Experiment: Def Cephalothin 0.08 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0532 and Psest_0533 are separated by 97 nucleotides Psest_0533 and Psest_0534 are separated by 117 nucleotides Psest_0534 and Psest_0535 are separated by 8 nucleotides
Psest_0532: Psest_0532 - tRNA-N(6)-(isopentenyl)adenosine-37 thiotransferase enzyme MiaB, at 585,288 to 586,601
_0532
Psest_0533: Psest_0533 - Sel1 repeat., at 586,699 to 587,235
_0533
Psest_0534: Psest_0534 - hypothetical protein, at 587,353 to 587,976
_0534
Psest_0535: Psest_0535 - glutamate-1-semialdehyde-2,1-aminomutase, at 587,985 to 589,268
_0535
Position (kb)
587
588 Strain fitness (log2 ratio)
-1
0
1
2
3 at 586.460 kb on + strand, within Psest_0532 at 586.461 kb on - strand, within Psest_0532 at 586.641 kb on + strand at 586.641 kb on + strand at 586.641 kb on + strand at 586.641 kb on + strand at 586.641 kb on + strand at 586.642 kb on - strand at 586.642 kb on - strand at 586.642 kb on - strand at 586.642 kb on - strand at 586.875 kb on - strand, within Psest_0533 at 586.875 kb on - strand, within Psest_0533 at 586.875 kb on - strand, within Psest_0533 at 586.875 kb on - strand, within Psest_0533 at 586.875 kb on - strand, within Psest_0533 at 586.875 kb on - strand, within Psest_0533 at 586.875 kb on - strand, within Psest_0533 at 586.875 kb on - strand, within Psest_0533 at 586.875 kb on - strand, within Psest_0533 at 586.875 kb on - strand, within Psest_0533 at 586.875 kb on - strand, within Psest_0533 at 586.875 kb on - strand, within Psest_0533 at 586.979 kb on + strand, within Psest_0533 at 586.979 kb on + strand, within Psest_0533 at 586.979 kb on + strand, within Psest_0533 at 586.979 kb on + strand, within Psest_0533 at 586.979 kb on + strand, within Psest_0533 at 586.979 kb on + strand, within Psest_0533 at 586.979 kb on + strand, within Psest_0533 at 586.980 kb on - strand, within Psest_0533 at 587.021 kb on + strand, within Psest_0533 at 587.043 kb on - strand, within Psest_0533 at 587.299 kb on + strand at 587.300 kb on - strand at 587.321 kb on + strand at 587.321 kb on + strand at 587.321 kb on + strand at 587.321 kb on + strand at 587.321 kb on + strand at 587.321 kb on + strand at 587.322 kb on - strand at 587.322 kb on - strand at 587.354 kb on + strand at 587.355 kb on - strand at 587.573 kb on + strand, within Psest_0534 at 587.573 kb on + strand, within Psest_0534 at 587.573 kb on + strand, within Psest_0534 at 587.573 kb on + strand, within Psest_0534 at 587.573 kb on + strand, within Psest_0534 at 587.574 kb on - strand, within Psest_0534 at 587.616 kb on - strand, within Psest_0534
Per-strain Table
Position Strand Gene LocusTag Fraction Def Cephalothin 0.08 mg/ml remove 586,460 + Psest_0532 0.89 -0.6 586,461 - Psest_0532 0.89 +0.1 586,641 + +2.0 586,641 + +0.9 586,641 + +1.1 586,641 + -0.4 586,641 + +0.6 586,642 - -1.2 586,642 - -0.7 586,642 - +0.4 586,642 - +0.9 586,875 - Psest_0533 0.33 +0.7 586,875 - Psest_0533 0.33 +0.4 586,875 - Psest_0533 0.33 -1.4 586,875 - Psest_0533 0.33 +1.5 586,875 - Psest_0533 0.33 +1.0 586,875 - Psest_0533 0.33 +1.2 586,875 - Psest_0533 0.33 -0.1 586,875 - Psest_0533 0.33 +0.0 586,875 - Psest_0533 0.33 +3.3 586,875 - Psest_0533 0.33 +1.6 586,875 - Psest_0533 0.33 +1.4 586,875 - Psest_0533 0.33 -0.2 586,979 + Psest_0533 0.52 +0.3 586,979 + Psest_0533 0.52 +2.7 586,979 + Psest_0533 0.52 +0.7 586,979 + Psest_0533 0.52 -1.3 586,979 + Psest_0533 0.52 +1.7 586,979 + Psest_0533 0.52 -0.4 586,979 + Psest_0533 0.52 +1.3 586,980 - Psest_0533 0.52 -0.3 587,021 + Psest_0533 0.60 -0.4 587,043 - Psest_0533 0.64 +1.7 587,299 + -0.1 587,300 - -0.6 587,321 + +1.7 587,321 + +1.2 587,321 + +1.1 587,321 + -0.9 587,321 + +1.7 587,321 + +1.7 587,322 - -0.8 587,322 - +0.4 587,354 + +0.7 587,355 - -0.2 587,573 + Psest_0534 0.35 -0.4 587,573 + Psest_0534 0.35 +0.7 587,573 + Psest_0534 0.35 -0.9 587,573 + Psest_0534 0.35 -1.4 587,573 + Psest_0534 0.35 -1.3 587,574 - Psest_0534 0.35 -0.1 587,616 - Psest_0534 0.42 -1.4
Or see this region's nucleotide sequence