Experiment: Def Cephalothin 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3674 and Psest_3675 are separated by 95 nucleotides Psest_3675 and Psest_3676 are separated by 6 nucleotides Psest_3676 and Psest_3677 overlap by 10 nucleotides Psest_3677 and Psest_3678 are separated by 75 nucleotides
Psest_3674: Psest_3674 - Uncharacterized conserved protein, at 3,838,535 to 3,840,082
_3674
Psest_3675: Psest_3675 - tRNA nucleotidyltransferase/poly(A) polymerase, at 3,840,178 to 3,841,347
_3675
Psest_3676: Psest_3676 - 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase, at 3,841,354 to 3,841,896
_3676
Psest_3677: Psest_3677 - dihydroneopterin aldolase, at 3,841,887 to 3,842,240
_3677
Psest_3678: Psest_3678 - acyl-phosphate glycerol 3-phosphate acyltransferase, at 3,842,316 to 3,842,885
_3678
Position (kb)
3840
3841
3842 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3839.246 kb on + strand, within Psest_3674 at 3839.246 kb on + strand, within Psest_3674 at 3839.246 kb on + strand, within Psest_3674 at 3839.247 kb on - strand, within Psest_3674 at 3839.247 kb on - strand, within Psest_3674 at 3839.324 kb on + strand, within Psest_3674 at 3839.324 kb on + strand, within Psest_3674 at 3839.324 kb on + strand, within Psest_3674 at 3839.325 kb on - strand, within Psest_3674 at 3839.325 kb on - strand, within Psest_3674 at 3839.325 kb on - strand, within Psest_3674 at 3839.330 kb on + strand, within Psest_3674 at 3839.385 kb on - strand, within Psest_3674 at 3839.385 kb on - strand, within Psest_3674 at 3839.385 kb on - strand, within Psest_3674 at 3839.385 kb on - strand, within Psest_3674 at 3839.385 kb on - strand, within Psest_3674 at 3839.405 kb on + strand, within Psest_3674 at 3839.406 kb on - strand, within Psest_3674 at 3839.471 kb on + strand, within Psest_3674 at 3839.496 kb on - strand, within Psest_3674 at 3839.498 kb on + strand, within Psest_3674 at 3839.498 kb on + strand, within Psest_3674 at 3839.498 kb on + strand, within Psest_3674 at 3839.498 kb on + strand, within Psest_3674 at 3839.498 kb on + strand, within Psest_3674 at 3839.499 kb on - strand, within Psest_3674 at 3839.499 kb on - strand, within Psest_3674 at 3839.499 kb on - strand, within Psest_3674 at 3839.516 kb on + strand, within Psest_3674 at 3839.517 kb on - strand, within Psest_3674 at 3839.537 kb on + strand, within Psest_3674 at 3839.537 kb on + strand, within Psest_3674 at 3839.537 kb on + strand, within Psest_3674 at 3839.538 kb on - strand, within Psest_3674 at 3839.538 kb on - strand, within Psest_3674 at 3839.538 kb on - strand, within Psest_3674 at 3839.538 kb on - strand, within Psest_3674 at 3839.538 kb on - strand, within Psest_3674 at 3839.538 kb on - strand, within Psest_3674 at 3839.538 kb on - strand, within Psest_3674 at 3839.810 kb on + strand, within Psest_3674 at 3839.810 kb on + strand, within Psest_3674 at 3839.810 kb on + strand, within Psest_3674 at 3839.810 kb on + strand, within Psest_3674 at 3839.810 kb on + strand, within Psest_3674 at 3839.811 kb on - strand, within Psest_3674 at 3839.811 kb on - strand, within Psest_3674 at 3839.811 kb on - strand, within Psest_3674 at 3839.811 kb on - strand, within Psest_3674 at 3839.811 kb on - strand, within Psest_3674 at 3839.811 kb on - strand, within Psest_3674 at 3839.935 kb on - strand at 3839.935 kb on - strand at 3839.935 kb on - strand at 3840.096 kb on + strand at 3840.097 kb on - strand at 3840.097 kb on - strand at 3840.152 kb on + strand at 3840.177 kb on + strand at 3840.187 kb on + strand at 3840.187 kb on + strand at 3840.187 kb on + strand at 3840.187 kb on + strand at 3840.187 kb on + strand at 3840.187 kb on + strand at 3841.325 kb on - strand at 3841.325 kb on - strand at 3841.325 kb on - strand at 3841.345 kb on + strand at 3841.345 kb on + strand at 3841.345 kb on + strand at 3841.346 kb on - strand at 3841.346 kb on - strand at 3841.346 kb on - strand at 3841.346 kb on - strand at 3841.346 kb on - strand at 3841.465 kb on + strand, within Psest_3676
Per-strain Table
Position Strand Gene LocusTag Fraction Def Cephalothin 0.04 mg/ml remove 3,839,246 + Psest_3674 0.46 +0.4 3,839,246 + Psest_3674 0.46 -0.4 3,839,246 + Psest_3674 0.46 +0.5 3,839,247 - Psest_3674 0.46 +0.9 3,839,247 - Psest_3674 0.46 +0.3 3,839,324 + Psest_3674 0.51 -0.7 3,839,324 + Psest_3674 0.51 -1.2 3,839,324 + Psest_3674 0.51 +0.6 3,839,325 - Psest_3674 0.51 +0.3 3,839,325 - Psest_3674 0.51 -0.1 3,839,325 - Psest_3674 0.51 -2.3 3,839,330 + Psest_3674 0.51 +1.5 3,839,385 - Psest_3674 0.55 +1.1 3,839,385 - Psest_3674 0.55 -1.5 3,839,385 - Psest_3674 0.55 +0.6 3,839,385 - Psest_3674 0.55 -0.8 3,839,385 - Psest_3674 0.55 +0.3 3,839,405 + Psest_3674 0.56 +0.1 3,839,406 - Psest_3674 0.56 +0.4 3,839,471 + Psest_3674 0.60 -1.2 3,839,496 - Psest_3674 0.62 +1.6 3,839,498 + Psest_3674 0.62 -1.7 3,839,498 + Psest_3674 0.62 +0.1 3,839,498 + Psest_3674 0.62 +1.1 3,839,498 + Psest_3674 0.62 +0.1 3,839,498 + Psest_3674 0.62 +1.1 3,839,499 - Psest_3674 0.62 -0.0 3,839,499 - Psest_3674 0.62 -1.0 3,839,499 - Psest_3674 0.62 -0.3 3,839,516 + Psest_3674 0.63 +0.4 3,839,517 - Psest_3674 0.63 -1.0 3,839,537 + Psest_3674 0.65 -1.0 3,839,537 + Psest_3674 0.65 +0.1 3,839,537 + Psest_3674 0.65 +0.6 3,839,538 - Psest_3674 0.65 -0.2 3,839,538 - Psest_3674 0.65 -0.4 3,839,538 - Psest_3674 0.65 +1.1 3,839,538 - Psest_3674 0.65 +0.6 3,839,538 - Psest_3674 0.65 -0.0 3,839,538 - Psest_3674 0.65 +1.4 3,839,538 - Psest_3674 0.65 -1.6 3,839,810 + Psest_3674 0.82 -2.3 3,839,810 + Psest_3674 0.82 +2.4 3,839,810 + Psest_3674 0.82 +0.1 3,839,810 + Psest_3674 0.82 +0.4 3,839,810 + Psest_3674 0.82 -0.4 3,839,811 - Psest_3674 0.82 +0.3 3,839,811 - Psest_3674 0.82 -2.9 3,839,811 - Psest_3674 0.82 -1.2 3,839,811 - Psest_3674 0.82 +0.3 3,839,811 - Psest_3674 0.82 -0.6 3,839,811 - Psest_3674 0.82 -1.1 3,839,935 - +0.9 3,839,935 - -1.4 3,839,935 - -1.4 3,840,096 + +0.6 3,840,097 - -0.2 3,840,097 - -0.9 3,840,152 + -0.6 3,840,177 + -1.4 3,840,187 + +0.4 3,840,187 + -1.4 3,840,187 + -2.8 3,840,187 + +0.3 3,840,187 + -2.8 3,840,187 + -1.6 3,841,325 - -0.3 3,841,325 - +0.1 3,841,325 - -0.5 3,841,345 + +0.4 3,841,345 + +1.4 3,841,345 + +0.3 3,841,346 - -1.2 3,841,346 - -2.4 3,841,346 - -0.2 3,841,346 - +1.9 3,841,346 - -1.7 3,841,465 + Psest_3676 0.20 +1.6
Or see this region's nucleotide sequence