Experiment: Def A22 0.015 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0837 and Psest_0838 are separated by 180 nucleotides Psest_0838 and Psest_0839 are separated by 96 nucleotides Psest_0839 and Psest_0840 are separated by 231 nucleotides
Psest_0837: Psest_0837 - Putative lipid carrier protein, at 908,171 to 908,665
_0837
Psest_0838: Psest_0838 - Uncharacterized conserved protein, at 908,846 to 909,232
_0838
Psest_0839: Psest_0839 - cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases, at 909,329 to 910,006
_0839
Psest_0840: Psest_0840 - anaerobic ribonucleoside-triphosphate reductase, at 910,238 to 912,256
_0840
Position (kb) 909
910
911 Strain fitness (log2 ratio) -2
-1
0
1
2 at 908.764 kb on + strand at 908.764 kb on + strand at 908.764 kb on + strand at 908.764 kb on + strand at 908.764 kb on + strand at 908.764 kb on + strand at 908.764 kb on + strand at 908.764 kb on + strand at 908.765 kb on - strand at 908.765 kb on - strand at 908.765 kb on - strand at 908.765 kb on - strand at 908.765 kb on - strand at 908.765 kb on - strand at 908.765 kb on - strand at 908.765 kb on - strand at 908.765 kb on - strand at 908.765 kb on - strand at 908.842 kb on + strand at 908.842 kb on + strand at 908.842 kb on + strand at 908.842 kb on + strand at 908.842 kb on + strand at 908.842 kb on + strand at 908.843 kb on - strand at 908.843 kb on - strand at 908.843 kb on - strand at 908.843 kb on - strand at 908.843 kb on - strand at 908.843 kb on - strand at 908.843 kb on - strand at 909.202 kb on - strand at 909.721 kb on - strand, within Psest_0839 at 909.846 kb on + strand, within Psest_0839 at 909.846 kb on + strand, within Psest_0839 at 909.847 kb on - strand, within Psest_0839 at 909.847 kb on - strand, within Psest_0839 at 909.847 kb on - strand, within Psest_0839 at 909.847 kb on - strand, within Psest_0839 at 909.847 kb on - strand, within Psest_0839 at 909.847 kb on - strand, within Psest_0839 at 909.919 kb on + strand, within Psest_0839 at 909.919 kb on + strand, within Psest_0839 at 909.919 kb on + strand, within Psest_0839 at 909.919 kb on + strand, within Psest_0839 at 909.919 kb on + strand, within Psest_0839 at 909.919 kb on + strand, within Psest_0839 at 909.920 kb on - strand, within Psest_0839 at 909.920 kb on - strand, within Psest_0839 at 910.035 kb on + strand at 910.050 kb on + strand at 910.051 kb on - strand at 910.051 kb on - strand at 910.188 kb on + strand at 910.189 kb on - strand at 910.189 kb on - strand at 910.215 kb on + strand at 910.215 kb on + strand at 910.215 kb on + strand at 910.215 kb on + strand at 910.216 kb on - strand at 910.219 kb on + strand at 910.219 kb on + strand at 910.219 kb on + strand at 910.219 kb on + strand at 910.220 kb on - strand at 910.502 kb on + strand, within Psest_0840 at 910.502 kb on + strand, within Psest_0840 at 910.503 kb on - strand, within Psest_0840 at 910.503 kb on - strand, within Psest_0840 at 910.503 kb on - strand, within Psest_0840 at 910.503 kb on - strand, within Psest_0840 at 910.573 kb on + strand, within Psest_0840 at 910.573 kb on + strand, within Psest_0840 at 910.574 kb on - strand, within Psest_0840 at 910.584 kb on - strand, within Psest_0840 at 910.584 kb on - strand, within Psest_0840 at 910.584 kb on - strand, within Psest_0840 at 910.673 kb on - strand, within Psest_0840 at 910.673 kb on - strand, within Psest_0840 at 910.682 kb on + strand, within Psest_0840 at 910.682 kb on + strand, within Psest_0840 at 910.682 kb on + strand, within Psest_0840 at 910.682 kb on + strand, within Psest_0840 at 910.682 kb on + strand, within Psest_0840 at 910.683 kb on - strand, within Psest_0840 at 910.683 kb on - strand, within Psest_0840 at 910.700 kb on + strand, within Psest_0840 at 910.701 kb on - strand, within Psest_0840 at 910.744 kb on + strand, within Psest_0840 at 910.946 kb on - strand, within Psest_0840 at 910.949 kb on + strand, within Psest_0840 at 910.949 kb on + strand, within Psest_0840 at 910.949 kb on + strand, within Psest_0840 at 910.949 kb on + strand, within Psest_0840 at 910.949 kb on + strand, within Psest_0840 at 910.950 kb on - strand, within Psest_0840 at 910.950 kb on - strand, within Psest_0840 at 910.950 kb on - strand, within Psest_0840 at 910.950 kb on - strand, within Psest_0840
Per-strain Table
Position Strand Gene LocusTag Fraction Def A22 0.015 mg/ml remove 908,764 + +0.7 908,764 + -1.0 908,764 + +1.4 908,764 + -0.1 908,764 + -0.9 908,764 + -0.9 908,764 + -0.5 908,764 + +0.2 908,765 - -0.9 908,765 - -2.3 908,765 - -0.0 908,765 - +1.0 908,765 - +0.8 908,765 - +2.1 908,765 - -0.3 908,765 - +0.0 908,765 - +0.5 908,765 - -0.1 908,842 + -0.2 908,842 + -0.2 908,842 + -1.3 908,842 + -0.1 908,842 + -0.2 908,842 + -0.2 908,843 - -1.5 908,843 - +0.2 908,843 - -0.6 908,843 - -0.0 908,843 - +0.4 908,843 - -0.9 908,843 - -0.0 909,202 - -0.6 909,721 - Psest_0839 0.58 +1.5 909,846 + Psest_0839 0.76 -0.5 909,846 + Psest_0839 0.76 -0.8 909,847 - Psest_0839 0.76 -0.7 909,847 - Psest_0839 0.76 -0.8 909,847 - Psest_0839 0.76 -1.6 909,847 - Psest_0839 0.76 -0.5 909,847 - Psest_0839 0.76 -0.0 909,847 - Psest_0839 0.76 -0.6 909,919 + Psest_0839 0.87 -1.0 909,919 + Psest_0839 0.87 +0.5 909,919 + Psest_0839 0.87 +0.1 909,919 + Psest_0839 0.87 -0.6 909,919 + Psest_0839 0.87 +0.5 909,919 + Psest_0839 0.87 -1.5 909,920 - Psest_0839 0.87 -0.8 909,920 - Psest_0839 0.87 -0.6 910,035 + +0.5 910,050 + -0.6 910,051 - -0.8 910,051 - -1.3 910,188 + +2.1 910,189 - -0.8 910,189 - -0.0 910,215 + +0.1 910,215 + +0.5 910,215 + -0.5 910,215 + +0.2 910,216 - -1.2 910,219 + +1.2 910,219 + -1.2 910,219 + -1.4 910,219 + +0.4 910,220 - -1.3 910,502 + Psest_0840 0.13 -2.2 910,502 + Psest_0840 0.13 -0.1 910,503 - Psest_0840 0.13 -1.8 910,503 - Psest_0840 0.13 +0.1 910,503 - Psest_0840 0.13 -0.5 910,503 - Psest_0840 0.13 -1.2 910,573 + Psest_0840 0.17 -0.5 910,573 + Psest_0840 0.17 -1.7 910,574 - Psest_0840 0.17 +0.3 910,584 - Psest_0840 0.17 -0.3 910,584 - Psest_0840 0.17 -0.4 910,584 - Psest_0840 0.17 +0.3 910,673 - Psest_0840 0.22 -0.2 910,673 - Psest_0840 0.22 -0.3 910,682 + Psest_0840 0.22 -0.4 910,682 + Psest_0840 0.22 +0.9 910,682 + Psest_0840 0.22 +0.0 910,682 + Psest_0840 0.22 -0.8 910,682 + Psest_0840 0.22 +1.0 910,683 - Psest_0840 0.22 -0.1 910,683 - Psest_0840 0.22 -0.2 910,700 + Psest_0840 0.23 +0.1 910,701 - Psest_0840 0.23 +1.2 910,744 + Psest_0840 0.25 +0.7 910,946 - Psest_0840 0.35 -0.2 910,949 + Psest_0840 0.35 -0.1 910,949 + Psest_0840 0.35 -0.4 910,949 + Psest_0840 0.35 -0.0 910,949 + Psest_0840 0.35 +1.0 910,949 + Psest_0840 0.35 +0.0 910,950 - Psest_0840 0.35 -0.8 910,950 - Psest_0840 0.35 -1.2 910,950 - Psest_0840 0.35 -0.6 910,950 - Psest_0840 0.35 +2.0
Or see this region's nucleotide sequence