Experiment: Def Cephalothin 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1544 and Psest_1545 are separated by 128 nucleotides Psest_1545 and Psest_1546 are separated by 95 nucleotides
Psest_1544: Psest_1544 - single-stranded-DNA-specific exonuclease RecJ, at 1,642,565 to 1,644,280
_1544
Psest_1545: Psest_1545 - Anaerobic dehydrogenases, typically selenocysteine-containing, at 1,644,409 to 1,646,502
_1545
Psest_1546: Psest_1546 - NADH:flavin oxidoreductases, Old Yellow Enzyme family, at 1,646,598 to 1,647,707
_1546
Position (kb)
1644
1645
1646
1647 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1644.054 kb on + strand, within Psest_1544 at 1644.207 kb on - strand at 1644.303 kb on - strand at 1644.369 kb on - strand at 1644.540 kb on + strand at 1644.718 kb on + strand, within Psest_1545 at 1644.745 kb on + strand, within Psest_1545 at 1644.769 kb on + strand, within Psest_1545 at 1644.769 kb on + strand, within Psest_1545 at 1644.769 kb on + strand, within Psest_1545 at 1644.769 kb on + strand, within Psest_1545 at 1644.770 kb on - strand, within Psest_1545 at 1644.770 kb on - strand, within Psest_1545 at 1644.770 kb on - strand, within Psest_1545 at 1644.770 kb on - strand, within Psest_1545 at 1644.856 kb on + strand, within Psest_1545 at 1644.857 kb on - strand, within Psest_1545 at 1644.872 kb on + strand, within Psest_1545 at 1644.873 kb on - strand, within Psest_1545 at 1644.873 kb on - strand, within Psest_1545 at 1645.657 kb on + strand, within Psest_1545 at 1645.657 kb on + strand, within Psest_1545 at 1645.657 kb on + strand, within Psest_1545 at 1645.658 kb on - strand, within Psest_1545 at 1645.658 kb on - strand, within Psest_1545 at 1645.658 kb on - strand, within Psest_1545 at 1645.679 kb on - strand, within Psest_1545 at 1645.679 kb on - strand, within Psest_1545 at 1645.727 kb on - strand, within Psest_1545 at 1645.727 kb on - strand, within Psest_1545 at 1645.759 kb on - strand, within Psest_1545 at 1645.995 kb on - strand, within Psest_1545 at 1646.147 kb on + strand, within Psest_1545 at 1646.147 kb on + strand, within Psest_1545 at 1646.147 kb on + strand, within Psest_1545 at 1646.147 kb on + strand, within Psest_1545 at 1646.147 kb on + strand, within Psest_1545 at 1646.147 kb on + strand, within Psest_1545 at 1646.148 kb on - strand, within Psest_1545 at 1646.176 kb on + strand, within Psest_1545 at 1646.176 kb on + strand, within Psest_1545 at 1646.301 kb on - strand at 1646.301 kb on - strand at 1646.301 kb on - strand at 1646.451 kb on - strand at 1646.588 kb on - strand at 1646.792 kb on + strand, within Psest_1546 at 1646.792 kb on + strand, within Psest_1546 at 1646.792 kb on + strand, within Psest_1546 at 1646.793 kb on - strand, within Psest_1546 at 1646.793 kb on - strand, within Psest_1546 at 1646.913 kb on - strand, within Psest_1546 at 1646.913 kb on - strand, within Psest_1546 at 1646.913 kb on - strand, within Psest_1546 at 1647.124 kb on + strand, within Psest_1546 at 1647.124 kb on + strand, within Psest_1546 at 1647.124 kb on + strand, within Psest_1546 at 1647.125 kb on - strand, within Psest_1546 at 1647.125 kb on - strand, within Psest_1546
Per-strain Table
Position Strand Gene LocusTag Fraction Def Cephalothin 0.04 mg/ml remove 1,644,054 + Psest_1544 0.87 -0.6 1,644,207 - -1.0 1,644,303 - -1.1 1,644,369 - +2.2 1,644,540 + +1.1 1,644,718 + Psest_1545 0.15 -0.6 1,644,745 + Psest_1545 0.16 +0.3 1,644,769 + Psest_1545 0.17 -0.3 1,644,769 + Psest_1545 0.17 -1.5 1,644,769 + Psest_1545 0.17 +0.2 1,644,769 + Psest_1545 0.17 -1.0 1,644,770 - Psest_1545 0.17 +0.0 1,644,770 - Psest_1545 0.17 -1.4 1,644,770 - Psest_1545 0.17 +0.5 1,644,770 - Psest_1545 0.17 +0.0 1,644,856 + Psest_1545 0.21 -1.5 1,644,857 - Psest_1545 0.21 -0.2 1,644,872 + Psest_1545 0.22 -0.0 1,644,873 - Psest_1545 0.22 +0.3 1,644,873 - Psest_1545 0.22 -0.2 1,645,657 + Psest_1545 0.60 -1.9 1,645,657 + Psest_1545 0.60 -0.2 1,645,657 + Psest_1545 0.60 -1.0 1,645,658 - Psest_1545 0.60 -1.2 1,645,658 - Psest_1545 0.60 -1.6 1,645,658 - Psest_1545 0.60 -1.4 1,645,679 - Psest_1545 0.61 -1.8 1,645,679 - Psest_1545 0.61 -0.4 1,645,727 - Psest_1545 0.63 -0.7 1,645,727 - Psest_1545 0.63 -0.8 1,645,759 - Psest_1545 0.64 -0.2 1,645,995 - Psest_1545 0.76 +0.0 1,646,147 + Psest_1545 0.83 -0.5 1,646,147 + Psest_1545 0.83 +0.4 1,646,147 + Psest_1545 0.83 -0.3 1,646,147 + Psest_1545 0.83 +0.7 1,646,147 + Psest_1545 0.83 -1.0 1,646,147 + Psest_1545 0.83 -0.4 1,646,148 - Psest_1545 0.83 -2.7 1,646,176 + Psest_1545 0.84 -0.1 1,646,176 + Psest_1545 0.84 -0.7 1,646,301 - +0.7 1,646,301 - +0.8 1,646,301 - +0.7 1,646,451 - +1.2 1,646,588 - -0.2 1,646,792 + Psest_1546 0.17 -0.6 1,646,792 + Psest_1546 0.17 +1.4 1,646,792 + Psest_1546 0.17 +0.2 1,646,793 - Psest_1546 0.18 +0.6 1,646,793 - Psest_1546 0.18 +0.3 1,646,913 - Psest_1546 0.28 +0.9 1,646,913 - Psest_1546 0.28 +0.3 1,646,913 - Psest_1546 0.28 +1.4 1,647,124 + Psest_1546 0.47 +0.0 1,647,124 + Psest_1546 0.47 -0.5 1,647,124 + Psest_1546 0.47 -0.3 1,647,125 - Psest_1546 0.47 -0.6 1,647,125 - Psest_1546 0.47 +0.2
Or see this region's nucleotide sequence