Experiment: Def A22 0.015 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0106 and Psest_0107 are separated by 72 nucleotides Psest_0107 and Psest_0108 overlap by 4 nucleotides Psest_0108 and Psest_0109 are separated by 124 nucleotides Psest_0109 and Psest_0110 are separated by 5 nucleotides
Psest_0106: Psest_0106 - malonate decarboxylase, gamma subunit, at 105,053 to 105,838
_0106
Psest_0107: Psest_0107 - malonate decarboxylase holo-[acyl-carrier-protein] synthase, at 105,911 to 106,528
_0107
Psest_0108: Psest_0108 - malonate decarboxylase, epsilon subunit, at 106,525 to 107,451
_0108
Psest_0109: Psest_0109 - malonate transporter, MadL subunit, at 107,576 to 107,998
_0109
Psest_0110: Psest_0110 - malonate transporter, MadM subunit, at 108,004 to 108,768
_0110
Position (kb)
106
107
108 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 105.621 kb on - strand, within Psest_0106 at 105.635 kb on + strand, within Psest_0106 at 105.635 kb on + strand, within Psest_0106 at 105.636 kb on - strand, within Psest_0106 at 105.636 kb on - strand, within Psest_0106 at 105.636 kb on - strand, within Psest_0106 at 105.636 kb on - strand, within Psest_0106 at 105.636 kb on - strand, within Psest_0106 at 105.908 kb on + strand at 105.908 kb on + strand at 105.908 kb on + strand at 105.909 kb on - strand at 105.909 kb on - strand at 105.909 kb on - strand at 105.909 kb on - strand at 106.230 kb on - strand, within Psest_0107 at 106.765 kb on + strand, within Psest_0108 at 106.765 kb on + strand, within Psest_0108 at 106.765 kb on + strand, within Psest_0108 at 106.765 kb on + strand, within Psest_0108 at 106.765 kb on + strand, within Psest_0108 at 106.765 kb on + strand, within Psest_0108 at 106.765 kb on + strand, within Psest_0108 at 106.765 kb on + strand, within Psest_0108 at 106.766 kb on - strand, within Psest_0108 at 106.766 kb on - strand, within Psest_0108 at 106.766 kb on - strand, within Psest_0108 at 106.766 kb on - strand, within Psest_0108 at 106.766 kb on - strand, within Psest_0108 at 106.792 kb on + strand, within Psest_0108 at 106.792 kb on + strand, within Psest_0108 at 106.792 kb on + strand, within Psest_0108 at 106.826 kb on - strand, within Psest_0108 at 106.826 kb on - strand, within Psest_0108 at 106.826 kb on - strand, within Psest_0108 at 106.879 kb on + strand, within Psest_0108 at 106.966 kb on + strand, within Psest_0108 at 106.977 kb on + strand, within Psest_0108 at 106.977 kb on + strand, within Psest_0108 at 107.180 kb on - strand, within Psest_0108 at 107.309 kb on + strand, within Psest_0108 at 107.373 kb on + strand at 107.449 kb on + strand at 107.514 kb on + strand at 107.514 kb on + strand at 107.514 kb on + strand at 107.514 kb on + strand at 107.515 kb on - strand at 107.515 kb on - strand at 107.515 kb on - strand at 107.515 kb on - strand at 107.515 kb on - strand at 107.515 kb on - strand at 107.720 kb on + strand, within Psest_0109 at 107.720 kb on + strand, within Psest_0109 at 107.720 kb on + strand, within Psest_0109 at 107.720 kb on + strand, within Psest_0109 at 107.786 kb on + strand, within Psest_0109 at 107.786 kb on + strand, within Psest_0109 at 107.786 kb on + strand, within Psest_0109 at 107.786 kb on + strand, within Psest_0109 at 107.786 kb on + strand, within Psest_0109 at 107.786 kb on + strand, within Psest_0109 at 107.786 kb on + strand, within Psest_0109 at 107.786 kb on + strand, within Psest_0109 at 107.786 kb on + strand, within Psest_0109 at 107.786 kb on + strand, within Psest_0109 at 107.786 kb on + strand, within Psest_0109 at 107.787 kb on - strand, within Psest_0109 at 107.787 kb on - strand, within Psest_0109 at 107.787 kb on - strand, within Psest_0109 at 107.787 kb on - strand, within Psest_0109 at 107.787 kb on - strand, within Psest_0109 at 107.787 kb on - strand, within Psest_0109 at 107.787 kb on - strand, within Psest_0109 at 107.787 kb on - strand, within Psest_0109 at 107.828 kb on + strand, within Psest_0109 at 108.007 kb on + strand at 108.007 kb on + strand at 108.007 kb on + strand at 108.007 kb on + strand at 108.007 kb on + strand at 108.007 kb on + strand at 108.007 kb on + strand at 108.008 kb on - strand at 108.008 kb on - strand at 108.008 kb on - strand at 108.008 kb on - strand at 108.038 kb on - strand at 108.089 kb on - strand, within Psest_0110 at 108.089 kb on - strand, within Psest_0110 at 108.166 kb on + strand, within Psest_0110 at 108.166 kb on + strand, within Psest_0110 at 108.166 kb on + strand, within Psest_0110 at 108.166 kb on + strand, within Psest_0110 at 108.166 kb on + strand, within Psest_0110 at 108.166 kb on + strand, within Psest_0110 at 108.166 kb on + strand, within Psest_0110 at 108.166 kb on + strand, within Psest_0110 at 108.166 kb on + strand, within Psest_0110 at 108.166 kb on + strand, within Psest_0110 at 108.166 kb on + strand, within Psest_0110 at 108.166 kb on + strand, within Psest_0110 at 108.166 kb on + strand, within Psest_0110 at 108.166 kb on + strand, within Psest_0110 at 108.166 kb on + strand, within Psest_0110 at 108.166 kb on + strand, within Psest_0110 at 108.167 kb on - strand, within Psest_0110 at 108.167 kb on - strand, within Psest_0110 at 108.167 kb on - strand, within Psest_0110 at 108.167 kb on - strand, within Psest_0110 at 108.167 kb on - strand, within Psest_0110 at 108.167 kb on - strand, within Psest_0110 at 108.167 kb on - strand, within Psest_0110 at 108.167 kb on - strand, within Psest_0110 at 108.167 kb on - strand, within Psest_0110 at 108.167 kb on - strand, within Psest_0110 at 108.167 kb on - strand, within Psest_0110 at 108.167 kb on - strand, within Psest_0110 at 108.167 kb on - strand, within Psest_0110 at 108.167 kb on - strand, within Psest_0110 at 108.167 kb on - strand, within Psest_0110 at 108.167 kb on - strand, within Psest_0110 at 108.167 kb on - strand, within Psest_0110 at 108.167 kb on - strand, within Psest_0110 at 108.167 kb on - strand, within Psest_0110 at 108.398 kb on - strand, within Psest_0110 at 108.445 kb on + strand, within Psest_0110 at 108.445 kb on + strand, within Psest_0110 at 108.445 kb on + strand, within Psest_0110 at 108.445 kb on + strand, within Psest_0110 at 108.445 kb on + strand, within Psest_0110 at 108.445 kb on + strand, within Psest_0110 at 108.445 kb on + strand, within Psest_0110 at 108.445 kb on + strand, within Psest_0110 at 108.445 kb on + strand, within Psest_0110 at 108.446 kb on - strand, within Psest_0110 at 108.446 kb on - strand, within Psest_0110 at 108.446 kb on - strand, within Psest_0110 at 108.446 kb on - strand, within Psest_0110 at 108.446 kb on - strand, within Psest_0110 at 108.446 kb on - strand, within Psest_0110
Per-strain Table
Position Strand Gene LocusTag Fraction Def A22 0.015 mg/ml remove 105,621 - Psest_0106 0.72 +0.9 105,635 + Psest_0106 0.74 +0.8 105,635 + Psest_0106 0.74 +1.3 105,636 - Psest_0106 0.74 +0.2 105,636 - Psest_0106 0.74 +0.9 105,636 - Psest_0106 0.74 +0.3 105,636 - Psest_0106 0.74 +0.7 105,636 - Psest_0106 0.74 -0.7 105,908 + +0.9 105,908 + +0.2 105,908 + -0.4 105,909 - +1.9 105,909 - +1.2 105,909 - +0.7 105,909 - +1.2 106,230 - Psest_0107 0.52 +0.9 106,765 + Psest_0108 0.26 -0.7 106,765 + Psest_0108 0.26 -0.0 106,765 + Psest_0108 0.26 -0.7 106,765 + Psest_0108 0.26 -0.7 106,765 + Psest_0108 0.26 +0.1 106,765 + Psest_0108 0.26 +0.1 106,765 + Psest_0108 0.26 +1.9 106,765 + Psest_0108 0.26 -0.5 106,766 - Psest_0108 0.26 +1.9 106,766 - Psest_0108 0.26 +3.5 106,766 - Psest_0108 0.26 +0.6 106,766 - Psest_0108 0.26 +0.6 106,766 - Psest_0108 0.26 +1.2 106,792 + Psest_0108 0.29 +0.9 106,792 + Psest_0108 0.29 -0.9 106,792 + Psest_0108 0.29 +0.9 106,826 - Psest_0108 0.32 +2.9 106,826 - Psest_0108 0.32 +1.9 106,826 - Psest_0108 0.32 +0.9 106,879 + Psest_0108 0.38 -0.1 106,966 + Psest_0108 0.48 +2.2 106,977 + Psest_0108 0.49 +2.5 106,977 + Psest_0108 0.49 +1.3 107,180 - Psest_0108 0.71 +0.3 107,309 + Psest_0108 0.85 -0.5 107,373 + +0.9 107,449 + -0.8 107,514 + +0.3 107,514 + -0.3 107,514 + -0.6 107,514 + +0.6 107,515 - +0.1 107,515 - +1.3 107,515 - +0.9 107,515 - -1.4 107,515 - +2.9 107,515 - +0.6 107,720 + Psest_0109 0.34 +1.2 107,720 + Psest_0109 0.34 +0.3 107,720 + Psest_0109 0.34 +0.6 107,720 + Psest_0109 0.34 +0.0 107,786 + Psest_0109 0.50 -0.1 107,786 + Psest_0109 0.50 -0.4 107,786 + Psest_0109 0.50 +0.1 107,786 + Psest_0109 0.50 +0.1 107,786 + Psest_0109 0.50 +0.3 107,786 + Psest_0109 0.50 +0.7 107,786 + Psest_0109 0.50 -1.3 107,786 + Psest_0109 0.50 -1.3 107,786 + Psest_0109 0.50 -0.3 107,786 + Psest_0109 0.50 -0.9 107,786 + Psest_0109 0.50 -0.8 107,787 - Psest_0109 0.50 +0.1 107,787 - Psest_0109 0.50 -0.6 107,787 - Psest_0109 0.50 -1.1 107,787 - Psest_0109 0.50 -0.7 107,787 - Psest_0109 0.50 +2.5 107,787 - Psest_0109 0.50 +0.3 107,787 - Psest_0109 0.50 +0.2 107,787 - Psest_0109 0.50 +0.3 107,828 + Psest_0109 0.60 +0.2 108,007 + +0.3 108,007 + -0.8 108,007 + +1.3 108,007 + +2.9 108,007 + +1.5 108,007 + +0.4 108,007 + -0.1 108,008 - -0.7 108,008 - -0.9 108,008 - +0.9 108,008 - -1.7 108,038 - +0.9 108,089 - Psest_0110 0.11 -0.1 108,089 - Psest_0110 0.11 -0.3 108,166 + Psest_0110 0.21 +0.8 108,166 + Psest_0110 0.21 +0.9 108,166 + Psest_0110 0.21 -0.1 108,166 + Psest_0110 0.21 +0.3 108,166 + Psest_0110 0.21 -0.9 108,166 + Psest_0110 0.21 -2.0 108,166 + Psest_0110 0.21 +0.9 108,166 + Psest_0110 0.21 +1.2 108,166 + Psest_0110 0.21 -0.4 108,166 + Psest_0110 0.21 +0.9 108,166 + Psest_0110 0.21 +0.4 108,166 + Psest_0110 0.21 +0.5 108,166 + Psest_0110 0.21 -1.1 108,166 + Psest_0110 0.21 +0.4 108,166 + Psest_0110 0.21 -0.7 108,166 + Psest_0110 0.21 -1.0 108,167 - Psest_0110 0.21 -0.2 108,167 - Psest_0110 0.21 +2.9 108,167 - Psest_0110 0.21 +1.5 108,167 - Psest_0110 0.21 +1.9 108,167 - Psest_0110 0.21 -2.5 108,167 - Psest_0110 0.21 +1.9 108,167 - Psest_0110 0.21 +0.0 108,167 - Psest_0110 0.21 +1.3 108,167 - Psest_0110 0.21 -0.6 108,167 - Psest_0110 0.21 +1.5 108,167 - Psest_0110 0.21 -0.7 108,167 - Psest_0110 0.21 -0.4 108,167 - Psest_0110 0.21 +0.3 108,167 - Psest_0110 0.21 -0.3 108,167 - Psest_0110 0.21 +0.9 108,167 - Psest_0110 0.21 -0.1 108,167 - Psest_0110 0.21 +0.6 108,167 - Psest_0110 0.21 -0.4 108,167 - Psest_0110 0.21 -0.7 108,398 - Psest_0110 0.52 +0.6 108,445 + Psest_0110 0.58 -1.2 108,445 + Psest_0110 0.58 +0.9 108,445 + Psest_0110 0.58 +0.1 108,445 + Psest_0110 0.58 +0.7 108,445 + Psest_0110 0.58 +0.3 108,445 + Psest_0110 0.58 +1.2 108,445 + Psest_0110 0.58 +0.2 108,445 + Psest_0110 0.58 -0.1 108,445 + Psest_0110 0.58 -0.7 108,446 - Psest_0110 0.58 -1.0 108,446 - Psest_0110 0.58 +0.9 108,446 - Psest_0110 0.58 +0.3 108,446 - Psest_0110 0.58 +0.1 108,446 - Psest_0110 0.58 -1.4 108,446 - Psest_0110 0.58 -0.9
Or see this region's nucleotide sequence