Experiment: Def Cephalothin 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0890 and Psest_0891 are separated by 59 nucleotides Psest_0891 and Psest_0892 are separated by 60 nucleotides Psest_0892 and Psest_0893 are separated by 108 nucleotides
Psest_0890: Psest_0890 - S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase, at 966,921 to 968,030
_0890
Psest_0891: Psest_0891 - Uncharacterized protein conserved in bacteria, at 968,090 to 968,314
_0891
Psest_0892: Psest_0892 - Short-chain alcohol dehydrogenase of unknown specificity, at 968,375 to 969,154
_0892
Psest_0893: Psest_0893 - Transcriptional regulator, at 969,263 to 970,177
_0893
Position (kb)
968
969
970 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 967.600 kb on + strand, within Psest_0890 at 967.601 kb on - strand, within Psest_0890 at 967.627 kb on + strand, within Psest_0890 at 967.627 kb on + strand, within Psest_0890 at 967.627 kb on + strand, within Psest_0890 at 967.627 kb on + strand, within Psest_0890 at 967.628 kb on - strand, within Psest_0890 at 967.628 kb on - strand, within Psest_0890 at 967.628 kb on - strand, within Psest_0890 at 967.628 kb on - strand, within Psest_0890 at 967.628 kb on - strand, within Psest_0890 at 967.633 kb on + strand, within Psest_0890 at 967.634 kb on - strand, within Psest_0890 at 967.634 kb on - strand, within Psest_0890 at 967.634 kb on - strand, within Psest_0890 at 967.634 kb on - strand, within Psest_0890 at 967.651 kb on + strand, within Psest_0890 at 967.651 kb on + strand, within Psest_0890 at 967.651 kb on + strand, within Psest_0890 at 967.651 kb on + strand, within Psest_0890 at 967.651 kb on + strand, within Psest_0890 at 967.652 kb on - strand, within Psest_0890 at 967.768 kb on + strand, within Psest_0890 at 967.769 kb on - strand, within Psest_0890 at 968.045 kb on + strand at 968.045 kb on + strand at 968.046 kb on - strand at 968.048 kb on + strand at 968.048 kb on + strand at 968.048 kb on + strand at 968.049 kb on - strand at 968.049 kb on - strand at 968.049 kb on - strand at 968.049 kb on - strand at 968.049 kb on - strand at 968.049 kb on - strand at 968.049 kb on - strand at 968.052 kb on + strand at 968.053 kb on - strand at 968.053 kb on - strand at 968.053 kb on - strand at 968.057 kb on - strand at 968.057 kb on - strand at 968.092 kb on - strand at 968.101 kb on - strand at 968.101 kb on - strand at 968.101 kb on - strand at 968.187 kb on + strand, within Psest_0891 at 968.187 kb on + strand, within Psest_0891 at 968.187 kb on + strand, within Psest_0891 at 968.187 kb on + strand, within Psest_0891 at 968.187 kb on + strand, within Psest_0891 at 968.187 kb on + strand, within Psest_0891 at 968.188 kb on - strand, within Psest_0891 at 968.188 kb on - strand, within Psest_0891 at 968.188 kb on - strand, within Psest_0891 at 968.188 kb on - strand, within Psest_0891 at 968.188 kb on - strand, within Psest_0891 at 968.188 kb on - strand, within Psest_0891 at 968.188 kb on - strand, within Psest_0891 at 968.188 kb on - strand, within Psest_0891 at 968.188 kb on - strand, within Psest_0891 at 968.335 kb on + strand at 968.377 kb on - strand at 968.413 kb on + strand at 968.490 kb on + strand, within Psest_0892 at 968.490 kb on + strand, within Psest_0892 at 968.490 kb on + strand, within Psest_0892 at 968.490 kb on + strand, within Psest_0892 at 968.490 kb on + strand, within Psest_0892 at 968.491 kb on - strand, within Psest_0892 at 968.491 kb on - strand, within Psest_0892 at 968.491 kb on - strand, within Psest_0892 at 968.491 kb on - strand, within Psest_0892 at 968.491 kb on - strand, within Psest_0892 at 968.491 kb on - strand, within Psest_0892 at 968.620 kb on + strand, within Psest_0892 at 968.621 kb on - strand, within Psest_0892 at 968.649 kb on + strand, within Psest_0892 at 968.758 kb on - strand, within Psest_0892 at 968.780 kb on - strand, within Psest_0892 at 968.858 kb on + strand, within Psest_0892 at 968.859 kb on - strand, within Psest_0892 at 968.859 kb on - strand, within Psest_0892 at 968.859 kb on - strand, within Psest_0892 at 968.859 kb on - strand, within Psest_0892 at 968.859 kb on - strand, within Psest_0892 at 968.859 kb on - strand, within Psest_0892 at 968.859 kb on - strand, within Psest_0892 at 968.859 kb on - strand, within Psest_0892 at 968.859 kb on - strand, within Psest_0892 at 968.859 kb on - strand, within Psest_0892 at 968.874 kb on + strand, within Psest_0892 at 968.874 kb on + strand, within Psest_0892 at 968.875 kb on - strand, within Psest_0892 at 968.923 kb on + strand, within Psest_0892 at 968.923 kb on + strand, within Psest_0892 at 968.923 kb on + strand, within Psest_0892 at 968.924 kb on - strand, within Psest_0892 at 968.924 kb on - strand, within Psest_0892 at 968.924 kb on - strand, within Psest_0892 at 968.992 kb on + strand, within Psest_0892 at 968.993 kb on - strand, within Psest_0892 at 969.018 kb on - strand, within Psest_0892 at 969.145 kb on - strand at 969.184 kb on + strand at 969.185 kb on - strand at 969.185 kb on - strand at 969.185 kb on - strand at 969.206 kb on - strand at 969.230 kb on - strand at 969.269 kb on - strand at 969.287 kb on - strand at 969.316 kb on + strand at 969.316 kb on + strand at 969.316 kb on + strand at 969.317 kb on - strand at 969.317 kb on - strand at 969.458 kb on + strand, within Psest_0893 at 969.538 kb on + strand, within Psest_0893 at 969.539 kb on - strand, within Psest_0893 at 969.582 kb on + strand, within Psest_0893 at 969.683 kb on - strand, within Psest_0893 at 969.719 kb on + strand, within Psest_0893 at 969.719 kb on + strand, within Psest_0893 at 969.735 kb on + strand, within Psest_0893 at 969.736 kb on - strand, within Psest_0893 at 969.753 kb on - strand, within Psest_0893 at 969.753 kb on - strand, within Psest_0893 at 969.796 kb on + strand, within Psest_0893 at 969.796 kb on + strand, within Psest_0893 at 969.796 kb on + strand, within Psest_0893 at 969.797 kb on - strand, within Psest_0893 at 969.797 kb on - strand, within Psest_0893 at 969.813 kb on + strand, within Psest_0893 at 969.817 kb on + strand, within Psest_0893 at 969.817 kb on + strand, within Psest_0893 at 969.817 kb on + strand, within Psest_0893 at 969.817 kb on + strand, within Psest_0893 at 969.817 kb on + strand, within Psest_0893 at 969.817 kb on + strand, within Psest_0893 at 969.817 kb on + strand, within Psest_0893 at 969.817 kb on + strand, within Psest_0893 at 969.818 kb on - strand, within Psest_0893 at 969.818 kb on - strand, within Psest_0893 at 969.873 kb on + strand, within Psest_0893 at 969.874 kb on - strand, within Psest_0893 at 969.874 kb on - strand, within Psest_0893 at 969.916 kb on + strand, within Psest_0893 at 969.916 kb on + strand, within Psest_0893 at 969.916 kb on + strand, within Psest_0893 at 970.068 kb on + strand, within Psest_0893 at 970.068 kb on + strand, within Psest_0893 at 970.068 kb on + strand, within Psest_0893 at 970.068 kb on + strand, within Psest_0893 at 970.068 kb on + strand, within Psest_0893 at 970.068 kb on + strand, within Psest_0893 at 970.069 kb on - strand, within Psest_0893 at 970.069 kb on - strand, within Psest_0893 at 970.069 kb on - strand, within Psest_0893 at 970.070 kb on + strand, within Psest_0893 at 970.070 kb on + strand, within Psest_0893 at 970.070 kb on + strand, within Psest_0893 at 970.070 kb on + strand, within Psest_0893 at 970.070 kb on + strand, within Psest_0893 at 970.070 kb on + strand, within Psest_0893 at 970.070 kb on + strand, within Psest_0893 at 970.070 kb on + strand, within Psest_0893 at 970.071 kb on - strand, within Psest_0893 at 970.071 kb on - strand, within Psest_0893 at 970.071 kb on - strand, within Psest_0893 at 970.116 kb on - strand at 970.116 kb on - strand at 970.126 kb on + strand at 970.127 kb on - strand at 970.127 kb on - strand at 970.127 kb on - strand at 970.152 kb on + strand at 970.152 kb on + strand at 970.152 kb on + strand at 970.153 kb on - strand at 970.153 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Cephalothin 0.04 mg/ml remove 967,600 + Psest_0890 0.61 +1.0 967,601 - Psest_0890 0.61 -0.4 967,627 + Psest_0890 0.64 -2.4 967,627 + Psest_0890 0.64 -0.2 967,627 + Psest_0890 0.64 +0.8 967,627 + Psest_0890 0.64 -0.4 967,628 - Psest_0890 0.64 +0.8 967,628 - Psest_0890 0.64 -0.0 967,628 - Psest_0890 0.64 +0.1 967,628 - Psest_0890 0.64 +1.1 967,628 - Psest_0890 0.64 +0.4 967,633 + Psest_0890 0.64 -0.2 967,634 - Psest_0890 0.64 -0.6 967,634 - Psest_0890 0.64 +0.2 967,634 - Psest_0890 0.64 +1.0 967,634 - Psest_0890 0.64 -0.2 967,651 + Psest_0890 0.66 +0.1 967,651 + Psest_0890 0.66 -1.5 967,651 + Psest_0890 0.66 -0.8 967,651 + Psest_0890 0.66 +0.6 967,651 + Psest_0890 0.66 -2.2 967,652 - Psest_0890 0.66 +0.2 967,768 + Psest_0890 0.76 +0.1 967,769 - Psest_0890 0.76 -0.1 968,045 + +0.4 968,045 + +0.5 968,046 - +0.1 968,048 + -0.4 968,048 + +0.6 968,048 + -0.1 968,049 - -1.0 968,049 - -0.9 968,049 - -3.2 968,049 - -0.2 968,049 - -0.2 968,049 - -1.4 968,049 - -0.7 968,052 + +1.2 968,053 - -1.4 968,053 - -1.7 968,053 - -0.8 968,057 - +0.3 968,057 - -1.4 968,092 - -2.8 968,101 - -0.1 968,101 - -0.3 968,101 - -1.4 968,187 + Psest_0891 0.43 -0.7 968,187 + Psest_0891 0.43 +1.1 968,187 + Psest_0891 0.43 +0.2 968,187 + Psest_0891 0.43 +0.2 968,187 + Psest_0891 0.43 +0.5 968,187 + Psest_0891 0.43 -1.7 968,188 - Psest_0891 0.44 -1.5 968,188 - Psest_0891 0.44 +0.4 968,188 - Psest_0891 0.44 +0.0 968,188 - Psest_0891 0.44 -0.5 968,188 - Psest_0891 0.44 -2.5 968,188 - Psest_0891 0.44 +0.4 968,188 - Psest_0891 0.44 +0.5 968,188 - Psest_0891 0.44 -0.1 968,188 - Psest_0891 0.44 -0.1 968,335 + +1.0 968,377 - -0.3 968,413 + +0.3 968,490 + Psest_0892 0.15 +0.2 968,490 + Psest_0892 0.15 -1.1 968,490 + Psest_0892 0.15 -0.3 968,490 + Psest_0892 0.15 +0.9 968,490 + Psest_0892 0.15 -0.5 968,491 - Psest_0892 0.15 +0.9 968,491 - Psest_0892 0.15 -0.6 968,491 - Psest_0892 0.15 -2.8 968,491 - Psest_0892 0.15 -0.7 968,491 - Psest_0892 0.15 -0.5 968,491 - Psest_0892 0.15 +0.6 968,620 + Psest_0892 0.31 -0.6 968,621 - Psest_0892 0.32 -1.8 968,649 + Psest_0892 0.35 -0.6 968,758 - Psest_0892 0.49 +0.2 968,780 - Psest_0892 0.52 -0.0 968,858 + Psest_0892 0.62 -0.0 968,859 - Psest_0892 0.62 +0.0 968,859 - Psest_0892 0.62 +1.0 968,859 - Psest_0892 0.62 -0.6 968,859 - Psest_0892 0.62 -1.6 968,859 - Psest_0892 0.62 +0.3 968,859 - Psest_0892 0.62 -0.6 968,859 - Psest_0892 0.62 -2.8 968,859 - Psest_0892 0.62 -1.9 968,859 - Psest_0892 0.62 +0.8 968,859 - Psest_0892 0.62 -0.3 968,874 + Psest_0892 0.64 +0.4 968,874 + Psest_0892 0.64 -0.0 968,875 - Psest_0892 0.64 -2.1 968,923 + Psest_0892 0.70 +1.5 968,923 + Psest_0892 0.70 -0.2 968,923 + Psest_0892 0.70 -0.8 968,924 - Psest_0892 0.70 -1.1 968,924 - Psest_0892 0.70 -1.3 968,924 - Psest_0892 0.70 -2.8 968,992 + Psest_0892 0.79 -2.1 968,993 - Psest_0892 0.79 -0.6 969,018 - Psest_0892 0.82 -0.4 969,145 - -1.6 969,184 + +0.1 969,185 - -0.1 969,185 - -1.5 969,185 - +0.5 969,206 - +0.5 969,230 - -1.3 969,269 - +0.6 969,287 - +0.5 969,316 + +0.8 969,316 + -0.4 969,316 + -0.1 969,317 - -1.6 969,317 - +0.5 969,458 + Psest_0893 0.21 +1.6 969,538 + Psest_0893 0.30 -2.3 969,539 - Psest_0893 0.30 +1.6 969,582 + Psest_0893 0.35 +0.4 969,683 - Psest_0893 0.46 +0.1 969,719 + Psest_0893 0.50 +0.9 969,719 + Psest_0893 0.50 +0.9 969,735 + Psest_0893 0.52 -1.3 969,736 - Psest_0893 0.52 +0.5 969,753 - Psest_0893 0.54 +0.9 969,753 - Psest_0893 0.54 -2.4 969,796 + Psest_0893 0.58 +0.0 969,796 + Psest_0893 0.58 -2.4 969,796 + Psest_0893 0.58 +0.1 969,797 - Psest_0893 0.58 -0.5 969,797 - Psest_0893 0.58 -0.7 969,813 + Psest_0893 0.60 -1.5 969,817 + Psest_0893 0.61 -1.1 969,817 + Psest_0893 0.61 +0.5 969,817 + Psest_0893 0.61 +0.1 969,817 + Psest_0893 0.61 -0.6 969,817 + Psest_0893 0.61 +1.0 969,817 + Psest_0893 0.61 -2.6 969,817 + Psest_0893 0.61 +0.1 969,817 + Psest_0893 0.61 -0.7 969,818 - Psest_0893 0.61 -2.6 969,818 - Psest_0893 0.61 -0.6 969,873 + Psest_0893 0.67 -0.6 969,874 - Psest_0893 0.67 -0.0 969,874 - Psest_0893 0.67 +1.8 969,916 + Psest_0893 0.71 -0.3 969,916 + Psest_0893 0.71 -1.2 969,916 + Psest_0893 0.71 -0.8 970,068 + Psest_0893 0.88 -0.5 970,068 + Psest_0893 0.88 -0.7 970,068 + Psest_0893 0.88 +0.9 970,068 + Psest_0893 0.88 -0.6 970,068 + Psest_0893 0.88 -0.1 970,068 + Psest_0893 0.88 +0.2 970,069 - Psest_0893 0.88 +1.4 970,069 - Psest_0893 0.88 -1.3 970,069 - Psest_0893 0.88 -0.2 970,070 + Psest_0893 0.88 +1.1 970,070 + Psest_0893 0.88 -0.6 970,070 + Psest_0893 0.88 -0.3 970,070 + Psest_0893 0.88 -0.2 970,070 + Psest_0893 0.88 +0.1 970,070 + Psest_0893 0.88 +0.5 970,070 + Psest_0893 0.88 -0.1 970,070 + Psest_0893 0.88 -0.0 970,071 - Psest_0893 0.88 +2.4 970,071 - Psest_0893 0.88 +2.3 970,071 - Psest_0893 0.88 -1.8 970,116 - +0.0 970,116 - -0.5 970,126 + -1.3 970,127 - -2.4 970,127 - +0.1 970,127 - -0.6 970,152 + -0.4 970,152 + +0.0 970,152 + -0.8 970,153 - -0.7 970,153 - -0.4
Or see this region's nucleotide sequence