Experiment: Def Cephalothin 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2583 and Psest_2584 are separated by 210 nucleotides Psest_2584 and Psest_2585 are separated by 91 nucleotides Psest_2585 and Psest_2586 are separated by 2 nucleotides
Psest_2583: Psest_2583 - acetyl-CoA carboxylase, carboxyl transferase, beta subunit, at 2,763,339 to 2,764,232
_2583
Psest_2584: Psest_2584 - Phosphoribosylanthranilate isomerase, at 2,764,443 to 2,765,066
_2584
Psest_2585: Psest_2585 - pseudouridylate synthase I, at 2,765,158 to 2,766,018
_2585
Psest_2586: Psest_2586 - FimV N-terminal domain, at 2,766,021 to 2,768,753
_2586
Position (kb)
2764
2765
2766 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 2764.305 kb on - strand at 2764.305 kb on - strand at 2764.305 kb on - strand at 2764.390 kb on - strand at 2764.683 kb on - strand, within Psest_2584 at 2764.695 kb on - strand, within Psest_2584 at 2764.720 kb on - strand, within Psest_2584 at 2764.896 kb on - strand, within Psest_2584 at 2764.899 kb on - strand, within Psest_2584 at 2764.977 kb on + strand, within Psest_2584 at 2764.977 kb on + strand, within Psest_2584 at 2764.978 kb on - strand, within Psest_2584 at 2764.978 kb on - strand, within Psest_2584 at 2764.978 kb on - strand, within Psest_2584 at 2765.043 kb on + strand at 2765.044 kb on - strand at 2765.071 kb on + strand at 2765.071 kb on + strand at 2765.075 kb on + strand at 2765.075 kb on + strand at 2765.075 kb on + strand at 2765.075 kb on + strand at 2765.076 kb on - strand at 2765.080 kb on - strand at 2765.476 kb on - strand, within Psest_2585 at 2765.754 kb on + strand, within Psest_2585 at 2765.942 kb on + strand at 2766.041 kb on + strand at 2766.042 kb on - strand at 2766.042 kb on - strand at 2766.042 kb on - strand at 2766.042 kb on - strand at 2766.042 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Cephalothin 0.04 mg/ml remove 2,764,305 - -0.3 2,764,305 - +0.1 2,764,305 - +1.3 2,764,390 - -0.3 2,764,683 - Psest_2584 0.38 -2.4 2,764,695 - Psest_2584 0.40 -4.3 2,764,720 - Psest_2584 0.44 -2.7 2,764,896 - Psest_2584 0.73 -1.8 2,764,899 - Psest_2584 0.73 -4.8 2,764,977 + Psest_2584 0.86 -1.7 2,764,977 + Psest_2584 0.86 -1.8 2,764,978 - Psest_2584 0.86 -2.3 2,764,978 - Psest_2584 0.86 -6.6 2,764,978 - Psest_2584 0.86 -2.0 2,765,043 + -3.2 2,765,044 - -1.9 2,765,071 + -3.3 2,765,071 + -3.4 2,765,075 + -1.2 2,765,075 + -4.8 2,765,075 + -2.7 2,765,075 + -2.8 2,765,076 - -2.7 2,765,080 - -1.1 2,765,476 - Psest_2585 0.37 -1.4 2,765,754 + Psest_2585 0.69 -0.6 2,765,942 + -1.2 2,766,041 + -3.2 2,766,042 - -0.0 2,766,042 - -0.2 2,766,042 - +0.5 2,766,042 - +0.9 2,766,042 - -0.5
Or see this region's nucleotide sequence