Experiment: Def Neomycin 0.0002 mg/ml 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
     
    
        
         
     
    
        
         
     
    
        
         
     
    
         
     
     
 
500 nt Psest_1177 and Psest_1178 are separated by 32 nucleotides Psest_1178 and Psest_1179 are separated by 328 nucleotides  
        
        Psest_1177: Psest_1177 - pyruvate kinase, at 1,278,310 to 1,279,725 
         
        _1177 
         
        
        Psest_1178: Psest_1178 - D-glycerate 2-kinase (EC 2.7.1.-) (from data) , at 1,279,758 to 1,281,029 
         
        _1178 
         
        
        Psest_1179: Psest_1179 - 2-hydroxy-3-oxopropionate reductase, at 1,281,358 to 1,282,248 
         
        _1179 
         Position (kb)  
1279 
 
1280 
 
1281 
 
1282 Strain fitness (log2 ratio)  
-2 
 
-1 
 
0 
 
1 at 1279.699 kb on + strand at 1279.699 kb on + strand at 1279.699 kb on + strand at 1279.699 kb on + strand at 1279.699 kb on + strand at 1279.699 kb on + strand at 1279.699 kb on + strand at 1279.699 kb on + strand at 1279.700 kb on - strand at 1279.700 kb on - strand at 1279.700 kb on - strand at 1279.700 kb on - strand at 1279.700 kb on - strand at 1279.700 kb on - strand at 1279.759 kb on + strand at 1279.852 kb on + strand at 1279.853 kb on - strand at 1279.853 kb on - strand at 1279.918 kb on + strand, within Psest_1178 at 1279.919 kb on - strand, within Psest_1178 at 1279.919 kb on - strand, within Psest_1178 at 1279.993 kb on + strand, within Psest_1178 at 1279.993 kb on + strand, within Psest_1178 at 1280.488 kb on + strand, within Psest_1178 at 1280.488 kb on + strand, within Psest_1178 at 1280.488 kb on + strand, within Psest_1178 at 1280.488 kb on + strand, within Psest_1178 at 1280.489 kb on - strand, within Psest_1178 at 1280.489 kb on - strand, within Psest_1178 at 1280.489 kb on - strand, within Psest_1178 at 1280.489 kb on - strand, within Psest_1178 at 1280.489 kb on - strand, within Psest_1178 at 1280.489 kb on - strand, within Psest_1178 at 1280.494 kb on + strand, within Psest_1178 at 1280.494 kb on + strand, within Psest_1178 at 1280.495 kb on - strand, within Psest_1178 at 1280.495 kb on - strand, within Psest_1178 at 1280.680 kb on - strand, within Psest_1178 at 1281.043 kb on + strand at 1281.043 kb on + strand at 1281.043 kb on + strand at 1281.043 kb on + strand at 1281.043 kb on + strand at 1281.043 kb on + strand at 1281.043 kb on + strand at 1281.044 kb on - strand at 1281.044 kb on - strand at 1281.044 kb on - strand at 1281.044 kb on - strand at 1281.044 kb on - strand at 1281.251 kb on - strand at 1281.360 kb on - strand at 1281.902 kb on + strand, within Psest_1179 at 1281.902 kb on + strand, within Psest_1179  
 
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction   Def Neomycin 0.0002 mg/ml             remove   1,279,699   +        +1.1   1,279,699   +        +0.2   1,279,699   +        -0.1   1,279,699   +        +0.1   1,279,699   +        -0.9   1,279,699   +        -1.3   1,279,699   +        -0.3   1,279,699   +        +1.6   1,279,700   -        +0.4   1,279,700   -        +0.6   1,279,700   -        -0.4   1,279,700   -        +0.2   1,279,700   -        +0.0   1,279,700   -        +1.1   1,279,759   +        +1.6   1,279,852   +        -0.1   1,279,853   -        +0.4   1,279,853   -        -0.1   1,279,918   +    Psest_1178   0.13  -0.7   1,279,919   -    Psest_1178   0.13  +1.5   1,279,919   -    Psest_1178   0.13  +0.6   1,279,993   +    Psest_1178   0.18  +0.3   1,279,993   +    Psest_1178   0.18  +0.0   1,280,488   +    Psest_1178   0.57  +0.9   1,280,488   +    Psest_1178   0.57  -0.7   1,280,488   +    Psest_1178   0.57  +0.8   1,280,488   +    Psest_1178   0.57  -2.1   1,280,489   -    Psest_1178   0.57  +0.9   1,280,489   -    Psest_1178   0.57  +0.8   1,280,489   -    Psest_1178   0.57  +0.0   1,280,489   -    Psest_1178   0.57  +0.5   1,280,489   -    Psest_1178   0.57  +0.8   1,280,489   -    Psest_1178   0.57  +1.0   1,280,494   +    Psest_1178   0.58  -0.5   1,280,494   +    Psest_1178   0.58  -1.0   1,280,495   -    Psest_1178   0.58  +0.1   1,280,495   -    Psest_1178   0.58  +1.0   1,280,680   -    Psest_1178   0.72  +1.2   1,281,043   +        +0.5   1,281,043   +        +0.8   1,281,043   +        +1.3   1,281,043   +        +0.5   1,281,043   +        +0.5   1,281,043   +        -0.9   1,281,043   +        +0.2   1,281,044   -        +0.6   1,281,044   -        -1.1   1,281,044   -        +0.2   1,281,044   -        +0.3   1,281,044   -        +0.1   1,281,251   -        -0.6   1,281,360   -        +0.4   1,281,902   +    Psest_1179   0.61  -0.2   1,281,902   +    Psest_1179   0.61  +1.2 
 
Or see this region's nucleotide sequence