 - +1.7 925,621 - +1.7
Or see this region's nucleotide sequence