Experiment: Def Lomefloxacin 0.00004 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1495 and Psest_1496 are separated by 73 nucleotides Psest_1496 and Psest_1497 are separated by 3 nucleotides Psest_1497 and Psest_1498 are separated by 51 nucleotides Psest_1498 and Psest_1499 are separated by 2 nucleotides
Psest_1495: Psest_1495 - crossover junction endodeoxyribonuclease RuvC, at 1,597,559 to 1,598,083
_1495
Psest_1496: Psest_1496 - Holliday junction DNA helicase, RuvA subunit, at 1,598,157 to 1,598,762
_1496
Psest_1497: Psest_1497 - Holliday junction DNA helicase, RuvB subunit, at 1,598,766 to 1,599,818
_1497
Psest_1498: Psest_1498 - tol-pal system-associated acyl-CoA thioesterase, at 1,599,870 to 1,600,313
_1498
Psest_1499: Psest_1499 - TolQ protein, at 1,600,316 to 1,601,008
_1499
Position (kb)
1598
1599
1600 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1598.113 kb on - strand at 1598.332 kb on + strand, within Psest_1496 at 1598.333 kb on - strand, within Psest_1496 at 1598.333 kb on - strand, within Psest_1496 at 1598.760 kb on + strand at 1598.760 kb on + strand at 1598.760 kb on + strand at 1598.760 kb on + strand at 1598.761 kb on - strand at 1599.446 kb on - strand, within Psest_1497 at 1599.811 kb on + strand at 1599.866 kb on + strand at 1599.869 kb on + strand at 1599.869 kb on + strand at 1599.869 kb on + strand at 1599.921 kb on + strand, within Psest_1498 at 1599.951 kb on + strand, within Psest_1498 at 1599.954 kb on + strand, within Psest_1498 at 1599.963 kb on + strand, within Psest_1498 at 1600.180 kb on + strand, within Psest_1498
Per-strain Table
Position Strand Gene LocusTag Fraction Def Lomefloxacin 0.00004 mM remove 1,598,113 - -1.0 1,598,332 + Psest_1496 0.29 -0.6 1,598,333 - Psest_1496 0.29 -3.5 1,598,333 - Psest_1496 0.29 -2.2 1,598,760 + -0.0 1,598,760 + -0.0 1,598,760 + +0.0 1,598,760 + -1.2 1,598,761 - +0.1 1,599,446 - Psest_1497 0.65 -2.0 1,599,811 + +0.3 1,599,866 + +0.1 1,599,869 + +0.4 1,599,869 + -0.7 1,599,869 + +0.1 1,599,921 + Psest_1498 0.11 +0.6 1,599,951 + Psest_1498 0.18 +0.4 1,599,954 + Psest_1498 0.19 +0.3 1,599,963 + Psest_1498 0.21 +0.6 1,600,180 + Psest_1498 0.70 +0.1
Or see this region's nucleotide sequence