Experiment: Def Neomycin 0.0002 mg/ml 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
    
        
         
    
        
         
    
        
         
    
         
     
500 nt Psest_3350 and Psest_3351 are separated by 406 nucleotides Psest_3351 and Psest_3352 are separated by 59 nucleotides Psest_3352 and Psest_3353 are separated by 79 nucleotides 
        Psest_3350: Psest_3350 - Diaminopimelate decarboxylase, at 3,518,066 to 3,519,229 
        _3350 
         
        
        Psest_3351: Psest_3351 - peptide chain release factor 3, at 3,519,636 to 3,521,219 
        _3351 
         
        
        Psest_3352: Psest_3352 - DnaK suppressor protein, at 3,521,279 to 3,521,605 
        _3352 
         
        
        Psest_3353: Psest_3353 - PTS system, fructose-specific, IIB component/PTS system, fructose subfamily, IIC component, at 3,521,685 to 3,523,424 
        _3353 
         Position (kb) 3519 
3520 
3521 
3522 Strain fitness (log2 ratio) -2 
-1 
0 
1 
2 at 3518.811 kb on + strand, within Psest_3350 at 3518.811 kb on + strand, within Psest_3350 at 3518.812 kb on - strand, within Psest_3350 at 3518.812 kb on - strand, within Psest_3350 at 3518.812 kb on - strand, within Psest_3350 at 3518.988 kb on + strand, within Psest_3350 at 3518.989 kb on - strand, within Psest_3350 at 3518.989 kb on - strand, within Psest_3350 at 3518.989 kb on - strand, within Psest_3350 at 3519.069 kb on + strand, within Psest_3350 at 3519.069 kb on + strand, within Psest_3350 at 3519.069 kb on + strand, within Psest_3350 at 3519.070 kb on - strand, within Psest_3350 at 3519.070 kb on - strand, within Psest_3350 at 3519.072 kb on + strand, within Psest_3350 at 3519.072 kb on + strand, within Psest_3350 at 3519.072 kb on + strand, within Psest_3350 at 3519.072 kb on + strand, within Psest_3350 at 3519.111 kb on + strand, within Psest_3350 at 3519.207 kb on + strand at 3519.207 kb on + strand at 3519.207 kb on + strand at 3519.207 kb on + strand at 3519.207 kb on + strand at 3519.207 kb on + strand at 3519.207 kb on + strand at 3519.207 kb on + strand at 3519.208 kb on - strand at 3519.329 kb on + strand at 3519.330 kb on - strand at 3519.330 kb on - strand at 3519.380 kb on - strand at 3519.429 kb on + strand at 3519.446 kb on + strand at 3519.446 kb on + strand at 3519.446 kb on + strand at 3519.446 kb on + strand at 3519.447 kb on - strand at 3519.447 kb on - strand at 3519.447 kb on - strand at 3519.447 kb on - strand at 3519.447 kb on - strand at 3519.447 kb on - strand at 3519.609 kb on + strand at 3519.927 kb on + strand, within Psest_3351 at 3519.927 kb on + strand, within Psest_3351 at 3519.928 kb on - strand, within Psest_3351 at 3520.049 kb on + strand, within Psest_3351 at 3520.177 kb on - strand, within Psest_3351 at 3520.177 kb on - strand, within Psest_3351 at 3520.194 kb on + strand, within Psest_3351 at 3520.194 kb on + strand, within Psest_3351 at 3520.194 kb on + strand, within Psest_3351 at 3520.195 kb on - strand, within Psest_3351 at 3520.195 kb on - strand, within Psest_3351 at 3520.206 kb on + strand, within Psest_3351 at 3520.296 kb on + strand, within Psest_3351 at 3520.583 kb on + strand, within Psest_3351 at 3520.583 kb on + strand, within Psest_3351 at 3520.584 kb on - strand, within Psest_3351 at 3520.584 kb on - strand, within Psest_3351 at 3520.584 kb on - strand, within Psest_3351 at 3520.584 kb on - strand, within Psest_3351 at 3520.600 kb on - strand, within Psest_3351 at 3520.704 kb on + strand, within Psest_3351 at 3520.704 kb on + strand, within Psest_3351 at 3520.705 kb on - strand, within Psest_3351 at 3520.995 kb on + strand, within Psest_3351 at 3520.995 kb on + strand, within Psest_3351 at 3520.995 kb on + strand, within Psest_3351 at 3520.995 kb on + strand, within Psest_3351 at 3520.995 kb on + strand, within Psest_3351 at 3520.995 kb on + strand, within Psest_3351 at 3520.995 kb on + strand, within Psest_3351 at 3520.995 kb on + strand, within Psest_3351 at 3520.995 kb on + strand, within Psest_3351 at 3520.996 kb on - strand, within Psest_3351 at 3520.996 kb on - strand, within Psest_3351 at 3520.996 kb on - strand, within Psest_3351 at 3520.996 kb on - strand, within Psest_3351 at 3521.133 kb on + strand at 3521.133 kb on + strand at 3521.226 kb on - strand at 3521.226 kb on - strand at 3521.382 kb on + strand, within Psest_3352 at 3521.382 kb on + strand, within Psest_3352 at 3521.382 kb on + strand, within Psest_3352 at 3521.382 kb on + strand, within Psest_3352 at 3521.382 kb on + strand, within Psest_3352 at 3521.382 kb on + strand, within Psest_3352 at 3521.382 kb on + strand, within Psest_3352 at 3521.383 kb on - strand, within Psest_3352 at 3521.383 kb on - strand, within Psest_3352 at 3521.383 kb on - strand, within Psest_3352 at 3521.383 kb on - strand, within Psest_3352 at 3521.383 kb on - strand, within Psest_3352 at 3521.383 kb on - strand, within Psest_3352 at 3521.462 kb on + strand, within Psest_3352 at 3521.462 kb on + strand, within Psest_3352 at 3521.462 kb on + strand, within Psest_3352 at 3521.462 kb on + strand, within Psest_3352 at 3521.462 kb on + strand, within Psest_3352 at 3521.463 kb on - strand, within Psest_3352 at 3521.463 kb on - strand, within Psest_3352 at 3521.463 kb on - strand, within Psest_3352 at 3521.556 kb on + strand, within Psest_3352 at 3521.557 kb on - strand, within Psest_3352 at 3521.658 kb on + strand at 3521.658 kb on + strand at 3521.658 kb on + strand at 3521.659 kb on - strand at 3521.659 kb on - strand at 3521.682 kb on + strand at 3521.682 kb on + strand at 3521.740 kb on + strand at 3521.740 kb on + strand at 3521.740 kb on + strand at 3521.740 kb on + strand at 3521.740 kb on + strand at 3521.740 kb on + strand at 3521.740 kb on + strand at 3521.741 kb on - strand at 3521.741 kb on - strand at 3521.741 kb on - strand at 3521.741 kb on - strand at 3521.741 kb on - strand at 3521.741 kb on - strand at 3521.785 kb on + strand at 3521.786 kb on - strand at 3521.786 kb on - strand at 3522.103 kb on + strand, within Psest_3353 at 3522.103 kb on + strand, within Psest_3353 at 3522.139 kb on + strand, within Psest_3353 at 3522.139 kb on + strand, within Psest_3353 at 3522.196 kb on - strand, within Psest_3353  
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction Def Neomycin 0.0002 mg/ml remove 3,518,811 +  Psest_3350 0.64  +0.4 3,518,811 +  Psest_3350 0.64  +0.0 3,518,812 -  Psest_3350 0.64  -0.2 3,518,812 -  Psest_3350 0.64  +0.5 3,518,812 -  Psest_3350 0.64  +0.5 3,518,988 +  Psest_3350 0.79  +0.1 3,518,989 -  Psest_3350 0.79  -0.3 3,518,989 -  Psest_3350 0.79  -1.0 3,518,989 -  Psest_3350 0.79  -1.2 3,519,069 +  Psest_3350 0.86  +0.0 3,519,069 +  Psest_3350 0.86  -2.0 3,519,069 +  Psest_3350 0.86  -0.4 3,519,070 -  Psest_3350 0.86  -0.5 3,519,070 -  Psest_3350 0.86  -1.8 3,519,072 +  Psest_3350 0.86  +0.1 3,519,072 +  Psest_3350 0.86  -0.4 3,519,072 +  Psest_3350 0.86  +1.4 3,519,072 +  Psest_3350 0.86  -0.3 3,519,111 +  Psest_3350 0.90  -0.7 3,519,207 +  -0.5 3,519,207 +  -2.3 3,519,207 +  -0.6 3,519,207 +  +0.0 3,519,207 +  -0.6 3,519,207 +  +0.6 3,519,207 +  -0.4 3,519,207 +  -1.0 3,519,208 -  -1.6 3,519,329 +  -1.0 3,519,330 -  +1.0 3,519,330 -  -0.0 3,519,380 -  -0.4 3,519,429 +  -0.6 3,519,446 +  +0.0 3,519,446 +  -0.2 3,519,446 +  +1.2 3,519,446 +  -0.7 3,519,447 -  -2.0 3,519,447 -  -0.2 3,519,447 -  -1.0 3,519,447 -  -1.4 3,519,447 -  +0.0 3,519,447 -  -1.1 3,519,609 +  +0.0 3,519,927 +  Psest_3351 0.18  +0.3 3,519,927 +  Psest_3351 0.18  +0.0 3,519,928 -  Psest_3351 0.18  -1.0 3,520,049 +  Psest_3351 0.26  -0.1 3,520,177 -  Psest_3351 0.34  -1.2 3,520,177 -  Psest_3351 0.34  -0.7 3,520,194 +  Psest_3351 0.35  -0.8 3,520,194 +  Psest_3351 0.35  -0.4 3,520,194 +  Psest_3351 0.35  +0.6 3,520,195 -  Psest_3351 0.35  -0.3 3,520,195 -  Psest_3351 0.35  -1.3 3,520,206 +  Psest_3351 0.36  +0.6 3,520,296 +  Psest_3351 0.42  +0.0 3,520,583 +  Psest_3351 0.60  -1.4 3,520,583 +  Psest_3351 0.60  -1.2 3,520,584 -  Psest_3351 0.60  -1.2 3,520,584 -  Psest_3351 0.60  +1.6 3,520,584 -  Psest_3351 0.60  +0.6 3,520,584 -  Psest_3351 0.60  -1.2 3,520,600 -  Psest_3351 0.61  +0.0 3,520,704 +  Psest_3351 0.67  -0.1 3,520,704 +  Psest_3351 0.67  -1.7 3,520,705 -  Psest_3351 0.67  +0.6 3,520,995 +  Psest_3351 0.86  +1.6 3,520,995 +  Psest_3351 0.86  +2.6 3,520,995 +  Psest_3351 0.86  -0.3 3,520,995 +  Psest_3351 0.86  -0.4 3,520,995 +  Psest_3351 0.86  -0.4 3,520,995 +  Psest_3351 0.86  -1.0 3,520,995 +  Psest_3351 0.86  -0.7 3,520,995 +  Psest_3351 0.86  -1.6 3,520,995 +  Psest_3351 0.86  +0.3 3,520,996 -  Psest_3351 0.86  +0.6 3,520,996 -  Psest_3351 0.86  -1.2 3,520,996 -  Psest_3351 0.86  -0.4 3,520,996 -  Psest_3351 0.86  -1.0 3,521,133 +  -1.4 3,521,133 +  -1.4 3,521,226 -  +0.6 3,521,226 -  -0.0 3,521,382 +  Psest_3352 0.31  +0.2 3,521,382 +  Psest_3352 0.31  +0.9 3,521,382 +  Psest_3352 0.31  +0.4 3,521,382 +  Psest_3352 0.31  -0.2 3,521,382 +  Psest_3352 0.31  +0.1 3,521,382 +  Psest_3352 0.31  +1.6 3,521,382 +  Psest_3352 0.31  -1.9 3,521,383 -  Psest_3352 0.32  -0.1 3,521,383 -  Psest_3352 0.32  -0.3 3,521,383 -  Psest_3352 0.32  +0.4 3,521,383 -  Psest_3352 0.32  -1.1 3,521,383 -  Psest_3352 0.32  +0.6 3,521,383 -  Psest_3352 0.32  -0.7 3,521,462 +  Psest_3352 0.56  -1.0 3,521,462 +  Psest_3352 0.56  -0.8 3,521,462 +  Psest_3352 0.56  +0.1 3,521,462 +  Psest_3352 0.56  -1.0 3,521,462 +  Psest_3352 0.56  -0.1 3,521,463 -  Psest_3352 0.56  -0.6 3,521,463 -  Psest_3352 0.56  -0.2 3,521,463 -  Psest_3352 0.56  +0.1 3,521,556 +  Psest_3352 0.85  +0.3 3,521,557 -  Psest_3352 0.85  -0.1 3,521,658 +  -0.4 3,521,658 +  -0.6 3,521,658 +  -0.5 3,521,659 -  -0.4 3,521,659 -  +0.6 3,521,682 +  +0.0 3,521,682 +  -1.4 3,521,740 +  -0.1 3,521,740 +  -1.0 3,521,740 +  +0.1 3,521,740 +  -0.2 3,521,740 +  +1.0 3,521,740 +  -1.0 3,521,740 +  -0.4 3,521,741 -  -0.4 3,521,741 -  -0.6 3,521,741 -  +1.9 3,521,741 -  +0.4 3,521,741 -  +1.1 3,521,741 -  +0.4 3,521,785 +  -0.7 3,521,786 -  -0.3 3,521,786 -  +0.6 3,522,103 +  Psest_3353 0.24  +0.2 3,522,103 +  Psest_3353 0.24  +0.3 3,522,139 +  Psest_3353 0.26  +0.3 3,522,139 +  Psest_3353 0.26  -0.1 3,522,196 -  Psest_3353 0.29  -0.4 
Or see this region's nucleotide sequence