Experiment: Def Cephalothin 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0256 and Psest_0257 overlap by 8 nucleotides Psest_0257 and Psest_0258 are separated by 229 nucleotides
Psest_0256: Psest_0256 - Predicted Zn-dependent peptidases, at 269,463 to 271,004
_0256
Psest_0257: Psest_0257 - Predicted Zn-dependent peptidases, at 270,997 to 272,349
_0257
Psest_0258: Psest_0258 - signal recognition particle-docking protein FtsY, at 272,579 to 273,859
_0258
Position (kb) 270
271
272
273 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 271.024 kb on + strand at 271.024 kb on + strand at 271.024 kb on + strand at 271.024 kb on + strand at 271.024 kb on + strand at 271.025 kb on - strand at 271.025 kb on - strand at 271.025 kb on - strand at 271.025 kb on - strand at 271.025 kb on - strand at 271.025 kb on - strand at 271.025 kb on - strand at 271.025 kb on - strand at 271.058 kb on + strand at 271.059 kb on - strand at 271.276 kb on + strand, within Psest_0257 at 271.731 kb on - strand, within Psest_0257 at 271.850 kb on + strand, within Psest_0257 at 272.181 kb on - strand, within Psest_0257 at 272.350 kb on - strand at 272.350 kb on - strand at 272.459 kb on + strand at 272.512 kb on + strand at 272.512 kb on + strand at 272.512 kb on + strand at 272.517 kb on + strand at 272.552 kb on + strand at 272.569 kb on + strand at 272.569 kb on + strand at 272.569 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Cephalothin 0.04 mg/ml remove 271,024 + -2.9 271,024 + -1.7 271,024 + -1.4 271,024 + +0.0 271,024 + +0.3 271,025 - +0.2 271,025 - +0.8 271,025 - +0.5 271,025 - +1.1 271,025 - -0.1 271,025 - +0.2 271,025 - -0.2 271,025 - +0.4 271,058 + +1.4 271,059 - -1.4 271,276 + Psest_0257 0.21 -1.8 271,731 - Psest_0257 0.54 -1.0 271,850 + Psest_0257 0.63 +0.6 272,181 - Psest_0257 0.88 +0.0 272,350 - -0.6 272,350 - +0.4 272,459 + -2.5 272,512 + -0.7 272,512 + +0.4 272,512 + +0.3 272,517 + +0.5 272,552 + -1.4 272,569 + +0.3 272,569 + +1.4 272,569 + +1.6
Or see this region's nucleotide sequence