Experiment: Def Cephalothin 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1036 and Psest_1037 are separated by 2 nucleotides Psest_1037 and Psest_1038 are separated by 76 nucleotides Psest_1038 and Psest_1039 are separated by 153 nucleotides
Psest_1036: Psest_1036 - acetolactate synthase, large subunit, biosynthetic type, at 1,121,522 to 1,123,246
_1036
Psest_1037: Psest_1037 - acetolactate synthase, small subunit, at 1,123,249 to 1,123,740
_1037
Psest_1038: Psest_1038 - ketol-acid reductoisomerase, at 1,123,817 to 1,124,833
_1038
Psest_1039: Psest_1039 - CDP-diacylglycerol--serine O-phosphatidyltransferase, at 1,124,987 to 1,125,826
_1039
Position (kb)
1123
1124
1125 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1123.512 kb on + strand, within Psest_1037 at 1123.512 kb on + strand, within Psest_1037 at 1123.596 kb on + strand, within Psest_1037 at 1123.608 kb on + strand, within Psest_1037 at 1123.727 kb on + strand at 1123.734 kb on + strand at 1123.734 kb on + strand at 1123.734 kb on + strand at 1123.734 kb on + strand at 1123.735 kb on - strand at 1123.735 kb on - strand at 1123.738 kb on + strand at 1123.747 kb on + strand at 1124.902 kb on + strand at 1124.962 kb on + strand at 1124.962 kb on + strand at 1124.962 kb on + strand at 1124.971 kb on + strand at 1124.971 kb on + strand at 1124.971 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Cephalothin 0.04 mg/ml remove 1,123,512 + Psest_1037 0.53 -3.1 1,123,512 + Psest_1037 0.53 -3.8 1,123,596 + Psest_1037 0.71 -3.5 1,123,608 + Psest_1037 0.73 -0.9 1,123,727 + -0.7 1,123,734 + +0.7 1,123,734 + +0.6 1,123,734 + -1.7 1,123,734 + -1.7 1,123,735 - +0.7 1,123,735 - +0.7 1,123,738 + -2.3 1,123,747 + -0.0 1,124,902 + -2.1 1,124,962 + +0.3 1,124,962 + +1.7 1,124,962 + -2.7 1,124,971 + -0.9 1,124,971 + -2.3 1,124,971 + -0.4
Or see this region's nucleotide sequence