Experiment: Def A22 0.015 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2356 and Psest_2357 are separated by 1 nucleotides Psest_2357 and Psest_2358 overlap by 1 nucleotides Psest_2358 and Psest_2359 overlap by 4 nucleotides Psest_2359 and Psest_2360 overlap by 4 nucleotides
Psest_2356: Psest_2356 - Fatty acid desaturase, at 2,511,479 to 2,512,501
_2356
Psest_2357: Psest_2357 - Short-chain dehydrogenases of various substrate specificities, at 2,512,503 to 2,513,291
_2357
Psest_2358: Psest_2358 - Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases), at 2,513,291 to 2,514,049
_2358
Psest_2359: Psest_2359 - Predicted permeases, at 2,514,046 to 2,514,930
_2359
Psest_2360: Psest_2360 - Uncharacterized protein, putative amidase, at 2,514,927 to 2,515,688
_2360
Position (kb)
2513
2514
2515 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2512.331 kb on + strand, within Psest_2356 at 2512.343 kb on + strand, within Psest_2356 at 2512.343 kb on + strand, within Psest_2356 at 2512.343 kb on + strand, within Psest_2356 at 2512.343 kb on + strand, within Psest_2356 at 2512.343 kb on + strand, within Psest_2356 at 2512.343 kb on + strand, within Psest_2356 at 2512.343 kb on + strand, within Psest_2356 at 2512.343 kb on + strand, within Psest_2356 at 2512.343 kb on + strand, within Psest_2356 at 2512.344 kb on - strand, within Psest_2356 at 2512.344 kb on - strand, within Psest_2356 at 2512.344 kb on - strand, within Psest_2356 at 2512.464 kb on - strand at 2512.522 kb on - strand at 2512.522 kb on - strand at 2512.522 kb on - strand at 2512.725 kb on + strand, within Psest_2357 at 2512.975 kb on - strand, within Psest_2357 at 2512.975 kb on - strand, within Psest_2357 at 2512.975 kb on - strand, within Psest_2357 at 2512.996 kb on + strand, within Psest_2357 at 2513.751 kb on - strand, within Psest_2358 at 2513.751 kb on - strand, within Psest_2358 at 2513.751 kb on - strand, within Psest_2358 at 2513.996 kb on + strand at 2513.996 kb on + strand at 2513.996 kb on + strand at 2513.996 kb on + strand at 2513.997 kb on - strand at 2514.169 kb on + strand, within Psest_2359 at 2514.169 kb on + strand, within Psest_2359 at 2514.170 kb on - strand, within Psest_2359 at 2514.337 kb on + strand, within Psest_2359 at 2514.337 kb on + strand, within Psest_2359 at 2514.338 kb on - strand, within Psest_2359 at 2514.338 kb on - strand, within Psest_2359 at 2514.338 kb on - strand, within Psest_2359
Per-strain Table
Position Strand Gene LocusTag Fraction Def A22 0.015 mg/ml remove 2,512,331 + Psest_2356 0.83 -0.7 2,512,343 + Psest_2356 0.84 +0.2 2,512,343 + Psest_2356 0.84 -0.1 2,512,343 + Psest_2356 0.84 +1.3 2,512,343 + Psest_2356 0.84 -0.7 2,512,343 + Psest_2356 0.84 -0.2 2,512,343 + Psest_2356 0.84 +0.2 2,512,343 + Psest_2356 0.84 +0.3 2,512,343 + Psest_2356 0.84 +0.3 2,512,343 + Psest_2356 0.84 +0.3 2,512,344 - Psest_2356 0.85 -0.1 2,512,344 - Psest_2356 0.85 +0.2 2,512,344 - Psest_2356 0.85 +0.3 2,512,464 - +0.3 2,512,522 - -0.1 2,512,522 - +0.6 2,512,522 - -1.7 2,512,725 + Psest_2357 0.28 -0.1 2,512,975 - Psest_2357 0.60 +0.0 2,512,975 - Psest_2357 0.60 +0.1 2,512,975 - Psest_2357 0.60 +0.6 2,512,996 + Psest_2357 0.62 +1.0 2,513,751 - Psest_2358 0.61 -1.5 2,513,751 - Psest_2358 0.61 -0.9 2,513,751 - Psest_2358 0.61 -0.2 2,513,996 + +0.3 2,513,996 + -0.7 2,513,996 + -0.3 2,513,996 + -2.2 2,513,997 - +0.7 2,514,169 + Psest_2359 0.14 +0.2 2,514,169 + Psest_2359 0.14 -0.2 2,514,170 - Psest_2359 0.14 -0.7 2,514,337 + Psest_2359 0.33 +1.3 2,514,337 + Psest_2359 0.33 +0.1 2,514,338 - Psest_2359 0.33 +3.3 2,514,338 - Psest_2359 0.33 -0.9 2,514,338 - Psest_2359 0.33 -0.2
Or see this region's nucleotide sequence