Experiment: Def Cephalothin 0.08 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1399 and Psest_1400 are separated by 50 nucleotides Psest_1400 and Psest_1401 overlap by 8 nucleotides Psest_1401 and Psest_1402 are separated by 302 nucleotides
Psest_1399: Psest_1399 - ABC-type nitrate/sulfonate/bicarbonate transport systems, periplasmic components, at 1,506,771 to 1,507,748
_1399
Psest_1400: Psest_1400 - Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily), at 1,507,799 to 1,508,548
_1400
Psest_1401: Psest_1401 - Isopenicillin N synthase and related dioxygenases, at 1,508,541 to 1,509,521
_1401
Psest_1402: Psest_1402 - Putative threonine efflux protein, at 1,509,824 to 1,510,432
_1402
Position (kb)
1508
1509
1510 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1507.623 kb on + strand, within Psest_1399 at 1507.623 kb on + strand, within Psest_1399 at 1507.623 kb on + strand, within Psest_1399 at 1507.623 kb on + strand, within Psest_1399 at 1507.623 kb on + strand, within Psest_1399 at 1507.624 kb on - strand, within Psest_1399 at 1507.624 kb on - strand, within Psest_1399 at 1507.624 kb on - strand, within Psest_1399 at 1507.624 kb on - strand, within Psest_1399 at 1507.624 kb on - strand, within Psest_1399 at 1507.624 kb on - strand, within Psest_1399 at 1507.627 kb on + strand, within Psest_1399 at 1507.627 kb on + strand, within Psest_1399 at 1507.627 kb on + strand, within Psest_1399 at 1507.627 kb on + strand, within Psest_1399 at 1507.627 kb on + strand, within Psest_1399 at 1507.627 kb on + strand, within Psest_1399 at 1507.627 kb on + strand, within Psest_1399 at 1507.628 kb on - strand, within Psest_1399 at 1507.628 kb on - strand, within Psest_1399 at 1507.628 kb on - strand, within Psest_1399 at 1507.628 kb on - strand, within Psest_1399 at 1507.628 kb on - strand, within Psest_1399 at 1507.628 kb on - strand, within Psest_1399 at 1507.791 kb on + strand at 1507.980 kb on + strand, within Psest_1400 at 1507.980 kb on + strand, within Psest_1400 at 1507.980 kb on + strand, within Psest_1400 at 1507.981 kb on - strand, within Psest_1400 at 1507.981 kb on - strand, within Psest_1400 at 1507.981 kb on - strand, within Psest_1400 at 1508.259 kb on + strand, within Psest_1400 at 1508.259 kb on + strand, within Psest_1400 at 1508.259 kb on + strand, within Psest_1400 at 1508.286 kb on + strand, within Psest_1400 at 1508.286 kb on + strand, within Psest_1400 at 1508.287 kb on - strand, within Psest_1400 at 1508.287 kb on - strand, within Psest_1400 at 1508.287 kb on - strand, within Psest_1400 at 1508.287 kb on - strand, within Psest_1400 at 1508.287 kb on - strand, within Psest_1400 at 1508.388 kb on + strand, within Psest_1400 at 1508.388 kb on + strand, within Psest_1400 at 1508.389 kb on - strand, within Psest_1400 at 1508.548 kb on + strand at 1508.548 kb on + strand at 1508.548 kb on + strand at 1508.548 kb on + strand at 1508.548 kb on + strand at 1508.549 kb on - strand at 1508.549 kb on - strand at 1508.549 kb on - strand at 1508.684 kb on + strand, within Psest_1401 at 1508.685 kb on - strand, within Psest_1401 at 1508.685 kb on - strand, within Psest_1401 at 1508.685 kb on - strand, within Psest_1401 at 1508.721 kb on + strand, within Psest_1401 at 1508.722 kb on - strand, within Psest_1401 at 1508.782 kb on + strand, within Psest_1401 at 1508.782 kb on + strand, within Psest_1401 at 1508.782 kb on + strand, within Psest_1401 at 1508.782 kb on + strand, within Psest_1401 at 1508.782 kb on + strand, within Psest_1401 at 1508.782 kb on + strand, within Psest_1401 at 1508.782 kb on + strand, within Psest_1401 at 1508.782 kb on + strand, within Psest_1401 at 1508.782 kb on + strand, within Psest_1401 at 1508.782 kb on + strand, within Psest_1401 at 1508.782 kb on + strand, within Psest_1401 at 1508.782 kb on + strand, within Psest_1401 at 1508.782 kb on + strand, within Psest_1401 at 1508.782 kb on + strand, within Psest_1401 at 1508.782 kb on + strand, within Psest_1401 at 1508.782 kb on + strand, within Psest_1401 at 1508.782 kb on + strand, within Psest_1401 at 1508.782 kb on + strand, within Psest_1401 at 1508.783 kb on - strand, within Psest_1401 at 1508.783 kb on - strand, within Psest_1401 at 1508.783 kb on - strand, within Psest_1401 at 1508.783 kb on - strand, within Psest_1401 at 1508.783 kb on - strand, within Psest_1401 at 1508.783 kb on - strand, within Psest_1401 at 1508.783 kb on - strand, within Psest_1401 at 1508.783 kb on - strand, within Psest_1401 at 1508.783 kb on - strand, within Psest_1401 at 1508.783 kb on - strand, within Psest_1401 at 1508.783 kb on - strand, within Psest_1401 at 1508.783 kb on - strand, within Psest_1401 at 1508.783 kb on - strand, within Psest_1401 at 1509.184 kb on + strand, within Psest_1401 at 1509.184 kb on + strand, within Psest_1401 at 1509.184 kb on + strand, within Psest_1401 at 1509.184 kb on + strand, within Psest_1401 at 1509.185 kb on - strand, within Psest_1401 at 1509.185 kb on - strand, within Psest_1401 at 1509.185 kb on - strand, within Psest_1401 at 1509.185 kb on - strand, within Psest_1401 at 1509.185 kb on - strand, within Psest_1401 at 1509.185 kb on - strand, within Psest_1401 at 1509.185 kb on - strand, within Psest_1401 at 1509.185 kb on - strand, within Psest_1401 at 1509.185 kb on - strand, within Psest_1401 at 1509.185 kb on - strand, within Psest_1401 at 1509.247 kb on + strand, within Psest_1401 at 1509.569 kb on + strand at 1509.569 kb on + strand at 1509.569 kb on + strand at 1509.642 kb on - strand at 1509.642 kb on - strand at 1509.734 kb on + strand at 1509.735 kb on - strand at 1509.735 kb on - strand at 1510.004 kb on + strand, within Psest_1402 at 1510.004 kb on + strand, within Psest_1402 at 1510.004 kb on + strand, within Psest_1402 at 1510.004 kb on + strand, within Psest_1402 at 1510.005 kb on - strand, within Psest_1402 at 1510.005 kb on - strand, within Psest_1402 at 1510.005 kb on - strand, within Psest_1402 at 1510.005 kb on - strand, within Psest_1402 at 1510.035 kb on + strand, within Psest_1402 at 1510.035 kb on + strand, within Psest_1402 at 1510.035 kb on + strand, within Psest_1402 at 1510.035 kb on + strand, within Psest_1402 at 1510.035 kb on + strand, within Psest_1402 at 1510.036 kb on - strand, within Psest_1402 at 1510.036 kb on - strand, within Psest_1402 at 1510.036 kb on - strand, within Psest_1402 at 1510.036 kb on - strand, within Psest_1402 at 1510.036 kb on - strand, within Psest_1402 at 1510.036 kb on - strand, within Psest_1402 at 1510.036 kb on - strand, within Psest_1402 at 1510.103 kb on + strand, within Psest_1402 at 1510.103 kb on + strand, within Psest_1402 at 1510.103 kb on + strand, within Psest_1402 at 1510.103 kb on + strand, within Psest_1402 at 1510.103 kb on + strand, within Psest_1402 at 1510.104 kb on - strand, within Psest_1402 at 1510.104 kb on - strand, within Psest_1402 at 1510.104 kb on - strand, within Psest_1402 at 1510.104 kb on - strand, within Psest_1402 at 1510.130 kb on + strand, within Psest_1402 at 1510.131 kb on - strand, within Psest_1402 at 1510.131 kb on - strand, within Psest_1402 at 1510.131 kb on - strand, within Psest_1402 at 1510.183 kb on + strand, within Psest_1402 at 1510.184 kb on - strand, within Psest_1402 at 1510.184 kb on - strand, within Psest_1402 at 1510.184 kb on - strand, within Psest_1402 at 1510.185 kb on + strand, within Psest_1402 at 1510.185 kb on + strand, within Psest_1402 at 1510.185 kb on + strand, within Psest_1402 at 1510.186 kb on - strand, within Psest_1402 at 1510.436 kb on + strand at 1510.436 kb on + strand at 1510.436 kb on + strand at 1510.436 kb on + strand at 1510.436 kb on + strand at 1510.437 kb on - strand at 1510.437 kb on - strand at 1510.437 kb on - strand at 1510.437 kb on - strand at 1510.437 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Cephalothin 0.08 mg/ml remove 1,507,623 + Psest_1399 0.87 +0.2 1,507,623 + Psest_1399 0.87 -0.2 1,507,623 + Psest_1399 0.87 -0.9 1,507,623 + Psest_1399 0.87 -1.0 1,507,623 + Psest_1399 0.87 +3.6 1,507,624 - Psest_1399 0.87 +0.0 1,507,624 - Psest_1399 0.87 -0.1 1,507,624 - Psest_1399 0.87 -0.7 1,507,624 - Psest_1399 0.87 +0.8 1,507,624 - Psest_1399 0.87 -0.8 1,507,624 - Psest_1399 0.87 -1.2 1,507,627 + Psest_1399 0.88 -1.6 1,507,627 + Psest_1399 0.88 -0.4 1,507,627 + Psest_1399 0.88 +0.0 1,507,627 + Psest_1399 0.88 -2.7 1,507,627 + Psest_1399 0.88 +0.9 1,507,627 + Psest_1399 0.88 -2.5 1,507,627 + Psest_1399 0.88 -0.6 1,507,628 - Psest_1399 0.88 -0.4 1,507,628 - Psest_1399 0.88 +0.6 1,507,628 - Psest_1399 0.88 -1.0 1,507,628 - Psest_1399 0.88 -1.6 1,507,628 - Psest_1399 0.88 -0.6 1,507,628 - Psest_1399 0.88 -1.0 1,507,791 + +2.6 1,507,980 + Psest_1400 0.24 +0.2 1,507,980 + Psest_1400 0.24 -0.4 1,507,980 + Psest_1400 0.24 -0.5 1,507,981 - Psest_1400 0.24 -0.8 1,507,981 - Psest_1400 0.24 -0.3 1,507,981 - Psest_1400 0.24 +1.6 1,508,259 + Psest_1400 0.61 +0.7 1,508,259 + Psest_1400 0.61 -0.1 1,508,259 + Psest_1400 0.61 -0.8 1,508,286 + Psest_1400 0.65 -1.0 1,508,286 + Psest_1400 0.65 -0.3 1,508,287 - Psest_1400 0.65 -1.6 1,508,287 - Psest_1400 0.65 +0.0 1,508,287 - Psest_1400 0.65 +0.8 1,508,287 - Psest_1400 0.65 +0.0 1,508,287 - Psest_1400 0.65 -0.7 1,508,388 + Psest_1400 0.79 -1.7 1,508,388 + Psest_1400 0.79 +0.2 1,508,389 - Psest_1400 0.79 +1.2 1,508,548 + -0.9 1,508,548 + +0.9 1,508,548 + -0.3 1,508,548 + -0.8 1,508,548 + +0.2 1,508,549 - -0.8 1,508,549 - +0.0 1,508,549 - -0.3 1,508,684 + Psest_1401 0.15 -2.1 1,508,685 - Psest_1401 0.15 +2.0 1,508,685 - Psest_1401 0.15 +1.0 1,508,685 - Psest_1401 0.15 -0.4 1,508,721 + Psest_1401 0.18 +0.1 1,508,722 - Psest_1401 0.18 -1.2 1,508,782 + Psest_1401 0.25 -0.4 1,508,782 + Psest_1401 0.25 +0.1 1,508,782 + Psest_1401 0.25 +0.2 1,508,782 + Psest_1401 0.25 +0.8 1,508,782 + Psest_1401 0.25 -0.2 1,508,782 + Psest_1401 0.25 -0.2 1,508,782 + Psest_1401 0.25 +1.1 1,508,782 + Psest_1401 0.25 -1.8 1,508,782 + Psest_1401 0.25 +0.6 1,508,782 + Psest_1401 0.25 +0.2 1,508,782 + Psest_1401 0.25 +0.0 1,508,782 + Psest_1401 0.25 +0.9 1,508,782 + Psest_1401 0.25 +0.2 1,508,782 + Psest_1401 0.25 -0.5 1,508,782 + Psest_1401 0.25 -0.1 1,508,782 + Psest_1401 0.25 -1.4 1,508,782 + Psest_1401 0.25 -0.4 1,508,782 + Psest_1401 0.25 -2.0 1,508,783 - Psest_1401 0.25 +2.6 1,508,783 - Psest_1401 0.25 -0.3 1,508,783 - Psest_1401 0.25 -1.1 1,508,783 - Psest_1401 0.25 -1.7 1,508,783 - Psest_1401 0.25 -1.2 1,508,783 - Psest_1401 0.25 -0.6 1,508,783 - Psest_1401 0.25 -1.2 1,508,783 - Psest_1401 0.25 -1.0 1,508,783 - Psest_1401 0.25 -1.2 1,508,783 - Psest_1401 0.25 -1.4 1,508,783 - Psest_1401 0.25 -2.1 1,508,783 - Psest_1401 0.25 +0.7 1,508,783 - Psest_1401 0.25 -1.3 1,509,184 + Psest_1401 0.66 +0.0 1,509,184 + Psest_1401 0.66 +0.1 1,509,184 + Psest_1401 0.66 +0.1 1,509,184 + Psest_1401 0.66 -1.1 1,509,185 - Psest_1401 0.66 -3.5 1,509,185 - Psest_1401 0.66 -1.1 1,509,185 - Psest_1401 0.66 -2.4 1,509,185 - Psest_1401 0.66 +0.9 1,509,185 - Psest_1401 0.66 -0.3 1,509,185 - Psest_1401 0.66 -2.3 1,509,185 - Psest_1401 0.66 -0.4 1,509,185 - Psest_1401 0.66 -1.6 1,509,185 - Psest_1401 0.66 -0.8 1,509,185 - Psest_1401 0.66 -3.2 1,509,247 + Psest_1401 0.72 +2.8 1,509,569 + +0.7 1,509,569 + +0.6 1,509,569 + -1.4 1,509,642 - +0.3 1,509,642 - -1.0 1,509,734 + -1.2 1,509,735 - +0.6 1,509,735 - -0.8 1,510,004 + Psest_1402 0.30 +0.0 1,510,004 + Psest_1402 0.30 -0.3 1,510,004 + Psest_1402 0.30 -0.4 1,510,004 + Psest_1402 0.30 -0.7 1,510,005 - Psest_1402 0.30 +0.1 1,510,005 - Psest_1402 0.30 +1.6 1,510,005 - Psest_1402 0.30 +0.7 1,510,005 - Psest_1402 0.30 -0.2 1,510,035 + Psest_1402 0.35 +0.3 1,510,035 + Psest_1402 0.35 -2.3 1,510,035 + Psest_1402 0.35 -0.1 1,510,035 + Psest_1402 0.35 +0.3 1,510,035 + Psest_1402 0.35 +0.0 1,510,036 - Psest_1402 0.35 +0.4 1,510,036 - Psest_1402 0.35 -1.0 1,510,036 - Psest_1402 0.35 +0.5 1,510,036 - Psest_1402 0.35 +0.9 1,510,036 - Psest_1402 0.35 +0.2 1,510,036 - Psest_1402 0.35 -0.7 1,510,036 - Psest_1402 0.35 -1.0 1,510,103 + Psest_1402 0.46 -1.0 1,510,103 + Psest_1402 0.46 -0.4 1,510,103 + Psest_1402 0.46 +2.3 1,510,103 + Psest_1402 0.46 -0.5 1,510,103 + Psest_1402 0.46 -0.5 1,510,104 - Psest_1402 0.46 +2.2 1,510,104 - Psest_1402 0.46 -1.3 1,510,104 - Psest_1402 0.46 +0.7 1,510,104 - Psest_1402 0.46 -1.4 1,510,130 + Psest_1402 0.50 -1.1 1,510,131 - Psest_1402 0.50 -1.1 1,510,131 - Psest_1402 0.50 -1.4 1,510,131 - Psest_1402 0.50 +0.1 1,510,183 + Psest_1402 0.59 +1.4 1,510,184 - Psest_1402 0.59 +1.6 1,510,184 - Psest_1402 0.59 -0.6 1,510,184 - Psest_1402 0.59 +0.0 1,510,185 + Psest_1402 0.59 -1.7 1,510,185 + Psest_1402 0.59 -1.5 1,510,185 + Psest_1402 0.59 +0.1 1,510,186 - Psest_1402 0.59 -0.9 1,510,436 + -0.7 1,510,436 + +0.4 1,510,436 + -0.5 1,510,436 + +0.6 1,510,436 + +0.9 1,510,437 - +0.4 1,510,437 - +0.6 1,510,437 - -0.7 1,510,437 - -0.8 1,510,437 - +0.0
Or see this region's nucleotide sequence