Experiment: Def Vancomycin 0.016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3370 and Psest_3371 overlap by 4 nucleotides Psest_3371 and Psest_3372 overlap by 4 nucleotides Psest_3372 and Psest_3373 overlap by 4 nucleotides Psest_3373 and Psest_3374 are separated by 4 nucleotides
Psest_3370: Psest_3370 - prepilin-type N-terminal cleavage/methylation domain, at 3,539,223 to 3,539,714
_3370
Psest_3371: Psest_3371 - type IV pilus modification protein PilV, at 3,539,711 to 3,540,181
_3371
Psest_3372: Psest_3372 - Tfp pilus assembly protein PilW, at 3,540,178 to 3,541,251
_3372
Psest_3373: Psest_3373 - Tfp pilus assembly protein PilX, at 3,541,248 to 3,541,781
_3373
Psest_3374: Psest_3374 - Tfp pilus assembly protein, tip-associated adhesin PilY1, at 3,541,786 to 3,545,556
_3374
Position (kb)
3540
3541
3542 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3539.278 kb on + strand, within Psest_3370 at 3539.278 kb on + strand, within Psest_3370 at 3539.279 kb on - strand, within Psest_3370 at 3539.324 kb on - strand, within Psest_3370 at 3539.361 kb on - strand, within Psest_3370 at 3539.361 kb on - strand, within Psest_3370 at 3539.361 kb on - strand, within Psest_3370 at 3539.377 kb on + strand, within Psest_3370 at 3539.378 kb on - strand, within Psest_3370 at 3539.378 kb on - strand, within Psest_3370 at 3539.378 kb on - strand, within Psest_3370 at 3539.378 kb on - strand, within Psest_3370 at 3539.485 kb on + strand, within Psest_3370 at 3539.486 kb on - strand, within Psest_3370 at 3539.561 kb on + strand, within Psest_3370 at 3539.561 kb on + strand, within Psest_3370 at 3539.561 kb on + strand, within Psest_3370 at 3539.561 kb on + strand, within Psest_3370 at 3539.561 kb on + strand, within Psest_3370 at 3539.561 kb on + strand, within Psest_3370 at 3539.562 kb on - strand, within Psest_3370 at 3539.562 kb on - strand, within Psest_3370 at 3539.562 kb on - strand, within Psest_3370 at 3539.562 kb on - strand, within Psest_3370 at 3539.562 kb on - strand, within Psest_3370 at 3539.562 kb on - strand, within Psest_3370 at 3539.562 kb on - strand, within Psest_3370 at 3539.562 kb on - strand, within Psest_3370 at 3539.562 kb on - strand, within Psest_3370 at 3539.562 kb on - strand, within Psest_3370 at 3539.562 kb on - strand, within Psest_3370 at 3539.562 kb on - strand, within Psest_3370 at 3539.562 kb on - strand, within Psest_3370 at 3539.562 kb on - strand, within Psest_3370 at 3539.724 kb on + strand at 3539.724 kb on + strand at 3539.725 kb on - strand at 3539.766 kb on + strand, within Psest_3371 at 3539.766 kb on + strand, within Psest_3371 at 3539.766 kb on + strand, within Psest_3371 at 3539.766 kb on + strand, within Psest_3371 at 3539.767 kb on - strand, within Psest_3371 at 3539.852 kb on + strand, within Psest_3371 at 3539.852 kb on + strand, within Psest_3371 at 3539.853 kb on - strand, within Psest_3371 at 3539.853 kb on - strand, within Psest_3371 at 3539.853 kb on - strand, within Psest_3371 at 3539.855 kb on + strand, within Psest_3371 at 3539.855 kb on + strand, within Psest_3371 at 3539.869 kb on + strand, within Psest_3371 at 3539.874 kb on + strand, within Psest_3371 at 3539.883 kb on - strand, within Psest_3371 at 3539.883 kb on - strand, within Psest_3371 at 3539.936 kb on + strand, within Psest_3371 at 3539.936 kb on + strand, within Psest_3371 at 3539.937 kb on - strand, within Psest_3371 at 3539.937 kb on - strand, within Psest_3371 at 3540.177 kb on + strand at 3540.178 kb on - strand at 3540.227 kb on + strand at 3540.228 kb on - strand at 3540.228 kb on - strand at 3540.270 kb on - strand at 3540.270 kb on - strand at 3540.301 kb on + strand, within Psest_3372 at 3540.301 kb on + strand, within Psest_3372 at 3540.301 kb on + strand, within Psest_3372 at 3540.301 kb on + strand, within Psest_3372 at 3540.301 kb on + strand, within Psest_3372 at 3540.302 kb on - strand, within Psest_3372 at 3540.302 kb on - strand, within Psest_3372 at 3540.312 kb on + strand, within Psest_3372 at 3540.313 kb on - strand, within Psest_3372 at 3540.463 kb on + strand, within Psest_3372 at 3540.464 kb on - strand, within Psest_3372 at 3540.566 kb on - strand, within Psest_3372 at 3540.566 kb on - strand, within Psest_3372 at 3540.566 kb on - strand, within Psest_3372 at 3540.645 kb on + strand, within Psest_3372 at 3540.653 kb on - strand, within Psest_3372 at 3540.794 kb on - strand, within Psest_3372 at 3540.804 kb on + strand, within Psest_3372 at 3540.836 kb on - strand, within Psest_3372 at 3540.836 kb on - strand, within Psest_3372 at 3540.836 kb on - strand, within Psest_3372 at 3540.836 kb on - strand, within Psest_3372 at 3540.836 kb on - strand, within Psest_3372 at 3540.985 kb on + strand, within Psest_3372 at 3540.986 kb on - strand, within Psest_3372 at 3540.989 kb on + strand, within Psest_3372 at 3540.990 kb on - strand, within Psest_3372 at 3540.990 kb on - strand, within Psest_3372 at 3541.011 kb on - strand, within Psest_3372 at 3541.038 kb on + strand, within Psest_3372 at 3541.162 kb on + strand at 3541.310 kb on + strand, within Psest_3373 at 3541.311 kb on - strand, within Psest_3373 at 3541.581 kb on + strand, within Psest_3373 at 3541.581 kb on + strand, within Psest_3373 at 3541.581 kb on + strand, within Psest_3373 at 3541.581 kb on + strand, within Psest_3373 at 3541.582 kb on - strand, within Psest_3373 at 3541.699 kb on + strand, within Psest_3373 at 3541.700 kb on - strand, within Psest_3373 at 3541.700 kb on - strand, within Psest_3373 at 3541.731 kb on - strand at 3541.764 kb on + strand at 3541.765 kb on - strand at 3541.847 kb on + strand at 3541.920 kb on - strand at 3541.940 kb on + strand at 3542.011 kb on + strand at 3542.030 kb on - strand at 3542.128 kb on + strand at 3542.129 kb on - strand at 3542.134 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Vancomycin 0.016 mg/ml remove 3,539,278 + Psest_3370 0.11 -0.3 3,539,278 + Psest_3370 0.11 -1.7 3,539,279 - Psest_3370 0.11 -2.9 3,539,324 - Psest_3370 0.21 -3.9 3,539,361 - Psest_3370 0.28 -3.0 3,539,361 - Psest_3370 0.28 -0.1 3,539,361 - Psest_3370 0.28 -1.4 3,539,377 + Psest_3370 0.31 -1.2 3,539,378 - Psest_3370 0.32 -2.2 3,539,378 - Psest_3370 0.32 -1.0 3,539,378 - Psest_3370 0.32 -1.3 3,539,378 - Psest_3370 0.32 -1.2 3,539,485 + Psest_3370 0.53 -1.3 3,539,486 - Psest_3370 0.53 -3.2 3,539,561 + Psest_3370 0.69 -1.5 3,539,561 + Psest_3370 0.69 +0.5 3,539,561 + Psest_3370 0.69 -1.3 3,539,561 + Psest_3370 0.69 +0.1 3,539,561 + Psest_3370 0.69 +0.8 3,539,561 + Psest_3370 0.69 +1.7 3,539,562 - Psest_3370 0.69 -0.6 3,539,562 - Psest_3370 0.69 -1.5 3,539,562 - Psest_3370 0.69 -2.0 3,539,562 - Psest_3370 0.69 -1.0 3,539,562 - Psest_3370 0.69 -1.6 3,539,562 - Psest_3370 0.69 -1.8 3,539,562 - Psest_3370 0.69 -2.2 3,539,562 - Psest_3370 0.69 -1.4 3,539,562 - Psest_3370 0.69 -1.4 3,539,562 - Psest_3370 0.69 -0.9 3,539,562 - Psest_3370 0.69 -2.5 3,539,562 - Psest_3370 0.69 -1.5 3,539,562 - Psest_3370 0.69 -1.1 3,539,562 - Psest_3370 0.69 -1.2 3,539,724 + -1.3 3,539,724 + -0.8 3,539,725 - -1.8 3,539,766 + Psest_3371 0.12 -0.9 3,539,766 + Psest_3371 0.12 -2.5 3,539,766 + Psest_3371 0.12 -1.5 3,539,766 + Psest_3371 0.12 -2.5 3,539,767 - Psest_3371 0.12 -1.0 3,539,852 + Psest_3371 0.30 -0.9 3,539,852 + Psest_3371 0.30 -1.5 3,539,853 - Psest_3371 0.30 -0.8 3,539,853 - Psest_3371 0.30 +0.7 3,539,853 - Psest_3371 0.30 -1.4 3,539,855 + Psest_3371 0.31 -2.9 3,539,855 + Psest_3371 0.31 -2.0 3,539,869 + Psest_3371 0.34 -1.5 3,539,874 + Psest_3371 0.35 -3.0 3,539,883 - Psest_3371 0.37 -1.5 3,539,883 - Psest_3371 0.37 +0.4 3,539,936 + Psest_3371 0.48 -1.1 3,539,936 + Psest_3371 0.48 -1.2 3,539,937 - Psest_3371 0.48 -1.8 3,539,937 - Psest_3371 0.48 -1.2 3,540,177 + -1.5 3,540,178 - -0.6 3,540,227 + -1.6 3,540,228 - -0.9 3,540,228 - -2.3 3,540,270 - -1.9 3,540,270 - -2.1 3,540,301 + Psest_3372 0.11 -0.6 3,540,301 + Psest_3372 0.11 -1.8 3,540,301 + Psest_3372 0.11 -1.2 3,540,301 + Psest_3372 0.11 -2.0 3,540,301 + Psest_3372 0.11 -1.3 3,540,302 - Psest_3372 0.12 -1.1 3,540,302 - Psest_3372 0.12 +0.7 3,540,312 + Psest_3372 0.12 -3.2 3,540,313 - Psest_3372 0.13 -1.0 3,540,463 + Psest_3372 0.27 -1.5 3,540,464 - Psest_3372 0.27 -1.5 3,540,566 - Psest_3372 0.36 -2.9 3,540,566 - Psest_3372 0.36 -0.3 3,540,566 - Psest_3372 0.36 -2.5 3,540,645 + Psest_3372 0.43 -2.5 3,540,653 - Psest_3372 0.44 -2.3 3,540,794 - Psest_3372 0.57 -1.2 3,540,804 + Psest_3372 0.58 -1.5 3,540,836 - Psest_3372 0.61 -1.6 3,540,836 - Psest_3372 0.61 -1.6 3,540,836 - Psest_3372 0.61 -1.2 3,540,836 - Psest_3372 0.61 +1.1 3,540,836 - Psest_3372 0.61 -2.3 3,540,985 + Psest_3372 0.75 -0.3 3,540,986 - Psest_3372 0.75 -1.5 3,540,989 + Psest_3372 0.76 -1.4 3,540,990 - Psest_3372 0.76 -1.3 3,540,990 - Psest_3372 0.76 -1.5 3,541,011 - Psest_3372 0.78 -1.0 3,541,038 + Psest_3372 0.80 -2.2 3,541,162 + -2.5 3,541,310 + Psest_3373 0.12 -2.9 3,541,311 - Psest_3373 0.12 -2.1 3,541,581 + Psest_3373 0.62 -1.4 3,541,581 + Psest_3373 0.62 -1.4 3,541,581 + Psest_3373 0.62 -2.7 3,541,581 + Psest_3373 0.62 -1.4 3,541,582 - Psest_3373 0.63 -1.6 3,541,699 + Psest_3373 0.84 -1.8 3,541,700 - Psest_3373 0.85 -1.6 3,541,700 - Psest_3373 0.85 -1.3 3,541,731 - -1.5 3,541,764 + -0.9 3,541,765 - -1.4 3,541,847 + -2.5 3,541,920 - -0.6 3,541,940 + -2.0 3,542,011 + -1.5 3,542,030 - -1.5 3,542,128 + -0.3 3,542,129 - -1.3 3,542,134 - -0.3
Or see this region's nucleotide sequence