Experiment: Def Vancomycin 0.016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0091 and Psest_0092 are separated by 11 nucleotides Psest_0092 and Psest_0093 overlap by 4 nucleotides Psest_0093 and Psest_0094 overlap by 4 nucleotides
Psest_0091: Psest_0091 - phosphonate C-P lyase system protein PhnL, at 92,087 to 92,791
_0091
Psest_0092: Psest_0092 - phosphonate C-P lyase system protein PhnK, at 92,803 to 93,615
_0092
Psest_0093: Psest_0093 - Uncharacterized enzyme of phosphonate metabolism, at 93,612 to 94,481
_0093
Psest_0094: Psest_0094 - Uncharacterized enzyme of phosphonate metabolism, at 94,478 to 95,587
_0094
Position (kb)
93
94
95 Strain fitness (log2 ratio)
-1
0
1
2 at 92.620 kb on - strand, within Psest_0091 at 92.838 kb on + strand at 92.839 kb on - strand at 92.839 kb on - strand at 92.840 kb on + strand at 93.386 kb on + strand, within Psest_0092 at 93.715 kb on + strand, within Psest_0093 at 93.715 kb on + strand, within Psest_0093 at 93.715 kb on + strand, within Psest_0093 at 93.809 kb on - strand, within Psest_0093 at 93.809 kb on - strand, within Psest_0093 at 93.923 kb on - strand, within Psest_0093 at 93.952 kb on + strand, within Psest_0093 at 93.953 kb on - strand, within Psest_0093 at 94.096 kb on + strand, within Psest_0093 at 94.096 kb on + strand, within Psest_0093 at 94.096 kb on + strand, within Psest_0093 at 94.444 kb on + strand at 94.444 kb on + strand at 94.584 kb on + strand at 94.584 kb on + strand at 94.584 kb on + strand at 94.584 kb on + strand at 94.585 kb on - strand at 94.585 kb on - strand at 94.585 kb on - strand at 94.585 kb on - strand at 95.217 kb on + strand, within Psest_0094 at 95.217 kb on + strand, within Psest_0094 at 95.218 kb on - strand, within Psest_0094 at 95.218 kb on - strand, within Psest_0094 at 95.218 kb on - strand, within Psest_0094
Per-strain Table
Position Strand Gene LocusTag Fraction Def Vancomycin 0.016 mg/ml remove 92,620 - Psest_0091 0.76 +0.9 92,838 + +0.9 92,839 - -0.1 92,839 - -0.7 92,840 + +0.1 93,386 + Psest_0092 0.72 -0.7 93,715 + Psest_0093 0.12 -0.7 93,715 + Psest_0093 0.12 -0.9 93,715 + Psest_0093 0.12 -0.4 93,809 - Psest_0093 0.23 -0.9 93,809 - Psest_0093 0.23 -0.1 93,923 - Psest_0093 0.36 +0.3 93,952 + Psest_0093 0.39 +0.9 93,953 - Psest_0093 0.39 +0.3 94,096 + Psest_0093 0.56 +1.9 94,096 + Psest_0093 0.56 +1.9 94,096 + Psest_0093 0.56 +0.6 94,444 + -0.7 94,444 + +0.9 94,584 + +0.3 94,584 + +1.3 94,584 + -0.2 94,584 + +1.3 94,585 - +1.9 94,585 - -1.4 94,585 - -0.1 94,585 - +0.3 95,217 + Psest_0094 0.67 +0.3 95,217 + Psest_0094 0.67 +0.3 95,218 - Psest_0094 0.67 -0.4 95,218 - Psest_0094 0.67 +1.9 95,218 - Psest_0094 0.67 -0.7
Or see this region's nucleotide sequence