Experiment: Def Cephalothin 0.08 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1212 and Psest_1213 are separated by 137 nucleotides Psest_1213 and Psest_1214 are separated by 202 nucleotides
Psest_1212: Psest_1212 - Isopenicillin N synthase and related dioxygenases, at 1,317,418 to 1,318,392
_1212
Psest_1213: Psest_1213 - Uncharacterized ABC-type transport system, periplasmic component/surface lipoprotein, at 1,318,530 to 1,319,621
_1213
Psest_1214: Psest_1214 - Cytosine deaminase and related metal-dependent hydrolases, at 1,319,824 to 1,321,179
_1214
Position (kb)
1318
1319
1320 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1317.530 kb on + strand, within Psest_1212 at 1317.531 kb on - strand, within Psest_1212 at 1317.531 kb on - strand, within Psest_1212 at 1317.689 kb on + strand, within Psest_1212 at 1317.689 kb on + strand, within Psest_1212 at 1317.689 kb on + strand, within Psest_1212 at 1317.689 kb on + strand, within Psest_1212 at 1317.689 kb on + strand, within Psest_1212 at 1317.689 kb on + strand, within Psest_1212 at 1317.689 kb on + strand, within Psest_1212 at 1317.689 kb on + strand, within Psest_1212 at 1317.689 kb on + strand, within Psest_1212 at 1317.689 kb on + strand, within Psest_1212 at 1317.689 kb on + strand, within Psest_1212 at 1317.689 kb on + strand, within Psest_1212 at 1317.689 kb on + strand, within Psest_1212 at 1317.689 kb on + strand, within Psest_1212 at 1317.689 kb on + strand, within Psest_1212 at 1317.689 kb on + strand, within Psest_1212 at 1317.689 kb on + strand, within Psest_1212 at 1317.689 kb on + strand, within Psest_1212 at 1317.689 kb on + strand, within Psest_1212 at 1317.690 kb on - strand, within Psest_1212 at 1317.690 kb on - strand, within Psest_1212 at 1317.690 kb on - strand, within Psest_1212 at 1317.690 kb on - strand, within Psest_1212 at 1317.690 kb on - strand, within Psest_1212 at 1317.690 kb on - strand, within Psest_1212 at 1317.690 kb on - strand, within Psest_1212 at 1317.690 kb on - strand, within Psest_1212 at 1317.690 kb on - strand, within Psest_1212 at 1317.690 kb on - strand, within Psest_1212 at 1317.690 kb on - strand, within Psest_1212 at 1317.690 kb on - strand, within Psest_1212 at 1317.690 kb on - strand, within Psest_1212 at 1317.690 kb on - strand, within Psest_1212 at 1317.690 kb on - strand, within Psest_1212 at 1317.690 kb on - strand, within Psest_1212 at 1317.690 kb on - strand, within Psest_1212 at 1317.690 kb on - strand, within Psest_1212 at 1317.690 kb on - strand, within Psest_1212 at 1317.690 kb on - strand, within Psest_1212 at 1317.690 kb on - strand, within Psest_1212 at 1317.690 kb on - strand, within Psest_1212 at 1317.690 kb on - strand, within Psest_1212 at 1317.767 kb on + strand, within Psest_1212 at 1317.768 kb on - strand, within Psest_1212 at 1317.789 kb on - strand, within Psest_1212 at 1318.052 kb on - strand, within Psest_1212 at 1318.074 kb on + strand, within Psest_1212 at 1318.075 kb on - strand, within Psest_1212 at 1318.280 kb on + strand, within Psest_1212 at 1318.280 kb on + strand, within Psest_1212 at 1318.281 kb on - strand, within Psest_1212 at 1318.281 kb on - strand, within Psest_1212 at 1318.281 kb on - strand, within Psest_1212 at 1318.281 kb on - strand, within Psest_1212 at 1318.355 kb on + strand at 1318.355 kb on + strand at 1318.355 kb on + strand at 1318.355 kb on + strand at 1318.355 kb on + strand at 1318.355 kb on + strand at 1318.355 kb on + strand at 1318.356 kb on - strand at 1318.356 kb on - strand at 1318.356 kb on - strand at 1318.356 kb on - strand at 1318.356 kb on - strand at 1318.531 kb on + strand at 1318.531 kb on + strand at 1318.531 kb on + strand at 1318.531 kb on + strand at 1318.564 kb on + strand at 1318.565 kb on - strand at 1318.565 kb on - strand at 1318.565 kb on - strand at 1318.565 kb on - strand at 1318.565 kb on - strand at 1318.567 kb on + strand at 1318.567 kb on + strand at 1318.567 kb on + strand at 1318.567 kb on + strand at 1318.567 kb on + strand at 1318.568 kb on - strand at 1318.568 kb on - strand at 1318.568 kb on - strand at 1318.568 kb on - strand at 1318.568 kb on - strand at 1318.826 kb on - strand, within Psest_1213 at 1318.826 kb on - strand, within Psest_1213 at 1318.910 kb on - strand, within Psest_1213 at 1319.071 kb on + strand, within Psest_1213 at 1319.071 kb on + strand, within Psest_1213 at 1319.071 kb on + strand, within Psest_1213 at 1319.072 kb on - strand, within Psest_1213 at 1319.072 kb on - strand, within Psest_1213 at 1319.072 kb on - strand, within Psest_1213 at 1319.072 kb on - strand, within Psest_1213 at 1319.072 kb on - strand, within Psest_1213 at 1319.072 kb on - strand, within Psest_1213 at 1319.204 kb on - strand, within Psest_1213 at 1319.218 kb on + strand, within Psest_1213 at 1319.219 kb on - strand, within Psest_1213 at 1319.219 kb on - strand, within Psest_1213 at 1319.219 kb on - strand, within Psest_1213 at 1319.219 kb on - strand, within Psest_1213 at 1319.219 kb on - strand, within Psest_1213 at 1319.219 kb on - strand, within Psest_1213 at 1319.311 kb on + strand, within Psest_1213 at 1319.312 kb on - strand, within Psest_1213 at 1319.312 kb on - strand, within Psest_1213 at 1319.312 kb on - strand, within Psest_1213 at 1319.342 kb on - strand, within Psest_1213 at 1319.446 kb on + strand, within Psest_1213 at 1319.446 kb on + strand, within Psest_1213 at 1319.446 kb on + strand, within Psest_1213 at 1319.446 kb on + strand, within Psest_1213 at 1319.447 kb on - strand, within Psest_1213 at 1319.447 kb on - strand, within Psest_1213 at 1319.447 kb on - strand, within Psest_1213 at 1319.447 kb on - strand, within Psest_1213 at 1319.447 kb on - strand, within Psest_1213 at 1319.447 kb on - strand, within Psest_1213 at 1319.503 kb on + strand, within Psest_1213 at 1319.503 kb on + strand, within Psest_1213 at 1319.503 kb on + strand, within Psest_1213 at 1319.503 kb on + strand, within Psest_1213 at 1319.504 kb on - strand, within Psest_1213 at 1319.504 kb on - strand, within Psest_1213 at 1319.504 kb on - strand, within Psest_1213 at 1319.645 kb on + strand at 1319.645 kb on + strand at 1319.646 kb on - strand at 1319.650 kb on - strand at 1319.650 kb on - strand at 1319.670 kb on + strand at 1319.670 kb on + strand at 1319.671 kb on - strand at 1319.671 kb on - strand at 1319.705 kb on + strand at 1319.706 kb on - strand at 1319.706 kb on - strand at 1319.706 kb on - strand at 1320.261 kb on + strand, within Psest_1214 at 1320.261 kb on + strand, within Psest_1214 at 1320.413 kb on + strand, within Psest_1214 at 1320.413 kb on + strand, within Psest_1214 at 1320.483 kb on + strand, within Psest_1214 at 1320.484 kb on - strand, within Psest_1214 at 1320.493 kb on + strand, within Psest_1214 at 1320.493 kb on + strand, within Psest_1214 at 1320.526 kb on - strand, within Psest_1214 at 1320.526 kb on - strand, within Psest_1214 at 1320.526 kb on - strand, within Psest_1214 at 1320.526 kb on - strand, within Psest_1214 at 1320.526 kb on - strand, within Psest_1214
Per-strain Table
Position Strand Gene LocusTag Fraction Def Cephalothin 0.08 mg/ml remove 1,317,530 + Psest_1212 0.11 -1.1 1,317,531 - Psest_1212 0.12 -0.3 1,317,531 - Psest_1212 0.12 -2.4 1,317,689 + Psest_1212 0.28 -1.6 1,317,689 + Psest_1212 0.28 +0.8 1,317,689 + Psest_1212 0.28 -0.7 1,317,689 + Psest_1212 0.28 +0.5 1,317,689 + Psest_1212 0.28 +0.0 1,317,689 + Psest_1212 0.28 -1.7 1,317,689 + Psest_1212 0.28 -1.4 1,317,689 + Psest_1212 0.28 +0.3 1,317,689 + Psest_1212 0.28 +0.4 1,317,689 + Psest_1212 0.28 +0.7 1,317,689 + Psest_1212 0.28 +1.6 1,317,689 + Psest_1212 0.28 -0.2 1,317,689 + Psest_1212 0.28 -2.1 1,317,689 + Psest_1212 0.28 -0.4 1,317,689 + Psest_1212 0.28 -0.7 1,317,689 + Psest_1212 0.28 +2.2 1,317,689 + Psest_1212 0.28 +0.4 1,317,689 + Psest_1212 0.28 -0.2 1,317,689 + Psest_1212 0.28 -0.4 1,317,690 - Psest_1212 0.28 -1.7 1,317,690 - Psest_1212 0.28 -2.3 1,317,690 - Psest_1212 0.28 +0.6 1,317,690 - Psest_1212 0.28 -0.7 1,317,690 - Psest_1212 0.28 +0.4 1,317,690 - Psest_1212 0.28 +0.5 1,317,690 - Psest_1212 0.28 -0.4 1,317,690 - Psest_1212 0.28 +1.9 1,317,690 - Psest_1212 0.28 -2.1 1,317,690 - Psest_1212 0.28 -0.4 1,317,690 - Psest_1212 0.28 +0.3 1,317,690 - Psest_1212 0.28 +0.3 1,317,690 - Psest_1212 0.28 +0.9 1,317,690 - Psest_1212 0.28 -0.3 1,317,690 - Psest_1212 0.28 -2.4 1,317,690 - Psest_1212 0.28 +0.5 1,317,690 - Psest_1212 0.28 +2.0 1,317,690 - Psest_1212 0.28 -0.4 1,317,690 - Psest_1212 0.28 -1.7 1,317,690 - Psest_1212 0.28 +0.0 1,317,690 - Psest_1212 0.28 -0.7 1,317,690 - Psest_1212 0.28 -1.4 1,317,690 - Psest_1212 0.28 -0.5 1,317,767 + Psest_1212 0.36 +1.6 1,317,768 - Psest_1212 0.36 +0.4 1,317,789 - Psest_1212 0.38 +0.0 1,318,052 - Psest_1212 0.65 -0.7 1,318,074 + Psest_1212 0.67 +0.2 1,318,075 - Psest_1212 0.67 +0.3 1,318,280 + Psest_1212 0.88 +0.0 1,318,280 + Psest_1212 0.88 +0.9 1,318,281 - Psest_1212 0.89 +1.3 1,318,281 - Psest_1212 0.89 -0.9 1,318,281 - Psest_1212 0.89 +1.3 1,318,281 - Psest_1212 0.89 -0.7 1,318,355 + -0.2 1,318,355 + +1.1 1,318,355 + +0.0 1,318,355 + +0.3 1,318,355 + -0.4 1,318,355 + -0.7 1,318,355 + -1.5 1,318,356 - -0.5 1,318,356 - +0.2 1,318,356 - +0.4 1,318,356 - -1.1 1,318,356 - -0.9 1,318,531 + -0.1 1,318,531 + +0.0 1,318,531 + +0.6 1,318,531 + +1.0 1,318,564 + +0.9 1,318,565 - -0.7 1,318,565 - -1.1 1,318,565 - -1.0 1,318,565 - -0.9 1,318,565 - -0.3 1,318,567 + -0.5 1,318,567 + +0.0 1,318,567 + -0.7 1,318,567 + -1.4 1,318,567 + +0.5 1,318,568 - -2.3 1,318,568 - +0.0 1,318,568 - +3.2 1,318,568 - -1.2 1,318,568 - -0.2 1,318,826 - Psest_1213 0.27 +1.6 1,318,826 - Psest_1213 0.27 +1.0 1,318,910 - Psest_1213 0.35 -1.6 1,319,071 + Psest_1213 0.50 -1.2 1,319,071 + Psest_1213 0.50 -3.0 1,319,071 + Psest_1213 0.50 -0.4 1,319,072 - Psest_1213 0.50 +0.5 1,319,072 - Psest_1213 0.50 -1.0 1,319,072 - Psest_1213 0.50 -1.7 1,319,072 - Psest_1213 0.50 +0.4 1,319,072 - Psest_1213 0.50 -0.3 1,319,072 - Psest_1213 0.50 -1.2 1,319,204 - Psest_1213 0.62 -1.6 1,319,218 + Psest_1213 0.63 -1.5 1,319,219 - Psest_1213 0.63 -0.4 1,319,219 - Psest_1213 0.63 +1.6 1,319,219 - Psest_1213 0.63 +0.4 1,319,219 - Psest_1213 0.63 +0.0 1,319,219 - Psest_1213 0.63 -2.2 1,319,219 - Psest_1213 0.63 +0.1 1,319,311 + Psest_1213 0.72 +0.6 1,319,312 - Psest_1213 0.72 +1.0 1,319,312 - Psest_1213 0.72 +1.3 1,319,312 - Psest_1213 0.72 +1.0 1,319,342 - Psest_1213 0.74 +1.1 1,319,446 + Psest_1213 0.84 +0.6 1,319,446 + Psest_1213 0.84 -1.2 1,319,446 + Psest_1213 0.84 +0.0 1,319,446 + Psest_1213 0.84 -1.9 1,319,447 - Psest_1213 0.84 -0.4 1,319,447 - Psest_1213 0.84 +0.6 1,319,447 - Psest_1213 0.84 -1.0 1,319,447 - Psest_1213 0.84 -0.3 1,319,447 - Psest_1213 0.84 +1.6 1,319,447 - Psest_1213 0.84 -0.6 1,319,503 + Psest_1213 0.89 +0.3 1,319,503 + Psest_1213 0.89 -1.0 1,319,503 + Psest_1213 0.89 -1.7 1,319,503 + Psest_1213 0.89 +0.6 1,319,504 - Psest_1213 0.89 -0.4 1,319,504 - Psest_1213 0.89 +0.2 1,319,504 - Psest_1213 0.89 -0.4 1,319,645 + -1.6 1,319,645 + -0.3 1,319,646 - +0.6 1,319,650 - +0.1 1,319,650 - -1.7 1,319,670 + -0.7 1,319,670 + +2.2 1,319,671 - +0.7 1,319,671 - +0.0 1,319,705 + -0.1 1,319,706 - -0.9 1,319,706 - -1.4 1,319,706 - +0.2 1,320,261 + Psest_1214 0.32 +0.1 1,320,261 + Psest_1214 0.32 -1.3 1,320,413 + Psest_1214 0.43 -0.4 1,320,413 + Psest_1214 0.43 +0.3 1,320,483 + Psest_1214 0.49 -0.5 1,320,484 - Psest_1214 0.49 -0.7 1,320,493 + Psest_1214 0.49 +0.4 1,320,493 + Psest_1214 0.49 -1.2 1,320,526 - Psest_1214 0.52 -0.6 1,320,526 - Psest_1214 0.52 +0.0 1,320,526 - Psest_1214 0.52 +1.9 1,320,526 - Psest_1214 0.52 -1.4 1,320,526 - Psest_1214 0.52 -2.1
Or see this region's nucleotide sequence