Experiment: Def Lomefloxacin 0.00004 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0957 and Psest_0958 are separated by 210 nucleotides Psest_0958 and Psest_0959 overlap by 8 nucleotides Psest_0959 and Psest_0960 are separated by 40 nucleotides Psest_0960 and Psest_0961 are separated by 95 nucleotides Psest_0961 and Psest_0962 are separated by 58 nucleotides
Psest_0957: Psest_0957 - SsrA-binding protein, at 1,039,260 to 1,039,742
_0957
Psest_0958: Psest_0958 - Oligoketide cyclase/lipid transport protein, at 1,039,953 to 1,040,387
_0958
Psest_0959: Psest_0959 - Uncharacterized protein conserved in bacteria, at 1,040,380 to 1,040,694
_0959
Psest_0960: Psest_0960 - Small protein A (tmRNA-binding), at 1,040,735 to 1,041,250
_0960
Psest_0961: Psest_0961 - Fe2+/Zn2+ uptake regulation proteins, at 1,041,346 to 1,041,750
_0961
Psest_0962: Psest_0962 - DNA repair protein RecN, at 1,041,809 to 1,043,482
_0962
Position (kb)
1040
1041
1042 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1039.755 kb on - strand at 1039.757 kb on - strand at 1039.757 kb on - strand at 1039.757 kb on - strand at 1039.941 kb on + strand at 1039.941 kb on + strand at 1039.941 kb on + strand at 1039.941 kb on + strand at 1039.941 kb on + strand at 1039.941 kb on + strand at 1039.941 kb on + strand at 1039.941 kb on + strand at 1039.942 kb on - strand at 1039.942 kb on - strand at 1039.942 kb on - strand at 1039.942 kb on - strand at 1040.026 kb on + strand, within Psest_0958 at 1040.026 kb on + strand, within Psest_0958 at 1040.027 kb on - strand, within Psest_0958 at 1040.027 kb on - strand, within Psest_0958 at 1040.081 kb on + strand, within Psest_0958 at 1040.082 kb on - strand, within Psest_0958 at 1040.205 kb on - strand, within Psest_0958 at 1040.212 kb on + strand, within Psest_0958 at 1040.212 kb on + strand, within Psest_0958 at 1040.212 kb on + strand, within Psest_0958 at 1040.212 kb on + strand, within Psest_0958 at 1040.212 kb on + strand, within Psest_0958 at 1040.212 kb on + strand, within Psest_0958 at 1040.212 kb on + strand, within Psest_0958 at 1040.212 kb on + strand, within Psest_0958 at 1040.212 kb on + strand, within Psest_0958 at 1040.212 kb on + strand, within Psest_0958 at 1040.212 kb on + strand, within Psest_0958 at 1040.212 kb on + strand, within Psest_0958 at 1040.212 kb on + strand, within Psest_0958 at 1040.212 kb on + strand, within Psest_0958 at 1040.212 kb on + strand, within Psest_0958 at 1040.212 kb on + strand, within Psest_0958 at 1040.212 kb on + strand, within Psest_0958 at 1040.213 kb on - strand, within Psest_0958 at 1040.213 kb on - strand, within Psest_0958 at 1040.213 kb on - strand, within Psest_0958 at 1040.213 kb on - strand, within Psest_0958 at 1040.213 kb on - strand, within Psest_0958 at 1040.213 kb on - strand, within Psest_0958 at 1040.213 kb on - strand, within Psest_0958 at 1040.213 kb on - strand, within Psest_0958 at 1040.213 kb on - strand, within Psest_0958 at 1040.213 kb on - strand, within Psest_0958 at 1040.213 kb on - strand, within Psest_0958 at 1040.213 kb on - strand, within Psest_0958 at 1040.213 kb on - strand, within Psest_0958 at 1040.379 kb on + strand at 1040.379 kb on + strand at 1040.379 kb on + strand at 1040.380 kb on - strand at 1040.413 kb on + strand, within Psest_0959 at 1040.413 kb on + strand, within Psest_0959 at 1040.467 kb on - strand, within Psest_0959 at 1040.561 kb on + strand, within Psest_0959 at 1040.611 kb on + strand, within Psest_0959 at 1040.612 kb on - strand, within Psest_0959 at 1042.004 kb on + strand, within Psest_0962 at 1042.022 kb on + strand, within Psest_0962 at 1042.022 kb on + strand, within Psest_0962 at 1042.023 kb on - strand, within Psest_0962 at 1042.023 kb on - strand, within Psest_0962 at 1042.023 kb on - strand, within Psest_0962 at 1042.109 kb on + strand, within Psest_0962 at 1042.110 kb on - strand, within Psest_0962 at 1042.110 kb on - strand, within Psest_0962 at 1042.173 kb on - strand, within Psest_0962 at 1042.173 kb on - strand, within Psest_0962
Per-strain Table
Position Strand Gene LocusTag Fraction Def Lomefloxacin 0.00004 mM remove 1,039,755 - -1.9 1,039,757 - +0.2 1,039,757 - -0.5 1,039,757 - -0.3 1,039,941 + +1.3 1,039,941 + -1.1 1,039,941 + +0.5 1,039,941 + -0.6 1,039,941 + +0.4 1,039,941 + -0.9 1,039,941 + +0.5 1,039,941 + -0.2 1,039,942 - -0.3 1,039,942 - -0.9 1,039,942 - +0.5 1,039,942 - +0.1 1,040,026 + Psest_0958 0.17 -0.9 1,040,026 + Psest_0958 0.17 -0.6 1,040,027 - Psest_0958 0.17 -0.9 1,040,027 - Psest_0958 0.17 +0.6 1,040,081 + Psest_0958 0.29 -0.2 1,040,082 - Psest_0958 0.30 -0.5 1,040,205 - Psest_0958 0.58 -1.6 1,040,212 + Psest_0958 0.60 -2.0 1,040,212 + Psest_0958 0.60 +0.1 1,040,212 + Psest_0958 0.60 -0.2 1,040,212 + Psest_0958 0.60 +0.5 1,040,212 + Psest_0958 0.60 +0.0 1,040,212 + Psest_0958 0.60 -0.4 1,040,212 + Psest_0958 0.60 +0.6 1,040,212 + Psest_0958 0.60 +0.5 1,040,212 + Psest_0958 0.60 +0.7 1,040,212 + Psest_0958 0.60 -0.7 1,040,212 + Psest_0958 0.60 -0.1 1,040,212 + Psest_0958 0.60 +0.1 1,040,212 + Psest_0958 0.60 +0.4 1,040,212 + Psest_0958 0.60 -0.2 1,040,212 + Psest_0958 0.60 -0.5 1,040,212 + Psest_0958 0.60 -0.5 1,040,212 + Psest_0958 0.60 +1.1 1,040,213 - Psest_0958 0.60 -1.5 1,040,213 - Psest_0958 0.60 -3.7 1,040,213 - Psest_0958 0.60 -1.4 1,040,213 - Psest_0958 0.60 -0.8 1,040,213 - Psest_0958 0.60 -0.8 1,040,213 - Psest_0958 0.60 -0.9 1,040,213 - Psest_0958 0.60 -1.0 1,040,213 - Psest_0958 0.60 -0.9 1,040,213 - Psest_0958 0.60 -0.8 1,040,213 - Psest_0958 0.60 -1.4 1,040,213 - Psest_0958 0.60 -0.8 1,040,213 - Psest_0958 0.60 -1.7 1,040,213 - Psest_0958 0.60 -1.7 1,040,379 + -0.2 1,040,379 + -0.3 1,040,379 + -1.1 1,040,380 - +0.2 1,040,413 + Psest_0959 0.10 -1.9 1,040,413 + Psest_0959 0.10 +0.1 1,040,467 - Psest_0959 0.28 +1.0 1,040,561 + Psest_0959 0.57 +0.6 1,040,611 + Psest_0959 0.73 +1.0 1,040,612 - Psest_0959 0.74 -1.5 1,042,004 + Psest_0962 0.12 -2.2 1,042,022 + Psest_0962 0.13 -1.2 1,042,022 + Psest_0962 0.13 +0.6 1,042,023 - Psest_0962 0.13 -0.5 1,042,023 - Psest_0962 0.13 -0.2 1,042,023 - Psest_0962 0.13 -1.5 1,042,109 + Psest_0962 0.18 -0.7 1,042,110 - Psest_0962 0.18 -0.2 1,042,110 - Psest_0962 0.18 -0.9 1,042,173 - Psest_0962 0.22 -2.2 1,042,173 - Psest_0962 0.22 -1.6
Or see this region's nucleotide sequence